scholarly journals Detection of Helicobacter pylori and fecal indicator bacteria in five North American rivers

2005 ◽  
Vol 3 (4) ◽  
pp. 405-422 ◽  
Author(s):  
Mary A. Voytek ◽  
Jon B. Ashen ◽  
Julie D. Kirshtein ◽  
Edward R. Landa ◽  
Lisa R. Fogarty
Keyword(s):  
North American ◽  
Fecal Indicator Bacteria ◽  
Molecular Techniques ◽  
Indicator Bacteria ◽  
Water Quality Standards ◽  
Fecal Indicator ◽  
Public Health Agencies ◽  
Health Agencies ◽  
Standard Culture ◽  
H Pylori

This study examines the use of fecal indicator bacteria (FIB) as a predictor of the presence of Helicobacter spp. A combination of standard culture and molecular techniques were used to detect and quantify FIB, Helicobacter spp. and H. pylori from five North American rivers of different size and with different land use characteristics. Primers designed to amplify genes specific to Helicobacter spp. and H. pylori were evaluated for their efficacy in detection and quantification in environmental samples. Helicobacter spp. were detected in 18/33 (55%) of river samples. H. pylori was detected in 11/33 (33%) of river samples. FIB were found in 32/33 (96%) of river samples. When FIB abundance exceeded USEPA water quality standards for single samples, Helicobacter or H. pylori were detected in 7/15 (47%) cases. No numerical correlation was found between the presence of FIB and either Helicobacter spp. or H. pylori. This suggests that the presence of FIB will be of limited use for detection of Helicobacter spp. or H. pylori by public health agencies.

An Assessment of Fecal Indicator Bacteria-Based Water Quality Standards

2008 ◽  
Vol 42 (13) ◽  
pp. 4676-4682 ◽  
Author(s):  
Andrew D. Gronewold ◽  
Mark E. Borsuk ◽  
Robert L. Wolpert ◽  
Kenneth H. Reckhow
Keyword(s):  
Water Quality ◽  
Fecal Indicator Bacteria ◽  
Quality Standards ◽  
Indicator Bacteria ◽  
Water Quality Standards ◽  
Fecal Indicator

Marker genes of fecal indicator bacteria and potential pathogens in animal feces in subtropical catchments

2019 ◽  
Vol 656 ◽  
pp. 1427-1435 ◽  
Author(s):  
Warish Ahmed ◽  
Christian O'Dea ◽  
Nicole Masters ◽  
Anna Kuballa ◽  
Oswald Marinoni ◽  
...  
Keyword(s):  
Fecal Indicator Bacteria ◽  
Indicator Bacteria ◽  
Marker Genes ◽  
Fecal Indicator ◽  
Animal Feces

scholarly journals Decay of Fecal Indicator Bacterial Populations and Bovine-Associated Source-Tracking Markers in Freshly Deposited Cow Pats

2013 ◽  
Vol 80 (1) ◽  
pp. 110-118 ◽  
Author(s):  
Adelumola Oladeinde ◽  
Thomas Bohrmann ◽  
Kelvin Wong ◽  
S. T. Purucker ◽  
Ken Bradshaw ◽  
...  
Keyword(s):  
Decay Rate ◽  
Fecal Indicator Bacteria ◽  
Decay Rates ◽  
Indicator Bacteria ◽  
Source Tracking ◽  
Fecal Indicator ◽  
Bacterial Populations ◽  
Content Type ◽  
E Coli ◽  
Decay Dynamic

ABSTRACTUnderstanding the survival of fecal indicator bacteria (FIB) and microbial source-tracking (MST) markers is critical to developing pathogen fate and transport models. Although pathogen survival in water microcosms and manure-amended soils is well documented, little is known about their survival in intact cow pats deposited on pastures. We conducted a study to determine decay rates of fecal indicator bacteria (Escherichia coliand enterococci) and bovine-associated MST markers (CowM3, Rum-2-bac, and GenBac) in 18 freshly deposited cattle feces from three farms in northern Georgia. Samples were randomly assigned to shaded or unshaded treatment in order to determine the effects of sunlight, moisture, and temperature on decay rates. A general linear model (GLM) framework was used to determine decay rates. Shading significantly decreased the decay rate of theE. colipopulation (P< 0.0001), with a rate of −0.176 day−1for the shaded treatment and −0.297 day−1for the unshaded treatment. Shading had no significant effect on decay rates of enterococci, CowM3, Rum-2-bac, and GenBac (P> 0.05). In addition,E. colipopulations showed a significant growth rate (0.881 day−1) in the unshaded samples during the first 5 days after deposition. UV-B was the most important parameter explaining the decay rate ofE. colipopulations. A comparison of the decay behaviors among all markers indicated that enterococcus concentrations exhibit a better correlation with the MST markers thanE. coliconcentrations. Our results indicate that bovine-associated MST markers can survive in cow pats for at least 1 month after excretion, and although their decay dynamic differs from the decay dynamic ofE. colipopulations, they seem to be reliable markers to use in combination with enterococci to monitor fecal pollution from pasture lands.

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