scholarly journals Detection of assemblages A and B of Giardia duodenalis in water and sewage from São Paulo state, Brazil

2011 ◽  
Vol 9 (2) ◽  
pp. 361-367 ◽  
Author(s):  
Licia Natal Fernandes ◽  
Patrícia Pereira de Souza ◽  
Ronalda Silva de Araújo ◽  
Maria Tereza Pepe Razzolini ◽  
Rodrigo Martins Soares ◽  
...  
Keyword(s):  
Public Health ◽  
Phylogenetic Analysis ◽  
Water Samples ◽  
Nested Pcr ◽  
Giardia Duodenalis ◽  
Spring Water ◽  
Human Faeces ◽  
Paulo State ◽  
Treated Sewage ◽  
Assemblage B

Giardia duodenalis is a protozoan that parasitizes humans and other mammals and causes giardiasis. Although its isolates have been divided into seven assemblages, named A to G, only A and B have been detected in human faeces. Assemblage A isolates are commonly divided into two genotypes, AI and AII. Even though information about the presence of this protozoan in water and sewage is available in Brazil, it is important to verify the distribution of different assemblages that might be present, which can only be done by genotyping techniques. A total of 24 raw and treated sewage, surface and spring water samples were collected, concentrated and purified. DNA was extracted, and a nested PCR was used to amplify an 890 bp fragment of the gdh gene of G. duodenalis, which codes for glutamate dehydrogenase. Positive samples were cloned and sequenced. Ten out of 24 (41.6%) samples were confirmed to be positive for G. duodenalis by sequencing. Phylogenetic analysis grouped most sequences with G. duodenalis genotype AII from GenBank. Only two raw sewage samples presented sequences assigned to assemblage B. In one of these samples genotype AII was also detected. As these assemblages/genotypes are commonly associated to human giardiasis, the contact with these matrices represents risk for public health.

scholarly journals Radon Activity Concentrations in Natural Hot Spring Water: Dose Assessment and Health Perspective

2021 ◽  
Vol 18 (3) ◽  
pp. 920
Author(s):  
Eka Djatnika Nugraha ◽  
Masahiro Hosoda ◽  
June Mellawati ◽  
Untara Untara ◽  
Ilsa Rosianna ◽  
...  
Keyword(s):  
Public Health ◽  
Effective Dose ◽  
Radiation Protection ◽  
Water Samples ◽  
Hot Springs ◽  
Hot Spring ◽  
Annual Effective Dose ◽  
Spring Water ◽  
Dose Assessment ◽  
West Java

The world community has long used natural hot springs for tourist and medicinal purposes. In Indonesia, the province of West Java, which is naturally surrounded by volcanoes, is the main destination for hot spring tourism. This paper is the first report on radon measurements in tourism natural hot spring water in Indonesia as part of radiation protection for public health. The purpose of this paper is to study the contribution of radon doses from natural hot spring water and thereby facilitate radiation protection for public health. A total of 18 water samples were measured with an electrostatic collection type radon monitor (RAD7, Durridge Co., USA). The concentration of radon in natural hot spring water samples in the West Java region, Indonesia ranges from 0.26 to 31 Bq L−1. An estimate of the annual effective dose in the natural hot spring water area ranges from 0.51 to 0.71 mSv with a mean of 0.60 mSv for workers. Meanwhile, the annual effective dose for the public ranges from 0.10 to 0.14 mSv with an average of 0.12 mSv. This value is within the range of the average committed effective dose from inhalation and terrestrial radiation for the general public, 1.7 mSv annually.

scholarly journals Occurrence and Multi-Locus Analysis of Giardia duodenalis in Coypus (Myocastor coypus) in China

Pathogens ◽  
2021 ◽  
Vol 10 (2) ◽  
pp. 179
Author(s):  
Zhaohui Cui ◽  
Deguo Wang ◽  
Wen Wang ◽  
Ying Zhang ◽  
Bo Jing ◽  
...  
Keyword(s):  
Phylogenetic Analysis ◽  
Genetic Variation ◽  
Giardia Duodenalis ◽  
Nucleotide Sequencing ◽  
The Novel ◽  
Gastrointestinal Parasite ◽  
Myocastor Coypus ◽  
Pcr Screening ◽  
Assemblage B

