scholarly journals Heterotrophic nitrification and aerobic denitrification using pure-culture bacteria for wastewater treatment

2018 ◽  
Vol 9 (1) ◽  
pp. 10-17 ◽  
Author(s):  
W. Khanichaidecha ◽  
A. Nakaruk ◽  
K. Ratananikom ◽  
R. Eamrat ◽  
F. Kazama
Keyword(s):  
Wastewater Treatment ◽  
Nitrogen Removal ◽  
Pure Culture ◽  
Water Reuse ◽  
Heterotrophic Nitrification ◽  
Aerobic Denitrification ◽  
Nitrification Rate ◽  
Wastewater Reclamation ◽  
Treatment Unit ◽  
Term Operation

Abstract Due to the high water demand and unsustainable water resource, wastewater reclamation and wastewater treatment prior to discharge have become current important issues. Various treatment technologies, such as biological processes, have been improved as alternatives. In this study, the biological nitrogen removal system using pure-culture Bacillus licheniformis was developed and used as an internal treatment unit in an aquarium to improve the effluent quality for water reuse. The efficiencies for NH4-N and total nitrogen (TN) removal and the quality of treated water verified the occurrence of heterotrophic nitrification and aerobic denitrification; the nitrification rate was 0.84 mg/L-h and the denitrification rate was 0.62 mg/L-h. The maximal NH4-N and TN removal efficiencies were approximately 73% at the influent NH4-N of 30 mg/L. However, the other competitive heterotroph of Pseudomonas sp. was observed, which resulted in dramatically decreasing efficiencies and an enlarged ratio of carbon consumption and nitrogen removal. Although the overall performance of the B. licheniformis system was lower than the system using mixed-culture nitrifying and heterotrophic denitrifying microorganisms, the advantages of the B. licheniformis system were ease of operation and the fact that it is a land-limited treatment system. The research is ongoing to enhance performance and maintain excellent efficiency in a long-term operation.

Microbiological conversions in nitrogen removal

1998 ◽  
Vol 38 (1) ◽  
pp. 1-7 ◽  
Author(s):  
M. C. M. Van Loosdrecht ◽  
M. S. M. Jetten
Keyword(s):  
Wastewater Treatment ◽  
Nitrogen Removal ◽  
Organic Substrate ◽  
Heterotrophic Nitrification ◽  
Aerobic Denitrification ◽  
Ammonium Oxidation ◽  
Treatment Processes ◽  
Nitrogen Conversion ◽  
The Many ◽  
Wastewater Treatment Systems

Nitrogen conversion processes are essential for most wastewater treatment systems. Due to the many possible conversions, and the complexity of analysing the reactions under actual conditions, there is much room for confusion. This review provides an overview of the possible microbiological nitrogen conversions described in literature. The relevance of these conversions with respect to wastewater treatment processes is discussed. Rates described for aerobic denitrification or denitrification by autotrophic nitrifiers are so low that these conversions probably do not play a significant role under practical conditions. Heterotrophic nitrification may be of relevance only when the wastewater contains a high COD/N ratio (>10). Anaerobic ammonium oxidation can occur in fully autotrophic systems with very long sludge retention times or biofilm systems. This conversion offers great opportunities since it allows us to denitrify with ammonium as electron donor, i.e. no organic substrate is needed in the nitrogen removal process.

scholarly journals Nitrogen Removal Characteristics of Pseudomonas putida Y-9 Capable of Heterotrophic Nitrification and Aerobic Denitrification at Low Temperature

2017 ◽  
Vol 2017 ◽  
pp. 1-7 ◽  
Author(s):  
Yi Xu ◽  
Tengxia He ◽  
Zhenlun Li ◽  
Qing Ye ◽  
Yanli Chen ◽  
...  
Keyword(s):  
Low Temperature ◽  
Pseudomonas Putida ◽  
Nitrogen Removal ◽  
Removal Rate ◽  
Heterotrophic Nitrification ◽  
Oxidation Products ◽  
Aerobic Denitrification ◽  
Nitrification Rate ◽  
High Concentration ◽  
Nitrate And Nitrite

