scholarly journals Microbial wash water quality on dairy farms from Galicia (NW Spain)

2019 ◽  
Vol 19 (8) ◽  
pp. 2214-2221
Author(s):  
F. J. Saavedra ◽  
E. Yus ◽  
F. J. Diéguez
Keyword(s):  
Water Samples ◽  
Membrane Filtration ◽  
Dairy Farms ◽  
Total Coliform ◽  
Faecal Coliform ◽  
Wash Water ◽  
Nw Spain ◽  
Filtration Method ◽  
Bacterial Groups ◽  
Faecal Streptococci

Abstract This study evaluated the microbiological quality of wash water used in dairy farms from Galicia (NW Spain) based on the total coliform, faecal coliform and faecal streptococci counts. The paper aimed to identify geographical areas that are at a high risk for bacterial contamination of wash water and to study the temporal distribution of positive samples. Water samples from 120 farms, whose water supply came from a private well, were tested for the presence of the three mentioned bacterial groups by the membrane filtration method. To investigate whether the presence of positive herds for a particular bacterial group in water samples was higher in some areas or during a specific time period, data were tested using the Bernoulli model. In farms without a purification system, the prevalence of positive samples for total coliform, faecal coliform and faecal streptococci was 36.7%, 13.3% and 24.5%, respectively. In farms using hydrogen peroxide, these figures were 15.4%, 7.7% and 7.7%, respectively. In farms using chlorine or ultraviolet radiation all the samples were negative for the three bacterial groups. The spatial analysis identified one statistically significant spatial cluster of herds with positive samples of faecal streptococci in an area characterized by a high cattle density. Moreover, one most likely temporal cluster was identified for each of the three groups between July and August 2017, which were the driest months in the studied area.

scholarly journals Investigations on Bacteriological Quality of Tap Water Sources within the University of Port Harcourt

2019 ◽  
pp. 1-5
Author(s):  
H. O. Stanley ◽  
C. J. Ugboma ◽  
P. C. Uzoaru
Keyword(s):  
Water Samples ◽  
Membrane Filtration ◽  
Heterotrophic Bacteria ◽  
Tap Water ◽  
Total Coliform ◽  
Water Sources ◽  
Filtration Method ◽  
Port Harcourt ◽  
The University

In this study, the sanitary quality of tap water sources within the University of Port Harcourt was investigated in order to determine its suitability for drinking and other domestic applications. Eight composite samples of tap water were collected from Eight different locations within the three campuses of the University of Port Harcourt using 300 ml-capacity sterile containers. These locations included NDDC Hostel, Sports Hostel, Medical Hostel, NUH Hostel, Dan Etete Hostel, Delta A Hostel, Delta B Hostel and Choba Campus. After collection, water samples were taken to the laboratory for enumeration and identification of Total heterotrophic bacteria (THB), Total coliform (TC) and Faecal coliform (FC) using the membrane filtration method. Result showed that the average THB, TC and FC counts recorded in the tap water samples across the eight locations ranged from 12.4 CFU to 36.7 CFU, 4.3 CFU to 10.1 CFU and 2.8 CFU to 5.2 CFU per 100ml respectively. Bacterial isolates were identified as probably belonging to genera such as Bacillus spp., Klebsiella spp., Enterobacter spp., Staphylococcus spp., Proteus spp., Citrobacter spp., Serratia spp. and Escherichia spp., respectively. Samples from Delta B hostel and Choba campus recorded the lowest and highest THB, TC and FC counts respectively. According to WHO standard, the result of THB, TC and FC counts indicated that the tap water in all eight locations were above the limit acceptable for drinking but within acceptable limit for other domestic use such as bathing and washing.

An Improved Membrane Filtration Method for Enumeration of Faecal Coliforms and E. Coli by a Fluorogenic β-Glucuronidase Assay

1993 ◽  
Vol 27 (3-4) ◽  
pp. 267-270 ◽  
Author(s):  
M. T. Augoustinos ◽  
N. A. Grabow ◽  
B. Genthe ◽  
R. Kfir
Keyword(s):  
Escherichia Coli ◽  
Water Samples ◽  
Membrane Filtration ◽  
Faecal Coliforms ◽  
Environmental Waters ◽  
Filtration Method ◽  
E Coli ◽  
Wide Range ◽  
Pure Cultures ◽  
Glucuronidase Assay

A fluorogenic β-glucuronidase assay comprising membrane filtration followed by selective enumeration on m-FC agar at 44.5°C and further confirmation using tlie 4-metliylumbelliferyl-β-D-glucuronide (MUG) containing medium was evaluated for the detection of Escherichia coli in water. A total of 200 typical blue and non-typical blue colonies were isolated from sea and fresh water samples using initial selective enumeration on m-FC agar. Pure cultures of the selected colonies were further tested using the MUG assay and identified using the API 20E method. Of the colonies tested which were shown to be positive using the MUG assay 99.4% were Escherichia coli. The results of this study indicate the combination of the m-FC method followed by the MUG assay to be highly efficient for the selection and confirmation of E. coli from a wide range of environmental waters.

