High-Rate Nitrification Using Aerobic Granular Sludge

2006 ◽  
Vol 53 (3) ◽  
pp. 147-154 ◽  
Author(s):  
S. Tsuneda ◽  
M. Ogiwara ◽  
Y. Ejiri ◽  
A. Hirata

The performance of nitrifying granules, which had been produced in an aerobic upflow fluidised bed (AUFB) reactor, was investigated in various types of ammonia-containing wastewaters. When pure oxygen was supplied to the AUFB reactor with a synthetic wastewater containing a high concentration of ammonia (500 g-N/m3), the ammonia removal rate reached 16.7 kg-N/m3/day with a sustained ammonia removal efficiency of more than 80%. The nitrifying granules possessing a high settling ability could be retained with a high density (approximately 10,000 g-MLSS/m3) in a continuous stirring tank reactor (CSTR) even under a short hydraulic retention time (44 min), which enabled a high-rate and stable nitrification for an inorganic wastewater containing low concentrations of ammonia (50 g-N/m3). Moreover, the nitrifying granules exhibited sufficient performance in the nitrification of real industrial wastewater containing high concentrations of ammonia (1,000–1,400 g-N/m3) and salinity (1.2–2.2%), which was discharged from metal-refinery processes. When the nitrifying granules were used in cooperation with activated sludge to treat domestic wastewater containing organic pollutants as well as ammonia, they fully contributed to nitrification even though a part of activated sludge adhered onto the granule surfaces to form biofilms. These results show the wide applicability of nitrifying granules to various cases in the nitrification step of wastewater treatment plants.

2012 ◽  
Vol 65 (3) ◽  
pp. 581-588 ◽  
Author(s):  
Naohiro Kishida ◽  
Goro Saeki ◽  
Satoshi Tsuneda ◽  
Ryuichi Sudo

In this study, the effectiveness of aerobic granular sludge as seed sludge for rapid start-up of nitrifying processes was investigated using a laboratory-scale continuous stirred-tank reactor (CSTR) fed with completely inorganic wastewater which contained a high concentration of ammonia. Even when a large amount of granular biomass was inoculated in the reactor, and the characteristics of influent wastewater were abruptly changed, excess biomass washout was not observed, and biomass concentration was kept high at the start-up period due to high settling ability of the aerobic granular sludge. As a result, an ammonia removal rate immediately increased and reached more than 1.0 kg N/m3/d within 20 days and up to 1.8 kg N/m3/d on day 39. Subsequently, high rate nitritation was stably attained during 100 days. However, nitrite accumulation had been observed for 140 days before attaining complete nitrification to nitrate. Fluorescence in situ hybridization analysis revealed the increase in amount of ammonia-oxidizing bacteria which existed in the outer edge of the granular sludge during the start-up period. This microbial ecological change would make it possible to attain high rate ammonia removal.


2011 ◽  
Vol 356-360 ◽  
pp. 1267-1271
Author(s):  
Feng Jiang ◽  
Yang Li Zhao ◽  
Shuai Fu Chen ◽  
Rui Liang

Aerobic granular sludge (AGS) was cultivated successfully in a sequencing batch reactor (SBR), which has floc sludge as seeding sludge in synthetic wastewater. Removal efficiency in the reactor during the entire working period was investigated. Controlling strategy for filamentous bulking and the effect of a filamentous microorganism on granulation of sludge were discussed. The results showed that whether filamentous bulking occurred or not, there was little impact on the removal rate of phosphorus and ammonia nitrogen, which maintain about 90% and 95%, respectively. Increasing the hydrodynamic shear force has certain effects on the controlling of filamentous bulking. Declining C/N ratio, balancing nutrition in feed water can fundamentally solve the problem of filamentous bulking in the cultivation process of aerobic granular sludge.


2017 ◽  
Vol 76 (11) ◽  
pp. 3171-3180 ◽  
Author(s):  
Yoshiaki Hasebe ◽  
Hiroaki Meguro ◽  
Yuuki Kanai ◽  
Masahiro Eguchi ◽  
Toshifumi Osaka ◽  
...  

Abstract Nitrifying granules have a high sedimentation property and an ability to maintain a large amount of nitrifying bacteria in a reaction tank. Our group has examined the formation process of nitrifying granules and achieved high-rate nitrification for an inorganic synthetic wastewater using these granules. In this research, a pilot-scale test plant with an 850-liter reaction tank was assembled in a semiconductor manufacturing factory in order to conduct a continuous water conduction test using real electronics industry wastewater. The aim was to observe the formation of nitrifying granules and determine the maximum ammonia removal rate. The average granule diameter formed during the experiment was 780 μm and the maximum ammonia removal rate was observed to be 1.5 kgN·m−3·day−1 at 20 °C, which is 2.5–5 times faster than traditional activated sludge methods. A fluorescence in situ hybridization analysis showed that β-proteobacterial ammonia oxidizing bacteria and the Nitrospira-like nitrite-oxidizing bacteria dominate the bacteria population in the granules, and their strong aggregation capacity might confer some benefits to the formation of these nitrifying granules.


