scholarly journals First investigations into the prevalence of Cryptosporidium and Giardia spp. in Hungarian drinking water

2007 ◽  
Vol 5 (4) ◽  
pp. 573-584 ◽  
Author(s):  
J. Plutzer ◽  
M. H. Takó ◽  
K. Márialigeti ◽  
A. Törökné ◽  
P. Karanis
Keyword(s):  
Drinking Water ◽  
Water Samples ◽  
Raw Water ◽  
Forest Environment ◽  
Water Supplies ◽  
Purification Method ◽  
Cryptosporidium Oocysts ◽  
River Danube ◽  
Drinking Water Samples ◽  
Giardia Cysts

Safe drinking water is a top priority in preventing disease outbreaks and is of general concern to everyone. This study examines the occurrence of Cryptosporidium and Giardia in Hungarian drinking water supplies for the first time. A total of 76 raw and drinking water samples were examined using the U.S. EPA Method 1623. From these 15 of 34 (48.4%) raw water samples tested positive for Giardia and 7 (26.6%) for Cryptosporidium. Twelve of 45 (26.7%) drinking water samples were positive for Giardia and 6 (13.3%) for Cryptosporidium. Overall, Giardia cysts and/or Cryptosporidium oocysts were detected in 48% of the raw water samples and 35% of the drinking water samples. The highest levels in drinking water were found to be 3 oocysts/100 litres of Cryptosporidium and 63.6 cysts/100 litres for Giardia, enough to cause giardiasis. The highest levels in raw water were 1,030 cysts/100 litres for Giardia and 50 oocysts/100 litres for Cryptosporidium and higher oocyst densities were associated with source water receiving effluents from sewage treatment plants or originating from a forest environment. In addition to this monitoring, riverbank filtrated water and raw water from the River Danube in Budapest were monitored in order to ascertain protozoan removal efficiency of riverbank filtration (RBF). A total of 157 samples, including 87 samples from the River Danube and 70 samples post RBF, were examined. Cryptosporidium and Giardia were detected regularly in the river water but never in riverbank filtered water suggesting the effectiveness of RBF as a purification method. The occurrence of Cryptosporidium oocysts and Giardia cysts in the investigated water supplies may require the water utilities and water authorities in Hungary to apply additional monitoring and treatment and/or watershed controls.

scholarly journals Detection of Giardia and Cryptosporidium cysts/oocysts in watersheds and drinking water sources in Brazil urban areas

2009 ◽  
Vol 8 (2) ◽  
pp. 399-404 ◽  
Author(s):  
Maria Tereza Pepe Razzolini ◽  
Thaís Filomena da Silva Santos ◽  
Veridiana Karmann Bastos
Keyword(s):  
Drinking Water ◽  
Water Samples ◽  
Urban Areas ◽  
Water Sources ◽  
Raw Water ◽  
Protozoan Parasites ◽  
The Us ◽  
Cryptosporidium Oocysts ◽  
Us Epa ◽  
Giardia Cysts

The protozoan parasites Giardia and Cryptosporidium have been described as important waterborne disease pathogens, and are associated with severe gastrointestinal illnesses. The objective of this paper was to investigate the presence of Giardia cysts and Cryptosporidium oocysts in samples from watershed catchments and treated water sources. A total of 25 water samples were collected and examined according to the US EPA—Method 1623, 2005, consisting of 12 from drinking water and 13 from raw water. Positive samples from raw water for Giardia cysts and Cryptosporidium oocysts were 46.1 and 7.6%, respectively. In finished water, positive samples were 41.7% for Giardia cysts and 25.0% for Cryptosporidium oocysts. Concentrations of Giardia cysts found in raw water samples ranged from “not detected” to 3.4 cysts/L, whereas concentrations of Cryptoporidium oocysts ranged from “not detected” to 0.1 oocysts/L. In finished water, Giardia concentrations ranged from “not detected” to 0.06 cysts/L, and Cryptosporidium, from “not detected” to 0.01 oocysts/L. Concentrations of Giardia cysts and Cryptosporidium oocysts were not high in the samples analyzed. Nevertheless, the results of this study highlight the need to monitor these organisms in both raw and drinking water.

