Comparison of advanced disinfecting methods for municipal wastewater reuse in agriculture

2000 ◽  
Vol 42 (1-2) ◽  
pp. 215-220 ◽  
Author(s):  
L. Liberti ◽  
A. Lopez ◽  
M. Notarnicola ◽  
N. Barnea ◽  
R. Pedahzur ◽  
...  
Keyword(s):  
Hydrogen Peroxide ◽  
Municipal Wastewater ◽  
Wastewater Reuse ◽  
Bactericidal Effect ◽  
Pilot Scale ◽  
Fecal Coliforms ◽  
Pronounced Increase ◽  
E Coli ◽  
Laboratory Results ◽  
Virucidal Effect

The experimental investigation carried out jointly by Italian and Israeli teams within a 3-year research project funded by the European Commission on alternative disinfection methods for reusing municipal wastewater in agriculture is described. Disinfection effectiveness of UV rays, ozone (O3) and peracetic acid (PAA) at pilot scale and hydrogen peroxide (H2O2) plus silver (Ag+) or copper (Cu+2) at laboratory scale was compared for tertiary municipal effluents. Pilot plant results indicated that the well known California limit (2 CFU/100ml of Total Coliforms) was effectively met with reasonable UV doses of 100–160 mWs/cm2 or with exceedingly high PAA dosage (400 PPM); the related WHO microbial guideline (1,000 CFU/100 ml of Fecal Coliforms) was easily met with all three disinfectants (UV, PAA, O3); maximum log-inactivation values were ≥5 for UV and PAA and ≤4 for O3. Laboratory results with tertiary effluents demonstrated that Ag+ was able to inactivate target bacteria (E. coli-B, E. coli-K12), while H2O2 was more effective than Ag+ against MS-2 phages. Copper (250 μg/l) had no bactericidal effect but possessed an appreciable virucidal effect. When hydrogen peroxide and copper were combined, a pronounced increase in both bactericidal and virucidal effects was obtained.

scholarly journals Effectiveness of Solar Driven Anodic Oxidation and Uv Methods for Disinfection of Municipal Wastewater in India

2019 ◽  
Vol 8 (4) ◽  
pp. 12007-12010
Keyword(s):  
Anodic Oxidation ◽  
Energy Demand ◽  
Municipal Wastewater ◽  
Pilot Scale ◽  
Integrated Treatment ◽  
Fecal Coliforms ◽  
Wastewater Management ◽  
Indicator Organisms ◽  
E Coli ◽  
Disinfection Methods

This paper presents the performance of the two disinfection methods experimented at a pilot scale level as part of an integrated treatment system involving Anaerobic Digestion (UASB Process) followed by the Constructed Wetlands (CW) for municipal wastewater treatment under Indian conditions. The disinfection methods were installed in parallel at a pilot scale levels involving solar driven Anodic Oxidation (AO) and Ultra-violet (UV). The systems were implemented within the Indo-Euro Research Project “SWINGS” at three places in India namely, Aligarh, Kalyani and Amarkantak, with aim to develop / deploy sustainable wastewater management. This study mainly focused on three microbial groups, total coliforms (TC), fecal coliforms (FC) and E-coli. Both the systems, AO and UV were operated at a very less energy demand. This was in the range 0.25 to 0.30 kWh/m3. The energy requirements was met with the help of roof top solar panels. The raw municipal wastewater contained TC, FC and E. coli in the range of 105 to 107 CFU/100ml. It was found that about 3-log unit indicator organisms were removed in the UASB & CWs. The effluent of CWs were equally fed to the two parallel disinfection systems, AO and UV. Results indicate that UV has shown better performance. The final effluent of AO contains average (TC 42.0±39.2, FC 16.1±13.2, E-coli 5.6±6.3) CFU/100 ml whereas UV effluent was TC 6.6±4.1, FC 1.2±1.0, E-coli 0.1 ±0.3 CFU/100 ml. The study concludes that solar driven disinfection systems like AO and UV are very effective for small flow rates and offers a promising way to energy scarce regions or in remote areas.

