MColiPAT kit for early detection of coliforms in water

2020 ◽  
Vol 20 (3) ◽  
pp. 871-877
Author(s):  
Ashish Tambi ◽  
Urmila Brighu ◽  
A. B. Gupta

Abstract Determining the microbial quality of drinking water by assessing the presence/absence (P/A) or enumeration of indicator bacteria continues to be widely practiced worldwide. However, rapid tests are required for microbiological water quality assessment so that the information is available in the shortest possible time for initiating a timely intervention. Traditional methods for the enumeration of indicator bacteria are not only expensive but also need trained personnel. We have developed a low-cost kit, MColiPAT, and have validated its application for detection of coliforms in drinking water using the IDEXX Colilert-18 Quanti tray method. MColiPAT kit medium was able to detect coliforms down to a level of 3.1 MPN/100 ml within 10.5 hours. The sensitivity and specificity of the kit were 95.45% and 100% respectively. MColiPAT is found to be reliable and accurate for the detection of coliforms in drinking water.

2019 ◽  
Vol 54 (2) ◽  
pp. 161-168
Author(s):  
G Yaqub ◽  
A Hamid ◽  
S Asghar

Rain is an effective way for removing pollutants from the atmosphere. The present study was initiated to determine rain water quality for its safe use as potable water, as well as a tool for indirect evaluation of air quality of different study areas. A total of 20 rain water samples were collected from areas including Kasur, Sheikhupura, Gujranwala and Lahore. The pH (5.49 + 0.323), turbidity (12.267 + 5.933NTU), Cl- (4785 + 1458.32ppm) and F- (16.44 + 4.52ppm) contents of samples are not in compliance with drinking water quality limits (Pakistan, WHO). Average sulphate (1.396 + 0.384 ppm) and NO3 − concentrations (52.35 + 12.11ppm) varied between 1.005-2.05ppm and 36.79-81.3ppm, respectively. Heavy metals analysis showed presence of Cu, Co, Mn and Zn concentrations below WHO limits while Cd and Pb concentrations exceeded WHO limits with values ranging between 0.005ppm-0.017ppm and 19ppm-254ppm, respectively . Findings indicate that rain water can provide an insight into the air quality of an area and its potential use as an alternative to drinking water, especially in areas of short domestic water supply. Bangladesh J. Sci. Ind. Res.54(2), 161-168, 2019


2019 ◽  
Vol 98 ◽  
pp. 09002
Author(s):  
Meriem Ameur ◽  
Fadoua Hamzaoui-Azaza ◽  
Moncef Gueddari

The Triassic aquifer is located in southereastern Tunisia, in the Medenine region, and being part of Jeffara's multilayer aquifer system. The aquifer is currently overexploited with an exploitation rate of 163%, which led to a generalized drop in the piezometric level in the order of 20 cm/year. This study applied conventional techniques such as hydrochemical compositions, multivariate statistical methods and Geographical Information Systems (GIS). To better identify the processes controlling the hydrogeochemical evolution of groundwater quality of Triassic aquifer and its suitability for drinking uses, 14 well water samples from the Triassic aquifer were analysed for physical character and chemical composition. In the groundwater, the respective order of cation and anion concentration was Na>Ca>Mg>K and SO4>Cl>HCO3. The chemical data of water samples from the study area presented by plotting on a Piper diagram reveals the predominance of two hydrochemical types: a Na-Ca-Mg-SO4 facies and a Na-Ca-Mg-Cl-SO4 facies. Analytical results demonstrate that the chemical composition of groundwater in Triassic aquifer is strongly influenced by residence time and flow path. Water quality deteriorates going from southwest to northeast across the region, coincident with the appearance a clay layer limiting the infiltration of rainwater. The majority of ions are above the maximum desirable limits recommended for drinking water by WHO guidelines and Tunisian Standards (NT.09.14). Based on the Water Quality Index (WQI), 36% of water samples of the Triassic aquifer are classified as "poor water" and they cannot be used for drinking purposes without prior treatment.


Author(s):  

The article is devoted to the analysis of the quality of surface water in Uzbekistan. Analyzed their impact on the living conditions and health of the population of the republic. The most difficult situation with drinking water supply and with the growth of diseases caused by the use of poor-quality drinking water falls on the Navoi, Khorezm, Surkhandarya regions and Karakalpakstan. The stated data makes it relevant to conduct research to determine the quality of surface waters and improve the methods of its assessment. The analysis of existing methods for assessing the quality of surface waters. The technique of assessment of the index of pollution of water applied now in Uzbekistan counted in compliance with “Methodical recommendations about the formalized complex evaluation test of the surface and ocean waters about hydro/chemical indexes” was developed in Soviet period and doesn’t meet the modern requirements. The need to improve the methods for assessing the quality of surface waters as compared with that currently used in Uzhydromet is indicated. One of the most promising at present is the “Method of integrated assessment of the degree of contamination of surface waters by hydro/chemical indicators” developed and used in operational practice in Russia. The most informative comprehensive estimat water pollution and the class of water quality. The author proposed methods for assessing the quality of surface waters, the calculation of which is based on the use of a larger amount of initial information and are more reliable than those used. In particular, the proposed methodology for assessing the quality of surface waters, taking into account the hazard class of ingredients.


