The requirements and challenges of a mobile laboratory for onsite water microbiology assessment

2016 ◽  
Vol 11 (1) ◽  
pp. 198-209 ◽  
Author(s):  
Andrée F. Maheux ◽  
Luc Bissonnette ◽  
Vicky Huppé ◽  
Maurice Boissinot ◽  
Michel G. Bergeron ◽  
...  
Keyword(s):  
Drinking Water ◽  
Disease Outbreaks ◽  
Microbial Quality ◽  
Mobile Laboratory ◽  
Health Issues ◽  
Caribbean Islands ◽  
Molecular Microbiology ◽  
Adequate Evaluation ◽  
Decentralized Testing

Drinking water of good quality is essential to ensure the health and economical sustainability of human communities worldwide. The assessment of drinking water microbial quality is generally performed by detecting and/or quantifying faecal contamination indicators which may not provide an adequate evaluation of the health risks posed by several waterborne pathogens, for example Norovirus, Vibrio cholerae, and Cryptosporidium. In many instances, decentralized testing done in a mobile or more compact laboratory could increase the speed and capacity of predicting (or determining the source of) waterborne disease outbreaks, while offering unique opportunities to sensitize and train local populations on water and health issues. In this work, we describe the water molecular microbiology programme of the classical and molecular microbiology module of the Atlantis mobile laboratory complex, as well as the scientific, operational and design requirements that served to build a quite unique infrastructure used to study the microbial quality of drinking water in Northern Québec, Bermuda, and the Caribbean islands.

scholarly journals FAKTOR-FAKTOR YANG BERHUBUNGAN DENGAN KEJADIAN DIARE PADA ANAK USIA 4-6 TAHUN

2017 ◽  
Vol 3 (1) ◽  
pp. 28
Author(s):  
Selviana Selviana ◽  
Elly Trisnawati ◽  
Sitti Munawarah
Keyword(s):  
Drinking Water ◽  
Random Sampling ◽  
Water Storage ◽  
Sampling Technique ◽  
Microbial Quality ◽  
Community Based ◽  
Chi Square ◽  
Cross Sectional ◽  
Chi Square Test

Abstract: Factors Related To Occurrence Of Diarrhea In 4-6 Year Children. Diarrhea cases in 4-6-year-old children in Desa Kalimas raised annually, from 10% in 2013 to 33% in 2014, and 35% in 2015. This study aimed at factors related to diarrhea cases in 4-6-year-old children in Desa Kalimas Kecamatan Sungai Kakap. Cross Sectional approach was used forty one sample participated in this study. They were selected by using proportional random sampling technique. The data analysis using chi-square test with a 95% confidence level. The study revealed significant correlation of hand washing (p = 0,043 ; PR = 2,175), microbial quality of drinking water storage (p = 0,016 ; PR = 1,462), availability of family latrine (p = 0,003 ; PR = 3,242), and diarrhea cases. There was no significant correlation between diarrhea cases are the habit of boiling water with the microbial quality of drinking water, the source of water for washing cutlery, and microbial of drinking water, (p > 0,05). It is hoped health authorized staffs in Desa Kalimas apply community- based total sanitation, enhance clean and healthy behaviour, and socialize healthy drinking water storage based on Regulation of Minister of Healthy No. 3/2014.Abstrak: Faktor-Faktor Yang Berhubungan Dengan Kejadian Diare Pada Anak Usia 4-6 Tahun. Angka kejadian diare pada anak usia 4-6 tahun di Desa Kalimas mengalami peningkatan setiap tahunnya. Tahun 2013 kejadian diare sebesar 10%, tahun 2014 sebesar 33% dan 35% pada tahun 2015. Penelitian ini bertujuan untuk mengetahui faktor-faktor yang berhubungan dengan kejadian diare pada anak usia 4-6 tahun di Desa Kalimas Kecamatan Sungai Kakap. Penelitian ini menggunakan desain cross sectional dengan jumlah sampel  45 orang yang diambil menggunakan teknik proportional random sampling. Uji statistik yang digunakan uji chi-square dengan tingkat kepercayaan 95%. Hasil penelitian menunjukkan bahwa terdapat hubungan yang bermakna antara kebiasaan mencuci tangan dengan diare (p = 0,043; PR = 2,175), penyimpanan air minum dengan kualitas mikrobiologi air minum (p = 0,016; PR = 1,462), ketersediaan jamban keluarga dengan diare (p = 0,003; PR = 3,242). Variabel yang tidak berhubungan yaitu kebiasaan memasak air dengan kualitas mikrobiologi air minum, sumber air untuk mencuci alat makan/minum dan kualitas mikrobiologi air minum dengan diare (p ≥ 0,005). Disarankan agar di Desa Kalimas bisa diterapkan program STBM dan peningkatan penerapan PHBS pada masyarakat Desa Kalimas serta mensosialisasikan cara penyimpanan air minum yang baik sesuai dengan Permenkes No 3 tahun 2014.