Potentiation by L-cysteine of the bactericidal effect of hydrogen peroxide in Escherichia coli

1982 ◽  
Vol 152 (1) ◽  
pp. 81-88
Author(s):  
E H Berglin ◽  
M B Edlund ◽  
G K Nyberg ◽  
J Carlsson
Keyword(s):  
Escherichia Coli ◽  
Hydrogen Peroxide ◽  
Metal Ion ◽  
Chelating Agent ◽  
Fold Increase ◽  
Bactericidal Effect ◽  
Anaerobic Conditions ◽  
Dna Breakage ◽  
E Coli ◽  
K 12

Under anaerobic conditions an exponentially growing culture of Escherichia coli K-12 was exposed to hydrogen peroxide in the presence of various compounds. Hydrogen peroxide (0.1 mM) together with 0.1 mM L-cysteine or L-cystine killed the organisms more rapidly than 10 mM hydrogen peroxide alone. The exposure of E. coli to hydrogen peroxide in the presence of L-cysteine inhibited some of the catalase. This inhibition, however, could not fully explain the 100-fold increase in hydrogen peroxide sensitivity of the organism in the presence of L-cysteine. Of other compounds tested only some thiols potentiated the bactericidal effect of hydrogen peroxide. These thiols were effective, however, only at concentrations significantly higher than 0.1 mM. The effect of L-cysteine and L-cystine could be annihilated by the metal ion chelating agent 2,2'-bipyridyl. DNA breakage in E. coli K-12 was demonstrated under conditions where the organisms were killed by hydrogen peroxide.

Antimicrobial Effects of Novel H2O2-Ag+ Complex on Membrane Damage to Staphylococcus aureus,Escherichia coli and Salmonella typhimurium

2021 ◽  
Author(s):  
Bing Han ◽  
Xiaoyu Han ◽  
Mengmeng Ren ◽  
Yilin You ◽  
Jicheng Zhan ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Hydrogen Peroxide ◽  
Staphylococcus Aureus ◽  
Salmonella Typhimurium ◽  
Membrane Damage ◽  
Bactericidal Effect ◽  
E Coli ◽  
Bacterial Cell Membrane ◽  
Time Kill ◽  
Environmental Disinfection

Diseases caused by harmful microorganisms pose a serious threat to human health. Safe and environment-friendly disinfectants are, therefore, essential in preventing and controlling such pathogens. This study aimed to investigate the antimicrobial activity and mechanism of a novel hydrogen peroxide and silver (H 2 O 2 -Ag + ) complex (HSC) in combatting Staphylococcus aureus ATCC 29213, Escherichia coli O157:H7 NCTC 12900 and Salmonella typhimurium SL 1344. The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) values against S. aureus were found to be 0.014 % H 2 O 2 -3.125 mg/L Ag + , while 0.028 % H 2 O 2 -6.25 mg/L Ag + for both E. coli and S. typhimurium . Results of the growth curve assay and time-kill trial suggest that the HSC could inhibit the growth of the tested bacteria, as 99.9 % of viable cells were killed following treatment at the 1 MIC for 3 h. Compared with Oxytech D10 disinfectant (0.25 % H 2 O 2 -5 mg/L Ag + ), the HSC exhibited better antibacterial efficacy at a lower concentration (0.045 % H 2 O 2 -10 mg/L Ag + ). The mechanism of antibacterial action of HSC was found including the disruption of the bacterial cell membrane, followed by entry into the bacteria cell to reduce intracellular adenosine triphosphate (ATP) concentration, and inhibit the activity of antioxidases, superoxide dismutase (SOD) and catalase (CAT). The enhanced bactericidal effect of hydrogen peroxide combined with silver indicates a potential for its application in environmental disinfection, particularly in the food industry.

scholarly journals Membrane-Based Processes Used in Municipal Wastewater Treatment for Water Reuse: State-Of-The-Art and Performance Analysis

Membranes ◽  
2020 ◽  
Vol 10 (6) ◽  
pp. 131 ◽  
Author(s):  
Jiaqi Yang ◽  
Mathias Monnot ◽  
Lionel Ercolei ◽  
Philippe Moulin
Keyword(s):  
Water Reuse ◽  
Public Perception ◽  
Municipal Wastewater ◽  
Wastewater Reuse ◽  
Pilot Scale ◽  
Economic Feasibility ◽  
Municipal Wastewater Treatment ◽  
Removal Capacity ◽  
Treatment Processes ◽  
Alternative Water

Wastewater reuse as a sustainable, reliable and energy recovery concept is a promising approach to alleviate worldwide water scarcity. However, the water reuse market needs to be developed with long-term efforts because only less than 4% of the total wastewater worldwide has been treated for water reuse at present. In addition, the reclaimed water should fulfill the criteria of health safety, appearance, environmental acceptance and economic feasibility based on their local water reuse guidelines. Moreover, municipal wastewater as an alternative water resource for non-potable or potable reuse, has been widely treated by various membrane-based treatment processes for reuse applications. By collecting lab-scale and pilot-scale reuse cases as much as possible, this review aims to provide a comprehensive summary of the membrane-based treatment processes, mainly focused on the hydraulic filtration performance, contaminants removal capacity, reuse purpose, fouling resistance potential, resource recovery and energy consumption. The advances and limitations of different membrane-based processes alone or coupled with other possible processes such as disinfection processes and advanced oxidation processes, are also highlighted. Challenges still facing membrane-based technologies for water reuse applications, including institutional barriers, financial allocation and public perception, are stated as areas in need of further research and development.

