Characteristics of estrogen decomposition by ozonation

2006 ◽  
Vol 54 (10) ◽  
pp. 87-93 ◽  
Author(s):  
T. Hashimoto ◽  
K. Takahashi ◽  
T. Murakami
Keyword(s):  
Wastewater Treatment ◽  
Wastewater Treatment Plants ◽  
Estrogenic Activity ◽  
Removal Rate ◽  
Treatment Plant ◽  
Treated Wastewater ◽  
Ozone Dose ◽  
Natural Estrogens ◽  
Ethynyl Estradiol ◽  
Estrogen Concentration

Since the natural estrogens 17 β-estradiol (E2) and estron (E1), and the synthetic estrogen 17 α-ethynyl estradiol (EE2) have strong endocrine disrupting effects and the tendency to persist in effluent from wastewater treatment plants, effective measures are needed to remove them from wastewater. In this research, to gain an understanding of the characteristics of estrogen decomposition by ozonation, experiments were conducted using effluent from an actual wastewater treatment plant. In this experiment, estrogen was added to effluent at a concentration of 200 ng/l and 20 ng/l before the ozonation experiments. The results showed 90% or more of estrogen concentration and estrogenic activity of E2, E1 and EE2 to be removed at an ozone dose of 1 mg/l. At an ozone dose of 3 mg/l, the estrogen concentration and estrogenic activity of E2, E1 and EE2 in the treated water fell below the detection limit. The removal rate was not influenced by the kind of estrogen. No generation of byproducts with estrogenic activity was observed. The authors conclude that estrogen in secondary treated wastewater can be almost entirely removed at the practical ozone dose rate applied for the purpose of disinfection, which is up to about 5 mg/l.

scholarly journals Biological Activity of Wastewater Assessed Using in Vitro Cell-Based Assays

2021 ◽  
Author(s):  
Adamo R. Petosa ◽  
Monica Nowierski ◽  
Viviane Yargeau
Keyword(s):  
Wastewater Treatment ◽  
Wastewater Treatment Plants ◽  
Estrogenic Activity ◽  
Solid Phase ◽  
Treated Wastewater ◽  
Chemical Analyses ◽  
Untreated Wastewater ◽  
17Β Estradiol ◽  
Receiving Waters

Abstract Bioanalytical tools, namely in vitro bioassays, can be employed in tandem with chemical analyses to assess the efficacy of wastewater treatment and the potential for adverse effects from the discharges of wastewater into receiving waters. In the present study, samples of untreated wastewater (i.e. influent) and treated wastewater (i.e. effluent) were collected from two wastewater treatment plants and a wastewater treatment lagoon serving municipalities in southern Ontario, Canada. In addition, grab samples of surface water were collected downstream of the lagoon discharge. After solid phase extraction (SPE) using ion-exchange columns for basic/neutral and acidic compounds, respectively, the extracts were analyzed for a suite of 16 indicator compounds. The two SPE extracts were combined for analysis of biological responses in four in vitro cell-based bioassays. The concentrations of several indicator compounds, including the estrogens, 17β-estradiol and 17α-ethinylestradiol, were below the limits of detection. However, androstenedione and estrone were detected in several influent samples. The concentrations of these steroid hormones and some of the other indicator compounds declined during treatment but acesulfame K, carbamazepine, trimethoprim and DEET persisted in the effluent. The MTS- CellTiter 96® AQueous One Solution Cell Proliferation Assay (MTS) indicated that cell viability was not affected by exposure to the extracts. The Qiagen Nuclear Receptors 10-Pathway Reporter Array indicated that several cellular pathways were upregulated, with the greatest upregulation observed with the estrogen receptor (i.e. induction ratios 12 to 47) and the liver X receptor (i.e. induction ratios 10 to 45). The ERα CALUX assay indicated that estrogenic activity was lower in effluents compared to influents, with the greatest estrogenic activity observed for grab samples of influent from the lagoon (i.e. 56-215 ng L-1 17β-estradiol equivalents). Finally, the results of the Nrf2 Luciferase Luminescence Assay indicated a lower oxidative stress in the effluent samples. Overall, the present study demonstrates that chemical analyses are limited in their ability to predict or explain reductions in the toxicity of treated wastewater. There are thus advantages to using a combination of chemical analyses and in vitro bioassays to monitor the treatment efficiency of wastewater treatment plants and to predict the potential impacts of wastewater discharges into receiving waters.

