scholarly journals Evaluation of the Presence of Endocrine-Disrupting Compounds in Dissolved and Solid Wastewater Treatment Plant Samples of Gran Canaria Island (Spain)

2013 ◽  
Vol 2013 ◽  
pp. 1-15 ◽  
Author(s):  
T. Vega-Morales ◽  
Z. Sosa-Ferrera ◽  
J. J. Santana-Rodríguez
Keyword(s):  
Wastewater Treatment ◽  
Wastewater Treatment Plants ◽  
Estrogenic Activity ◽  
Solid Phase ◽  
Treatment Plant ◽  
Endocrine Disrupting Compounds ◽  
Gran Canaria ◽  
Assisted Extraction ◽  
Endocrine Disrupting ◽  
Ultrasonic Assisted

Liquid and solid samples from two wastewater treatment plants (WWTPs) on Gran Canaria Island (Spain) have been tested for the presence of compounds with endocrine-disrupting properties. The selected degradation stages were sampled bimonthly from each WWTP over the 12-month period from July 2010 to July 2011. The analytical methods used for the determination of the endocrine-disrupting compounds (EDCs) were based on on-line solid phase extraction, microwave-assisted extraction (MAE), and ultrasonic-assisted extraction (UAE) coupled to UHPLC-MS/MS. All of the hyphenated methodologies employed in this work showed good recoveries (72–104%) and sensitivities, with LODs lower than 7.0 ng L−1and 6.3 ng g−1for the dissolved and solid fractions, respectively. We have also evaluated the estrogenicity of the samples in terms of their estradiol equivalent concentrations (EEQs). The chemical analysis of the selected EDCs revealed fairly low concentrations for both natural and synthetic oestrogens, alkylphenolic compounds, and bisphenol-A in each of the dissolved, particulate, and sludge samples (ng L−1or ng g−1). However, the estimated estrogenic activity indicated that the majority of samples could represent an important environmental risk, clearly surpassing the threshold to exert deleterious consequences on living beings.

Detection and removal of estrogencity in membrane biological reactors

2006 ◽  
Vol 6 (6) ◽  
pp. 19-26 ◽  
Author(s):  
J.Y. Hu ◽  
X. Chen
Keyword(s):  
Estrogenic Activity ◽  
Solid Phase ◽  
Treatment Plant ◽  
Endocrine Disrupting Compounds ◽  
Pilot Scale ◽  
Membrane Bioreactors ◽  
Yeast Cells ◽  
Endocrine Disrupting ◽  
And Behavior ◽  
Fate And Behavior

Three pilot-scale submerged membrane bioreactors (MBRs) in a local wastewater treatment plant (K, M and Z) were studied with the objective to compare the performance of pre-denitrification MBR systems in eliminating the estrogenic activity of the effluent of primary clarifier. A total of 5 batches of samples, which included influent, effluent, supernatant and sludge from the respective aerobic and anoxic tanks were collected over the span. They were investigated by using the developed solid-phase extraction (SPE) protocol coupled with a modified yeast-based estrogen screen (YES) assay. From the results, it could be seen that M MBR demonstrated the best endocrine disrupting compounds (EDCs) removal efficiency. The fate and behavior of EDCs in MBR systems were fairly understood with estrogenic activity formation dominating in the anoxic tank and removal dominating in the aerobic tank. It is believed that the sorption of EDCs onto the sludge as well as biodegradation of EDCs might be the key mechanisms for the EDCs removal. The low response of YES when dealing with influent samples was mainly due to the inhibition and antagonist effects induced by the influent samples on yeast cells.

scholarly journals Occurrence and Risk Assessment of Steroidal Hormones and Phenolic Endocrine Disrupting Compounds in Surface Water in Cuautla River, Mexico

Water ◽  
2019 ◽  
Vol 11 (12) ◽  
pp. 2628 ◽  
Author(s):  
Griselda Marissa Calderón-Moreno ◽  
Josefina Vergara-Sánchez ◽  
Hugo Saldarriaga-Noreña ◽  
Maria Luisa García-Betancourt ◽  
Martha Lilia Domínguez-Patiño ◽  
...  
Keyword(s):  
Surface Water ◽  
Estrogenic Activity ◽  
Solid Phase ◽  
Treatment Plant ◽  
Domestic Wastewater ◽  
Aquatic Organisms ◽  
Endocrine Disrupting Compounds ◽  
Potential Ecological Risk ◽  
Endocrine Disrupting ◽  
Steroidal Hormones

In this study, two hormones 17β-estradiol (E2) and 17α-ethynylestradiol (EE2), and three phenolic compounds, bisphenol A (BPA), 4-N-nonylphenol (4-NP) and 4-tert-octylphenol (4-t-OP), were determined in surface water in the Cuautla River at the State of Morelos during the dry-season in Mexico. The endocrine disrupting compounds (EDCs) were extracted from water samples using solid-phase extraction (SPE) with end-capped C18, and then the extracts were chemically derivatized to TMS (trimethylsylane)-compounds and analyzed by gas chromatography coupled to mass spectrometry (GCMS). The most abundant compound was BPA (22.46 ± 30.17 ng L−1), followed by 4-t-OP (11.24 ± 11.76 ng L−1), 4-NP (7.53 ± 14.88 ng L−1), EE2 (2.37 ± 4.36 ng L−1) and E2 (0.97 ± 1.82 ng L−1). The residual amounts of target compounds could either reach stream surface water from direct domestic wastewater discharges, conventional wastewater treatment plant or can be a result from the use of agrochemicals in crop areas. The EDCs in Cuautla River exerted a high pressure on the aquatic ecosystem because their presences in surface water caused medium and high potential ecological risk. Besides, it was found that aquatic organisms were exposed to estrogenic activity.