The efficacy of long-lasting residual drinking water disinfectants based on hydrogen peroxide and silver

2000 ◽  
Vol 42 (1-2) ◽  
pp. 293-298 ◽  
Author(s):  
R. Pedahzur ◽  
D. Katzenelson ◽  
N. Barnea ◽  
O. Lev ◽  
H.I. Shuval ◽  
...  
Keyword(s):  
Hydrogen Peroxide ◽  
Heat Shock ◽  
Stress Responses ◽  
Heat Shock Response ◽  
Chemical Properties ◽  
Bactericidal Effect ◽  
Physiological Effects ◽  
Mechanisms Of Toxicity ◽  
E Coli ◽  
Shock Response

The aim of the present work was to evaluate the disinfectant capacity and the possible fields of application of a combined silver and hydrogen peroxide (HP) water disinfectant. The findings demonstrated the high bactericidal action of silver (on E. coli) and its relatively ineffective virucidal effect (on MS-2 phage). HP was found to have a small bactericidal effect and a mild virucidal one. When combined, silver and HP usually exhibited a synergistic action on the viability of E. coli and on the luminescence of recombinant luminescent E. coli. In some instances, the combined bactericidal effects were 1000-fold higher than the sum of the separate ones. No increased virucidal action was observed. The biocidal action of the combination generally increased with increasing temperature and pH, and decreased in secondary and tertiary effluents. The physiological effects and mechanisms of toxicity of HP, silver and their combinations, were assessed by monitoring the induction of stress promoters upon exposure to the active agents, and by assessing the sensitivity of E. coli mutated in major stress responses to HP, silver and their combinations. The results showed that HP induced a wide array of stress responses, that both silver and HP induced promoters regulated by the heat shock response, and that the dnaK promoter (regulated by the heat shock response) was synergistically induced. The mutant sensitivity tests showed that bacteria deficient in the ability to activate central cellular stress responses (SOS, heat shock, stationary phase, oxidative) were hypersensitive to both HP and silver. These results imply that cellular proteins, and possibly the DNA, are the cellular moieties chiefly affected. The above findings suggest that the potentiated effect of HP and silver is a metabolically dependant/related process that stems from a combination and/or accumulation of physiological effects exerted by the active ingredients. The physico-chemical properties of the combined disinfectant, and its disinfection capacity, points to its potential application as a long-term secondary residual disinfectant for water of relatively high quality.

A pilot-scale study of peracetic acid and ultraviolet light for wastewater disinfection

2019 ◽  
Vol 5 (8) ◽  
pp. 1453-1463 ◽  
Author(s):  
Abdulrahman H. Hassaballah ◽  
Jeremy Nyitrai ◽  
Christine H. Hart ◽  
Ning Dai ◽  
Lauren M. Sassoubre
Keyword(s):  
Ultraviolet Light ◽  
Peracetic Acid ◽  
Pilot Scale ◽  
Fecal Coliforms ◽  
Treated Wastewater ◽  
E Coli ◽  
Wastewater Disinfection ◽  
Somatic Coliphage

At the pilot-scale, peracetic acid effectively inactivates fecal coliforms,E. coliandEnterococcusspp. in secondary and tertiary treated wastewater. The addition of UV to PAA treatment increases inactivation of somatic coliphage.

Pilot study of SBR biological treatment and microfiltration for reclamation and reuse of municipal wastewater

2000 ◽  
Vol 42 (1-2) ◽  
pp. 263-268 ◽  
Author(s):  
R. Messalem ◽  
A. Brenner ◽  
S. Shandalov ◽  
Y. Leroux ◽  
P. Uzlaner ◽  
...  
Keyword(s):  
Water Reuse ◽  
Municipal Wastewater ◽  
Batch Reactor ◽  
Groundwater Resources ◽  
Oxygen Demand ◽  
Pilot Scale ◽  
Fecal Coliforms ◽  
Wastewater Reclamation ◽  
Two Stage ◽  
Stage Scheme