Distribution and removal of Giardia and Cryptosporidium in water supplies in Germany

1998 ◽  
Vol 37 (2) ◽  
pp. 9-18 ◽  
Author(s):  
Panagiotis Karanis ◽  
Dirk Schoenen ◽  
H. M. Seitz
Keyword(s):  
Drinking Water ◽  
Water Treatment ◽  
Surface Water ◽  
Water Samples ◽  
Wash Water ◽  
Raw Water ◽  
Water Supplies ◽  
Surface Water Treatment ◽  
Water Treatment Plants ◽  
Drinking Water Samples

This study has been conducted, to estimate the distribution of Giardia and Cryptosporidium in German water supplies and the removal efficiency of surface water treatment plants for Giardia and Cryptosporidium by conventional treatment. Water samples from six surface water treatment plants in different parts of Germany were simoultaneously examined for Giardia and Cryptosporidium. Investigations for both parasites were carried out in the period from July 1993 until December 1995. The results confirmed the occurrence of Giardia and Cryptosporidium in surface and raw water, in intermediate steps after treatment, in back wash water, in the first filtrate and in final water. Giardia or Cryptosporidium or both have been found in 76.2% of the investigated raw water sources. The average number of the detected Giardia cysts was 88.2/100 1 (max. 1314/100 1), and the average number of Cryptosporidium oocysts was 116/100 1 (max. 1081/100 1). In the intermediate steps (including flocculation and several steps of filtration), Giardia or Cryptosporidium or both have been found in 33.3% (50/150) of the samples. 14.9% of drinking water samples (7/47) were positive for Giardia (max. 16.8/100 1) and 29.8% (14/47) were positive for Cryptosporidium (max. 20.8/100 1). Overall, Giardia and Cryptosporidium, or both were detected in 38.3% of the drinking water samples. The parasites have been found in nearly all of the investigated backwash water samples. The filtrate of a rapid sand filter was analysed immediately after filter backwashing during the ripening period of the filter. Good elimination results were obtained by optimizing relevant water treatment process, but a low flocculant dose following sudden variation in the raw water quality, causes a breakthrough of Cryptosporidium into the treated water. Although water treatment technologies are effective to remove Giardia and Cryptosporidium, the results clearly show that Giardia and Cryptosporidium evade the filter barries in the absence of visible treatment deficiencies and low turbitidy level, and contaminate final water.

New methods for the detection of viruses: call for review of drinking water quality guidelines

2001 ◽  
Vol 43 (12) ◽  
pp. 1-8 ◽  
Author(s):  
W.O.K. Grabow ◽  
M.B. Taylor ◽  
J.C. de Villiers
Keyword(s):  
Water Quality ◽  
Cell Culture ◽  
Drinking Water ◽  
Water Samples ◽  
Raw Water ◽  
Water Supplies ◽  
Water Quality Guidelines ◽  
Quality Guidelines ◽  
Treated Drinking Water ◽  
Drinking Water Samples

Drinking water supplies which meet international recommendations for source, treatment and disinfection were analysed. Viruses recovered from 100 L-1,000 L volumes by in-line glass wool filters were inoculated in parallel into four cell culture systems. Cell culture inoculation was used to isolate cytopathogenic viruses, amplify the nucleic acid of non-cytopathogenic viruses and confirm viability of viruses. Over a period of two years, viruses were detected in 23% of 413 drinking water samples and 73% of 224 raw water samples. Cytopathogenic viruses were detected in 6% raw water samples but not in any treated drinking water supplies. Enteroviruses were detected in 17% drinking water samples, adenoviruses in 4% and hepatitis A virus in 3%. In addition to these viruses, astro- and rotaviruses were detected in raw water. All drinking water supplies had heterotrophic plate counts of <100/mL, total and faecal coliform counts of 0/100 mL and negative results in qualitative presence-absence tests for somatic and F-RNA coliphages (500 mL samples). These results call for a revision of water quality guidelines based on indicator organisms and vague reference to the absence of viruses.