scholarly journals Sewer Mining as a Distributed Intervention for Water-Energy-Materials in the Circular Economy Suitable for Dense Urban Environments: A Real World Demonstration in the City of Athens

Water ◽  
2021 ◽  
Vol 13 (19) ◽  
pp. 2764
Author(s):  
Argyro Plevri ◽  
Klio Monokrousou ◽  
Christos Makropoulos ◽  
Christos Lioumis ◽  
Nikolaos Tazes ◽  
...  
Keyword(s):  
Wastewater Treatment ◽  
Circular Economy ◽  
Water Reuse ◽  
Wastewater Treatment Plants ◽  
Treatment Plant ◽  
Urban Environments ◽  
Treated Wastewater ◽  
Aquifer Recharge ◽  
Treatment Unit ◽  
Plant Nursery

Water reuse and recycling is gaining momentum as a way to improve the circularity of cities, while recognizing the central role of water within a circular economy (CE) context. However, such interventions often depend on the location of wastewater treatment plants and the treatment technologies installed in their premises, while relying on an expensive piped network to ensure that treated wastewater gets transported from the treatment plant to the point of demand. Thus, the penetration level of treated wastewater as a source of non-potable supply in dense urban environments is limited. This paper focuses on the demonstration of a sewer mining (SM) unit as a source of treated wastewater, as part of a larger and more holistic configuration that examines all three ‘streams’ associated with water in CE: water, energy and materials. The application area is the Athens Plant Nursery, in the (water stressed) city of Athens, Greece. SM technology is in fact a mobile wastewater treatment unit in containers able to extract wastewater from local sewers, treat it directly and reuse at the point of demand even in urban environments with limited space. The unit consists of a membrane bioreactor unit (MBR) and a UV disinfection unit and produces high quality reclaimed water for irrigation and also for aquifer recharge during the winter. Furthermore, a short overview of the integrated nutrient and energy recovery subsystem is presented in order to conceptualise the holistic approach and circularity of the whole configuration. The SM technology demonstrates flexibility, scalability and replicability, which are important characteristics for innovation uptake within the emerging CE context and market.

scholarly journals Isolation and antibiotic profile ofVibriospp. in final effluents of two wastewater treatment plants in the Eastern Cape of South Africa

2018 ◽  
Author(s):  
Olayinka Osuolale ◽  
Anthony Okoh
Keyword(s):  
South Africa ◽  
Wastewater Treatment ◽  
Wastewater Treatment Plants ◽  
Treatment Plant ◽  
Treated Wastewater ◽  
Eastern Cape ◽  
Sources Of Pollution ◽  
Antibiotic Drugs ◽  
Treated Effluents ◽  
Vibrio Spp

ABSTRACTBackgroundPoorly or partially treated wastewater disposed of can contaminate water and even properly treated sewage can have its problems. The highlight of this danger is wastewater treatment plants serving as reservoir for proliferation of antibiotic resistant organisms. We have reported the state of two wastewater treatment in the Eastern Cape of South Africa which discharge poorly and partially treated effluents. Our aims to identify Vibrio spp. and their antibiotic profiles in treated final effluent discharge from wastewater treatment plant.MethodsCulture based approach using the TCBS agar for isolationVibriospp., presumptive isolates were purified and confirmed using PCR. The confirmed isolated were also genotyped to identify the species present. The antibiotic profiling of the confirmed isolates was using the CLSI recommended first line antibiotics for Vibrio.ResultsOut of the 786 presumptive isolates, 374 were confirmed asVibriospp. None of the Vibrio spp. pathotypes were present in the confirmed isolates. Randomized isolates of 100 Vibrio spp. were selected, > 90 % of the isolates were susceptible to Ciprofloxacin, and > 50 – 80 % for Ampicillin, Chloramphenicol, Tetracycline, Cefotaxime, and Trimethoprim-sulfamethoxazole respectively.ConclusionsWe are able to isolate Vibrio spp. from treated effluents but none of their pathotypes were present. The antibiotic agents considered for primary testing which are ciprofloxacin was the most effective of the antibiotic drugs, followed by cefotaxime, tetracycline with less susceptibility. Contamination from discharged effluents from wastewater treatment can lead to spread of spread of disease in this environment. The WWTPs studied are sources of pollution to surface water with environmental and public health.

scholarly journals The biomass, survival, reproductive and biomarker responses of Helix pomatia to soil contaminated with treated and untreated wastewater.