scholarly journals Assessment of Giardia and Cryptosporidium Assemblages/ Species and Their Viability in Potable Tap Water in Beni-Suef, Egypt Using Nested PCR/RFLP and Staining

2019 ◽  
Author(s):  
Doaa HAMDY ◽  
Ayman El-BADRY ◽  
Wegdan ABD EL WAHAB
Keyword(s):  
Water Samples ◽  
Rural Areas ◽  
Nested Pcr ◽  
Membrane Filtration ◽  
Tap Water ◽  
Significant Risk ◽  
Waterborne Diseases ◽  
Filtration Method ◽  
Pcr Rflp ◽  
Blue Stain

Background: The protozoan Giardia and Cryptosporidium are responsible for most water-borne diseases all over the world. The extent and number of outbreaks of waterborne diseases suggests a significant risk of their potential transmission via drinking water. This study aimed to document the prevalence and viability of Giardia and Cryptosporidium (oo) cysts in tap water samples in Beni-Suef Governorate, Egypt and to detect the predominant Giardia and Cryptosporidium assemblages/species using nested PCR/ Restriction Fragment Length Polymorphism (RFLP) confirmed by further sequencing of positive samples. Methods: A total of 80 tap water samples were collected throughout a year from four big centers and filtered using the membrane filtration method. Samples were stained by Lugol’s iodine, Modified Zeihl-Neelsen (MZN) (to detect prevalence) and trypan blue stain (to detect viability). Nested PCR-RFLP and sequencing were used for molecular characterizations and genotyping of the detected Giardia and Cryptosporidium. Results: Giardia and Cryptosporidium DNA was detected in 20 (25%) and 29 (36.3%) samples respectively, with predominance of Giardia assemblage B (85%) and C. hominis (75.9%). The prevalence and viability of both parasites (oo) cysts showed seasonality which peaked in summer and were greater in Beba center and in rural areas. Conclusion: To our knowledge, no studies have been done in these areas before. The anthroponotic transmission has an important role in giardiasis and crypto­sporidiosis epidemiology in this studied area.

scholarly journals Enumeration of Total and Faecal Coliform Bacteria in Drinking Water of Khairpur City

1970 ◽  
Vol 24 (2) ◽  
pp. 163-165 ◽  
Author(s):  
Abdul Hussain Shar ◽  
Yasmeen F Kazi ◽  
Miandad Zardari ◽  
Irshad Hussain Soomro
Keyword(s):  
Water Quality ◽  
Drinking Water ◽  
Membrane Filtration ◽  
Total Coliform ◽  
Faecal Coliform ◽  
World Health ◽  
Source Distribution ◽  
Coliform Bacteria ◽  
Residual Chlorine ◽  
Health Organization

Total coliform (TC) and faecal coliform (FC) bacteria were analyzed in drinking water of Khairpur city. Ninty samples were collected from main reservoir (source), distribution line and consumer taps. pH and residual chlorine of water samples were also determined. For bacteriological analysis inductively membrane filtration (MF) method was used for total coliform (TC) as well as faecal (FC) coliform bacteria. All samples were found contaminated with total coliform (TC) and faecal coliform (FC) and the counts were higher than the maximum microbial contaminant level (MMCL) established by World Health Organization (WHO). It was observed that pH was within the limits of WHO standard (6.5-8.5). The residual chlorine was not detected in any sample of drinking water. Bacteriologically the water quality of the drinking water is unsatisfactory.Keywords: Coliform, Escherichia coli, Water quality, Contamination, SanitationDOI: http://dx.doi.org/10.3329/bjm.v24i2.1266

scholarly journals Bacteriological Quality Analysis of Water in Storage Tanks in A University Community, South Southern - Nigeria

2021 ◽  
Vol 6 (3) ◽  
pp. 16-23
Author(s):  
Ofonime M. Ogba ◽  
Oluwayemisi A. Olorode ◽  
Mary P. Okon ◽  
Nse O. Umoh
Keyword(s):  
Escherichia Coli ◽  
Water Samples ◽  
Bacterial Load ◽  
Bacterial Count ◽  
Total Coliform ◽  
Nutrient Agar ◽  
Quality Analysis ◽  
Storage Tanks ◽  
Filtration Method ◽  
Coliform Count

