scholarly journals Removing Legionella pneumophila and biofilms from water supply systems using plant essential oils

2017 ◽  
Vol 7 (1) ◽  
pp. 67-73
Author(s):  
Ozgur Ceylan ◽  
Bulent Turasay
Keyword(s):  
Antibacterial Activity ◽  
Essential Oils ◽  
Legionella Pneumophila ◽  
Biofilm Formation ◽  
Thymus Vulgaris ◽  
Water Supply Systems ◽  
Antibiofilm Activity ◽  
Biofilm Inhibition ◽  
Thyme Oil ◽  
Lemon Oil

Antibacterial and antibiofilm activities of sage (Salvia officinalis), thyme (Thymus vulgaris), peppermint (Mentha x piperita) and lemon (Citrus limonum) essential oils (EOs), supplied as commercial preparations, were investigated on Legionella pneumophila. Results showed that all EOs had antibacterial activity. Lemon oil demonstrated the best activity, with minimum inhibition concentration (MIC) and minimum bactericidal concentration values detected as 0.83–1.25 and 1.25–3.75 μL/mL, respectively. Antibiofilm activities of EOs were studied in two ways: (1) inhibition of biofilm formation at subMIC concentrations; and (2) eradication of preformed biofilms at MIC and subMIC concentrations. The highest biofilm inhibition values were 63.3, 45.5, 35.6 and 23%, and the highest eradication values were 34.2, 16.7, 39.4 and 31.9% for lemon oil, peppermint oil, sage oil and thyme oil, respectively. Lemon oil was detected to have the highest antibiofilm activity, as with antibacterial activity. The minimum antibiofilm activity was found for thyme oil. For all the EOs, the inhibition of biofilm formation was higher than the eradication of preformed biofilms at the same concentrations. The results suggested that EOs of lemon, peppermint, sage and thyme had the potential for being used in the removal of L. pneumophila from potable water systems, due to their antibacterial and antibiofilm activities.

scholarly journals Influence of Essential Oils on Post-infection Botrytis Damage in Cut Roses1

2018 ◽  
Vol 36 (2) ◽  
pp. 45-57
Author(s):  
Ben A. Bergmann ◽  
John M. Dole
Keyword(s):  
Essential Oils ◽  
Botrytis Cinerea ◽  
Post Infection ◽  
Gray Mold ◽  
Rosa Hybrida ◽  
Thymus Vulgaris ◽  
Cut Flowers ◽  
Cinnamomum Zeylanicum ◽  
Cool Water ◽  
Thyme Oil

Abstract We assessed the degree to which 16 post-infection treatments controlled Botrytis (Botrytis cinerea Pers. ex. Fr.) damage in cut roses (Rosa × hybrida). Additional experiments examined whether essential oils (EO) of cinnamon (Cinnamomum zeylanicum Blume) leaf (CLO), clove (Eugenia caryophyllata Thunb.) bud (CBO), and thyme (Thymus vulgaris L.) (TO) could reduce damage in Botrytis-infected cut roses. The 16 treatments applied to ‘Light Orlando' cut roses differed in reducing Botrytis damage and causing phytotoxicity damage. Only the synthetic fungicide fludioxonil [applied as 0.23 g · L−1 (0.00024 oz · fl oz−1) Medallion®] resulted in the desirable combination of greatly reduced stem termination frequency due to Botrytis damage and relatively minor flower phytotoxicity. When applied to cut rose ‘Freedom' or cultivars with light colored flowers (‘Cool Water', ‘Jessika', ‘Polar Star', ‘Tiffany'), all EO aqueous solutions caused pronounced phytotoxicity damage, but only TO reduced Botrytis damage significantly compared to untreated flowers. Roses exposed to EO vapor rather than an aqueous solution tended to exhibit less phytotoxicity. Vapors of CLO and CBO tended to reduce Botrytis damage less and caused greater flower phytotoxicity than TO vapor and aqueous fludioxonil. Thyme oil vapor exposures of 4.6 and 9.1 ppm warrant further investigation. Index words: Botrytis blight, Botrytis cinerea Pers. ex. Fr., cut flowers, floriculture, fungicide, gray mold, Rosa × hybrida. Chemicals used in this study: Bacillus subtilis (Cease®), bleach (Clorox®), chlorothalonil (Daconil®), copper sulphate (Phyton® 27), fenhexamide (Elevate®), fludioxonil (Medallion®), hydrogen peroxide (ZeroTol® 2.0), iprodione (Chipco® 26019 Flo), potassium bicarbonate (Milstop®), pyraclostrobin + boscalid (Pageant® Intrinsic®). Species used in this study: Rose (Rosa × hybrida) ‘Cool Water', ‘Freedom', ‘Jessika', ‘Polar Star', ‘Tiffany', Botrytis (Botrytis cinerea Pers. ex. Fr.).

