Removal of enteric viruses and selected microbial indicators by UV irradiation of secondary effluent

2003 ◽  
Vol 47 (9) ◽  
pp. 193-198 ◽  
Author(s):  
J.G. Jacangelo ◽  
P. Loughran ◽  
B. Petrik ◽  
D. Simpson ◽  
C. McIlroy
Keyword(s):  
Wastewater Treatment ◽  
New Zealand ◽  
Clostridium Perfringens ◽  
Uv Irradiation ◽  
Secondary Effluent ◽  
Sufficient Information ◽  
Indicator Organisms ◽  
Microbial Indicators ◽  
Uv Dose ◽  
Specific Bacteriophage

The Watercare Mangere Wastewater Treatment Facility, which treats wastewater from the greater Auckland New Zealand region, is undergoing a major expansion/upgrading to add advanced treatment and disinfection prior to discharge into a harbor. One important goal of this project is to protect the receiving water from microbial contamination. Since sufficient information on the fate of various microorganisms through wastewater treatment plants in New Zealand was not readily available, extensive pilot- and bench-scale studies were undertaken to develop specific design criteria for the treatment and disinfection systems. The specific objective of this study was to evaluate the removal and inactivation of enteric pathogens and other microbial indicators through treatment processes that employs UV irradiation as a final disinfection process. The removal of indicator organisms through secondary treatment was typically between 2.5-log (99.7% removal) and 2.8-log (99.8% removal) for fecal coliforms and enterococci, respectively. Indigenous F-specific bacteriophage exhibited a mean removal of 1.6-log (i.e. 97.7% removal) and Clostridium perfringens spores showed a mean removal of 1.3-log (i.e. 95% removal). The UV dose required to achieve a one log reduction in the concentration of indigenous F-specific bacteriophage was found to be approximately 20 mWs/cm2 per log removal. The concentration of enterovirus and adenovirus were consistently reduced to the limit of detection (1 TCID50/100L) at UV doses of 35 to 40 mWs/cm2 and 40 to 45 mWs/cm2, respectively. Clostridium perfringens spores were the most resistant indicator organisms, being reduced to less than 200 MPN/100 mL at a UV dose of 75 mWs/cm2.

Comparison of F-specific bacteriophage, enterococci, and faecal coliform densities through a wastewater treatment process employing oxidation ponds

1995 ◽  
Vol 31 (5-6) ◽  
pp. 85-89 ◽  
Author(s):  
S. J. Turner ◽  
G. D. Lewis
Keyword(s):  
Wastewater Treatment ◽  
Treatment Process ◽  
Faecal Coliform ◽  
Indicator Organisms ◽  
Faecal Coliforms ◽  
Wastewater Treatment Process ◽  
Oxidation Pond ◽  
Oxidation Ponds ◽  
Specific Bacteriophage

Over a 12 month period F-specific bacteriophages, faecal coliforms and enterococci were compared as microbial indicator organisms for the quality of a wastewater treatment (oxidation pond) system. Results suggest that enterococci may be the most useful indicator for oxidation pond systems.

UV disinfection of wastewater effluents for unrestricted irrigation

2006 ◽  
Vol 54 (3) ◽  
pp. 83-88 ◽  
Author(s):  
A.M. Nasser ◽  
H. Paulman ◽  
O. Sela ◽  
T. Ktaitzer ◽  
H. Cikurel ◽  
...  
Keyword(s):  
Uv Irradiation ◽  
Wastewater Reuse ◽  
High Rate ◽  
Uv Disinfection ◽  
Secondary Effluent ◽  
E Coli ◽  
Uv Dose ◽  
Inactivation Efficiency ◽  
Transmission Of Disease

Wastewater reuse in arid regions is important for the production of a water resource to be utilised for non-potable purposes and to prevent the environmental transmission of disease-causing agents. This study was conducted to evaluate the effect of water quality on the comparative disinfection efficiency of viruses, bacteria and spores by UV irradiation. Furthermore, the microbial quality of effluent produced by coagulation, high rate filtration (HRF) and either UV irradiation or chlorination was determined. Using low pressure collimated beam, a UV dose of 80 mWs/cm2 was needed to achieve a 3-log10 inactivation of either rotavirus SA-11 or coliphage MS2, whereas over 5-log10 inactivation of E. coli was reached with a dose of only 20 mWs/cm2. B. subtilis inactivation was found to be linear up to a dose of 40 mWs/cm2 and then a tailing up to a UV dose of 120 mWs/cm2 was observed. It is worth noting that effluent turbidity of <5 NTU did not influence the inactivation efficiency of UV irradiation. Operation of a pilot plant to treat secondary effluent by coagulation, HRF and UV disinfection at a UV dose of 80 mWs/cm2 resulted in the production of high quality effluent in compliance with the Israel standards for unrestricted irrigation (<10 CFU/100 mL faecal coliform and turbidity of <5 NTU). Sulphite reducing clostridia (SRC) were found to be more resistant than coliphages and F coliform for UV irradiation. The results of this study indicated that UV disinfection is suitable for the production of effluents for unrestricted irrigation of food crops.