Survey of Microbial Quality of Drinking Water in Rural Areas of Kashan-Iran in Second Half of 2008

2010 ◽  
Vol 14 (1) ◽  
pp. 59-63 ◽  
Author(s):  
M.B. Miranzadeh ◽  
M. Heidari ◽  
A.R. Mesdaghini ◽  
M. Younesian
Keyword(s):  
Drinking Water ◽  
Rural Areas ◽  
Microbial Quality

scholarly journals One-Year Surveillance of the Chemical and Microbial Quality of Drinking Water Shuttled to the Eolian Islands

Water ◽  
2014 ◽  
Vol 6 (1) ◽  
pp. 139-149 ◽  
Author(s):  
Antonio Proto ◽  
Ilaria Zarrella ◽  
Carmine Capacchione ◽  
Oriana Motta
Keyword(s):  
Drinking Water ◽  
Microbial Quality ◽  
One Year

scholarly journals Microbiological Quality of Kachumbari, a Raw Vegetable Salad Popularly Served Alongside Roast Meat in Kenya

2018 ◽  
Vol 2018 ◽  
pp. 1-7
Author(s):  
Kenneth M. Mbae ◽  
Mercy K. Ndwiga ◽  
Fredrick G. Kiruki
Keyword(s):  
Cross Section ◽  
Microbiological Quality ◽  
Disease Outbreaks ◽  
Human Consumption ◽  
Microbial Quality ◽  
Protection Agency ◽  
Indicator Microorganisms ◽  
E Coli ◽  
Food Borne

Raw salads are regularly implicated in food-borne disease outbreaks globally. Consumption of kachumbari, a raw vegetable salad, alongside roast meat is widespread in Kenya. The aim of this study was to evaluate the bacteriological quality of kachumbari samples (n=39) collected from a cross section of roasted meat eateries in Kenya. The United Kingdom’s Health Protection Agency guidelines were used to infer safety of the salads due to lack of local criteria for microbiological safety of ready-to-eat fresh produce placed in the market. Based on Escherichia coli counts, 14 (35.9%) of the samples were of satisfactory microbial quality (<20 CFU/g), 7 (17.9%) in the borderline (20–≤102 CFU/g), and 18 (46.2%) unsatisfactory (>102 CFU/g). All samples examined for staphylococci had counts falling within the borderline range (20–≤104 CFU/g). Collectively, 3 (7.7%) of the sampled salads were classified as potentially harmful to health and/or unfit for human consumption due to the presumptive presence of 2 (5.1%) Campylobacter spp. and 1 (2.6%) E. coli O157. Salmonella was not detected in any of the samples. The presence of hygiene indicator microorganisms and pathogens demonstrates that kachumbari salads present a public health risk.

scholarly journals Trace Elements Analysis in Drinking Water of Meghalaya by Using Graphite Furnace-Atomic Absorption Spectroscopy and in relation to Environmental and Health Issues

2014 ◽  
Vol 2014 ◽  
pp. 1-8 ◽  
Author(s):  
Elarina N. Dkhar ◽  
Paul S. Dkhar ◽  
Jasha Momo H. Anal
Keyword(s):  
Water Quality ◽  
Trace Elements ◽  
Drinking Water ◽  
Graphite Furnace ◽  
Major And Trace Elements ◽  
Point Of View ◽  
Health Issues ◽  
Trace Elements Analysis

Determination of the certain major and trace elements was carried out in drinking water supply scheme in three districts of Meghalaya. This work aims to identify trends resulting in the deterioration of drinking water which is also a potential source of environmental contaminants. About 50 samples, each from one district, were collected both from the source and various tanks and tap. The elements determined are Li, Na, Cr, Mn, Fe, Co, Ni, Cu, Zn, As, Rb, Ag, Au, Pb, Cd, Se, Ca, K, and Mg. The pH is slightly lower than neutral pH of 7 while the turbidity is very high even after treatment. The concentrations of Ca and Mg are found to be deficient. The elements Cr, Fe, Co, Ni, Zn, Mo, and Pb decrease after treatment while Mn, Cu, and Cd increase slightly after treatment. Se concentration is found to be much higher than expected. The results were compared with the standard recommendation values for the quality of drinking water. This study provides a general indication of where water-quality constituent concentrations met or exceeded water-quality standards and the data presented in this report will be useful from public health point of view.

MColiPAT kit for early detection of coliforms in water

2020 ◽  
Vol 20 (3) ◽  
pp. 871-877
Author(s):  
Ashish Tambi ◽  
Urmila Brighu ◽  
A. B. Gupta
Keyword(s):  
Water Quality ◽  
Drinking Water ◽  
Low Cost ◽  
Water Quality Assessment ◽  
Indicator Bacteria ◽  
Microbial Quality ◽  
Trained Personnel ◽  
Microbiological Water Quality ◽  
Rapid Tests

Abstract Determining the microbial quality of drinking water by assessing the presence/absence (P/A) or enumeration of indicator bacteria continues to be widely practiced worldwide. However, rapid tests are required for microbiological water quality assessment so that the information is available in the shortest possible time for initiating a timely intervention. Traditional methods for the enumeration of indicator bacteria are not only expensive but also need trained personnel. We have developed a low-cost kit, MColiPAT, and have validated its application for detection of coliforms in drinking water using the IDEXX Colilert-18 Quanti tray method. MColiPAT kit medium was able to detect coliforms down to a level of 3.1 MPN/100 ml within 10.5 hours. The sensitivity and specificity of the kit were 95.45% and 100% respectively. MColiPAT is found to be reliable and accurate for the detection of coliforms in drinking water.

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