Occurrence of Cryptosporidium oocysts and Giardia cysts in a conventional water purification plant

2001 ◽  
Vol 43 (12) ◽  
pp. 89-92 ◽  
Author(s):  
A. Hashimoto ◽  
T. Hirata ◽  
S. Kunikane
Keyword(s):  
Water Purification ◽  
Water Samples ◽  
Geometric Mean ◽  
Giardia Cyst ◽  
Raw Water ◽  
Observation Level ◽  
Cryptosporidium Oocysts ◽  
Purification Plant ◽  
Mean Concentration ◽  
Giardia Cysts

A one-year monitoring of Cryptosporidium oocysts and Giardia cysts was conducted at a water purification plant. A total of thirteen 50 L samples of river source water and twenty-six 2,000 L samples of filtered water (treated by coagulation-flocculation, sedimentation and rapid filtration) were concentrated using a hollow fibre ultrafiltration membrane module at a purification plant. Cryptosporidium oocysts were detected in all raw water samples with a geometric mean concentration of 400 oocysts/m3 (range 160-1,500 oocysts/m3). Giardia cysts were detected in 12/13 raw waters (92%) with a geometric mean concentration of 170 cysts/m3 (range 40-580 oocysts/m3). Probability distributions of both Cryptosporidium oocyst and Giardia cyst concentration in raw water were nearly log-normal. In filtered water samples, Cryptosporidium oocysts were detected in 9/26 samples (35%) with a geometric mean concentration of 1.2 oocysts/m3 (range 0.5-8 oocysts/m3) and Giardia cysts in three samples (12%) with 0.8 cysts/m3 (range 0.5-2 oocysts/m3). The estimated removal of Cryptosporidium oocysts and Giardia cysts was, respectively, 2.54 log10 and 2.53 log10 on the basis of geometric means, 3.20 and 3.57 log10 on the basis of 50% observation level and 2.70 and 2.90 log10 on the basis of 90% observation level.

Optimization of the Envirochek capsule method and immunomagnetic separation procedure for the detection of low levels of Cryptosporidium in large drinking water samples

2000 ◽  
Vol 41 (7) ◽  
pp. 111-117 ◽  
Author(s):  
A. Pezzana ◽  
Ph. Vilaginès ◽  
F. Bordet ◽  
D. Coquard ◽  
B. Sarrette ◽  
...  
Keyword(s):  
Drinking Water ◽  
Water Samples ◽  
Magnetic Beads ◽  
Tap Water ◽  
Immunomagnetic Separation ◽  
Sample Volume ◽  
Separation Procedure ◽  
Cryptosporidium Oocysts ◽  
Low Levels ◽  
Drinking Water Samples

The method for concentration of Cryptosporidium oocysts in large drinking water samples using the Envirocheck capsule has been optimized for the detection of low levels of oocysts. Elution from the filter by contact time and vortex agitation gave 68% oocyst recovery. Centrifugation (1,250 g; 30 min; 4°C) improved recovery to 94% without morphological damage of the oocysts. Increasing the ratio of magnetic beads to sample volume in the IMS procedure led to 69% efficiency. In these conditions, the overall recovery of the procedure was 49% as assessed with low oocysts spike doses in 100 litres tap water samples. The methodology described allows the detection of 0.1 oocyst per litre when 100 litres samples are processed.

scholarly journals Assessing protozoan risks for surface drinking water supplies in Nova Scotia, Canada

2015 ◽  
Vol 14 (1) ◽  
pp. 155-166 ◽  
Author(s):  
Wendy Krkosek ◽  
Victoria Reed ◽  
Graham A. Gagnon
Keyword(s):  
Drinking Water ◽  
Nova Scotia ◽  
Surface Water ◽  
Quality Analysis ◽  
Water Supplies ◽  
Log Reduction ◽  
Disability Adjusted Life ◽  
Life Years ◽  
Cryptosporidium Oocysts ◽  
Giardia Cysts

Protozoa, such as Cryptosporidium parvum and Giardia lamblia, pose a human health risk when present in drinking water. To minimize health risks, the Nova Scotia Treatment Standards for surface water and groundwater under the direct influence of surface water require a 3-log reduction for Giardia cysts and Cryptosporidium oocysts. This study determined the protozoan risk of municipal surface source waters in Nova Scotia, through the use of a pre-screening risk analysis of water supplies, followed by subsequent water quality analysis of the seven highest risk supplies. The water supplies were monitored monthly for 1 year to obtain baseline data that could be used for a quantitative microbial risk assessment (QMRA). The QMRA model outcomes were compared to the Health Canada health target of 10−6 disability-adjusted life years/person/year. QMRA modeling shows that the treatment facilities meet the required log reductions and disability-adjusted life year target standards under current conditions. Furthermore, based on the results of this work, Nova Scotia should maintain the current 3-log reduction standard for Giardia cysts and Cryptosporidium oocysts. The results of this study show that a pre-screening step can help to inform water sources that are particularly vulnerable to protozoan contamination, which can lead to more focused, cost-effective sampling, and monitoring programs.

