scholarly journals Identification and Characterization of Janthinobacterium svalbardensis F19, a Novel Low-C/N-Tolerant Denitrifying Bacterium

2019 ◽  
Vol 9 (9) ◽  
pp. 1937 ◽  
Author(s):  
Yinyan Chen ◽  
Peng Jin ◽  
Zhiwen Cui ◽  
Tao Xu ◽  
Ruojin Zhao ◽  
...  
Keyword(s):  
Nitrogen Removal ◽  
Removal Rate ◽  
Pcr Amplification ◽  
Domestic Wastewater ◽  
Heterotrophic Nitrification ◽  
Aerobic Denitrification ◽  
Nitrite Accumulation ◽  
Specific Pcr ◽  
Domestic Wastewater Treatment ◽  
Initial Ph

Herein, we isolated Janthinobacterium svalbardensis F19 from sludge sediment. Strain F19 can simultaneously execute heterotrophic nitrification and aerobic denitrification under aerobic conditions. The organism exhibited efficient nitrogen removal at a C/N ratio of 2:1, with an average removal rate of 0.88 mg/L/h, without nitrite accumulation. At a C/N ratio of 2, an initial pH of 10.0, a culturing temperature of 25 °C, and sodium acetate as the carbon source, the removal efficiencies of ammonium, nitrate, nitrite, and hydroxylamine were 96.44%, 92.32%, 97.46%, and 96.69%, respectively. The maximum removal rates for domestic wastewater treatment for ammonia and total nitrogen were 98.22% and 92.49%, respectively. Gene-specific PCR amplification further confirmed the presence of napA, hao, and nirS genes, which may contribute to the heterotrophic nitrification and aerobic denitrification capacity of strain F19. These results indicate that this bacterium has potential for efficient nitrogen removal at low C/N ratios from domestic wastewater.

scholarly journals Ammonium removal by a novel heterotrophic nitrifying and aerobic denitrifying bacterium Pseudomonas stutzeri KTB from wastewater

2015 ◽  
Vol 50 (3) ◽  
pp. 219-227 ◽  
Author(s):  
Maohong Zhou ◽  
Hairen Ye ◽  
Xiaowei Zhao
Keyword(s):  
Nitrogen Removal ◽  
Removal Rate ◽  
Pseudomonas Stutzeri ◽  
Culture Conditions ◽  
Aerobic Denitrification ◽  
Ammonium Concentration ◽  
Logarithmic Phase ◽  
Ammonium Removal ◽  
Removal Ratio ◽  
Initial Ph

The effects of culture conditions on a newly isolated Pseudomonas stutzeri KTB's ability to simultaneously perform heterotrophic nitrification and aerobic denitrification were investigated to determine its potential of application in nitrogen removal from wastewater. The results from experiments in the presence of 10 mmol/L of ammonium were as follows: succinate was the preferred carbon source, and the optimum C/N ratio, temperature, and initial pH were 10, 30 °C, and 7–8, respectively. Nitrogen removal took place not only in the logarithmic phase but also in the stationary phase. Under the optimum conditions, the nitrogen removal rate increased as the ammonium concentration elevated, until it was as high as 60 mmol/L. Meanwhile, the maximum specific growth rate decreased. The highest nitrogen removal rate of 0.977 mmol/L/h was observed at 60 mmol/L of ammonium and the maximum removal ratio of 85.6% at 40 mmol/L when the bacterial treatment for 48 h was completed. The strain was vulnerable to even higher ammonium loads. When incubated in anaerobically digested hennery wastewater containing 43.85 mmol/L of ammonium and 2.32 mmol/L of nitrate, the removal ratio and rate reached 82.4% and 0.397 mmol/L/h, respectively. The strain might be a great candidate for ammonium removal from wastewater.

Simultaneous organic stabilization and nitrogen removal in multi-stage biodrum system

2004 ◽  
Vol 50 (6) ◽  
pp. 95-101
Author(s):  
C. Chiemchaisri ◽  
C. Liamsangoun
Keyword(s):  
Wastewater Treatment ◽  
Nitrogen Removal ◽  
Rotational Speed ◽  
Removal Rate ◽  
Nitrogen Concentration ◽  
Domestic Wastewater ◽  
Organic Removal ◽  
Multi Stage ◽  
Domestic Wastewater Treatment ◽  
Removal Efficiencies

This paper presents the performance of a multi-stage biodrum system applied to domestic wastewater treatment. The organic stabilization and nitrogen removal efficiency in the system was investigated at different hydraulic retention times (HRT) of 12, 6 and 3 hours. The rotational speed of the biodrum was examined at 2,4 and 8 rpm. Average organic removal efficiencies in the system at different HRTs of 12, 6 and 3 hours were 96.3, 94.4 and 90.9%. Simultaneously, average nitrogen removal efficiencies were 91.5, 90.6 and 81.0%. The effect of rotational speed on nitrogen removal efficiencies in the system was clearly observed at a low HRT of 3 hours. The experimental results suggested that optimum HRT in the system was 6 hours. Moreover, they revealed that nitrogen removal efficiencies in the reactors operated at different rotational speed were in the same degree when considering the effluent nitrogen concentration. However, the reactors operated at lower rotational speed needed to employ higher numbers of biodrums (4 stages) than the others with higher rotational speed (3 and 2 stages at 4 and 8 rpm.) in order to achieve similar effluent qualities. At a rotational speed of 2 rpm, maximum nitrogen removal rate was found to be 0.2 kg/m3/d.

scholarly journals Nitrogen Removal Characteristics of Pseudomonas putida Y-9 Capable of Heterotrophic Nitrification and Aerobic Denitrification at Low Temperature

