scholarly journals Assessment of microbiological and physico-chemical characterıstics of water samples in households of Bangalore city, Karnataka, India

2021 ◽  
Author(s):  
Jessen George ◽  
Siri Karthiyayani Nagaraja ◽  
Ajisha A.
Keyword(s):  
Drinking Water ◽  
Water Samples ◽  
Bacterial Species ◽  
Coliform Bacteria ◽  
Plate Count ◽  
Most Probable Number ◽  
Potential Health ◽  
Probable Number ◽  
Bangalore City ◽  
Physico Chemical

Abstract Access to safe drinking water is one of the basic human rights and is essential to human health. The present study investigated the concentration of pathogenic microbial flora and health risk in drinking water samples in households of Bangalore city, Karnataka, India. The samples were analysed for microbiological and physico-chemical parameters. In this study, most probable number and heterotrophic plate count were used to assess the microbial load. The results of the study show that most of the household water samples were contaminated with the presence of coliform bacteria. The dominant bacterial species are Escherichia coli, Salmonella, Shigella, Klebsiella and Enterobacter. The bacteria belonging to the family Enterobacteriaceae showed maximum occurrence in water samples. The overall results of the study showed that the consumption of such contaminated drinking water at the end-user point may cause potential health hazards to the inhabitants.

scholarly journals DETEKSI BAKTERI Escherichia coli DALAM AIR MINUM ISI ULANG YANG DISTERILISASI ULTRAVIOLET DI WILAYAH KECAMATAN JAGAKARSA

Jurnal BIOMA ◽  
2017 ◽  
Vol 11 (1) ◽  
pp. 73
Author(s):  
Rezki Rachmawati ◽  
Muzajjanah Muzajjanah ◽  
Yoswita Rustam
Keyword(s):  
Escherichia Coli ◽  
Drinking Water ◽  
Water Samples ◽  
Coliform Bacteria ◽  
Most Probable Number ◽  
Coliform Bacterium ◽  
Probable Number ◽  
E Coli ◽  
Biological Contamination ◽  
Drinking Water Samples

Refill Water Depot is currently more widely circulated and used as an alternative drinking water supply by the public. However the still unclear about the quality of the drinking water refill generated primarily of biological content. Parameters of biological contamination in drinking water caused by the Escherichia coli and coliform bacterium. This study aims to identify E. coli and coliforms in drinking water refill. Refill drinking water samples obtained from 16 drinking water refill from Jagakarsa subdsitrict. The method used is descriptive. Refill drinking water samples was taken and tested in the MPN (Most Probable Number) method and then to be tested in identification of E. coli. The results of testing the drinking water refill obtained 15 samples positive for coliform bacteria. Samples were positive for E. coli bacteria that sample B.1 and F.2.

scholarly journals Identifikasi Bakteri Escherichia coli dalam Air Minum Galon pada Kantin yang ada di Universitas Andalas Padang

2021 ◽  
Vol 10 (1) ◽  
pp. 23
Author(s):  
Muhammad Rayhan Braja Gitawama ◽  
Netti Suharti ◽  
Nora Harminarti
Keyword(s):  
Escherichia Coli ◽  
Drinking Water ◽  
Data Analysis ◽  
Water Samples ◽  
Coliform Bacteria ◽  
Most Probable Number ◽  
Probable Number ◽  
E Coli ◽  
Drug Agency ◽  
Drinking Water Samples

The National Food and Drug Agency has controlled the refill drinking water in drinking water depots and also controlled food at school canteens ranging from elementary, junior high, to high school, but the National Food and Drug Agency never do food quality controlling in canteens at universities. Objectives: To identified the contamination by coliform and E. coli bacteria in drinking water at Andalas University canteen. Methods: This research was descriptive to identify coliform bacteria on  15 drinking water at the Andalas University faculty canteens.  Samples were taken directly using a sterile bottle, while data analysis using Most Probable Number  (MPN)  tables 5-1-1 and the presence of E. coli bacteria colonies from drinking water samples. Results: 9 of 15 water samples were contaminated by coliform bacteria with the highest MPN index of 240/100 ml that was found in 2 samples. From 9 samples containing coliform bacteria, all of them were found to contain E. coli bacteria. Conclusion: Most of the samples were contaminated by coliform and E. coli bacteria. Drinking water served using a kettle was more contaminated than drinking water served using gallons.Keywords: Coliform, Escherichia coli, MPN

