Separation of natural organic colloids with a PALL tangential flow filtration system

2009 ◽  
Vol 9 (5) ◽  
pp. 583-590 ◽  
Author(s):  
Berit Schwalger ◽  
Alejandro Spitzy
Keyword(s):  
Molecular Weight ◽  
Chemical Properties ◽  
Low Carbon ◽  
Size Fractionation ◽  
Membrane Cleaning ◽  
Tangential Flow Filtration ◽  
Tangential Flow ◽  
Filtration System ◽  
Physico Chemical ◽  
Flow Filtration

The applicability of a PALL tangential flow filtration (TFF) system for size fractionation of natural dissolved organic matter was investigated. The performance of polyethersulfone membranes with nominal molecular weight cut-off of 1 kDa, 5 kDa and 50 kDa was examined for isolation of low and high molecular weight compounds in fresh and estuarine waters with diverse physico-chemical properties. Detailed protocols for operating the TFF- system and for membrane cleaning are proposed. The ultrafiltration membranes can be efficiently cleaned to provide low carbon blanks (<0.09 mg/l). Standard colloid tests confirmed that the higher molecular weight compounds were isolated in the retentate and the lower molecular weight compounds remain in the permeate. Mass balance of fractionated natural samples showed good recoveries for dissolved organic carbon (DOC) (99±13% (1 kDa); 103±20% (5 kDa); 94±14% (50 kDa) (n=9). Moreover, high ionic strength or high DOC content did not enhance either fouling or contamination of the membrane. These findings demonstrate that the PALL TFF system is reliable for natural organic colloids fractionation in aquatic systems across both salinity and DOC gradients.

scholarly journals Model Validation and Process Design of Continuous Single Pass Tangential Flow Filtration Focusing on Continuous Bioprocessing for High Protein Concentrations

Processes ◽  
2019 ◽  
Vol 7 (11) ◽  
pp. 781 ◽  
Author(s):  
Maximilian Johannes Huter ◽  
Christoph Jensch ◽  
Jochen Strube
Keyword(s):  
Process Design ◽  
High Protein ◽  
Tangential Flow Filtration ◽  
Single Pass ◽  
Tangential Flow ◽  
Low Concentrations ◽  
Physico Chemical ◽  
Quality By Design Approach ◽  
Flow Filtration ◽  
Parameter Dependent

In this study, the continuous Single-Pass Tangential Flow Filtration (SPTFF) concept is adapted for high protein concentrations. The work is based on the previously validated physico-chemical model for low concentrations and high viscosities. The model contains the Stagnant Film Model for concentration polarization, as well as the Boundary Layer Model for the mass transfer through the membrane. The pressure drop is calculated as a function of the Reynolds number. By performing preliminary experiments with a single ultrafiltration (UF) cassette, the model parameter are determined. The presented model is validated for a multi-step Single-Pass Tangential Flow Filtration. With subsequent simulation studies, an optimized process is found and confirmed by experiments. The outcome of this work shows the potential to optimize this multi-parameter dependent unit operation. This is reached by a model-based optimization allowing significant reduction of experimental efforts and applying the Quality by Design approach consistently. Furthermore, a comparison between the experimental setup and a commercial module is examined.

Applicate Tangential Flow Filtration System to Improve Post CMP-Cleaning Performance

2021 ◽  
Vol MA2021-01 (20) ◽  
pp. 820-820
Author(s):  
Jaewon Lee ◽  
Hyeonmin Seo ◽  
Sang-hyeon Park ◽  
Seokjun Hong ◽  
Taesung Kim
Keyword(s):  
Tangential Flow Filtration ◽  
Cleaning Performance ◽  
Tangential Flow ◽  
Filtration System ◽  
Flow Filtration

scholarly journals Model-Based Design and Process Optimization of Continuous Single Pass Tangential Flow Filtration Focusing on Continuous Bioprocessing

Processes ◽  
2019 ◽  
Vol 7 (6) ◽  
pp. 317 ◽  
Author(s):  
Maximilian J. Huter ◽  
Jochen Strube
Keyword(s):  
Transmembrane Pressure ◽  
Tangential Flow Filtration ◽  
Single Pass ◽  
Tangential Flow ◽  
Model Based ◽  
Physico Chemical ◽  
Versatile Tool ◽  
Flow Filtration ◽  
Conceptual Process Design ◽  
Parameter Dependent