scholarly journals Anti-bacterial activity of three essential oils - An in vitro study

2019 ◽  
Vol 10 (2) ◽  
pp. 1049-1053 ◽  
Author(s):  
Geetha RV ◽  
John Rozar Raj B ◽  
Lakshmi Thangavelu
Keyword(s):  
Antibacterial Activity ◽  
Essential Oils ◽  
Streptococcus Mutans ◽  
Bacterial Species ◽  
In Vitro Study ◽  
Oral Bacteria ◽  
Zone Of Inhibition ◽  
Thyme Oil ◽  
Rosemary Oil

To conduct a study regarding the antibacterial activity of essential oils against bacteria causing Caries. Essential oils are distillates of the volatile compounds of a plant’s secondary metabolism and may act as photoprotective agents. Their curative effect has been known since antiquity. It is based on a variety of pharmacological properties which are specific for each plant species. The mouth contains a variety of oral bacteria, but only a few species of bacteria are believed to cause dental caries. Antibacterial activity of the three essential oils, Rosemary oil, Holy basil oil, Thyme oil was screened against Streptococcus mutans, using disc diffusion technique. The rosemary oil was more effective against Streptococcus mutans with a zone of inhibition of 52 mm diameter (at concentration 200 µl), Rosemary oil showed a zone of inhibition of 44 mm diameter and with thyme oil, the zone diameter was 30 mm. The results of this study showed that the essential oils at different concentrations exhibited antibacterial activity against the bacterial species tested.

scholarly journals Effect of Essential Oils on Growth Inhibition, Biofilm Formation and Membrane Integrity of Escherichia coli and Staphylococcus aureus

Antibiotics ◽  
2021 ◽  
Vol 10 (12) ◽  
pp. 1474
Author(s):  
Andrés Martínez ◽  
Marcela Manrique-Moreno ◽  
Maria C. Klaiss-Luna ◽  
Elena Stashenko ◽  
German Zafra ◽  
...  
Keyword(s):  
Essential Oils ◽  
Lipid Bilayers ◽  
Biofilm Formation ◽  
Method Development ◽  
Membrane Integrity ◽  
Thymus Vulgaris ◽  
Hydrophobic Core ◽  
Synthetic Membranes ◽  
E Coli ◽  
Microdilution Method

Biofilm as a cellular conformation confers survival properties to microbial populations and favors microbial resistance. Here, we investigated the antimicrobial, antibiofilm, antimotility, antihemolytic activity, and the interaction with synthetic membranes of 15 essential oils (EOs) on E. coli ATCC 25922 and S. aureus ATCC 29213. Antimicrobial activity of EOs was determined through microdilution method; development of the biofilm was assessed using the crystal violet assay and SEM microscopy. Results indicate that Lippia origanoides thymol–carvacrol II chemotype (LTC II) and Thymus vulgaris (TV) exhibited a significant antibacterial activity, with MIC values of 0.45 and 0.75 mg/mL, respectively. The percentage of biofilm formation inhibition was greater than 70% at subinhibitory concentrations (MIC50) for LTC II EO. The results demonstrate that these two oils had significantly reduced the hemolytic effect of S. aureus by 54% and 32%, respectively, and the mobility capacity by swimming in E. coli with percentages of decrease of 55% and 47%, respectively. The results show that LTC II and TV EOs can interact with the hydrophobic core of lipid bilayers and alter the physicochemical properties of membranes. The findings suggest that LTC II and TV oils may potentially be used to aid in the treatment of S. aureus and E. coli infections.

scholarly journals Antimicrobial and antibiofilm properties of essential oils from Piper marginatum Jacq.