MONITORING AND ASSESSMENT OF DRINKING WATER FOR SAFE WATER SUPPLY IN RURAL HARYANA TERTIARY CARE HOSPITAL

2021 ◽  
pp. 32-34
Author(s):  
Muskaan Garg ◽  
Mahenaz Khan ◽  
Swati Mittal ◽  
Vanita Mittal ◽  
Ashwini Manhas ◽  
...  
Keyword(s):  
Drinking Water ◽  
Water Supply ◽  
Water Samples ◽  
Tertiary Care Hospital ◽  
Tertiary Care ◽  
Coliform Bacteria ◽  
Most Probable Number ◽  
Care Hospital ◽  
Probable Number ◽  
Safe Water

Background-The aim of our study is to determine the drinking water quality monitoring and assessment of safe water supply in tertiary care hospital. Water samples which are collected from different sites of Adesh Medical College and Hospital, Shahabad, Haryana were processed. Material and methods- Most Probable Number (MPN) test was done to detect the coliform bacteria in drinking water, samples were collected from different sites of AMCH, bacterial isolate was identied by culture, colony morphology, Gram's staining and biochemical characterization of bacteria. Results- Out of 40 samples of drinking water 7(17.5%) were positive in which coliform bacteria were present, while 33(82.5%) were negative. In which 1(14.2%) of the total water samples were contaminated with mix growth organism, 1(14.2%) harbored Klebsiella sp., while 5(87.5%) among 7 water samples were highly contaminated with E.coli species. Out of 7 positive samples, 4(57.1%) water sample were satisfactory, 1(14.2%) were unsatisfactory sample and 2 (28.5%) were suspicious samples. Conclusion- On the basis of the results obtained, the quality analysis revealed that most of water samples were satisfactory and were not contaminated with coliforms and various pathogenic bacteria. The water cooler samples showing positive results have E.coli while less of Klebsiella species. Therefore, we suggest that all the water sources of drinking water should be properly sanitized, continuously monitored and bacterial load should be estimated on daily basis.

scholarly journals DETEKSI BAKTERI Escherichia coli DALAM AIR MINUM ISI ULANG YANG DISTERILISASI ULTRAVIOLET DI WILAYAH KECAMATAN JAGAKARSA

Jurnal BIOMA ◽  
2017 ◽  
Vol 11 (1) ◽  
pp. 73
Author(s):  
Rezki Rachmawati ◽  
Muzajjanah Muzajjanah ◽  
Yoswita Rustam
Keyword(s):  
Escherichia Coli ◽  
Drinking Water ◽  
Water Samples ◽  
Coliform Bacteria ◽  
Most Probable Number ◽  
Coliform Bacterium ◽  
Probable Number ◽  
E Coli ◽  
Biological Contamination ◽  
Drinking Water Samples

Refill Water Depot is currently more widely circulated and used as an alternative drinking water supply by the public. However the still unclear about the quality of the drinking water refill generated primarily of biological content. Parameters of biological contamination in drinking water caused by the Escherichia coli and coliform bacterium. This study aims to identify E. coli and coliforms in drinking water refill. Refill drinking water samples obtained from 16 drinking water refill from Jagakarsa subdsitrict. The method used is descriptive. Refill drinking water samples was taken and tested in the MPN (Most Probable Number) method and then to be tested in identification of E. coli. The results of testing the drinking water refill obtained 15 samples positive for coliform bacteria. Samples were positive for E. coli bacteria that sample B.1 and F.2.

scholarly journals Comparative analysis on the effectiveness of various filtration methods on the potability of water

2015 ◽  
Vol 51 (1) ◽  
pp. 42-46
Author(s):  
S. Achio ◽  
F. Kutsanedzie ◽  
E. Ameko
Keyword(s):  
Water Samples ◽  
Plate Count ◽  
Most Probable Number ◽  
Microbiological Analysis ◽  
Sand Filters ◽  
Probable Number ◽  
Ceramic Filters ◽  
Physico Chemical ◽  
Microbiological Parameters

This paper comparatively assessed the effectiveness of potable water filtration methods, commonly used in the hinterlands in some Ghanaian communities. Physico-chemical and microbiological analysis were carried out on pond, dam and river water samples, using spectrophotometric, pour plate count and the most probable number (MPN) methods. For the unfiltered water samples the total dissolved solids (TDS) and colour were the only parameters with values within recommended standards. The other parameters, total suspended solids (TSS), turbidity, total coliforms and bacterial counts levels were above their standard recommended values. All the filtration methods showed reduction in the levels or better accepted values of the physico-chemical and microbiological parameters. The ceramic filters and the household sand filters showed outstanding results, with all analysed parameters being within the acceptable standards levels. These two methods could be promoted for use to treat untreated drinking water. It is envisaged that a combination of a number of these methods would produce even better results, especially when agents such as alum and activated carbon are included. Follow-up research in this regard is therefore recommended.

