Application of biofilm reactors to improve ammonia oxidation in low nitrogen loaded wastewater

2011 ◽  
Vol 63 (9) ◽  
pp. 1880-1886 ◽  
Author(s):  
I. Seca ◽  
R. Torres ◽  
A. Val del Río ◽  
A. Mosquera-Corral ◽  
J. L. Campos ◽  
...  

An airlift reactor using zeolite particles as carrier material was used for the nitrification of effluents from the aquaculture industry. During the start-up the nitrogen concentration was kept around 100 mg NH4+-N/L to develop the nitrifying population. Later it was decreased down to around 3 mg NH4+-N/L and the dilution rate was increased up to 4.8 d−1 in order to simulate the conditions in a an aquaculture waster treatment system. A nitrogen loading rate (NLR) of 535 mg NH4+-N/m2 d was fully oxidized to nitrate. Higher values of NLRs caused nitrite accumulation. A second biofilm reactor was fed with a synthetic medium containing 50 mg NH4+-N/L which simulated the effluents from anaerobic units treating domestic wastewater. A nitrogen loading rate of 400 mg NH4+-N/L d was oxidized into nitrate with an efficiency of 60% at a dilution rate of 8 d−1. Both biofilm systems allowed the development of a nitrifying population to treat the studied types of wastewaters.

2013 ◽  
Vol 69 (6) ◽  
pp. 1227-1233 ◽  
Author(s):  
Vojtech Kouba ◽  
Michael Catrysse ◽  
Hana Stryjova ◽  
Ivana Jonatova ◽  
Eveline I. P. Volcke ◽  
...  

The application of nitrification–denitrification over nitrite (nitritation–denitritation) with municipal (i.e. diluted and cold (or low-temperature)) wastewater can substantially improve the energy balance of municipal wastewater treatment plants. For the accumulation of nitrite, it is crucial to inhibit nitrite-oxidizing bacteria (NOB) with simultaneous proliferation of ammonium-oxidizing bacteria (AOB). The present study describes the effect of the influent total ammonium nitrogen (TAN) concentration on AOB and NOB activity in two moving bed biofilm reactors operated as sequencing batch reactors (SBR) at 15 °C (SBR I) and 21 °C (SBR II). The reactors were fed with diluted reject water containing 600, 300, 150 and 75 mg TAN L−1. The only factor limiting NOB activity in these reactors was the high concentrations of free ammonia and/or free nitrous acid (FNA) during the SBR cycles. Nitrite accumulation was observed with influents containing 600, 300 and 150 mg TAN L−1 in SBR I and 600 and 300 in SBR II. Once nitrate production established in the reactors, the increase of influent TAN concentration up to the original 600 mg TAN L−1 did not limit NOB activity. This was due to the massive development of NOB clusters throughout the biofilm that were able to cope with faster formation of FNA. The results of the fluorescence in situ hybridization analysis preliminarily showed the stratification of bacteria in the biofilm.


2012 ◽  
Vol 2 (3) ◽  
pp. 165-174 ◽  
Author(s):  
M. F. Hamoda ◽  
R. A. Bin-Fahad

A pilot plant, using a four-compartment reactor packed with Biolace media, was operated in the anoxic/aerobic submerged fixed-film (A/ASFF) and the aerobic (ASFF) modes at loadings 0.03 to 0.3 g BOD. g−1 BVS. d−1, 0.01 to 0.11 g NH3. g−1 BVS d−1, HRTs 0.7 to 8 h, C/N of 6, and 28 ± 2 °C. The system proved to be very effective in treating municipal wastewater, achieving removals up to 98% for biological oxygen demand (BOD), 75% for chemical oxygen demand (COD) and 97% for ammonia. Performance was not adversely affected by a 10-fold increase in loading rate. Both modes of operation showed high specific nitrification rates up to 96 mg N. g−1 BVS d−1, but the A/ASFF was more stable and efficient at higher loadings. Its anoxic stage removed more than 90 and 60% for BOD and COD, respectively. The A/ASFF reactor also achieved denitrification, which eliminated 3.35 mg BOD (or 6.6 mg COD) versus 1 mg denitrified NO3-N, that resulted in higher organic removals. Denitrification rate increased linearly with the TON (total oxidised nitrogen) loading applied, and specific substrate removal reached up to 114 mg TON. g−1 BVS. d−1.


2013 ◽  
Vol 3 (1) ◽  
Author(s):  
BURHANETTIN GURBUZ

Short-cut nitrification under various Nitrogen Loading Rate (NLR), dissolved oxygen (DO) concentrations and pHs at a constant temperature of 25 ±1 0C was investigated in a submerged biofilter reactor. The lowest NO2-N/NOx-N ratio was observed when the NLR was up to 160 g/m3·day. Further increase the NLRresulted in incomplete oxidation of ammonium and the effluent NH4-N and NO2-N concentration increased. Although the highest NO2-N/NOx-N ratio of 0.61 was achieved, the NH4-N removal efficiency was drastically dropped to 48% at the NLR of 294 g NH4-N/m3.day. The highest NO2-N/NOx-N ratio of 0.68 was achieved at the pH of 9.0 and 1.0 mg O2/l. However, NN4-N removal efficiency and the NO2-N/NOx-N ratio were sharply dropped to 33% and 0.55 by getting the NLR to 381 g NH4-N/m3⋅day at the DO concentrations of 2.0 mg/l. Key words: partial nitrification, NO2-N/NOx-N ratio.