Determining potential indicators of Cryptosporidium oocysts throughout the wastewater treatment process

2012 ◽  
Vol 65 (5) ◽  
pp. 875-882 ◽  
Author(s):  
H.-W. A. Cheng ◽  
M. A. Broaders ◽  
F. E. Lucy ◽  
S. E. Mastitsky ◽  
T. K. Graczyk
Keyword(s):  
Wastewater Treatment ◽  
Activated Sludge ◽  
Municipal Wastewater ◽  
Treatment Process ◽  
Giardia Duodenalis ◽  
Strong Positive Correlation ◽  
Indicator Organisms ◽  
Microbial Indicators ◽  
Cryptosporidium Oocysts ◽  
Giardia Cysts

Most research on wastewater treatment efficiency compliance focuses on physicochemical and microbial indicators; however, very little emphasis has been placed so far on determining suitable indicator organisms to predict the discharge level of pathogens from treatment plants. In this study, raw wastewater, activated sludge, and the resulting final effluents and biosolids in four municipal wastewater treatment plants (WWTPs A, B, C and D) were seasonally investigated for human-virulent water-borne pathogens Cryptosporidium parvum/hominis and Giardia duodenalis, and microsporidia (e.g. Encephalitozoon hellem, E. intestinalis, and Enterocytozoon bieneusi) between 2008 and 2009. A suite of potential microbial indicators for human-virulent protozoa and microsporidia was also determined. A combination of multiple fluorescent in situ hybridization and immunofluorescent antibody assays were applied to detect Cryptosporidium oocysts, Giardia cysts, and microsporidian spores. Escherichia coli, enterococci and Clostridium perfringens spores were cultivated in selective media. Positive correlations were found between the abundance of enterococci and E. coli and abundance of Cryptosporidium oocysts (rs > 0.47, p < 0.01) and Giardia cysts (rs > 0.44, p < 0.01) at WWTPs A–D. Cryptosporidium perfringens spores were positively correlated to Cryptosporidium oocysts (rs = 0.40, p < 0.01) and Giardia cysts (rs = 0.46, p < 0.01). There was a strong positive correlation between abundance of Giardia cysts and that of Cryptosporidium oocysts (rs > 0.89, p < 0.01). To sum up, a suite of faecal indicator bacteria can be used as indicators for the presence of Cryptosporidium oocysts and Giardia cysts in these activated-sludge systems (WWTPs A, B and C). Overall, Giardia duodenalis was noted to be the best Cryptosporidium indicator for human health in the community-based influent wastewater and throughout the treatment process.

A study about the use of chemicals in conventional tertiary treatment. Performances comparison of three municipal wastewater treatment plants and pilot plant experiences

2011 ◽  
Vol 6 (1) ◽  
Author(s):  
A. Iborra-Clar ◽  
J.A. Mendoza-Roca ◽  
A. Bes-Pií ◽  
J.J. Morenilla-Martínez ◽  
I. Bernácer-Bonora ◽  
...  
Keyword(s):  
Water Quality ◽  
Wastewater Treatment ◽  
Pilot Plant ◽  
Municipal Wastewater ◽  
Wastewater Treatment Plants ◽  
Secondary Effluent ◽  
Municipal Wastewater Treatment ◽  
Wastewater Reclamation ◽  
Valencian Region ◽  
Municipal Wastewater Treatment Plants

Rainfall diminution in the last years has entailed water scarcity in plenty of European regions, especially in Mediterranean areas. As a consequence, regional water authorities have enhanced wastewater reclamation and reuse. Thus, the implementation of tertiary treatments has become of paramount importance in the municipal wastewater treatment plants (WWTP) of Valencian Region (Spain). Conventional tertiary treatments consist of a physico-chemical treatment of the secondary effluent followed by sand filtration and UV radiation. However, the addition of coagulants and flocculants sometimes does not contribute significantly in the final water quality. In this work, results of 20-months operation of three WWTP in Valencian Region with different tertiary treatments (two without chemicals addition and another with chemicals addition) are discussed. Besides, experiments with a 2 m3/h pilot plant located in the WWTP Quart-Benager in Valencia were performed in order to evaluate with the same secondary effluent the effect of the chemicals addition on the final water quality. Results showed that the addition of chemicals did not improve the final water quality significantly. These results were observed both comparing the three full scale plants and in the pilot plant operation.