Giardia duodenalis is a major gastrointestinal parasite found globally in both humans and animals. This work examined the occurrence of G. duodenalis in coypus (Myocastor coypus) in China. Multi-locus analysis was conducted to evaluate the level of genetic variation and the potential zoonotic role of the isolates. In total, 308 fecal samples were collected from seven farms in China and subjected to PCR screening to reveal G. duodenalis. Notably, G. duodenalis was detected in 38 (12.3%) specimens from assemblages A (n = 2) and B (n = 36). Positive samples were further characterized by PCR and nucleotide sequencing of the triose phosphate isomerase (tpi), beta giardin (bg), and glutamate dehydrogenase (gdh) genes. Multi-locus genotyping yielded 10 novel multi-locus genotypes (MLGs) (one MLG and nine MLGs for assemblages A and B, respectively). Based on the generated phylogenetic tree, AI–novel 1 clustered more closely with MLG AI-2. Furthermore, within the assemblage B phylogenetic analysis, the novel assemblage B MLGs were identified as BIV and clustered in the MLG BIV branch. This is the first report of G. duodenalis in coypus in China. The presence of zoonotic genotypes and subtypes of G. duodenalis in coypus suggests that these animals can transmit human giardiasis.

scholarly journals Genetic diversity of Giardia isolates from patients in Chandigarh region: India

2021 ◽  
Vol 14 (1) ◽  
Author(s):  
Shabnam Thakur ◽  
Upninder Kaur ◽  
Rakesh Sehgal
Keyword(s):  
Genetic Diversity ◽  
Phylogenetic Analysis ◽  
Glutamate Dehydrogenase ◽  
Nested Pcr ◽  
Epidemiological Studies ◽  
Nucleotide Sequences ◽  
Neighbor Joining ◽  
Pcr Targeting ◽  
Assemblage B

Abstract Objective The aim of study was to characterize Giardia isolates genetically among patients in Chandigarh region, India. For this, nested PCR targeting fragment of the glutamate dehydrogenase (GLUD1 earlier named as GDH) gene was used. Phylogenetic analysis was done by constructing neighbor-joining tree made out of the nucleotide sequences of G. intestinalis isolates obtained in this study and with the known sequences published in GenBank. Results Out of 40 samples, GLUD1 gene was amplified in 33 samples (82.5%). The product of GLUD1 gene was successfully sequenced only in 32 samples. In these samples, assemblage B was found in 27 (84.37%) samples whereas 5 (15.6%) samples had assemblage A. Among assemblage B most of them were of BIII. Therefore, genotyping of Giardia would be helpful in conducting epidemiological studies.

scholarly journals Sequencing and phylogenetic analysis of the stn gene of Salmonella species isolated from different environmental sources at Lake Qarun protectorate: The role of migratory birds and public health importance

2021 ◽  
pp. 2764-2772
Author(s):  
Hanan S. Khalefa ◽  
Zeinab S. Ahmed ◽  
Fatma Abdel-Kader ◽  
Eman M. Ismail ◽  
Esraa A. Elshafiee
Keyword(s):  
Public Health ◽  
Phylogenetic Analysis ◽  
Water Samples ◽  
Brine Shrimp ◽  
Chicken Manure ◽  
Farm Animals ◽  
Aquatic Bird ◽  
Aquatic Birds ◽  
Fulica Atra ◽  
Lake Qarun

Background and Aim: Salmonella causes most foodborne bacterial illnesses worldwide. It is found in various hosts, including pets, farm animals, and wild animals, as well as the environment. This study aimed to examine the epidemiological relationship between Salmonella isolates from aquatic environments and those from other avian hosts. Materials and Methods: The study examined 12 water samples, 210 aquatic animals, and 45 migratory aquatic bird samples collected from the protected area of Lake Qarun in El-Fayoum Governorate, Egypt, during migration seasons from different waterfowl migration areas (from October 2018 to January 2019). In addition, 45 fecal samples from domestic chickens were collected from the same geographic location from poultry farms. Bacteriological examination and polymerase chain reaction assay of two virulence genes (i.e., invA and stn) were performed to isolate and identify Salmonella. Results: Salmonella was isolated from 58.3% (7/12) of Lake Qarun water samples, 13.3% (6/45) of migratory waterfowl, 6.6% of (3/45) of chickens (Gallus gallus domesticus), and 4.3% (3/70) of fish and pooled brine shrimp. In migratory aquatic bird species that were sampled, Salmonella were isolated from 23.1% (3/13) of Eurasian coot (Fulica atra), 12.5%, (1/8) of green-winged teal (Anas cardolinesis), 10% (2/20) of northern shoveler (Spatula clypeata), and 0% (0/4) of mallard duck (Anas platyrhynchos). In 35 Tilapia, Salmonella was isolated by (8.6%) 5.7% of external surfaces, 2.85% from the intestine, and 0% from the muscle. No Salmonella was isolated from the 175 brine shrimp samples. Phylogenetic analysis using the stn genes of Salmonella isolated from the aquatic environment, migratory aquatic birds, and chicken showed a strong association between these isolates. In addition, a higher nucleotide identity percentage was observed between the sequences recovered from migratory aquatic birds and Lake Qarun water samples. Conclusion: Salmonella distribution was confirmed through migratory aquatic birds, based on our phylogeny tree analysis, Salmonella considered a likely carrier of zoonotic bacterial pathogens. Furthermore, the close relationship between chicken and fish sequences highlights the scenarios of using chicken manure in fish farms and its public health implications. The presence of Salmonella in different environmental sources spotlights the urgent need to control and break down its epidemiological cycle.