Author(s):  
Resoketswe Charlotte Moropeng ◽  
Phumudzo Budeli ◽  
Maggy Ndombo Benteke Momba

This study assessed the impact of sanitation practices, hygienic and storage conditions on the quality of drinking water treated at point-of-use in Makwane Village. Subsequent to implementation of low-cost Household Water Treatment Devices which are the biosand filter with zeolite-silver (BSZ-SICG) and silver-impregnated porous pot (SIPP) filters in Makwane village, a structured questionnaire was designed to collect the following information: age of caretakers, number of children under the age of five, water storage conditions, sanitation amenities, and hygiene practices. Water quality from the sources to household level was assessed using culture-based and molecular techniques. The results revealed a significant association between the presence of Escherichia coli in treated drinking water with the age group of caregivers and the number of children ofless than the age of five [OR (95% CI) = 8.4737 (0.147–3.3497), p = 0.0141923 and OR (95% CI) = 9.1667 (0.1848–3.0159); p = 0.0165830, respectively]. Moreover, significant association was noted between hygiene practices (washing of hands with/without soap) and water quality in storage containers [OR (95% CI) = 16.000 (0.6763–3.9495), p = 0.0000125]. These findings further prove that there is still a dire need for reconsidering hygiene education in rural areas as the health benefits of water treated at point of use (POU) coupled with safe-storage condition interventions might not be guaranteed without proper hygiene. The results further highlighted the importance of washing hands in improving microbial quality of drinking water, which is the key factor for fighting against infectious diseases.


Author(s):  
Engr. Furqan wali

In this paper, the authors analyze the drinking water quality of Peshawar city due to which authors concluded that water quality assessment of 18 locations inside Peshawar of various union council. Groundwater samples were collected from a tube well and subjected to physical, chemical and biological analysis to check their suitability for the purpose of drinking. Results exposed that out of 18 samples 10 samples of water were found unfit for drinking purposes. In the 10, samples most of the effect on the water quality was from the chemical and biological contamination. It is concluded that the old defective supply system, infrastructure and storage, as well as their lack of maintenance are the reason behind the pollution of drinking water in Peshawar.


Author(s):  
V.E. Kriyt ◽  
Yu.N. Sladkova ◽  
O.V. Volchkova ◽  
V.V. Smirnov ◽  
V.Yu. Ananyev ◽  
...  

Drinking water of proper chemical and organoleptic quality, epidemically and radiologically safe and in sufficient quantity is one of the principal determinants of public health. Currently, the requirements for chemical water quality of the centralized drinking water supply in the Russian Federation are established by SanPiN 2.1.4.1074–01 and GN 2.1.5.1315–03. Despite the fact that both regulatory documents apply to drinking water, they contain different maximum permissible concentrations (MPC) of many chemicals. This ambiguity impedes the choice of standard values and leads to inconsistencies in drinking water quality assessment. Both national and foreign water quality standards have a single regulatory principle but the number and list of regulated chemicals and their standard values in different countries vary considerably. It is essential to develop hygienic standards that combine national and international experience in regulating drinking water quality. We compared Russian hygienic standards for chemical drinking water contaminants with appropriate foreign standards in order to resolve the issue of the necessity and possibility of their optimization.


2021 ◽  
Vol 909 (1) ◽  
pp. 012001
Author(s):  
I A Putri ◽  
F Ansari

Abstract Pattunuang asue Nature Tourism Object is a primary destination at Bantimurung Bulusaraung National Park. Visitors of Pattunuang asue used the Pattunuang river to conduct their activities, such as swimming, bathing, river tubing, and also used the river water for drinking and cooking. However, using river water for these activities needs to fulfill standard water quality for tourism activities and drinking water. Therefore, this study aims to determine the quality of the Pattunuang river water. Data of physical parameters and conditions of river water were conducted using the survey method. We also collected water samples using the purposive sampling method. Furthermore, these samples were analyzed at the laboratory to measure other physical data such as total suspended solids (TSS) and total dispended solids (TDS), chemical, and microbiology. Data were analyzed following water quality parameters, compared to several regulations regarding water quality standards for tourism and drinking water. The results showed that the Pattunuang river water fulfills most of the standard of physical quality for tourism and drinking water, except TDS. However, some chemical parameters exceeded the threshold for tourism and drinking water. Microbiology parameters meet the standard requirements for several regulations but did not meet the requirements for water that can be drunk directly. Furthermore, this study discussed how to overcome this problem.


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