Water ◽  
2020 ◽  
Vol 12 (2) ◽  
pp. 492 ◽  
Author(s):  
Gaetano Di Bella ◽  
Giorgio Mannina

The paper presents an experimental study on a lab scale hybrid moving bed biofilm reactor with intermittent aeration. Specifically, a comparison between two different operating conditions was analyzed: continuous and intermittent aeration. Both continuous and intermittent aeration were monitored and compared in order to get the best operational conditions. The intermittent aeration campaign was sub-divided in three phases with different duration of alternation of aerobic and anoxic times and organic and nitrogen loading rates. The efficiency of N-removal improved by 70% during the intermittent aeration. The best condition was observed with 40 min of aeration and 20 min of no-aeration, an organic loading rate of 2.2 kgCODm−3day−1 and a nitrogen loading rate of 0.25 kgNm−3day−1: under these operational conditions the removal efficiencies for carbon and nitrogen were 93% and 90%, respectively. The derived results provide the basis for WWTP upgrade in order to meet stricter effluent limits at low energy requirements.


2014 ◽  
Vol 955-959 ◽  
pp. 2318-2321
Author(s):  
Dong Yuan

The objective of this work was to evaluate the performances of A lab-scale innovative sequencing batch biofilm reactor (SBBR) to treat domestic wastewater,in which a acryl cylinder (height 200 mm, diameter 70 mm) was equipped and many fiber threads were attached to the surface of the cylinder as the bacteria carrier. No time and volume for settling was required in this system. After one year’s operation, each parameter achieved the wastewater discharged criterion in 2 cycles (4 h). It was found that COD removal efficiency was up to 90% in 3 h, and ammonium nitrogen concentration approached the least value; total nitrogen removal efficiency reached 55%-71%. In this SBBR system simultaneous nitrification and denitrification was completed at the end of 2 cycles.


RSC Advances ◽  
2014 ◽  
Vol 4 (97) ◽  
pp. 54798-54804 ◽  
Author(s):  
Ying-Cui Yu ◽  
Yu Tao ◽  
Da-Wen Gao

There are three key aspects of substrate effect on anaerobic ammonia oxidizing (anammox) bacteria: (1) substrate concentration-based nitrogen loading rate (NLR), (2) hydraulic retention time (HRT)-based NLR and (3) nitrite/ammonia ratio.


2004 ◽  
Vol 50 (10) ◽  
pp. 35-43 ◽  
Author(s):  
Y.Z. Peng ◽  
Y. Chen ◽  
C.Y. Peng ◽  
M. Liu ◽  
S.Y. Wang ◽  
...  

The feasibility of obtaining and keeping stable nitrite accumulation in Sequencing Batch Reactors (SBRs) treating domestic wastewater is studied. The final product of ammonium oxidation is either reproducible nitrate or nitrite depending on the aeration strategy. With the aerobic-anoxic sequence, two SBRs fed with domestic wastewater are operated in parallel. One SBR (SBR1) is controlled by the aeration control strategy, and the other SBR (SBR2) by alternate aeration control strategy. Based on the on-line indirect measurements of DO and pH, the relationship between pH (or DO) and nitrogen concentration (NH4+-N, NO3−-N and NO2−-N) is investigated. The result indicates that pH and DO can be used as control parameters for the real-time aeration control strategy to obtain nitritation in SBR treating domestic wastewater. The result of SBR1 indicates that long-term stable nitritation is possible at 32 ± 1°C. The result of SBR2 indicates that the aeration control strategy is necessary for nitritation during the acclimation period, because the nitrite accumulation disappears when the aeration is extended.


2000 ◽  
Vol 41 (4-5) ◽  
pp. 487-492 ◽  
Author(s):  
D. Pak ◽  
W. Chang

A two-biofilter system operated under alternating anaerobic/aerobic conditions was tested to remove nutrient as well as organics from wastewater generated from car-washing facility. The wastewater was characterized by relatively low organic and high phosphorus content. The factors affecting phosphorus removal in the two-biofilter system were investigated. Operational parameters examined in this study were hydraulic retention time, organic, suspended solid and nitrogen loading rate. The factors affecting phosphorus removal in biological filter appeared to be influent COD, COD/T–P, BOD/COD, nitrogen, and SS/T–P. Nitrite and nitrate produced in the biofilter in aerobic condition affected phosphorus removal by the two-biofilter system. The biomass wasted during backwash procedure also affected total phosphorus removal by the system.


2016 ◽  
Vol 75 (1) ◽  
pp. 57-68 ◽  
Author(s):  
Takahiro Watari ◽  
Trung Cuong Mai ◽  
Daisuke Tanikawa ◽  
Yuga Hirakata ◽  
Masashi Hatamoto ◽  
...  

Conventional aerated tank technology is widely applied for post treatment of natural rubber processing wastewater in Southeast Asia; however, a long hydraulic retention time (HRT) is required and the effluent standards are exceeded. In this study, a downflow hanging sponge (DHS) reactor was installed as post treatment of anaerobic tank effluent in a natural rubber factory in South Vietnam and the process performance was evaluated. The DHS reactor demonstrated removal efficiencies of 64.2 ± 7.5% and 55.3 ± 19.2% for total chemical oxygen demand (COD) and total nitrogen, respectively, with an organic loading rate of 0.97 ± 0.03 kg-COD m−3 day−1 and a nitrogen loading rate of 0.57 ± 0.21 kg-N m−3 day−1. 16S rRNA gene sequencing analysis of the sludge retained in the DHS also corresponded to the result of reactor performance, and both nitrifying and denitrifying bacteria were detected in the sponge carrier. In addition, anammox bacteria was found in the retained sludge. The DHS reactor reduced the HRT of 30 days to 4.8 h compared with the existing algal tank. This result indicates that the DHS reactor could be an appropriate post treatment for the existing anaerobic tank for natural rubber processing wastewater treatment.


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