2021 ◽  
Author(s):  
Semase Matseleng ◽  
Ozekeke Ogbeide ◽  
Patricks' Otomo Voua
Keyword(s):  
Wastewater Treatment ◽  
Egg Production ◽  
Wastewater Treatment Plants ◽  
Helix Pomatia ◽  
Treatment Plant ◽  
Treated Wastewater ◽  
Invertebrate Fauna ◽  
Untreated Wastewater ◽  
Industrial Town ◽  
Treatment Facilities

Abstract Wastewater treatment facilities in developing countries like South Africa are major sources of contaminants via effluent into the environment, which could portend high toxicity risks for non-target flora and fauna. To this end, a study was conducted to determine the ecotoxicological responses of selected organism to treated and untreated wastewater from the wastewater treatment plants in an industrial town. The snail Helix pomatia was exposed to OECD artificial soil spiked with untreated or treated wastewater at the following concentrations: 0, 25, 50, 75, 100%. The ecotoxicological responses of Helix pomatia to wastewater were determined by assessing the biomass, survival, reproduction and biomarker responses (Catalase ‒ CAT and Acetylcholinesterase ‒ AChE activities). The overall results showed significant effects on the survival, reproduction and biomass of H. pomatia. Similar results were observed for juvenile emergence. An EC50 of 5.751% for egg production and an EC50 of 6.233% for juvenile emergence were determined in the untreated wastewater. Such indices could not be computed for the treated wastewater, indicating a decreased in toxicity between the untreated and the treated samples. For both the AChE and CAT activities, there was no statistical difference between treated and untreated wastewater treatments. The results from this study highlight the toxic effects of untreated wastewater and indicate that treated wastewater (effluent) released from the wastewater treatment plant in Phuthaditjhaba remains suitable for invertebrate fauna such as H. pomatia.

Phycoremediation of tertiary municipal wastewater: a new insight of filamentous algae coculture in Nordic country

2021 ◽  
Author(s):  
Yan Li ◽  
Ethan Wood ◽  
Gergely Kosa ◽  
Bushra Muzamil ◽  
Christian Vogelsang ◽  
...  
Keyword(s):  
Wastewater Treatment ◽  
Municipal Wastewater ◽  
Wastewater Treatment Plants ◽  
Nordic Country ◽  
Treatment Plant ◽  
Ph Control ◽  
Treated Wastewater ◽  
Filamentous Algae ◽  
Algae Biomass ◽  
Pilot Tests

Abstract This study demonstrated that the application of filamentous co-culture could be a promising supplementary approach to further purify municipal tertiary wastewater in Nordic country. Initial screening of 25 algae strains across multiple genera revealed that Spirogyra sp. and Klebsormidium sp. were suitable for use as a coculture for phycoremediation of the tertiary effluent from a wastewater treatment plant, and this result was validated in three consecutive outdoor pilot tests at 10–15 oC. In the first two batches of pilot tests, the total prosperous and ammonium were depleted close to zero in 24 hours, while the pH in the wastewater increased from 7 to 9. In the 3rd batch, CO2 was thereby added for pH control. Macronutrients (N and P) were successfully removed from the treated wastewater. The total algae biomass increased 2 to 3 times over 7 days with average algae productivity of 1.68 g m2 d− 1. Meanwhile, the produced algae biomass accumulated notable mineral elements (Ca, Mg, K, Fe and Al) and some heavy metals at levels of g kg− 1 and mg kg− 1, respectively. In light of circular economy concept, the produced biomass could be used for different valorizations based on the analytical analysis. This study provides a new insight of phycoremediation for further purification of municipal treated wastewater, by effectively using filamentous algae coculture. Given a great potential for further optimization and improvement, this proof of concept will benefit to the green transition of wastewater treatment plants in Nordic country.

scholarly journals Toxigenic Clostridium difficile PCR Ribotypes from Wastewater Treatment Plants in Southern Switzerland