The use of unsafe water supplies and microbial contaminated water may pose serious health challenge to users. The aim of the study was to determine the level of bacterial contamination of the various water sources and the suitability for human use and consumption in University of Calabar Community, Nigeria. A total of 30 water samples were obtained from the University storage tanks and analysed for the presence of bacteria. The level of feacal coliform count, total coliform count and heterotrophic bacterial count was analysed using membrane filtration method and standard culture method on a differential and selective media. The samples were cultured on MacConkey and Nutrient agar. The isolates obtained from the above media were subcultured into slants of nutrient agar. Isolates were subjected to Gram staining and biochemical tests. The feacal coliform, Escherichia coli was isolated from all the water samples. Total coliform counts ranged from 1cfu/ml - 92cfu/ml while total heterotrophic bacterial count ranged from 1cfu/ml to 161cfu/ml. The bacteria species isolated were Escherichia coli, Klebsiella pneumoniae, Staphylococcus aureus, Pseudomonas aeruginosa, Streptococcus species, Enterobacter aerogenes, Coagulase negative Staphylococcus, Proteus species, Lactobacillus species and Listeria species. The results obtained from this study have shown high level of bacterial load which makes the water unsuitable for human consumption. Further treatment may be needed as the bacteria isolates from the water samples point to feacal contamination which may be due to inadequate treatment of water, contamination while in the storage tanks or passage through contaminated pipes supplying the community. The consumers may be at high risks of enteric bacterial infections.

scholarly journals Comparative Microbiological Assessment of Drinking Water Collected from Different Areas of Kathmandu Valley

2019 ◽  
Vol 6 ◽  
pp. 39-43
Author(s):  
Aman Thapa Magar ◽  
Mamata Khakurel ◽  
Shree Laxmi Pandey ◽  
Kalyan Subedi ◽  
Umesh Kaji Manandhar ◽  
...  
Keyword(s):  
Ground Water ◽  
Water Samples ◽  
Membrane Filtration ◽  
Tap Water ◽  
Bacterial Load ◽  
Total Coliform ◽  
Bottled Water ◽  
Plate Count ◽  
Aerobic Plate Count ◽  
Filtration Technique

Objectives: This study was aimed to evaluate different water samples in terms of Total bacterial load and Total Coliform Load in comparison with different parameters such as pH, type of samples, chlorination status, turbidity, temperature and collection areas. Methods: Altogether 250 water samples were collected; 110(44%) were ground water samples, 60(24%) were public tap water samples and 80(32%) were bottled water samples. Total Coliform load was evaluated using Membrane Filtration Technique and Total Bacterial Load was evaluated using Aerobic Plate Count Technique. pH was measured using pH meter, turbidity was measured using nephelometer, temperature was measured using temperature probe and bottled water were considered chlorinated. Results: Among these 250 samples, the highest mean bacterial load was seen in public tap water samples (137×103 cfu/ml) and least mean bacterial load was seen on bottled water sample (28×103). Similarly, highest mean coliform load was seen on ground water samples (81 cfu/ml) and least mean coliform load was seen on bottled water samples (6 cfu/ml). Conclusion: This study has concluded that ground water samples contain large number of coliforms which suggest it might be fecally contaminated. Also the result had shown even bottled water contained coliforms. Therefore, it is recommended to treat ground water before using. Proper care must be taken during manufacture of bottled water.

scholarly journals PERSISTENCE OF TOTAL AND FECAL COLIFORMS AND Pseudomonas aeruginosa IN IN NATURA WATER SAMPLES FROM RIVERS THAT SUPPLY A WATER TREATMENT STATION IN CURITIBA, BRAZIL

2008 ◽  
Vol 30 (70/72) ◽  
Author(s):  
Mônica Paul Freitas ◽  
Marita Maciel Moreira Laskowski ◽  
Carlos Roberto Dalke ◽  
Patricia do Rocio Dalzoto ◽  
Ida Chapaval Pimentel
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Water Samples ◽  
Membrane Filtration ◽  
Survival Rates ◽  
Enteric Bacteria ◽  
Fecal Coliforms ◽  
Filtration Method ◽  
Total Coliforms ◽  
Water Treatment Station ◽  
Iguaçu River

An unknown parcel of the planet water is contaminated by chemical and/or biological agents. Human and other animals excreta have been associated to many infectious diseases that can be disseminated through contaminated water. Enteric bacteria and other pathogens can cause gastroenteritis, cholera, systemic infections, among others. Once in the environment, these microorganisms can persist for large periods of time and under certain conditions can even replicate, increasing their number. This paper aimed the research and evaluation of survival rates for total coliforms, fecal coliforms (Escherichia coli) and Pseudomonas aeruginosa in natura water samples from Passaúna and Iraí barrages and Iguaçú River, in Curitiba, Paraná. Colimetric analysis of water samples were performed by the membrane filtration method 64 Freitas MP, Blaskowski MMM, Dalke CR, Dalzoto PR, Pimentel IC. Estud and P. aeruginosa research was assayed using the multiple tubes method in Asparagin and Acetamyde Broth. Confirmatory tests such as catalase presence, citocrome oxidase test and pigment P (pyocyanin) production were also performed. It was observed the occurrence of total coliforms in water samples from Iguaçú River for approximately 50 days, while in Iraí and Passaúna barrages, the periods were 35 and 14 days, respectively. Fecal coliforms were found for about 35 days in samples from Iguaçú River and Iraí barrage, while in Passaúna barrage only for 14 days. P. aeruginosa persisted for approximately 63 days in all the water samples assayed. The higher persistence rate observed in P. aeruginosa can be related to an antagonism towards total and fecal coliforms, which had their populations decreased.