2021 ◽  
Vol 10 (11) ◽  
pp. e514101119967
Author(s):  
Ana Lúcia Mendes dos Santos ◽  
Filipe Augusto Matos Araújo ◽  
Érika da Silva Matisui ◽  
Luiz Antonio Mendonça Alves da Costa ◽  
Alexandre José Macêdo ◽  
...  
Keyword(s):  
Essential Oils ◽  
Biofilm Formation ◽  
Pathogenic Bacteria ◽  
Inhibitory Concentration ◽  
Folk Medicine ◽  
Gram Negative Bacteria ◽  
Biofilm Inhibition ◽  
Future Developments ◽  
Phytochemical Constituents ◽  
Amazonian Region

A low shrub growing in the Amazonian region, Piper marginatum Jacq. has been related to the treatment of a disease variety in folk medicine, however, still lacking scientific support. This study aimed to describe the composition of essential oils obtained from leaves (EOL) and branches (EOB) of P. marginatum and their antimicrobial effects on six relevant pathogenic bacteria. A combination of GC-FID and GC-MS was used to identify the phytochemical constituents. As antimicrobial assays, the oils were screened at the minimum inhibitory concentration (MIC) of 3 µg/ml for planktonic and biofilm inhibition. EOL revealed the presence of trans–nerolidol, o–cymene, spathulenol, elemicin, and α–copaene, while EOB composition was mainly of myristicin, trans-caryophyllene, trans-nerolidol, caryophyllene oxide, α–copaene, γ–muurolene and spathulenol. The strongest inhibition of planktonic growth was achieved against Pseudomonas aeruginosa (EOB) and Escherichia coli (EOB). Overall, Gram negative bacteria were more sensitive to both EOB/EOL showing less ability of growth and biofilm formation. The Gram-positive strains seemed to react to the essential oils by massive adhesion. Our results corroborate the relevance of Piperaceae and indicate the possible use of P. marginatum in future developments of antimicrobials.

scholarly journals Antibiofilm activity of Fmoc-phenylalanine against Gram-positive and Gram-negative bacterial biofilms

2020 ◽  
Author(s):  
Himanshi Singh ◽  
Avinash Y. Gahane ◽  
Virender Singh ◽  
Shreya Ghosh ◽  
Ashwani Kumar Thakur
Keyword(s):  
Biofilm Formation ◽  
Financial Burden ◽  
Attenuated Total Reflection ◽  
Staining Procedure ◽  
Antibiofilm Activity ◽  
Biofilm Inhibition ◽  
Biochemical Assays ◽  
Mechanistic Investigation ◽  
Congo Red Staining ◽  
Antibiofilm Agents

AbstractBackgroundBiofilm associated infections are the major contributor of mortality, morbidity and financial burden in patients with bacterial infection. Molecules with surfactant behaviour are known to show significant antibiofilm effect against these infections. Thus, newly discovered antibacterial Fmoc-phenylalanine (Fmoc-F) and other Fmoc-amino acids (Fmoc-AA) with surfactant properties, could have potential antibiofilm properties.ObjectivesTo evaluate and characterise the antibiofilm activity of Fmoc-F and some Fmoc-AA against various clinically relevant bacteria.MethodsBiofilm inhibition and eradication was evaluated by crystal violet staining procedure along with scanning electron microscopy (SEM). Attenuated Total Reflection - Fourier Transform Infrared Spectroscopy (ATR-FTIR), Biochemical assays and Congo red staining were employed to investigate mechanism of antibiofilm action.ResultsWe showed that Fmoc-F not only inhibits the biofilm formation in S. aureus and P. aeruginosa, but also eradicates the already formed biofilms over the surface. Further, Fmoc-F coated glass surface resists S. aureus and P. aeruginosa biofilm formation and attachment, when biofilm is grown over the surface. The mechanistic investigation suggests that Fmoc-F reduces the ECM components such as proteins carbohydrates and eDNA in the biofilm and affect its stability via direct interactions with ECM components and/ or indirectly through reducing bacterial cell population. Finally, we showed that Fmoc-F treatment in combination with other antibiotics such as vancomycin and ampicillin synergistically inhibit biofilm formation.ConclusionsOverall, the study demonstrates the potential application of Fmoc-F and other Fmoc-AA molecules individually as well as in combination as antibiofilm agents and antibiofilm coating material for treating biofilm associated infections.

scholarly journals Essential Oils of Aromatic Plants with Antibacterial, Anti-Biofilm and Anti-Quorum Sensing Activities against Pathogenic Bacteria