2017 ◽  
Vol 2017 ◽  
pp. 1-7 ◽  
Author(s):  
Yi Xu ◽  
Tengxia He ◽  
Zhenlun Li ◽  
Qing Ye ◽  
Yanli Chen ◽  
...  
Keyword(s):  
Low Temperature ◽  
Pseudomonas Putida ◽  
Nitrogen Removal ◽  
Removal Rate ◽  
Heterotrophic Nitrification ◽  
Oxidation Products ◽  
Aerobic Denitrification ◽  
Nitrification Rate ◽  
High Concentration ◽  
Nitrate And Nitrite

The cold-adapted bacterium Pseudomonas putida Y-9 was investigated and exhibited excellent capability for nitrogen removal at 15°C. The strain capable of heterotrophic nitrification and aerobic denitrification could efficiently remove ammonium, nitrate, and nitrite at an average removal rate of 2.85 mg, 1.60 mg, and 1.83 mg NL−1 h−1, respectively. Strain Y-9 performed nitrification in preference to denitrification when ammonium and nitrate or ammonium and nitrite coexisted in the solution. Meantime, the presence of nitrate had no effect on the ammonium removal rate of strain Y-9, and yet the presence of high concentration of nitrite would inhibit the cell growth and decrease the nitrification rate. The experimental results indicate that P. putida Y-9 has potential application for the treatment of wastewater containing high concentrations of ammonium along with its oxidation products at low temperature.

scholarly journals Biochar enhanced the nitrogen removal performance of heterotrophic nitrification-aerobic denitrification strain pseudomonas fluorescent sp. Z03 for insufficient carbon source wastewater under low temperature

2021 ◽  
Author(s):  
Jie Jiang ◽  
XiaoyanXu ◽  
Zhina Guo ◽  
Lianglun Sun ◽  
Meizhen Tang
Keyword(s):  
Electron Transfer ◽  
Low Temperature ◽  
Carbon Source ◽  
Nitrogen Removal ◽  
Removal Rate ◽  
Heterotrophic Nitrification ◽  
Redox Activity ◽  
Aerobic Denitrification ◽  
Control Group ◽  
N Removal

Abstract In this study, biochar BC400 and BC700 were prepared, characterized and coupled with heterotrophic nitrification-aerobic denitrification (HNAD) strain Z03 for nitrogen removal experiments. The characterization results showed that BC700 has a higher specific surface area and a more complex multilayered pore structure, with increased aromatic condensation and higher crystallinity. BC400 and BC700 both have good redox activity, while BC400 has stronger electron donor capacities and BC700 owns better electron transfer properties. In addition, both BC400 and BC700 contain relatively high levels of dissolved organic carbon (DOC), reaching at 62.95 and 51.617mg/g respectively. BC400/BC700 coupled with strain Z03 can significantly improve the NH4+-N removal performance of low-temperature and low C/N wastewater compared with the control group. At a dosage of 4.0 g/L, the removal rate of NH4+-N reached to 95.16% (BC400 + Z03) and 84.37% (BC700 + Z03) within 72h, respectively. Higher than the sum of adsorption by BC400/BC700 (16.19%/18.85%) and microbial degradation (41.03%). Besides, the BC400 + BC700 + Z03 NH4+-N removal systems provide higher nitrogen removal efficiencies than BC400/BC700 + Z03 nitrogen removal systems. When the dosage (BC400 + BC700, mass ratio 5:1) reaches 3.0g/L, it can achieve more than 90% NH4+-N removal rate within 48h. The reasons for the promotion of biochar on microbial denitrification were analyzed as follows: 1) DOC can provide an additional carbon source for microorganisms; 2) biochar, as a pH buffer, can neutralize the acidity due to nitrification; 3) BC400 and BC700, as materials with good redox activity, may play a role in promoting the activity of electron transfer system and enzyme activity.

scholarly journals In Situ Water Quality Improvement Mechanism (Nitrogen Removal) by Water-Lifting Aerators in a Drinking Water Reservoir

Water ◽  
2018 ◽  
Vol 10 (8) ◽  
pp. 1051 ◽  
Author(s):  
Zizhen Zhou ◽  
Tinglin Huang ◽  
Weijin Gong ◽  
Yang Li ◽  
Yue Liu ◽  
...  
Keyword(s):  
Drinking Water ◽  
Nitrogen Removal ◽  
High Throughput Sequencing ◽  
Removal Rate ◽  
Water Reservoir ◽  
Control Area ◽  
Nitrite Accumulation ◽  
Field Scale ◽  
Drinking Water Reservoir ◽  
N Removal

A field scale experiment was performed to explore the nitrogen removal performance of the water and surface sediment in a deep canyon-shaped drinking water reservoir by operating WLAs (water-lifting aerators). Nitrogen removal performance was achieved by increasing the densities and N-removal genes (nirK and nirS) of indigenous aerobic denitrifiers. After the operation of WLAs, the total nitrogen removal rate reached 29.1 ± 0.8% in the enhanced area. Ammonia and nitrate concentrations were reduced by 72.5 ± 2.5% and 40.5 ± 2.1%, respectively. No nitrite accumulation was observed. Biolog results showed improvement of carbon metabolism and carbon source utilization of microbes in the enhanced area. Miseq high-throughput sequencing indicated that the denitrifying bacteria percentage was also higher in the enhanced area than that in the control area. Microbial communities had changed between the enhanced and control areas. Thus, nitrogen removal through enhanced indigenous aerobic denitrifiers by the operation of WLAs was feasible and successful at the field scale.

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