scholarly journals Cyclic tangential flow filtration system for isolation of extracellular vesicles

2021 ◽  
Vol 5 (1) ◽  
pp. 016103
Author(s):  
Kimin Kim ◽  
Jungjae Park ◽  
Jik-Han Jung ◽  
Ruri Lee ◽  
Ji-Ho Park ◽  
...  
Keyword(s):  
Extracellular Vesicles ◽  
Tangential Flow Filtration ◽  
Tangential Flow ◽  
Filtration System ◽  
Flow Filtration

scholarly journals Tangential Flow Filtration for Highly Efficient Concentration of Extracellular Vesicles from Large Volumes of Fluid

Cells ◽  
2018 ◽  
Vol 7 (12) ◽  
pp. 273 ◽  
Author(s):  
Sara Busatto ◽  
George Vilanilam ◽  
Taylor Ticer ◽  
Wen-Lang Lin ◽  
Dennis Dickson ◽  
...  
Keyword(s):  
Extracellular Vesicles ◽  
Culture Media ◽  
Biological Fluids ◽  
Physicochemical Characteristics ◽  
Cell Culture Media ◽  
Tangential Flow Filtration ◽  
Tangential Flow ◽  
Highly Efficient ◽  
Flow Filtration ◽  
Reproducible Manner

Concentration of extracellular vesicles (EVs) from biological fluids in a scalable and reproducible manner represents a major challenge. This study reports the use of tangential flow filtration (TFF) for the highly efficient isolation of EVs from large volumes of samples. When compared to ultracentrifugation (UC), which is the most widely used method to concentrate EVs, TFF is a more efficient, scalable, and gentler method. Comparative assessment of TFF and UC of conditioned cell culture media revealed that the former concentrates EVs of comparable physicochemical characteristics, but with higher yield, less single macromolecules and aggregates (<15 nm in size), and improved batch-to-batch consistency in half the processing time (1 h). The TFF protocol was then successfully implemented on fluids derived from patient lipoaspirate. EVs from adipose tissue are of high clinical relevance, as they are expected to mirror the regenerative properties of the parent cells.

Tangential flow filtration, a potential method for the scaled preparation of extracellular vesicles

Cytotherapy ◽  
2019 ◽  
Vol 21 (5) ◽  
pp. S57
Author(s):  
V. Börger ◽  
R. Dittrich ◽  
S. Staubach ◽  
S. Zumegen ◽  
P. Horn ◽  
...  
Keyword(s):  
Extracellular Vesicles ◽  
Potential Method ◽  
Tangential Flow Filtration ◽  
Tangential Flow ◽  
Flow Filtration

Applicate Tangential Flow Filtration System to Improve Post CMP-Cleaning Performance

2021 ◽  
Vol MA2021-01 (20) ◽  
pp. 820-820
Author(s):  
Jaewon Lee ◽  
Hyeonmin Seo ◽  
Sang-hyeon Park ◽  
Seokjun Hong ◽  
Taesung Kim
Keyword(s):  
Tangential Flow Filtration ◽  
Cleaning Performance ◽  
Tangential Flow ◽  
Filtration System ◽  
Flow Filtration

Separation of natural organic colloids with a PALL tangential flow filtration system

2009 ◽  
Vol 9 (5) ◽  
pp. 583-590 ◽  
Author(s):  
Berit Schwalger ◽  
Alejandro Spitzy
Keyword(s):  
Molecular Weight ◽  
Chemical Properties ◽  
Low Carbon ◽  
Size Fractionation ◽  
Membrane Cleaning ◽  
Tangential Flow Filtration ◽  
Tangential Flow ◽  
Filtration System ◽  
Physico Chemical ◽  
Flow Filtration

The applicability of a PALL tangential flow filtration (TFF) system for size fractionation of natural dissolved organic matter was investigated. The performance of polyethersulfone membranes with nominal molecular weight cut-off of 1 kDa, 5 kDa and 50 kDa was examined for isolation of low and high molecular weight compounds in fresh and estuarine waters with diverse physico-chemical properties. Detailed protocols for operating the TFF- system and for membrane cleaning are proposed. The ultrafiltration membranes can be efficiently cleaned to provide low carbon blanks (&lt;0.09 mg/l). Standard colloid tests confirmed that the higher molecular weight compounds were isolated in the retentate and the lower molecular weight compounds remain in the permeate. Mass balance of fractionated natural samples showed good recoveries for dissolved organic carbon (DOC) (99±13% (1 kDa); 103±20% (5 kDa); 94±14% (50 kDa) (n=9). Moreover, high ionic strength or high DOC content did not enhance either fouling or contamination of the membrane. These findings demonstrate that the PALL TFF system is reliable for natural organic colloids fractionation in aquatic systems across both salinity and DOC gradients.

Concentration of live pico- and nanoplankton by means of tangential flow filtration

1989 ◽  
Vol 11 (6) ◽  
pp. 1213-1221 ◽  
Author(s):  
Klaus-Günther Barthel ◽  
Gerald Schneider ◽  
Rolf Gradinger ◽  
Jürgen Lenz
Keyword(s):  
Tangential Flow Filtration ◽  
Tangential Flow ◽  
Flow Filtration

High performance tangential flow filtration

1997 ◽  
Vol 56 (1) ◽  
pp. 71-82 ◽  
Author(s):  
Robert van Reis ◽  
Shishir Gadam ◽  
Leah N. Frautschy ◽  
Scott Orlando ◽  
Elizabeth M. Goodrich ◽  
...  
Keyword(s):  
High Performance ◽  
Tangential Flow Filtration ◽  
Tangential Flow ◽  
Flow Filtration
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