Antibiotics ◽  
2020 ◽  
Vol 9 (4) ◽  
pp. 147 ◽  
Author(s):  
Marlon Cáceres ◽  
William Hidalgo ◽  
Elena Stashenko ◽  
Rodrigo Torres ◽  
Claudia Ortiz
Keyword(s):  
Escherichia Coli ◽  
Antimicrobial Activity ◽  
Quorum Sensing ◽  
Essential Oils ◽  
Pathogenic Bacteria ◽  
Bacterial Resistance ◽  
Thymus Vulgaris ◽  
Dependent Manner ◽  
Concentration Dependent Manner ◽  
Biofilm Inhibition

Both the ability of bacteria to form biofilms and communicate through quorum sensing allows them to develop different survival or virulence traits that lead to increased bacterial resistance against conventional antibiotic therapy. Here, seventeen essential oils (EOs) were investigated for the antimicrobial, antibiofilm, and anti-quorum sensing activities on Escherichia. coli O157:H7, Escherichia coli O33, and Staphylococcus epidermidis ATCC 12228. All essential oils were isolated from plant material by using hydrodistillation and analyzed by GC-MS. The antimicrobial activity was performed by using the microdilution technique. Subinhibitory concentrations of each EO were assayed for biofilm inhibition in both bacterial strains. Quantification of violacein in Chromobacterium violaceum CV026 was performed for the anti-quorum sensing activity. The cytotoxicity activity of the EOs was evaluated on Vero cell line by using MTT method. Thymol-carvacrol-chemotype (I and II) oils from Lippia origanoides and Thymus vulgaris oil exhibited the higher antimicrobial activity with MIC values of 0.37–0.75 mg/mL. In addition, these EOs strongly inhibited the biofilm formation and violacein (QS) production in a concentration-dependent manner, highlighting thymol-carvacrol-chemotype (II) oil as the best candidate for further studies in antibiotic design and development against bacterial resistance.

scholarly journals In Vitro Activity of Essential Oils Against Planktonic and Biofilm Cells of Extended-Spectrum β-Lactamase (ESBL)/Carbapenamase-Producing Gram-Negative Bacteria Involved in Human Nosocomial Infections

Antibiotics ◽  
2020 ◽  
Vol 9 (5) ◽  
pp. 272 ◽  
Author(s):  
Ramona Iseppi ◽  
Alessandro Di Cerbo ◽  
Piero Aloisi ◽  
Mattia Manelli ◽  
Veronica Pellesi ◽  
...  
Keyword(s):  
Antibacterial Activity ◽  
Klebsiella Pneumoniae ◽  
Essential Oils ◽  
Disk Diffusion ◽  
Mentha Piperita ◽  
Thymus Vulgaris ◽  
Extended Spectrum ◽  
Disk Diffusion Assay ◽  
Diffusion Assay

The aim of this study was to analyze the antibacterial activity of four essential oils (EOs), Melaleuca alternifolia, Eucalyptus globulus, Mentha piperita, and Thymus vulgaris, in preventing the development and spread of extended-spectrum β-lactamase (ESBL)-producing Escherichia coli and Klebsiella pneumoniae, metallo-beta-lactamase (MBL)-producing Pseudomonas aeruginosa and carbapenemase (KPC)-producing Klebsiella pneumoniae. A total of 60 strains were obtained from the stock collection from the Microbiology Laboratory of Hesperia Hospital, Modena, Italy. Twenty ESBL-producing E. coli, 5 K. pneumoniae, 13 KPC-producing K. pneumoniae, and 20 MBL-producing P. aeruginosa were cultured and reconfirmed as ESBL and carbapenamase producers. Polymerase chain reaction was used for the detection of genes responsible for antibiotic resistance (ESBL and KPC/MBL). Antibacterial activity of the EOs was determined using the agar disk diffusion assay, and minimal inhibitory concentrations (MICs) were also evaluated. Lastly, adhesion capability and biofilm formation on polystyrene and glass surfaces were studied in 24 randomly selected strains. M. alternifolia and T. vulgaris EOs showed the best antibacterial activity against all tested strains and, as revealed by agar disk diffusion assay, M. alternifolia was the most effective, even at low concentrations. This effect was also confirmed by MICs, with values ranging from 0.5 to 16 µg/mL and from 1 to 16 µg/mL, for M. alternifolia and T. vulgaris EOs, respectively. The EOs’ antibacterial activity compared to antibiotics confirmed M. alternifolia EO as the best antibacterial agent. T. vulgaris EO also showed a good antibacterial activity with MICs lower than both reference antibiotics. Lastly, a significant anti-biofilm activity was observed for the two EOs (*P < 0.05 and **P < 0.01 for M. alternifolia and T. vulgaris EOs, respectively). A good antibacterial and anti-biofilm activity of M. alternifolia and T. vulgaris EOs against all selected strains was observed, thus demonstrating a future possible use of these EOs to treat infections caused by ESBL/carbapenemase-producing strains, even in association with antibiotics.

