scholarly journals Anaerobic degradation of phenol in wastewater at ambient temperature

2004 ◽  
Vol 49 (1) ◽  
pp. 95-102 ◽  
Author(s):  
H.H.P. Fang ◽  
Y. Liu ◽  
S.Z. Ke ◽  
T. Zhang
Keyword(s):  
Ambient Temperature ◽  
Retention Time ◽  
Anaerobic Degradation ◽  
Hydraulic Retention Time ◽  
Phenol Degradation ◽  
Upflow Anaerobic Sludge Blanket ◽  
Synthetic Wastewater ◽  
Anaerobic Sludge ◽  
Anaerobic Sludge Blanket

Treating a synthetic wastewater containing phenol as the sole substrate at 26°C, an upflow anaerobic sludge blanket reactor was able to remove over 98% of phenol up to 1,260 mg/l in wastewater with 12 h of hydraulic retention time, corresponding to 6.0 g-COD/(l·day). Results showed that benzoate was the key intermediate of phenol degradation. Conversion of benzoate to methane was suppressed by the presence of phenol. Based on DNA cloning analysis, the sludge was composed of five groups of microorganisms. Desulfotomaculum and Clostridium were likely responsible for the conversion of phenol to benzoate, which was further degraded by Syntrophus to acetate and H2/CO2. Methanogens lastly converted acetate and H2/CO2 to methane. The role of epsilon-Proteobacteria was, however, unclear.

Degradation of 3-Nitrophenol with Sodium Acetate as Co-Substrate in an Upflow Anaerobic Sludge Blanket Reactor

2011 ◽  
Vol 393-395 ◽  
pp. 1153-1156
Author(s):  
Zong Lian She ◽  
Lei Lei Li ◽  
Ying Jie Zhu ◽  
Tian Xie ◽  
Li Na Jiang ◽  
...  
Keyword(s):  
Treatment Effect ◽  
Retention Time ◽  
Conversion Rate ◽  
Sodium Acetate ◽  
Hydraulic Retention Time ◽  
Operating Temperature ◽  
Upflow Anaerobic Sludge Blanket ◽  
Uasb Reactor ◽  
Anaerobic Sludge ◽  
Anaerobic Sludge Blanket

The effects on degrading 3-nitrophenol (3-NP) with sodium acetate as co-substrate under the conditions of laboratory-scale upflow anaerobic sludge blanket (UASB) reactor was investigated at an operating temperature of 35±1°C in this study. The results showed that the optimum influent 3-NP concentration was 71.6mg/L when keeping influent COD concentration for 2500 mg/L and hydraulic retention time (HRT) for 30 h. At the stage of reducing influent COD concentration, all of the 3-NP removal rates were more than 95%; while the conversion rate of 3-aminophenol (3-AP) decreased from 61.4% to 0.2%. Meanwhile, the reduction of HRT also had significantly effects on the treatment effect of 3-NP. With the decreases of HRT, COD removal dropped to 49.7% from 82.7%.

scholarly journals Degradation of phenol and p-cresol in reactors

2000 ◽  
Vol 42 (5-6) ◽  
pp. 237-244 ◽  
Author(s):  
Herbert H.P. Fang ◽  
Gong-Ming Zhou
Keyword(s):  
Removal Efficiency ◽  
Retention Time ◽  
Hydraulic Retention Time ◽  
Phenol Concentration ◽  
Upflow Anaerobic Sludge Blanket ◽  
Anaerobic Sludge ◽  
Effluent Recirculation ◽  
Anaerobic Sludge Blanket