scholarly journals Molecular Characterization of Giardia duodenalis in Children and Adults Sampled in Algeria

2020 ◽  
Vol 9 (1) ◽  
pp. 54
Author(s):  
Salem Belkessa ◽  
Daniel Thomas-Lopez ◽  
Karim Houali ◽  
Farida Ghalmi ◽  
Christen Rune Stensvold
Keyword(s):  
Real Time ◽  
Molecular Characterization ◽  
Real Time Pcr ◽  
Intestinal Parasites ◽  
Giardia Duodenalis ◽  
Specific Pcr ◽  
Stool Samples ◽  
Pcr Targeting ◽  
Assemblage B

The molecular epidemiology of giardiasis in Africa remains unclear. A study was carried out across four hospitals in Algeria. A total of 119 fecal samples from 55 children, 37 adults, and 27 individuals of undetermined age, all scored positive for intestinal parasites by microscopy, and were screened by real-time PCR for Giardia. Molecular characterization of Giardia was performed by assemblage-specific PCR and PCR targeting the triose phosphate isomerase gene (tpi). Of the 119 samples, 80 (67%) were Giardia-positive by real-time PCR. For 48 moderately-highly real-time PCR-positive samples, tpi genotyping assigned 22 samples to Assemblage A and 26 to Assemblage B. Contrary to Assemblage A, Assemblage B exhibited substantial genetic diversity and allelic heterozygosity. Assemblage-specific PCR proved to be specific for discriminating Assemblage A or B but not as sensitive as tpi genotyping. We confirmed that real-time PCR is more sensitive than microscopy for detecting Giardia in stool samples and that robust amplification and sequencing of the tpi gene is feasible when moderate-to-strongly real-time PCR-positive samples are used. This study is one of the few performed in Africa providing genotyping data on Giardia infections in humans. Both assemblages A and B were commonly seen and not associated with specific sociodemographic data.

scholarly journals Evolution of bacterial meningitis diagnosis in Sao Paulo State-Brazil and future challenges

2013 ◽  
Vol 71 (9B) ◽  
pp. 672-676 ◽  
Author(s):  
Maristela Marques Salgado ◽  
Maria Gisele Goncalves ◽  
Lucila Okuyama Fukasawa ◽  
Fabio Takenori Higa ◽  
Juliana Thalita Paulino ◽  
...  
Keyword(s):  
Public Health ◽  
Bacterial Meningitis ◽  
Public Health Problem ◽  
Sao Paulo ◽  
Immunological Methods ◽  
Haemophilus Influenzae Type ◽  
Molecular Technique ◽  
São Paulo ◽  
Paulo State ◽  
São Paulo State

Bacterial meningitis (BM) is a severe disease and still represents a serious public health problem with high rates of morbidity and mortality. The most common cases of BM around the world, mainly in Brazil, have been caused by Neisseria meningitidis, Streptococcus pneumoniae, and Haemophilus influenzae type b. Bacterial culture is the gold-standard technique for BM confirmation, but approximately 50% of suspected cases are not culture-confirmed, due to problems related to improper transportation and seeding or previous antibiotic treatment. Immunological methods present low sensitivity and have possibility of cross-reactions. Real time PCR (qPCR) is a molecular technique and has been successful used for BM diagnosis at Instituto Adolfo Lutz in São Paulo State, Brazil, since 2007. The incorporation of qPCR in the Public Health surveillance routine in our state resulted in diminishing 50% of undetermined BM cases. Our efforts are focused on qPCR implementation in the BM diagnostic routine throughout Brazil.

scholarly journals South-east Asian Zika virus strain linked to cluster of cases in Singapore, August 2016

2016 ◽  
Vol 21 (38) ◽  
Author(s):  
Sebastian Maurer-Stroh ◽  
Tze-Minn Mak ◽  
Yi-Kai Ng ◽  
Shiau-Pheng Phuah ◽  
Roland G Huber ◽  
...  
Keyword(s):  
Public Health ◽  
Phylogenetic Analysis ◽  
Virus Strain ◽  
Zika Virus ◽  
East Asian ◽  
Public Health Emergency ◽  
Global Public Health ◽  
Genome Sequences ◽  
First Case ◽  
Large Outbreak

Zika virus (ZIKV) is an ongoing global public health emergency with 70 countries and territories reporting evidence of ZIKV transmission since 2015. On 27 August 2016, Singapore reported its first case of local ZIKV transmission and identified an ongoing cluster. Here, we report the genome sequences of ZIKV strains from two cases and find through phylogenetic analysis that these strains form an earlier branch distinct from the recent large outbreak in the Americas.