scholarly journals Microbiological Study on Supply Water and Treated Water in Dhaka City

1970 ◽  
Vol 1 (1) ◽  
pp. 42-45 ◽  
Author(s):  
Mrityunjoy Acharjee ◽  
Farjana Rahman ◽  
Sadia Afrin Beauty ◽  
Farahnaaz Feroz ◽  
M Majibur Rahman ◽  
...  
Keyword(s):  
Water Samples ◽  
Total Coliform ◽  
Faecal Coliform ◽  
Microbiological Analysis ◽  
Dhaka City ◽  
Sample Collection ◽  
Biochemical Tests ◽  
Salmonella Spp ◽  
Treated Water ◽  
Two Samples

Water borne diseases are the major cause of morbidity and mortality in developing countries like Bangladesh, where disease outbreaks occur due to unsafe drinking water, inadequate sanitation and poor hygienic practices. The objective of this study was to evaluate the quality of water used for drinking as well as household purposes in Dhaka city, Bangladesh. Eighteen water samples were collected from different rivers and canals. Among those, two samples were treated with chlorine. Physico-chemical parameters (pH, salinity, turbidity, conductivity and dissolved oxygen) were monitored during the time of sample collection using portable meters. For microbiological analysis, water samples were collected in sterile screw capped bottle. All Experiments were carried out within 24 hours after collecting the samples. All the water sources were found to be contaminated with total coliform, faecal coliform, Escherichia coli, Klebsiella spp., Salmonella spp., Shigella spp., Vibrio cholerae, Aeromonas spp. and Fungi except treated water samples. Several biochemical tests were performed for confirmation of suspected organisms. According to these results, huge amount of total coliform and faecal coliform were observed in every sample and these amount were very much similar for other organisms. Treated water samples were found to be microbiologically accepted than untreated water samples. DOI: http://dx.doi.org/10.3329/sjm.v1i1.9132    Stamford Journal of Microbiology, Vol.1(1), July 2011, p.42-45

scholarly journals Comparison of Microbiological and Physicochemical Quality of Tap and Dispenser Water from Different Mid-Range Restaurants of Dhaka City, Bangladesh

2016 ◽  
pp. 69-72 ◽  
Author(s):  
Nur Alam ◽  
Rubayat Jamal ◽  
SM Sabbir Alam ◽  
Mohammad Moniruzzaman
Keyword(s):  
Water Samples ◽  
Pathogenic Bacteria ◽  
Tap Water ◽  
Microbiological Quality ◽  
Total Coliform ◽  
Faecal Coliform ◽  
Quality Analysis ◽  
Health Concern ◽  
Plate Count ◽  
Physicochemical Quality

Dhaka is the most populous city in Bangladesh and the tenth-largest metropolitan in the world with about twelve million inhabitants. Most people of Dhaka have low or middle ranged incomes and frequently use roadside mid range restaurants to take their meals and drinking water. Water quality is a significant health concern in Bangladesh and is a probable source of many water borne diseases and outbreaks. Tap water and dispenser water samples were collected from ten roadside restaurants. These samples were analyzed for pH, salinity, conductivity, TDS (total dissolved solid) for physicochemical quality analysis while total coliform, faecal coliform and total heterotrophic bacterial counts were determined for microbiological quality analysis. All the samples conformed to the WHO standards of TDS, salinity and conductivity. Except for 20% of the tap water samples, pH of all the samples was found to be satisfactory. The heterotrophic plate count was in a range of 8.0 x 101 cfu/ml to 9.3 x 105 cfu/ml from tap water sample and 6.5 x 101 cfu/ml to TNTC from dispenser water samples. Only 10% of the tap water samples and 30% of the dispenser water samples complied with the WHO stipulated limit of total heterotrophic count (100 cfu/ml). In terms of total coliforms, all the tap water samples and 20% of the dispenser water samples were found to be contaminated, whereas 50% of both the tap water and dispenser water samples were contaminated with faecal coliforms, indicating possible faecal contamination and presence of pathogenic bacteria. By comparing coliform/faecal coliform count on tap and dispenser water it was found that dispenser water is safer than tap water.Bangladesh J Microbiol, Volume 31, Number 1-2,June-Dec 2014, pp 69-72

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