Micropollutants Produced by Disinfection of Wastewater Effluents*

1982 ◽  
Vol 14 (12) ◽  
pp. 45-59 ◽  
Author(s):  
R L Jolley ◽  
R B Cumming ◽  
N E Lee ◽  
J E Thompson ◽  
L R Lewis
Keyword(s):  
Wastewater Treatment ◽  
Uv Irradiation ◽  
Base Pair ◽  
Wastewater Treatment Plants ◽  
Department Of Energy ◽  
Union Carbide Corporation ◽  
Organic Constituents ◽  
Chemical Effects ◽  
Base Pair Substitution ◽  
The U.S

The principal objective of this research program was to examine the effects of disinfection by chlorine, ozone, and ultraviolet light (uv) irradiation on nonvolatile organic constituents relative to chemical effects and the formation of micropollutants. In a comparative study of highly concentrated samples of effluents from nine wastewater treatment plants, it was determined that disinfection with chlorine or ozone both destroys and produces nonvolatile organic constituents including mutagenic constituents. The chemical effects of disinfection by uv irradiation were relatively slight, although the mutagenic constituents in one effluent were eliminated by this treatment. The nine wastewater treatment plants were selected by using the following criteria: disinfection method, nature of wastewater source, type of wastewater treatment, standards for quality of treatment, and geographical location. The treatment plants varied from pilot plant and small plants [0.05 m3/s (1 Mgd)] treating principally domestic waste to large plants [4.4 m3/s (100 Mgd)] treating principally industrial waste. Four plants used only chlorine for disinfection, four used ozone for disinfection, and one used uv irradiation for disinfection. Eight treatment plants used conventional secondary or more advanced wastewater treatment, and one plant used primary treatment. The following methodology was used in this investigation: grab sample collection of 40-L samples of undisinfected and disinfected effluents; concentration of the effluents by lyophilization; high-pressure liquid chromatographic separation of nonvolatile organic constituents in effluent concentrates using uv absorbance, cerate oxidation, and fluorescence detectors; bacterial mutagenicity testing of concentrates and chromatographic fractions; and identification and characterization of nonvolatile organic constituents in mutagenic HPLC fractions. With these procedures, over 100 micropollutants were identified in the wastewater effluent concentrates. Interplant comparison revealed considerable variability in the presence of mutagenic nonvolatile organic constituents in the undisinfected effluent concentrates as well as much variability in the destruction of the mutagenic constituents and the formation of other mutagenic constituents as a result of disinfection. Moreover, the effects varied on samples collected at the same wastewater treatment plant at different periods. No micropollutants known to be mutagens were identified in the mutagenic HPLC fractions separated from the undisinfected, chlorinated, and ozonated effluent concentrates. The mutagenic activity of the nonvolatile organic constituents in one chlorinated effluent concentrate was not attributable to organic chloramines. Most of the mutagens detected in effluent concentrates are direct acting and do not require metabolic activation. Both base-pair substitution mutagens and frame-shift mutagens occurred in the wastewater concentrates, but the former type was more frequent. For many of the compounds in effluents, strain TA-1535 was more sensitive than strain TA-100 in detecting base-pair substitution mutagens. *Research sponsored by the U.S. Department of Energy and the U.S. Environmental Protection Agency. The work was carried out at the Oak Ridge National Laboratory, which is operated by the U.S. Department of Energy under contract W-7405-eng-26 with the Union Carbide Corporation.

F-specific bacteriophage as an indicator of human viruses in natural waters and sewage effluents in Northern New Zealand

1995 ◽  
Vol 31 (5-6) ◽  
pp. 231-234 ◽  
Author(s):  
Gillian D. Lewis
Keyword(s):  
New Zealand ◽  
Comparative Study ◽  
Marine Environment ◽  
Natural Waters ◽  
Sewage Effluents ◽  
Pathogenic Viruses ◽  
Human Viruses ◽  
Specific Bacteriophage ◽  
Beach Water

To assess the F-specific bacteriophage as an indicator of pathogenic viruses, a comparative study has been made of the occurrence of F-phage and human enteroviruses in sewage wastes and the marine environment. Although F-phage seemed in several respects to match pathogen behaviour, its low abundance in bathing beach water, uncertainty as to its source and other detection irregularities make its use as an indicator problematical.