The cold-adapted bacterium Pseudomonas putida Y-9 was investigated and exhibited excellent capability for nitrogen removal at 15°C. The strain capable of heterotrophic nitrification and aerobic denitrification could efficiently remove ammonium, nitrate, and nitrite at an average removal rate of 2.85 mg, 1.60 mg, and 1.83 mg NL−1 h−1, respectively. Strain Y-9 performed nitrification in preference to denitrification when ammonium and nitrate or ammonium and nitrite coexisted in the solution. Meantime, the presence of nitrate had no effect on the ammonium removal rate of strain Y-9, and yet the presence of high concentration of nitrite would inhibit the cell growth and decrease the nitrification rate. The experimental results indicate that P. putida Y-9 has potential application for the treatment of wastewater containing high concentrations of ammonium along with its oxidation products at low temperature.

scholarly journals Sewer Mining as a Distributed Intervention for Water-Energy-Materials in the Circular Economy Suitable for Dense Urban Environments: A Real World Demonstration in the City of Athens

Water ◽  
2021 ◽  
Vol 13 (19) ◽  
pp. 2764
Author(s):  
Argyro Plevri ◽  
Klio Monokrousou ◽  
Christos Makropoulos ◽  
Christos Lioumis ◽  
Nikolaos Tazes ◽  
...  
Keyword(s):  
Wastewater Treatment ◽  
Circular Economy ◽  
Water Reuse ◽  
Wastewater Treatment Plants ◽  
Treatment Plant ◽  
Urban Environments ◽  
Treated Wastewater ◽  
Aquifer Recharge ◽  
Treatment Unit ◽  
Plant Nursery

Water reuse and recycling is gaining momentum as a way to improve the circularity of cities, while recognizing the central role of water within a circular economy (CE) context. However, such interventions often depend on the location of wastewater treatment plants and the treatment technologies installed in their premises, while relying on an expensive piped network to ensure that treated wastewater gets transported from the treatment plant to the point of demand. Thus, the penetration level of treated wastewater as a source of non-potable supply in dense urban environments is limited. This paper focuses on the demonstration of a sewer mining (SM) unit as a source of treated wastewater, as part of a larger and more holistic configuration that examines all three ‘streams’ associated with water in CE: water, energy and materials. The application area is the Athens Plant Nursery, in the (water stressed) city of Athens, Greece. SM technology is in fact a mobile wastewater treatment unit in containers able to extract wastewater from local sewers, treat it directly and reuse at the point of demand even in urban environments with limited space. The unit consists of a membrane bioreactor unit (MBR) and a UV disinfection unit and produces high quality reclaimed water for irrigation and also for aquifer recharge during the winter. Furthermore, a short overview of the integrated nutrient and energy recovery subsystem is presented in order to conceptualise the holistic approach and circularity of the whole configuration. The SM technology demonstrates flexibility, scalability and replicability, which are important characteristics for innovation uptake within the emerging CE context and market.

scholarly journals Identification and Characterization of Janthinobacterium svalbardensis F19, a Novel Low-C/N-Tolerant Denitrifying Bacterium

2019 ◽  
Vol 9 (9) ◽  
pp. 1937 ◽  
Author(s):  
Yinyan Chen ◽  
Peng Jin ◽  
Zhiwen Cui ◽  
Tao Xu ◽  
Ruojin Zhao ◽  
...  
Keyword(s):  
Nitrogen Removal ◽  
Removal Rate ◽  
Pcr Amplification ◽  
Domestic Wastewater ◽  
Heterotrophic Nitrification ◽  
Aerobic Denitrification ◽  
Nitrite Accumulation ◽  
Specific Pcr ◽  
Domestic Wastewater Treatment ◽  
Initial Ph

Herein, we isolated Janthinobacterium svalbardensis F19 from sludge sediment. Strain F19 can simultaneously execute heterotrophic nitrification and aerobic denitrification under aerobic conditions. The organism exhibited efficient nitrogen removal at a C/N ratio of 2:1, with an average removal rate of 0.88 mg/L/h, without nitrite accumulation. At a C/N ratio of 2, an initial pH of 10.0, a culturing temperature of 25 °C, and sodium acetate as the carbon source, the removal efficiencies of ammonium, nitrate, nitrite, and hydroxylamine were 96.44%, 92.32%, 97.46%, and 96.69%, respectively. The maximum removal rates for domestic wastewater treatment for ammonia and total nitrogen were 98.22% and 92.49%, respectively. Gene-specific PCR amplification further confirmed the presence of napA, hao, and nirS genes, which may contribute to the heterotrophic nitrification and aerobic denitrification capacity of strain F19. These results indicate that this bacterium has potential for efficient nitrogen removal at low C/N ratios from domestic wastewater.

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