The effects of hydraulic retention time (HRT) and phenol concentration on the degradation of phenol and p-cresol in wastewater were investigated in two respective UASB (upflow anaerobic sludge blanket) reactors with effluent recirculation at 37 °C for over 440 days. After acclimation, nearly all the phenol and p-cresol at moderate concentrations could be degraded without carbohydrate as a co-substrate. Treating a wastewater containing 800 mg/l of phenol and 300 mg/l of p-cresol at HRT ranging 2–12 hours, the first reactor consistently removed 95% of phenol, 65% of p-cresol and 85% of COD at 8–12 hours of HRT; the efficiency, however, decreased at lower HRT. Treating wastewater containing a constant p-cresol concentration of 400 mg/l at 24 hours of HRT, the second reactor was able to remove 75–80% of COD when the phenol was 1200 and 1500 mg/l; the removal efficiency decreased as phenol concentration further increased. High levels of residual phenol and p-cresol in the effluent suppressed the activity of biogranules. The suppression of bioactivity was not permanent. Biomass was able to regain its activity fully after lowering the phenolic concentrations in the wastewater.

scholarly journals Hydrodynamic behavior of a lab-scale upflow anaerobic sludge blanket reactor (UASB) operated with an adopted hydraulic retention time (HRT) of 12 hours

2009 ◽  
Vol 33 (4) ◽  
pp. 1139-1144 ◽  
Author(s):  
Aguinaldo Menegassi Pereira Lourenço ◽  
Cláudio Milton Montenegro Campos
Keyword(s):  
Retention Time ◽  
Hydraulic Retention Time ◽  
Upflow Anaerobic Sludge Blanket ◽  
Uasb Reactor ◽  
Anaerobic Sludge ◽  
Physical Analysis ◽  
Hydrodynamic Behavior ◽  
Operational Conditions ◽  
Hydrodynamic Parameters ◽  
Anaerobic Sludge Blanket

The present research was carried out in the Laboratory of Water Analysis at the Engineering Department at Federal University of Lavras (LWAED-UFLA), in order to evaluate the hydrodynamic behavior of a lab-scale upflow anaerobic sludge blanket reactor (UASB) that was continuously fed with liquid effluent from swine manure with solid separation over 2mm. The hydrodynamic parameters were determined by a tracer study, under hydraulic retention time (HRT) of 12 hours, using Lithium Chloride (LiCl) as a tracer. The system was monitored periodically through physical analysis of samples collected at UASB, during the steady-state operational conditions. The physical-chemical analyses were accomplished using a flame photometry. The operational average temperature in the UASB reactor was 23.9ºC .The UASB hydrodynamic parameters determined were: average residence time (<img src="/img/revistas/cagro/v33n4/t4_barra.gif" align="absmiddle">) of 38.3 h, number of dispersion d= 0.27, and the flow type was characterized as dispersed flow of great intensity. This research is of great importance due to the fact that the scaling-up of biological reactors is based on the hydrodynamic behavior, through which the bacterial kinetic is directly influenced, as reported by Saleh (2004).

Alternative biological systems for the treatment of vinasse from wine

2010 ◽  
Vol 62 (12) ◽  
pp. 2899-2904 ◽  
Author(s):  
A. Vlyssides ◽  
E. M. Barampouti ◽  
S. Mai ◽  
A. Stamatoglou ◽  
E. Tsimas
Keyword(s):  
Retention Time ◽  
Hydraulic Retention Time ◽  
Upflow Anaerobic Sludge Blanket ◽  
Uasb Reactor ◽  
Anaerobic Sludge ◽  
Environmental Technology ◽  
Ferrous Sulfide ◽  
System 2 ◽  
Anaerobic Sludge Blanket ◽  
System 1

This work studied alternative treatment schemes for the vinasse wastewater from wine distilleries aiming at overcoming the problems caused by the high nitrogen and sulfur concentrations. A plexiglas laboratory-scale upflow anaerobic sludge blanket (UASB) reactor of 20 L volume that was operated at 45°C and hydraulic retention time 1 d, was included in all the examined systems. System 1 was the conventional UASB reactor, system 2 was the UASB reactor supplemented with iron. System 3 consisted of the UASB reactor supplemented with iron and a CSTR reactor that operated under the following conditions: Diluted Oxygen 1.2 mg/L, Hydraulic Retention Time 1 d, pH 6.7 and Temperature 45°C. System 3 aimed at converting ammonium directly to dinitrogen gas under anaerobic conditions but it needed to be preceeded by a first partial nitrification step. All systems had high COD efficiencies over 75%. Ferrous iron addition apart from enhancing the performance of systems 2 and 3, it was able to retain all sulphur content of the wastewater as ferrous sulfide stripping the biogas from hydrogen sulfide. System 3 also managed to meet its goal, since it achieved an 86% nitrogen reduction. Conclusively, system 3 seems to be a very promising environmental technology for the treatment of distillery and winery byproducts, as well as industrial wastewater with high sulfur and nitrogen content.

