The influence of different substrates on enhanced biological phosphorus removal in a sequencing batch reactor

1997 ◽  
Vol 35 (1) ◽  
pp. 75-80 ◽  
Author(s):  
R. Tasli ◽  
N. Artan ◽  
D. Orhon
Keyword(s):  
Phosphorus Removal ◽  
Sequencing Batch Reactor ◽  
Batch Reactor ◽  
The Other ◽  
Organic Substrates ◽  
Relative Importance ◽  
Enhanced Biological Phosphorus Removal ◽  
Biological Phosphorus Removal ◽  
Batch Tests ◽  
Biological Phosphorus

All models of enhanced biological phosphorus removal (EBPR) define fermentable readily biodegradable substrate, without emphasizing the significance of its composition and the relative importance of different substrates. On the other hand, it is also known that substrates like glucose may be utilized without requiring poly-P energy, a phenomenon which deteriorates the EBPR performance. This paper reports an experimental study evaluating the effect of different organic substrates and their combinations on EBPR, in a sequencing batch reactor. Experimental data show that the EBPR efficiency is significantly affected by the increase of the glucose fraction in the feed, due to the probable dominance of G bacteria. Results of anaerobic batch tests also support this evaluation.

Bacteria and Protozoa Population Dynamics in Biological Phosphate Removal Systems

1994 ◽  
Vol 29 (7) ◽  
pp. 109-117 ◽  
Author(s):  
J. S. Čech ◽  
P. Hartman ◽  
M. Macek
Keyword(s):  
Population Dynamics ◽  
Phosphorus Removal ◽  
Sequencing Batch Reactor ◽  
Batch Reactor ◽  
Sole Source ◽  
Phosphate Removal ◽  
Enhanced Biological Phosphorus Removal ◽  
Biological Phosphorus Removal ◽  
System Collapse ◽  
Biological Phosphorus

Population dynamics of polyphosphate-accumulating bacteria (PP bacteria) was studied in a laboratory sequencing batch reactor simulating anaerobic-oxic sludge system. The competition between PP bacteria and another microorganism (“G bacteria”) for anaerobic-oxic utilization of acetate as the sole source of organic carbon was observed. The competition was found to be seriously influenced by protozoan and metazoan grazing: Predation-resistant “G bacteria” forming large compact flocs outcompeted PP bacteria. Several breakdowns of enhanced biological phosphorus removal were observed. The first one was related to the development of an euglenid flagellate Entosiphon sulcatus and attached ciliates Vorticella microstoma and V. campanula. The second system collapse was connected with a rapid proliferation of rotifers. An alternative-prey predation was thought to be a mechanism of PP bacteria elimination.

Biological phosphorus removal with different organic substrates in an anaerobic/aerobic sequencing batch reactor

1997 ◽  
Vol 35 (1) ◽  
pp. 161-168 ◽  
Author(s):  
A. Carucci ◽  
M. Majone ◽  
R. Ramadori ◽  
S. Rossetti
Keyword(s):  
Phosphorus Removal ◽  
Sequencing Batch Reactor ◽  
Batch Reactor ◽  
Energy Sources ◽  
Organic Substrates ◽  
Biological Phosphorus Removal ◽  
Anaerobic Conditions ◽  
And Storage ◽  
Biological Phosphorus ◽  
Hydrolysis Of

Experimental work was carried out with a lab-scale SBR, whose operation was divided into three runs: I run (glucose + acetate feed, 3′ fill), II run (only glucose feed, 3′ fill), III run (only glucose feed, 60′ fill). The results show that the bacteria growing in alternating anaerobic/aerobic systems can remove organic substrates under anaerobic conditions even without using the hydrolysis of polyphosphates or the transformation of glycogen into poly-hydroxyalkanoates as the energy sources for the uptake and storage of substrate. However, in the case of acetate plus glucose feed, EBPR can be steadily established with typical PP-bacteria like metabolism even if most of the COD removal takes place under not truly anaerobic conditions.

Morphological characteristics of microbial sludge performing enhanced biological phosphorus removal in a sequencing batch reactor fed with glucose as sole carbon source

2000 ◽  
Vol 41 (12) ◽  
pp. 79-84 ◽  
Author(s):  
C. O. Jeon ◽  
D. S. Lee ◽  
J. M. Park
Keyword(s):  
Phosphorus Removal ◽  
Sole Carbon Source ◽  
Sequencing Batch Reactor ◽  
Batch Reactor ◽  
Morphological Characteristics ◽  
Enhanced Biological Phosphorus Removal ◽  
Biological Phosphorus Removal ◽  
Electron Microscopic ◽  
Biological Phosphorus ◽  
Polyphosphate Granules

Electron microscopic analysis was used to analyse the morphological characteristics of microbial sludge performing enhanced biological phosphorus removal (EBPR) in an anaerobic/aerobic sequencing batch reactor (SBR) fed with glucose as the sole carbon source. The amounts of phosphate release and uptake during the SBR cycle gradually increased with operation time and complete EBPR was achieved after about 90 days. Scanning electron microscopy (SEM) showed that the initial sludge inoculated into the SBR consisted of various microorganisms such as coccus-, rod- and bacillus-shaped bacteria, but after extended operation (more than 650 days) perpendicular cuboidal bacteria of eight coccus-shaped cells dominated the microbial sludge in the SBR reactor. The cell size of the cuboidal bacteria was about 0.7 μm in diameter. In the sludge, coccus- and rod-shaped bacteria also existed but at much lower frequency. Transmission electron microscopy (TEM) also revealed that the cuboidal bacteria dominated the sludge, but they did not contain polyphosphate granules or glycogen inclusions. The rod-shaped bacteria did not contain polyphosphate granules or glycogen inclusions either. Only coccus-shaped bacteria with a diameter of about 1.2 μm contained small black polyphosphate granules and a large white inclusion. Based on previously proposed metabolic pathways and electron microscopic results, it was inferred that the dominating cuboidal bacteria were lactic acid producing organisms (LPO) and the coccus-shaped bacteria were lactate-using phosphorus accumulating organisms (PAO).

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