Nested PCR targeting intergenic spacer (IGS) in genotyping of Giardia duodenalis isolated from symptomatic and asymptomatic infected Egyptian school children

2016 ◽  
Vol 116 (2) ◽  
pp. 763-771 ◽  
Author(s):  
Eman M. Hussein ◽  
Ola A. Ismail ◽  
Amira B. Mokhtar ◽  
Samer E. Mohamed ◽  
Rania M. Saad
Keyword(s):  
School Children ◽  
Nested Pcr ◽  
Giardia Duodenalis ◽  
Intergenic Spacer ◽  
Pcr Targeting

scholarly journals Investigation of Cryptosporidium spp. and Giardia spp. in Lake Eğirdir (Isparta)

2021 ◽  
Author(s):  
Tuğba Sağlam ◽  
Serdar Düşen ◽  
Meral Apaydın Yağcı ◽  
Abdülkadir Yağcı
Keyword(s):  
Public Health ◽  
Drinking Water ◽  
Water Samples ◽  
Average Density ◽  
Agricultural Irrigation ◽  
Control Programs ◽  
Cryptosporidium Spp ◽  
Direct Microscopic Examination ◽  
Different Seasons ◽  
Autumn And Winter

Objective: The aim of this study was to assess both the presence and seasonal variability of Cryptosporidium spp. and Giardia spp. in Eğirdir Lake within the borders of Isparta province, which is used for drinking, agricultural irrigation and recreational purposes. Method: The research was carried out between July 2016 and January 2017 and water samples were taken from five different stations in three different seasons in Lake Eğirdir. After direct microscopic examination of the samples (Native-Lugol method), they were stained with Modified Acid Fast (MAF), and examined under the light microscope for parasites. Results: Cryptosporidium spp and Giardia spp were detected in 15 water samples in summer months, with an average density of 99.2% and 93.3% respectively, in Lake Eğirdir. In addition, both parasites were also detected intensively in autumn and winter Conclusion: The use of Lake Eğirdir for daily needs of people, agriculture andrecreational purposes cause increase in protozoal density. Thus, it is necessary to conduct parasitological studies on Lake Eğirdir, especially during the periods of swimming tourism, to determine the protozoal epidemiology in humans and animals. In addition, it is important to carry out adequate disinfection processes and plan the necessary control programs in terms of public health in the regions where Lake Eğirdir is used as drinking water.

scholarly journals Toxocara cati and other parasitic enteropathogens: more commonly found in owned cats with gastrointestinal signs than in clinically healthy ones

2021 ◽  
Author(s):  
Aurora L. Ursache ◽  
Adriana Györke ◽  
Viorica Mircean ◽  
Mirabela Oana Dumitrache ◽  
Andrei Răzvan Codea ◽  
...  
Keyword(s):  
Public Health ◽  
Risk Factors ◽  
Giardia Duodenalis ◽  
Gastrointestinal Parasites ◽  
Parasitic Infections ◽  
Toxocara Cati ◽  
The Public ◽  
Health And Wellbeing ◽  
Faecal Samples ◽  
Flotation Method

Abstract Gastrointestinal parasites are involved in the health and wellbeing of cats and some of them, due to their zoonotic character represent a problem for the public health. This study aimed to assess the prevalence of parasitic infections in cats with digestive symptoms. Faecal samples collected from 137 cats were analysed by the flotation method using sodium chloride saturated solution. The overall prevalence of gastrointestinal (GI) parasites was 56.6%. GI parasites were significantly (p=0.005) more common in cats with digestive symptoms (69.8%) than in clinically healthy ones (23.0%). Toxocara cati (40.2%) was the most frequently identified GI parasite, followed by Cystoisospora spp. (10.2%), Ancylostoma tubaeforme (3.7%), Taenia spp. (2.2), Giardia duodenalis (2.2%), and Toxoplasma gondii-like oocysts (0.7%). T. cati (53.3%, p<0.0001) and Cystoisospora spp. (15.6%, p<0.001) were significantly more frequently diagnosed in cats with digestive symptoms. Lack of deworming in the last three months (OR: 15.9), outdoor access (OR: 13.8), the presence of digestive symptoms (OR: 5.4), and young age (OR: 4.2) were identified as risk factors for T. cati infection by logistic regression analysis. Regardless of the age, symptoms like vomiting, diarrhoea, and inappetence were positively associated with T. cati.

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