2012 ◽  
Vol 78 (18) ◽  
pp. 6643-6646 ◽  
Author(s):  
Vincenza Romano ◽  
Vincenzo Pasquale ◽  
Karel Krovacek ◽  
Federica Mauri ◽  
Antonella Demarta ◽  
...  
Keyword(s):  
Wastewater Treatment ◽  
Clostridium Difficile ◽  
Wastewater Treatment Plants ◽  
Treatment Plant ◽  
Treated Wastewater ◽  
Binary Toxin ◽  
Toxin B ◽  
Content Type ◽  
Human Infections ◽  
Reuse Of Treated Wastewater

ABSTRACTThe occurrence ofClostridium difficilein nine wastewater treatment plants in the Ticino Canton (southern Switzerland) was investigated. The samples were collected from raw sewage influents and from treated effluents. Forty-seven out of 55 characterizedC. difficilestrains belonged to 13 different reference PCR ribotypes (009, 010, 014, 015, 039, 052, 053, 066, 070, 078, 101, 106, and 117), whereas 8 strains did not match any of those available in our libraries. The most frequently isolated ribotype (40%) was 078, isolated from six wastewater treatment plants, whereas ribotype 066, a toxigenic emerging ribotype isolated from patients admitted to hospitals in Europe and Switzerland, was isolated from the outgoing effluent of one plant. The majority of the isolates (85%) were toxigenic. Forty-nine percent of them produced toxin A, toxin B, and the binary toxin (toxigenic profile A+B+CDT+), whereas 51% showed the profile A+B+CDT−. Interestingly, eight ribotypes (010, 014, 015, 039, 066, 078, 101, and 106) were among the riboprofiles isolated from symptomatic patients admitted to the hospitals of the Ticino Canton in 2010. Despite the limitation of sampling, this study highlights that toxigenic ribotypes ofC. difficileinvolved in human infections may occur in both incoming and outgoing biological wastewater treatment plants. Such a finding raises concern about the possible contamination of water bodies that receive wastewater treatment plant effluents and about the safe reuse of treated wastewater.

scholarly journals Evaluation of the Presence of Endocrine-Disrupting Compounds in Dissolved and Solid Wastewater Treatment Plant Samples of Gran Canaria Island (Spain)

2013 ◽  
Vol 2013 ◽  
pp. 1-15 ◽  
Author(s):  
T. Vega-Morales ◽  
Z. Sosa-Ferrera ◽  
J. J. Santana-Rodríguez
Keyword(s):  
Wastewater Treatment ◽  
Wastewater Treatment Plants ◽  
Estrogenic Activity ◽  
Solid Phase ◽  
Treatment Plant ◽  
Endocrine Disrupting Compounds ◽  
Gran Canaria ◽  
Assisted Extraction ◽  
Endocrine Disrupting ◽  
Ultrasonic Assisted

Liquid and solid samples from two wastewater treatment plants (WWTPs) on Gran Canaria Island (Spain) have been tested for the presence of compounds with endocrine-disrupting properties. The selected degradation stages were sampled bimonthly from each WWTP over the 12-month period from July 2010 to July 2011. The analytical methods used for the determination of the endocrine-disrupting compounds (EDCs) were based on on-line solid phase extraction, microwave-assisted extraction (MAE), and ultrasonic-assisted extraction (UAE) coupled to UHPLC-MS/MS. All of the hyphenated methodologies employed in this work showed good recoveries (72–104%) and sensitivities, with LODs lower than 7.0 ng L−1and 6.3 ng g−1for the dissolved and solid fractions, respectively. We have also evaluated the estrogenicity of the samples in terms of their estradiol equivalent concentrations (EEQs). The chemical analysis of the selected EDCs revealed fairly low concentrations for both natural and synthetic oestrogens, alkylphenolic compounds, and bisphenol-A in each of the dissolved, particulate, and sludge samples (ng L−1or ng g−1). However, the estimated estrogenic activity indicated that the majority of samples could represent an important environmental risk, clearly surpassing the threshold to exert deleterious consequences on living beings.

scholarly journals ENVIRONMENTAL CONSIDERATIONS OF LARGE WASTEWATER TREATMENT PLANTS – THE CITY OF NIŠ CASE STUDY

2019 ◽  
pp. 015
Author(s):  
Dejan Vasović ◽  
Sandra Stanković ◽  
Ljiljana Takić
Keyword(s):  
Wastewater Treatment ◽  
Water Supply ◽  
Wastewater Treatment Plants ◽  
Treatment Plant ◽  
Special Problem ◽  
Public Utility ◽  
Treated Wastewater ◽  
The Republic ◽  
The City ◽  
Wastewater Collection