Acclimation of Anaerobic Granular Sludge and 2,6-DNP Removal in the Upflow Anaerobic Sludge Blanket (UASB) Reactors

2011 ◽  
Vol 183-185 ◽  
pp. 352-361
Author(s):  
Zong Lian She ◽  
En Shi ◽  
Xiao Hui Fu ◽  
Jian Wu ◽  
Li Na Jiang ◽  
...  
Keyword(s):  
Anaerobic Degradation ◽  
Hydraulic Retention Time ◽  
Granular Sludge ◽  
Upflow Anaerobic Sludge Blanket ◽  
Anaerobic Sludge ◽  
Substrate Removal ◽  
Before And After ◽  
Removal Efficiencies ◽  
Anaerobic Sludge Blanket ◽  
Uasb Reactors

Characteristics of anaerobic granules before and after acclimation were studied using glucose as co-substrate. Removal efficiencies of 2,6-dinitrophenol (2,6-DNP) using two different co-substrates were investigated in two lab-scale UASB reactors. Granular sludge acclimatized to the wastewater containing 2,6-DNP through 3 months. After acclimation, SEM pictures of the granular biomass showed that Filamentous bacteria were the predominant bacteria on the surface of granules. Throughout the study of 2,6-DNP anaerobic degradation with different co-substrates, influent COD concentration was kept constant as about 2500 mg l-1. Maximum 2,6-DNP concentration was 170.0 mg l-1 and 2,6-DNP removal efficiencies were always more than 98.0% using glucose as co-substrate, keeping hydraulic retention time (HRT) as 35 h. When using sodium acetate as co-substrate and keeping HRT as 30 h, maximum 2,6-DNP concentration was up to 189.5 mg l-1 and over 99.2% 2,6-DNP removal efficiencies could be obtained.

scholarly journals Effect of Hydraulic Retention Time on the Treatment of Real Cattle Slaughterhouse Wastewater and Biogas Production from HUASB Reactor

Water ◽  
2020 ◽  
Vol 12 (2) ◽  
pp. 490
Author(s):  
Mohammed Ali Musa ◽  
Syazwani Idrus
Keyword(s):  
Retention Time ◽  
Suspended Solids ◽  
Hydraulic Retention Time ◽  
Biogas Production ◽  
Nitrogen Concentration ◽  
Upflow Anaerobic Sludge Blanket ◽  
Synthetic Wastewater ◽  
Growth Surface ◽  
Anaerobic Sludge ◽  
Slaughterhouse Wastewater

Anaerobic digestion technology provides an alternative route for sustainable management of organic waste. In this study, the performance of the hybrid upflow anaerobic sludge blanket (HUASB) reactor consisting of synthetic grass media as attached growth surface was investigated for the treatment of cattle slaughterhouse wastewater under mesophilic (35 ± 1 °C) condition. After acclimatization with synthetic wastewater, the reactor was loaded up to OLR 10 g L−1d−1, corresponding to 20 g COD/L at a varying hydraulic retention time (HRT) of 24, 30, 36, 42, and 48 h. The system attained a maximum COD removal efficiency of 97% total suspended solids (TSS), volatile suspended solids (VSS), fats, oil, and grease (FOG), color removal, and turbidity were found as 97%, 284 mg/L, 79%, 78%, and 91% respectively. The biogas production after 48 h was found as 38 L/d, with about 85% methane and specific methane production of 0.24 LCH4/gCODadded. The ratio of alkalinity was 0.22, while ammonia nitrogen concentration reached a maximum of 839 mg/L at a steady state. Scanning electron microscopic (SEM) analysis revealed a predominance of Methanosarcina bacteria with the coccoidal shape at the end of the performance study. Therefore, the results of the experiment showed that increasing HRT significantly affects the performance of the system.