WASTEWATER DISINFECTION BY UV AT TRANI MUNICIPAL PLANT

1994 ◽  
Vol 30 (4) ◽  
pp. 125-132 ◽  
Author(s):  
D. Carnimeo ◽  
E. Contini ◽  
R. Di Marino ◽  
F. Donadio ◽  
L. Liberti ◽  
...  
Keyword(s):  
Wastewater Treatment ◽  
Municipal Wastewater ◽  
Treatment Plant ◽  
Continuous Operation ◽  
Secondary Effluent ◽  
Municipal Wastewater Treatment ◽  
South Italy ◽  
Wastewater Disinfection ◽  
Pilot Investigation ◽  
Effluent Characteristics

The pilot investigation on the use of UV as an alternative disinfectant to NaOCI was started in 1992 at Trani (South Italy) municipal wastewater treatment plant (335 m3/h). The results collected after six months continuous operation enabled us to compare UV and NaOCl disinfection effectiveness on the basis of secondary effluent characteristics, quantify photoreactivation effects, evidence possible DBP formation and assess costs.

Textile Wastewater Reuse: Ozonation of Membrane Concentrated Secondary Effluent

1999 ◽  
Vol 40 (4-5) ◽  
pp. 99-105 ◽  
Author(s):  
A. Lopez ◽  
G. Ricco ◽  
R. Ciannarella ◽  
A. Rozzi ◽  
A. C. Di Pinto ◽  
...  
Keyword(s):  
Wastewater Treatment ◽  
Acute Toxicity ◽  
Wastewater Treatment Plant ◽  
Textile Industry ◽  
Nonionic Surfactants ◽  
Wastewater Reuse ◽  
Treatment Plant ◽  
Textile Wastewater ◽  
Secondary Effluent ◽  
Complete Disappearance

Among the activities appointed by the EC research-project “Integrated water recycling and emission abatement in the textile industry” (Contract: ENV4-CT95-0064), the effectiveness of ozone for improving the biotreatability of recalcitrant effluents as well as for removing from them toxic and/or inhibitory pollutants has been evaluated at lab-scale. Real membrane concentrates (pH=7.9; TOC=190 ppm; CDO=595 ppm; BOD5=0 ppm; Conductivity=5,000 μS/cm; Microtox-EC20=34%) produced at Bulgarograsso (Italy) Wastewater Treatment Plant by nanofiltering biologically treated secondary textile effluents, have been treated with ozonated air (O3conc.=12 ppm) over 120 min. The results have indicated that during ozonation, BOD5 increases from 0 to 75 ppm, whereas COD and TOC both decrease by about 50% and 30 % respectively. As for potentially toxic and/or inhibitory pollutants such as dyes, nonionic surfactants and halogenated organics, all measured as sum parameters, removals higher than 90% were achieved as confirmed by the complete disappearance of acute toxicity in the treated streams. The only ozonation byproducts searched for and found were aldehydes whose total amount continuously increased in the first hour from 1.2 up to 11.8 ppm. Among them, formaldehyde, acetaldehyde, glyoxal, propionaldehyde, and butyraldehyde were identified by HPLC.

scholarly journals Occurrence of SARS-CoV-2 RNA in Six Municipal Wastewater Treatment Plants at the Early Stage of COVID-19 Pandemic in The United States

Pathogens ◽  
2021 ◽  
Vol 10 (7) ◽  
pp. 798
Author(s):  
Samendra P. Sherchan ◽  
Shalina Shahin ◽  
Jeenal Patel ◽  
Lauren M. Ward ◽  
Sarmila Tandukar ◽  
...  
Keyword(s):  
United States ◽  
Wastewater Treatment ◽  
Municipal Wastewater ◽  
Wastewater Treatment Plants ◽  
Quantitative Polymerase Chain Reaction ◽  
The United States ◽  
Secondary Effluent ◽  
Untreated Wastewater ◽  
Treatment Processes ◽  
Wastewater Samples

In this study, we investigated the occurrence of Severe Acute Respiratory Syndrome Coronavirus-2 (SARS-CoV-2) RNA in primary influent (n = 42), secondary effluent (n = 24) and tertiary treated effluent (n = 34) collected from six wastewater treatment plants (WWTPs A–F) in Virginia (WWTP A), Florida (WWTPs B, C, and D), and Georgia (WWTPs E and F) in the United States during April–July 2020. Of the 100 wastewater samples analyzed, eight (19%) untreated wastewater samples collected from the primary influents contained SARS-CoV-2 RNA as measured by reverse transcriptase quantitative polymerase chain reaction (RT-qPCR) assays. SARS-CoV-2 RNA were detected in influent wastewater samples collected from WWTP A (Virginia), WWTPs E and F (Georgia) and WWTP D (Florida). Secondary and tertiary effluent samples were not positive for SARS-CoV-2 RNA indicating the treatment processes in these WWTPs potentially removed SARS-CoV-2 RNA during the secondary and tertiary treatment processes. However, further studies are needed to understand the log removal values (LRVs) and transmission risks of SARS-CoV-2 RNA through analyzing wastewater samples from a wider range of WWTPs.

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