Nowadays, the Republic of Serbia is at the bottom of the scale of European countries in terms of public utility equipment of the respective communal infrastructure particularly within the area of wastewater collection and treatment. The current situation in the area of water resources management in the Republic of Serbia indicates that the basic problems are insufficient construction of sewerage infrastructure in cities, i.e. insufficient coverage with the sewerage network of users connected to either public or private water supply network, followed by an insufficient number of constructed and operative wastewater treatment plants. In this sense, a particular problem is a requirement for relatively large initial investments in wastewater collection and treatment sector. Also, a special difficulty is the low price of delivered and channeled water within the existing system of public utilities, which is insufficient to provide maintenance of the existing water supply and sewage systems, regardless the future development and improvement of the existing system. Currently, only 10% of total produced wastewater quantity is treated in the Republic of Serbia, with different procedures and with uneven and, most often, insufficient purification effects. In addition to wastewater generated by the population (i.e. households connected to the sewer system), the special problem is the inadequate treatment of industrial wastewater. On the other hand, disposition of treated wastewater, disposal of separated sludge from the treatment process, odor and pest control, noise control, working conditions, etc. are significant challenges for the scientists, professionals and operators. Therefore, the aim of this paper is to comprehensively examine all environmental aspects on the example of a large wastewater treatment plant which is to be gained by the city of Niš, in the very near future.

scholarly journals Pollution Characteristics and Emissions of Typical Organophosphate Esters From a Wastewater Treatment Plant in Chengdu, China

2021 ◽  
Author(s):  
Hongling Yin ◽  
Yi Luo ◽  
Jiaojiao Song ◽  
Shiping Li ◽  
Shuyu Lin ◽  
...  
Keyword(s):  
Wastewater Treatment ◽  
Southwest China ◽  
Wastewater Treatment Plants ◽  
Removal Rate ◽  
Treatment Plant ◽  
Total Removal ◽  
Organophosphate Esters ◽  
Rainy Period ◽  
Influent Water ◽  
Daily Discharge

Abstract Effluent from wastewater treatment plants (WWTP) is an important source of organophosphate esters (OPEs) in the receiving rivers. In this paper, the concentration and distribution of seven OPEs in the water samples were determined, and the discharge was estimated. The results showed that the total removal rate of Σ7OPEs in water phase in WWTP was 57.2%. The average concentrations of Σ7OPEs in influent and effluent of the WWTP during rainy period were 3956.1 ± 1897.3 ng/L and 1461.9 ± 846.3 ng/L, respectively, which were about 4 times larger than those in influent water (978.2 ± 166.5 ng/L) and effluent (418.3 ± 12.0 ng/L) during non-rainy period, indicating that rainfall has a marked impact on the load of OPEs in WWTP and the receiving water. It was estimated that the average daily discharge of Σ7OPEs in the effluent of WWTP was 157.9 g, and the daily per capita contribution of the population in the area to the OPEs in the influent was 0.414 mg. During the shift of labour-intensive manufacturing from the coastal developed areas to inland regions, OPEs were widely used and produced in Southwest China. The total amount of OPEs emissions and its control should be taken into consideration.

scholarly journals An Ohio State Scenic River Shows Elevated Antibiotic Resistance Genes, Including Acinetobacter Tetracycline and Macrolide Resistance, Downstream of Wastewater Treatment Plant Effluent

2021 ◽  
Author(s):  
April Murphy ◽  
Daniel Barich ◽  
M. Siobhan Fennessy ◽  
Joan L. Slonczewski
Keyword(s):  
Antibiotic Resistance ◽  
Wastewater Treatment ◽  
Resistance Genes ◽  
Wastewater Treatment Plants ◽  
Treatment Plant ◽  
Antibiotic Resistance Genes ◽  
Treated Wastewater ◽  
Flow Volume ◽  
Recreational Use ◽  
Wastewater Treatment Plant Effluent

Antibiotic resistance is a growing problem worldwide, with frequent transmission between pathogens and environmental organisms. Rural rivers can support high levels of recreational use by people unaware of inputs from treated wastewater, while wastewater treatment plants (WWTPs) can generate a small but significant portion of flow volume into a river surrounded by forest and agriculture.

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