2014 ◽  
Vol 69 (11) ◽  
pp. 2252-2257 ◽  
Author(s):  
Hasnida Harun ◽  
Aznah Nor Anuar ◽  
Zaini Ujang ◽  
Noor Hasyimah Rosman ◽  
Inawati Othman

Aerobic granular sludge (AGS) has been applied to treat a broad range of industrial and municipal wastewater. AGS can be developed in a sequencing batch reactor (SBR) with alternating anaerobic–aerobic conditions. To provide anaerobic conditions, the mixed liquor is allowed to circulate in the reactor without air supply. The circulation flow rate of mixed liquor in anaerobic condition is the most important parameter of operation in the anaerobic-AGS processes. Therefore, this study investigates the effect of circulation rate on the performance of the SBR with AGS. Two identical reactors namely R1 and R2 were operated using fermented soy sauce wastewater at circulation rate of 14.4 and 36.0 l/h, respectively. During the anaerobic conditions, the wastewater was pumped out from the upper part of the reactor and circulated back into the bottom of the reactor for 230 min. A compact and dense AGS was observed in both reactors with a similar diameter of 2.0 mm in average, although different circulation rates were adopted. The best reactor performance was achieved in R2 with chemical oxygen demand removal rate of 89%, 90% total phosphorus removal, 79% ammonia removal, 10.1 g/l of mixed liquor suspended solids and a sludge volume index of 25 ml/g.


2014 ◽  
Vol 54 ◽  
pp. 337-346 ◽  
Author(s):  
Adriana Maria Lotito ◽  
Marco De Sanctis ◽  
Claudio Di Iaconi ◽  
Giovanni Bergna

1992 ◽  
Vol 26 (3-4) ◽  
pp. 601-605 ◽  
Author(s):  
H.-S. Shin ◽  
K.-H. Lim ◽  
H.-S. Park

Aerobic upflow sludge blanket(AUSB) process is a new biological wastewater treatment method applying the concept of the self-immobilization to activated sludge. Two sets of AUSB system with different mixing velocities of 3 rpm(R1) and 6 rpm(R2) were operated for high-rate treatment of synthetic wastewater. The COD removal efficiency in R2 was higher than R1 at the same loading rate up to 7 kg/m3·day. However, in R1, the sludge bulking was observed at the end of the experiment. The chocolate colored granules were formed about 5 days after the start-up. The morphological study on the granular sludge consortia was made with both scanning electron and optical microscopes. The granules were 0.5-2.5 mm in diameter and mainly consisted of bacteria with pili-like appendages and filamentous bacteria, which were thought to be Sphaerotilus natans and Beggiatoa. In R1, the long multicellular filaments causing bulking were prevalent in the granule, while in R2 overgrowth of filamentous bacteria was prevented with appropriate shear stress resulting in higher MLSS density. Experimental results indicated that granulation could be controlled by physical stress on granular sludge.


2019 ◽  
Vol 131 ◽  
pp. 01129
Author(s):  
Xiaoying Zheng ◽  
Xiaoyao Shao ◽  
Yuan Zhang ◽  
Mengmeng Yang ◽  
Zhi Xu ◽  
...  

With the increasing use of cupric oxide nanoparticles (CuO NPs), its potential environmental toxicity has been concerned nowadays. Aerobic granular sludge (AGS) is a special collection of microorganisms. This research studied under long exposure to the concentration of 5, 10 and 20 mg/L of CuO NPs, pollutants removal efficiency of AGS, extracellular polymers (EPS) and microbial communities in aerobic/anaerobic/anoxic (A/O/A) sequencing batch reactors (SBRs). The results showed that COD removal rates was stable, and the removal efficiencies of TN decreased because of the high concentration CuO NPs. On the 45th day, the TP removal efficiency of the reactor with CuO NPs concentration of 10 mg/L and 20 mg/L decreased to 55.83% and 43.72%, respectively. The denitrifying phosphorus removal-aerobic granular sludge (DPR-AGS) had certain resistance to the short-term impact of CuO NPs, and the phosphorus removal ability decreased at the late stage of the impact test. Besides, CuO NPs decreased the stability of DPR-AGS. High-throughput sequencing showed that CuO NPs decreased microbial diversity of DPR-AGS.


2004 ◽  
Vol 50 (10) ◽  
pp. 1-10 ◽  
Author(s):  
P.A. Wilderer ◽  
B.S. McSwain

Twenty plus years of experience, innovation, and research in the field of biological wastewater treatment and biofilm applications lead to the conclusion that biofilms are in many cases more desirable in reactors than suspended activated sludge. Biofilm reactors can provide very long biomass residence times even when the hydraulic influent loading is low. This makes them particularly suitable when treatment requires slow growing organisms with poor biomass yield or when the wastewater concentration is too low to support growth of activated sludge flocs. Regardless of the settling characteristics of biological aggregates or the hydraulic influent loading the metabolic activity in the reactor can be maintained at a high level. This paper reviews the application of biofilms in sequencing batch reactor (SBR) systems to treat non-readily biodegradable substrates, volatile organic waste constituents, complex waste streams requiring co-metabolism, and particulate wastewaters. Recent research using the SBR to form aerobic granular sludge as a special application of biofilms is also discussed.


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