A ZnO–CNT nanocomposite based electrochemical DNA biosensor for meningitis detection

RSC Advances ◽  
2016 ◽  
Vol 6 (80) ◽  
pp. 76214-76222 ◽  
Author(s):  
Manvi Tak ◽  
Vinay Gupta ◽  
Monika Tomar
Keyword(s):  
Dna Detection ◽  
High Sensitivity ◽  
Dna Biosensor ◽  
Electrochemical Dna Biosensor

The article focuses on the synthesis of ZnO and CNTs based electrochemical DNA biosensor and its application towards meningitis DNA detection with high sensitivity as well as selectivity.

scholarly journals Application of Gold Nanoparticles for Electrochemical DNA Biosensor

2014 ◽  
Vol 2014 ◽  
pp. 1-7 ◽  
Author(s):  
Ahmed Mishaal Mohammed ◽  
Ruslinda A. Rahim ◽  
Ibraheem Jaleel Ibraheem ◽  
Foo Kai Loong ◽  
Hasrul Hisham ◽  
...  
Keyword(s):  
Thin Films ◽  
Gold Nanoparticles ◽  
Dna Hybridization ◽  
Dna Detection ◽  
Dna Biosensor ◽  
Base Line ◽  
Frequency Range ◽  
Dna Immobilization ◽  
Electrochemical Dna Biosensor ◽  
Chemically Modified

An electrochemical DNA biosensor was successfully fabricated by using (3-aminopropyl)triethoxysilane (APTES) as a linker molecule combined with the gold nanoparticles (GNPs) on thermally oxidized SiO2thin films. The SiO2thin films surface was chemically modified with a mixture of APTES and GNPs for DNA detection in different time periods of 30 min, 1 hour, 2 hours, and 4 hours, respectively. The DNA immobilization and hybridization were conducted by measuring the differences of the capacitance value within the frequency range of 1 Hz to 1 MHz. The capacitance values for DNA immobilization were 160 μF, 77.8 μF, 70 μF, and 64.6 μF, respectively, with the period of time from 30 min to 4 hours. Meanwhile the capacitance values for DNA hybridization were 44 μF, 54 μF, 55 μF, and 61.5 μF, respectively. The capacitance value of bare SiO2thin film was 0.42 μF, which was set as a base line for a reference in DNA detection. The differences of the capacitance value between the DNA immobilization and hybridization revealed that the modified SiO2thin films using APTES and GNPs were successfully developed for DNA detection.

scholarly journals An electrochemical DNA biosensor for trace amounts of mercury ion quantification

2016 ◽  
Vol 14 (5) ◽  
pp. 808-815 ◽  
Author(s):  
Ferdaous Maâtouk ◽  
Mouna Maâtouk ◽  
Karima Bekir ◽  
Houcine Barhoumi ◽  
Abderrazak Maaref ◽  
...  
Keyword(s):  
Electrochemical Impedance ◽  
Specific Interaction ◽  
High Sensitivity ◽  
Differential Pulse ◽  
Mercury Ion ◽  
Dna Biosensor ◽  
Electrochemical Dna Biosensor ◽  
Dna Oligonucleotides ◽  
Wastewater Samples ◽  
Eis Measurements

In this work we report the development of an electrochemical DNA biosensor with high sensitivity for mercury ion detection. A new matrix based on gold nanoparticles (AuNPs)-glutathione (GSH)/cysteine was investigated. The interaction between DNA oligonucleotides and Hg2+ ions followed by the formation of Thymine–Hg2+–Thymine (T–Hg2+–T) structures was quantified using different electrochemical methods. It has been shown that the electrochemical impedance spectroscopy (EIS) measurements and the differential pulse voltammetry (DPV) confirmed the specific interaction between the oligonucleotide receptor layer and the Hg2+ ions. Besides, the developed sensor exhibited high sensitivity towards mercury among some examined metal ions such as Pb2+, Cu2+ and Cd2+. As a result, a high electrochemical response and low detection limit of 50 pM were estimated in the case of Hg2+ ions. The developed DNA biosensor was applied successfully to the determination of Hg2+ions in wastewater samples.

A Sequence‐Selective Electrochemical DNA Biosensor Based on HRP‐Labeled Probe for Colorectal Cancer DNA Detection

2008 ◽  
Vol 41 (1) ◽  
pp. 24-35 ◽  
Author(s):  
Zhijie Wang ◽  
Yunhui Yang ◽  
Kailiang Leng ◽  
Jishan Li ◽  
Fan Zheng ◽  
...  
Keyword(s):  
Colorectal Cancer ◽  
Dna Detection ◽  
Dna Biosensor ◽  
Electrochemical Dna Biosensor ◽  
Labeled Probe

scholarly journals Electrochemical DNA biosensor for the multianalyte detection of HPV-16 and HPV-18 in oral and cervical lesions

2022 ◽  
Author(s):  
Thanyarat Chaibun ◽  
Patcharanin Thanasapburachot ◽  
Patutong Chatchawal ◽  
Su Yin Lee ◽  
Sirimanas Jiaranuchart ◽  
...  
Keyword(s):  
High Sensitivity ◽  
Dna Biosensor ◽  
Cervical Lesions ◽  
Hpv 16 ◽  
Electrochemical Dna Biosensor ◽  
Cervical Cancers ◽  
Sandwich Hybridization ◽  
Reporter Probe ◽  
Multianalyte Detection ◽  
Hpv 18

Abstract Infection with high-risk human papillomavirus (HPV) is a major risk factor for oral and cervical cancers. In this study, we developed an electrochemical DNA biosensor for detection of HPV-16 and HPV-18, which are the 2 most prevalent genotypes for development of oral and cervical cancers. The assay involves the sandwich hybridization of the HPV target to silica-redox dye reporter probe and capture probe, followed by electrochemical detection. The sensor was found to be highly specific and sensitive, with detection limit of 22 fM for HPV-16 and 20 fM for HPV-18, between the range of 1 fM to 1 µM. Evaluation with oral and cervical samples showed that the biosensor result was consistent with the nested PCR /gel electrophoresis detection. The biosensor assay could be completed within 90 minutes. Due to its simplicity, rapidity and high sensitivity, this biosensor could be used as an alternative method for HPV detection in clinical laboratories. [151 words]

Electrochemical DNA biosensor for the detection of specific gene related to Microcystis species

2001 ◽  
Vol 3 (5) ◽  
pp. 224-228 ◽  
Author(s):  
Fei Yan ◽  
Arzum Erdem ◽  
Burcu Meric ◽  
Kagan Kerman ◽  
Mehmet Ozsoz ◽  
...  
Keyword(s):  
Dna Biosensor ◽  
Specific Gene ◽  
Electrochemical Dna Biosensor
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