Nanofiltration of endocrine disrupting compounds

2003 ◽  
Vol 3 (5-6) ◽  
pp. 321-327 ◽  
Author(s):  
M. Gallenkemper ◽  
T. Wintgens ◽  
T. Melin
Keyword(s):  
Wastewater Treatment ◽  
Municipal Wastewater ◽  
Wastewater Treatment Plants ◽  
Endocrine Disrupting Compounds ◽  
Municipal Wastewater Treatment ◽  
Water And Wastewater Treatment ◽  
Wide Range ◽  
Endocrine Disrupting ◽  
Municipal Wastewater Treatment Plants ◽  
Set Up

Endocrine disrupting compounds can affect the hormone system in organisms. A wide range of endocrine disrupters were found in sewage and effluents of municipal wastewater treatment plants. Toxicological evaluations indicate that conventional wastewater treatment plants are not able to remove these substances sufficiently before disposing effluent into the environment. Membrane technology, which is proving to be an effective barrier to these substances, is the subject of this research. Nanofiltration provides high quality permeates in water and wastewater treatment. Eleven different nanofiltration membranes were tested in the laboratory set-up. The observed retention for nonylphenol (NP) and bisphenol A (BPA) ranged between 70% and 100%. The contact angle is an indicator for the hydrophobicity of a membrane, whose influence on the permeability and retention of NP was evident. The retention of BPA was found to be inversely proportional to the membrane permeability.

scholarly journals Biological Activity of Wastewater Assessed Using in Vitro Cell-Based Assays

2021 ◽  
Author(s):  
Adamo R. Petosa ◽  
Monica Nowierski ◽  
Viviane Yargeau
Keyword(s):  
Wastewater Treatment ◽  
Wastewater Treatment Plants ◽  
Estrogenic Activity ◽  
Solid Phase ◽  
Treated Wastewater ◽  
Chemical Analyses ◽  
Untreated Wastewater ◽  
17Β Estradiol ◽  
Receiving Waters

Abstract Bioanalytical tools, namely in vitro bioassays, can be employed in tandem with chemical analyses to assess the efficacy of wastewater treatment and the potential for adverse effects from the discharges of wastewater into receiving waters. In the present study, samples of untreated wastewater (i.e. influent) and treated wastewater (i.e. effluent) were collected from two wastewater treatment plants and a wastewater treatment lagoon serving municipalities in southern Ontario, Canada. In addition, grab samples of surface water were collected downstream of the lagoon discharge. After solid phase extraction (SPE) using ion-exchange columns for basic/neutral and acidic compounds, respectively, the extracts were analyzed for a suite of 16 indicator compounds. The two SPE extracts were combined for analysis of biological responses in four in vitro cell-based bioassays. The concentrations of several indicator compounds, including the estrogens, 17β-estradiol and 17α-ethinylestradiol, were below the limits of detection. However, androstenedione and estrone were detected in several influent samples. The concentrations of these steroid hormones and some of the other indicator compounds declined during treatment but acesulfame K, carbamazepine, trimethoprim and DEET persisted in the effluent. The MTS- CellTiter 96® AQueous One Solution Cell Proliferation Assay (MTS) indicated that cell viability was not affected by exposure to the extracts. The Qiagen Nuclear Receptors 10-Pathway Reporter Array indicated that several cellular pathways were upregulated, with the greatest upregulation observed with the estrogen receptor (i.e. induction ratios 12 to 47) and the liver X receptor (i.e. induction ratios 10 to 45). The ERα CALUX assay indicated that estrogenic activity was lower in effluents compared to influents, with the greatest estrogenic activity observed for grab samples of influent from the lagoon (i.e. 56-215 ng L-1 17β-estradiol equivalents). Finally, the results of the Nrf2 Luciferase Luminescence Assay indicated a lower oxidative stress in the effluent samples. Overall, the present study demonstrates that chemical analyses are limited in their ability to predict or explain reductions in the toxicity of treated wastewater. There are thus advantages to using a combination of chemical analyses and in vitro bioassays to monitor the treatment efficiency of wastewater treatment plants and to predict the potential impacts of wastewater discharges into receiving waters.

Characteristics of estrogen decomposition by ozonation

2006 ◽  
Vol 54 (10) ◽  
pp. 87-93 ◽  
Author(s):  
T. Hashimoto ◽  
K. Takahashi ◽  
T. Murakami
Keyword(s):  
Wastewater Treatment ◽  
Wastewater Treatment Plants ◽  
Estrogenic Activity ◽  
Removal Rate ◽  
Treatment Plant ◽  
Treated Wastewater ◽  
Ozone Dose ◽  
Natural Estrogens ◽  
Ethynyl Estradiol ◽  
Estrogen Concentration

Since the natural estrogens 17 β-estradiol (E2) and estron (E1), and the synthetic estrogen 17 α-ethynyl estradiol (EE2) have strong endocrine disrupting effects and the tendency to persist in effluent from wastewater treatment plants, effective measures are needed to remove them from wastewater. In this research, to gain an understanding of the characteristics of estrogen decomposition by ozonation, experiments were conducted using effluent from an actual wastewater treatment plant. In this experiment, estrogen was added to effluent at a concentration of 200 ng/l and 20 ng/l before the ozonation experiments. The results showed 90% or more of estrogen concentration and estrogenic activity of E2, E1 and EE2 to be removed at an ozone dose of 1 mg/l. At an ozone dose of 3 mg/l, the estrogen concentration and estrogenic activity of E2, E1 and EE2 in the treated water fell below the detection limit. The removal rate was not influenced by the kind of estrogen. No generation of byproducts with estrogenic activity was observed. The authors conclude that estrogen in secondary treated wastewater can be almost entirely removed at the practical ozone dose rate applied for the purpose of disinfection, which is up to about 5 mg/l.

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