scholarly journals Variability of naturally occurring fluoride in diverse community drinking-water sources, Tanna Island, Vanuatu

2021 ◽  
Author(s):  
Elizabeth Webb ◽  
Carol Stewart ◽  
Erie Sami ◽  
Samuel Kelsey ◽  
Peggy Fairbairn Dunlop ◽  
...  
Keyword(s):  
Drinking Water ◽  
Water Samples ◽  
Natural Waters ◽  
Fluoride Concentration ◽  
Sampling Period ◽  
Health Study ◽  
Volcanic Emissions ◽  
Water Supplies ◽  
Adverse Health ◽  
Drinking Water Samples

Abstract Large variations in fluoride concentrations exist in natural waters, many of which are the source of community drinking-water supplies. Determining fluoride concentrations in community drinking waters can be challenging in developing Pacific countries such as Vanuatu that have limited laboratory capacity. Knowledge of naturally elevated fluoride concentrations that cause irreversible, adverse health outcomes may allow communities the opportunity to treat and manage their drinking-water supplies. Community drinking-water samples (n = 69), sourced from groundwaters, roof catchment rainwaters, surface waters and springs, were sampled on Tanna Island, Vanuatu between 2017 and 2020. In an 18 km2 area of Western Tanna, a set of 30 groundwater-based drinking-water samples had a median fluoride concentration of 3.3 mg/L, with 20 samples >1.5 mg/L and seven samples >4.0 mg/L. These concentrations increase the risk of dental and skeletal fluorosis, respectively. Repeat resampling at five sites showed little variation over the sampling period. Rainwater-fed drinking-water supplies were lower overall and highly variable in fluoride concentrations (<0.05–4.0 mg/L, median of 0.53 mg/L), with variable inputs from volcanic emissions from Yasur volcano. We recommend a comprehensive oral health and bone health study for the whole island to determine adverse health effects of excess fluoride in this vulnerable population.

scholarly journals Presence of Giardia cysts and Cryptosporidium oocysts in drinking water supplies in northern Spain

2007 ◽  
Vol 102 (3) ◽  
pp. 882-882
Author(s):  
D. Carmena ◽  
X. Aguinagalde ◽  
C. Zigorraga ◽  
J. Crespo ◽  
J. Ocio
Keyword(s):  
Drinking Water ◽  
Northern Spain ◽  
Water Supplies ◽  
Cryptosporidium Oocysts ◽  
Giardia Cysts

scholarly journals Identification of microbial contaminants in drinking water samples of Ahmedabad using PCR

2018 ◽  
Vol 10 (3) ◽  
pp. 905-909 ◽  
Author(s):  
Rohi T Bhatt ◽  
Manisha A Nagwadiya ◽  
Sheji Chandran ◽  
Bhupesh N Yagnik
Keyword(s):  
Drinking Water ◽  
Water Samples ◽  
Bacterial Species ◽  
Fungal Species ◽  
Its Rdna ◽  
Water Supplies ◽  
Municipal Water ◽  
16S Rdna Pcr ◽  
Drinking Water Samples

Quality of drinking water is one of the greatest anxiety of water consumers with contamination of different pathogenic microorganisms especially fungi and bacteria. Water-borne pathogen contamination in water resources and related diseases are a major water quality concern throughout the world. A motivation for this study was the numerous reports about these contaminations of drinking water and the associated diseases. Water samples were collected from various locations of Ahmedabad in February 2017. The samples were analyzed by 16S rDNA PCR and ITS rDNA PCR since the DNA sequence polymorphisms in the 16S ribosomal DNA and ITS rDNA can be used for the identification of bacterial and fungal species respectively. The PCR amplicons were obtained for each water samples, were sequenced and the species identity was determined using NCBI-BLAST. Bacterial species of Aeromonas, Citrobacter and E. coli along with two fungal species were obtained from municipal water supplies and parab waters. Species of Aspergillus and Penicillium represented the most common fungi in municipal water supplies. This is the first report of molecular study from drinking water facilities of Ahmedabad. This study has clearly revealed that currently, the microbiological quality of the water is inferior for drinking purpose due to bacterial and fungal contamination. This study also showed the potential PCR based identification as a rapid technique for identifying microorganisms in drinking water samples.

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