Analysis of Coliform and Colifecal Total Pollution Test on Various Types of Drinking Water Using the MPN (Most Probable Number) Method

2020 ◽  
Vol 2 (2) ◽  
Author(s):  
Wanda Aulya ◽  
Fadhliani Fadhliani ◽  
Vivi Mardina
Keyword(s):  
Drinking Water ◽  
Fecal Coliform ◽  
Pathogenic Bacteria ◽  
Microbiological Quality ◽  
Coliform Bacteria ◽  
Most Probable Number ◽  
Inspection Method ◽  
Sample Number ◽  
Probable Number ◽  
Fecal Coliform Bacteria

Water is the main source for life and also the most severe substance caused by pollution. The mandatory parameters for determining microbiological quality of drinking water are total non-fecal Coliform bacteria and Coliform fecal (Escherichia coli). Coliform bacteria are a group of microorganisms commonly used as indicators, where these bacteria can be a signal to determine whether a water source has been contaminated by bacteria or not, while fecal Coliform bacteria are indicator bacteria polluting pathogenic bacteria originating from human feces and warm-blooded animals (mammals) . The water inspection method in this study uses the MPN (Most Probable Number) method which consists of 3 tests, namely, the presumption test, the affirmation test, and the reinforcement test. The results showed that of 15 drinking water samples 8 samples were tested positive for Coliform bacteria with the highest total bacterial value of sample number 1, 15 (210/100 ml), while 7 other samples were negative. From 8 positive Coliform samples only 1 sample was stated to be negative fecal Coliform bacteria and 7 other samples were positive for Coliform fecal bacteria with the highest total bacterial value of sample number 1 (210/100 ml).

scholarly journals Escherichia coli O157:H7 in Drin Prevalence of Escherichia coli O157:H7 in Drink from Different Food Premises in Kota Samarahan Sarawak

2019 ◽  
Vol 2 (2) ◽  
pp. a13-19
Author(s):  
ELEXSON NILLIAN ◽  
AMIZA NUR ◽  
DIYANA NUR ◽  
AMIRAH ZAKIRAH ◽  
GRACE BEBEY
Keyword(s):  
Escherichia Coli ◽  
Drinking Water ◽  
Coliform Bacteria ◽  
Most Probable Number ◽  
Plain Water ◽  
Escherichia Coli O157 ◽  
Probable Number ◽  
E Coli ◽  
Preventive Actions ◽  
Stx1 Gene

Contamination of drinks with E. coli O157:H7 served in food premises such as restaurants can cause haemorrhagic colitis and haemolytic uremic syndrome to humans. The presence or absence of faecal pathogen was demonstrated using coliform group as indicator microorganisms. Therefore, this study was conducted to detect the presence of E. coli O157:H7 in drinking water from food restaurant premise in Kota Samarahan and Kuching to ensure safe and potable drinking water is served to the consumer. A total of thirty (n=30) drink samples including six types of each of the samples are cold plain water, iced tea, iced milo, syrup and iced milk tea. Most Probable Number (MPN) procedure was used in this study to enumerate the MPN values of coliform bacteria in each drink collected. A total of 53.33% (16/30) of the drink samples showed positive E. coli detection. Then, the PCR assay showed 6.25% (one out of 16 isolates) samples were positive and carried stx1 gene produced by E. coli O157:H7 in iced milo sample types. This study showed the drinks collected from food premises was contaminated with faecal contamination, which was not safe to drink by the consumer. Therefore, preventive actions should be taken to prevent foodborne illness outbreak in future

scholarly journals ANALISA MIKROBIOLOGI CEMARAN BAKTERI COLIFORM DAN SALMONELLA SP PADA TAHU DI KECAMATAN DELTA PAWAN

2019 ◽  
Vol 6 (1) ◽  
pp. 61
Author(s):  
Nenengsih Verawati ◽  
Nur Aida ◽  
Ridha Aufa
Keyword(s):  
Coliform Bacteria ◽  
Plate Count ◽  
Most Probable Number ◽  
Food Ingredient ◽  
Probable Number ◽  
West Kalimantan ◽  
Safety Standards ◽  
Confirmatory Tests ◽  
Food Safety Standards ◽  
Positive Results