scholarly journals PENGOLAHAN LIMBAH ORGANIK DENGAN UPFLOW ANAEROBIC SLUDGE BLANKET (UASB) DI INDUSTRI KECAP

2016 ◽  
Vol 7 (2) ◽  
pp. 81-88
Author(s):  
Bekti Marlena ◽  
Cholid Syahroni ◽  
Sartamtomo Sartamtomo ◽  
Nur Zen
Keyword(s):  
Stainless Steel ◽  
Retention Time ◽  
Hydraulic Retention Time ◽  
Upflow Anaerobic Sludge Blanket ◽  
Anaerobic Sludge ◽  
Anaerobic Sludge Blanket

Telah dilakukan penelitian pengolahan limbah industri kecap dengan menggunakan UASB. Dua reaktor UASB yang terbuat dari stainless steel dengan volume masing-masing 1,165 m3 disusun secara seri. Air limbah yang diolah merupakan buangan dari proses produksi kecap dengan nilai COD dari 2.709 sampai 21.684 mg/L. Uji coba dilakukan dengan kecepatan alir 2,9 m3/hari dan 0,7 m3/hari dengan waktu tinggal (Hydraulic Retention Time) masing-masing 19 jam dan 80 jam. Hasil penelitian menunjukkan bahwa pada percobaan dengan waktu tinggal 19 jam dan kecepatan beban COD rata-rata 11.114 kg/hari, dapat menurunkan nilai COD hingga 70% dengan penurunan beban COD rata-rata adalah 2.842 kg/m3 hari. Percobaan dengan waktu tinggal 80 jam dengan kecepatan beban COD rata-rata adalah 2.837 kg/hari diperoleh hasil penurunan COD hingga 78% dan penurunan beban COD rata-rata adalah 1.335 kg/m3hari, sedangkan untuk kecepatan beban COD rata-rata 2.593 kg/hari diperoleh hasil berturut-turut 76% dan 1.298 kg/m3 hari. Jika dibandingkan dengan kinerja sistem anaerobik yang telah dimiliki oleh industri, maka reaktor UASB hasil penelitian memiliki keunggulan ditinjau dari waktu tinggal dan penurunan beban COD, meskipun persen penurunan COD masih lebih rendah.  

Microbial characteristics of a methanogenic phenol-degrading sludge

2005 ◽  
Vol 52 (1-2) ◽  
pp. 73-78 ◽  
Author(s):  
T. Zhang ◽  
S. Z. Ke ◽  
Y. Liu ◽  
H.P. Fang
Keyword(s):  
Dna Analysis ◽  
Pcr Amplification ◽  
Upflow Anaerobic Sludge Blanket ◽  
Anaerobic Sludge ◽  
Chain Reaction ◽  
Microbial Characteristics ◽  
Polymerase Chain ◽  
Anaerobic Sludge Blanket

Microbial properties of a methanogenic granular phenol-degrading sludge were characterized using the 16S rRNA/DNA-based techniques, including polymerase chain reaction (PCR) amplification, cloning, DNA sequencing, and fluorescence in situ hybridization (FISH). The sludge was sampled from an upflow anaerobic sludge blanket reactor, which removed 98% of phenol (up to 1260 mg/l) in wastewater at 26°C with 12 hours of hydraulic retention. Based on DNA analysis, the Eubacteria in the sludge was composed of 13 operational taxonomy units (OTUs). Two OTUs, one resembling Clostridium and the other remotely resembling Desulfotomaculum, were likely responsible for the conversion of phenol to benzoate, which was further degraded by five Syntrophus-resembling OTUs to acetate and H2/CO2; methanogens lastly converted acetate and H2/CO2 into methane. The role of six remaining OTUs remains unclear. Overall, the sludge was composed of 26±6% Eubacteria and 74±9% methanogens, of which 54±6% were acetotrophic Methanosaetaceae, 14±3% and 3±2% were hydrogenotrophic Methanomicrobiales and Methanobacteriaceae, respectively.

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