In this study the Single-Pass-Tangential-Flow-Filtration (SPTFF) concept for continuous ultrafiltration in bioprocessing is investigated. Based on a previously validated physico-chemical model for a single ultrafiltration cassette, the transfer to a multistage SPTFF is predicted and validated experimentally by concentration steps for bovine serum albumin (BSA) and the monoclonal antibody immunoglobulin G (IgG) are compared. The model applied for the ultrafiltration membrane contains the Stagnant Film Model (SFM) for concentration polarization, as well as the Osmotic Pressure Model (OPM) and the Boundary Layer Model (BLM) for the mass transfer through the membrane. In addition, pressure drop correlations as a function of the Reynolds number are included to describe the development of the transmembrane pressure over the length of the module. The outcome of this study shows the potential to improve this multi-parameter dependent unit operation by a model-based optimization allowing significant reduction of experimental efforts and applying the Quality by Design (QbD) approach consistently. Consequently, a versatile tool for conceptual process design is presented and further application is discussed.

scholarly journals Cyclic tangential flow filtration system for isolation of extracellular vesicles

2021 ◽  
Vol 5 (1) ◽  
pp. 016103
Author(s):  
Kimin Kim ◽  
Jungjae Park ◽  
Jik-Han Jung ◽  
Ruri Lee ◽  
Ji-Ho Park ◽  
...  
Keyword(s):  
Extracellular Vesicles ◽  
Tangential Flow Filtration ◽  
Tangential Flow ◽  
Filtration System ◽  
Flow Filtration

Physico-Chemical Properties of Recombinant Desulphatohirudin

1990 ◽  
Vol 63 (03) ◽  
pp. 499-504 ◽  
Author(s):  
A Electricwala ◽  
L Irons ◽  
R Wait ◽  
R J G Carr ◽  
R J Ling ◽  
...  
Keyword(s):  
Molecular Weight ◽  
Gel Filtration ◽  
Chemical Properties ◽  
Alpha Helix ◽  
Apparent Molecular Weight ◽  
Correlation Spectroscopy ◽  
Nmr Studies ◽  
Recombinant Hirudin ◽  
Physico Chemical ◽  
Recombinant Molecule

SummaryPhysico-chemical properties of recombinant desulphatohirudin expressed in yeast (CIBA GEIGY code No. CGP 39393) were reinvestigated. As previously reported for natural hirudin, the recombinant molecule exhibited abnormal behaviour by gel filtration with an apparent molecular weight greater than that based on the primary structure. However, molecular weight estimation by SDS gel electrophoresis, FAB-mass spectrometry and Photon Correlation Spectroscopy were in agreement with the theoretical molecular weight, with little suggestion of dimer or aggregate formation. Circular dichroism studies of the recombinant molecule show similar spectra at different pH values but are markedly different from that reported by Konno et al. (13) for a natural hirudin-variant. Our CD studies indicate the presence of about 60% beta sheet and the absence of alpha helix in the secondary structure of recombinant hirudin, in agreement with the conformation determined by NMR studies (17)

Concentration of live pico- and nanoplankton by means of tangential flow filtration

1989 ◽  
Vol 11 (6) ◽  
pp. 1213-1221 ◽  
Author(s):  
Klaus-Günther Barthel ◽  
Gerald Schneider ◽  
Rolf Gradinger ◽  
Jürgen Lenz
Keyword(s):  
Tangential Flow Filtration ◽  
Tangential Flow ◽  
Flow Filtration

High performance tangential flow filtration

1997 ◽  
Vol 56 (1) ◽  
pp. 71-82 ◽  
Author(s):  
Robert van Reis ◽  
Shishir Gadam ◽  
Leah N. Frautschy ◽  
Scott Orlando ◽  
Elizabeth M. Goodrich ◽  
...  
Keyword(s):  
High Performance ◽  
Tangential Flow Filtration ◽  
Tangential Flow ◽  
Flow Filtration
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