Tofu is a high-protein soy-based food ingredient that is widely consumed in Indonesia. Tofu producers in Delta Pawan Subdistrict are dominated by small and medium-sized entrepreneurs whose hygiene and sanitation aspects in production activities are very under-taken. This study aims to determine the presence of Coliform and Salmonella Sp bacteria on tofu produced in Delta Pawan sub-district, Ketapang, West Kalimantan and compare with SNI01-3142-1998. The method used to detect the presence of Coliform bacteria in this study uses the Most Probable Number (MPN) method, which consists of estimator and confirmatory tests using Lactosa Broth (LB) media for the Brilliant Green Lactose Broth (BGLB) assay test for confirmation . Whereas to detect the amount of Salmonella Sp using the method of Total Plate Count (TPC) with selective media Salmonella Shigella Agar (SSA). The results of Coliform analysis in both industries found Coliform MPN values ​​of more than 2400 AMP / g samples, which indicated that they did not meet the standards set by SNI. While the results of the analysis of Salmonella Sp obtained positive results, so the two industries did not meet food safety standards.

scholarly journals Is Drinking Water Sources Safe for Drink? A Multicenter Assessment of Fecal Contamination of Drinking Water Sources in Tigray Region, Ethiopia

2019 ◽  
Author(s):  
Goyitom Gebremedhn ◽  
Abera Aregawi Berhe ◽  
Abraham Aregay Desta ◽  
Lemlem Legesse
Keyword(s):  
Drinking Water ◽  
Water Samples ◽  
Fecal Coliform ◽  
Fecal Contamination ◽  
Water Sources ◽  
Capital City ◽  
Most Probable Number ◽  
Probable Number ◽  
Tigray Region ◽  
Drinking Water Sources

Abstract Background Fecal contamination of drinking water sources is the main cause of diarrhea with estimated incidence of 4.6 billion episodes and 2.2 million deaths every year. Methods A total of 145 water samples of different source type were collected from different areas in Tigray region from August 2018 to January 2019. The water samples from each site were selected purposively which involved sampling of water sources with the highest number of users and functionality status during the study period. Most Probable Number (MPN) protocol was used for the bacteriological analysis of the samples. Results A total of 145 water samples were collected from six zones in Tigray region, Ethiopia from August 2018 to January 2019. The study indicated that 63(43.5%) of the water samples were detected to have fecal coliform which is E.coli. In Mekelle city, which is the capital city of Tigray region, three in five 34(60.7%) of the collected samples were confirmed to have fecal coliform. Water samples from health facilities were 9.48 times [AOR=9.48, 95%CI: (1.59, 56.18)] more likely to have fecal coliform. Water samples from wells were 10.23 times [AOR=10.23, 95%CI: (2.74, 38.26)] more likely to have fecal coliform than water samples from Tap/Pipe. Similarly, water samples from hand pumps were 22.28 times [AOR=22.28, 95%CI: (1.26, 393.7)] more likely to have fecal coliform than water samples from Tap/Pipe. Water samples reported to be not chlorinated were 3.51 times [AOR=3.51, 95%CI: (1.35, 9.13)] more likely to have fecal coliform than water samples from chlorinated sources. Conclusion In this study all water source, including the chlorinated drinking water sources, were found highly contaminated with fecal origin bacteria. This may be mainly due to constructional defects, poor sanitation inspection, poor maintenance, intermittent water supply and irregular chlorination.

Detection of Coliform, faecal Coliform And Total Bacterial Count From Drinking Water Of Varanasi (U.P.) India

2021 ◽  
Vol 27 (2) ◽  
Author(s):  
Alok Kumar Singh ◽  
Anand Prakash Singh ◽  
Sanjay Srivastava
Keyword(s):  
Drinking Water ◽  
Water Samples ◽  
Tap Water ◽  
Bacterial Count ◽  
Most Probable Number ◽  
Total Bacterial Count ◽  
Indicator Organisms ◽  
Cross Sectional ◽  
Probable Number ◽  
Hand Pump

In India source of drinking water at Varanasi city for common people are tap water, well, hand pump, Ganga river and stored tank water collected from bore well. All water samples were studied to assess their bacteriological characteristics and suitability for potable purposes. A cross-sectional epidemiological method was adopted to investigate the drinking water of six different sites of Varanasi city. The bacteriological examination of water samples included the most probable number of presumptive coliforms, faecal coliforms, and total bacterial count. The results showed that the total coliform count was detected in all the site. In all the methods coliforms presence was indicated. Maximum number of coliform observed in all the seasons, were from river and well water followed by hand pump, tap water and stored tank. The most common group of indicator organisms used in water quality monitoring are coliforms. These organisms are representative of bacteria normally present in the intestinal tract of mammals including human. Contamination of water may occur through different way like sewage disposal in the river, seepage of bathing near sites, fecal excreta of human, bird and other animals. Improving and expanding the existing water treatment and sanitation systems are more likely to provide good, safe and sustainable sources of water in the long term.

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