In Israel the shortage of water and concern for the quality of groundwater resources have led to an awareness that a national wastewater reclamation program must be developed. Such a program could cover a major part of the agricultural water demand and could facilitate disposal of effluents without health hazards or environmental problems. A two-stage pilot-scale system comprising secondary sequencing batch reactor (SBR) treatment and tertiary microfiltration was operated for the treatment of Beer-Sheva municipal wastewater. The self-cleaning, continuous microfiltration system comprised a filter module made up of hollow fiber microporous membranes, with a pore size distribution of less than 0.1 μm, encapsulated into a bundle. The unit, which has a nominal filtration area of 4 m2, can treat 4–5 m3 of sewage per day, at a nominal rate of about 500 L/h. SBR treatment of the raw sewage produced an effluent with a biochemical oxygen demand (BOD) of <20 mg/L and total suspended solids (TSS) of <20 mg/L. Further treatment by microfiltration resulted in a BOD <5 mg/L, TSS <1 mg/L and turbidity <0.2 nephelometric turbidity units (NTU). Bacterial counts showed 6-log removal of coliforms and fecal coliforms. These results indicate that the two-stage scheme is capable of producing an effluent that meets or even surpasses the requirements for unrestricted water reuse for agriculture.

scholarly journals Analyses of the Effect of Peptidoglycan on Photocatalytic Bactericidal Activity Using Different Growth Phases Cells of Gram-Positive Bacterium and Spheroplast Cells of Gram-Negative Bacterium

Catalysts ◽  
2021 ◽  
Vol 11 (2) ◽  
pp. 147
Author(s):  
Akane Saikachi ◽  
Kotone Sugasawara ◽  
Tomonori Suzuki
Keyword(s):  
Hydrogen Peroxide ◽  
Hydroxyl Radical ◽  
Survival Rates ◽  
Bactericidal Effect ◽  
Growth Phases ◽  
Intact Cells ◽  
E Coli ◽  
Bactericidal Effects ◽  
Photocatalytic Bactericidal Activity ◽  
Peptidoglycan Layer

We conducted photocatalytic experiments focusing on the peptidoglycan layer to elucidate the details of the mechanism of photocatalytic sterilization. The previous study of our laboratory suggested that the presence of the peptidoglycan layer increases the bactericidal effect. To further verify it, the following experiments were performed: experiments on cells with different peptidoglycan layer thickness used Lactobacillus plantarum cells with different growth phases, experiments on cells with the thin peptidoglycan layer used Escherichia coli cells and spheroplast cells from which the peptidoglycan layer was removed from E. coli cells. The bactericidal effects increased as the growth progresses of L. plantarum. It was confirmed by TEM that the thickness of the peptidoglycan layer increased with cell growth. The survival rates of E. coli intact cells were significantly lower than those of spheroplast cells. These results strongly suggest that the peptidoglycan layer enhances the photocatalytic bactericidal effect. As a result of allowing the photocatalytic reaction to act on peptidoglycan, the amount of hydroxyl radical was smaller, and the amount of hydrogen peroxide was higher than in the absence of peptidoglycan. It is suggested that peptidoglycan may convert produced hydroxyl radical to hydrogen peroxide.

scholarly journals Microbiological and physicochemical evaluation of the effluent quality in a membrane bioreactor system to meet the legislative limits for wastewater reuse

2017 ◽  
Vol 76 (7) ◽  
pp. 1796-1804 ◽  
Author(s):  
Konstantinos Azis ◽  
Charalampos Vardalachakis ◽  
Spyridon Ntougias ◽  
Paraschos Melidis
Keyword(s):  
Membrane Bioreactor ◽  
Municipal Wastewater ◽  
Wastewater Reuse ◽  
Oxygen Demand ◽  
Pilot Scale ◽  
Transmembrane Pressure ◽  
Permeate Flux ◽  
Effluent Quality ◽  
Mediterranean Countries

The aim of this study was to assess the efficacy and effluent quality of a pilot-scale intermittently aerated and fed, externally submerged membrane bioreactor (MBRes) treating municipal wastewater. The effluent quality of the MBRes was evaluated regarding system ability to comply with the Greek legislative limits for restricted and unrestricted wastewater reuse. The average permeate flux was 13.9 L m−2 h−1, while the transmembrane pressure remained above the level of −110 mbar. Experimental data showed that biochemical oxygen demand, chemical oxygen demand, total nitrogen, PO43−- P and total suspended solids removal efficiencies were 97.8, 93.1, 89.6, 93.2 and 100%, respectively, whereas turbidity was reduced by 94.1%. Total coliforms and Escherichia coli were fully eliminated by ultrafiltration and disinfection methods, such as chlorination and ultraviolet radiation. In agreement with the Greek legislation (Joint Ministerial Decree 145116/11) and the guidelines recommended for the Mediterranean countries, the disinfected effluent of the MBRes system can be safely reused directly for urban purposes.

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