scholarly journals Chemical Composition, Olfactory Analysis and Antibacterial Activity of Thymus vulgaris Chemotypes Geraniol, 4-Thujanol/Terpinen-4-ol, Thymol and Linalool Cultivated in Southern France

2012 ◽  
Vol 7 (8) ◽  
pp. 1934578X1200700 ◽  
Author(s):  
Erich Schmidt ◽  
Jürgen Wanner ◽  
Martina Höferl ◽  
Leopold Jirovetz ◽  
Gerhard Buchbauer ◽  
...  
Keyword(s):  
Antibacterial Activity ◽  
Essential Oils ◽  
Thymus Vulgaris ◽  
Gram Positive ◽  
Southern France ◽  
Food Borne ◽  
Brochothrix Thermosphacta ◽  
Olfactory Analysis ◽  
The Impact ◽  
And Staphylococcus Aureus

The essential oils of four chemotypes of Thymus vulgaris L. (Lamiaceae) were analyzed for their composition and antibacterial activity to assess their different properties. GC-MS and GC-FID analyses revealed that the essentials oils can be classified into the chemotypes thymol (41.0% thymol), geraniol (26.4% geraniol), linalool (72.5% linalool) and 4-thujanol/terpinen-4-ol (42.2% cis- and 7.3% trans-sabinene hydrate, 6.5 % terpinen-4-ol). The olfactory examination confirmed the explicit differences between these chemotypes. Furthermore, antibacterial activity was investigated against several strains of two Gram-positive ( Brochothrix thermosphacta and Staphylococcus aureus) and four Gram-negative food-borne bacteria ( Escherichia coli, Salmonella abony, Pseudomonas aeruginosa and P. fragi). All essential oil samples were demonstrated to be highly effective against Gram-positive strains, whereas the impact on Gramnegative microorganisms was significantly smaller, but still considerable. The results obtained indicate that, despite their different properties, the essential oils of selected T. vulgaris chemotypes are potent antimicrobials to be employed as useful additives in food products as well as for therapeutic applications.

scholarly journals Effect of Silver Nanoparticles on Biofilm Formation and EPS Production of Multidrug-Resistant Klebsiella pneumoniae

2020 ◽  
Vol 2020 ◽  
pp. 1-9 ◽  
Author(s):  
Muhammad Hussnain Siddique ◽  
Bilal Aslam ◽  
Muhammad Imran ◽  
Asma Ashraf ◽  
Habibullah Nadeem ◽  
...  
Keyword(s):  
Silver Nanoparticles ◽  
Biofilm Formation ◽  
Extracellular Polymeric Substances ◽  
Microtiter Plate ◽  
Cellular Protein ◽  
Antibiofilm Activity ◽  
Biofilm Inhibition ◽  
Microtiter Plate Assay ◽  
Protein Leakage ◽  
Hela Cell Lines

Antibiotic resistance against present antibiotics is rising at an alarming rate with need for discovery of advanced methods to treat infections caused by resistant pathogens. Silver nanoparticles are known to exhibit satisfactory antibacterial and antibiofilm activity against different pathogens. In the present study, the AgNPs were synthesized chemically and characterized by UV-Visible spectroscopy, scanning electron microscopy, and X-ray diffraction. Antibacterial activity against MDR K. pneumoniae strains was evaluated by agar diffusion and broth microdilution assay. Cellular protein leakage was determined by the Bradford assay. The effect of AgNPs on production on extracellular polymeric substances was evaluated. Biofilm formation was assessed by tube method qualitatively and quantitatively by the microtiter plate assay. The cytotoxic potential of AgNPs on HeLa cell lines was also determined. AgNPs exhibited an MIC of 62.5 and 125 μg/ml, while their MBC is 250 and 500 μg/ml. The production of extracellular polymeric substance decreased after AgNP treatment while cellular protein leakage increased due to higher rates of cellular membrane disruption by AgNPs. The percentage biofilm inhibition was evaluated to be 64% for K. pneumoniae strain MF953600 and 86% for MF953599 at AgNP concentration of 100 μg/ml. AgNPs were evaluated to be minimally cytotoxic and safe at concentrations of 15-120 μg/ml. The data evaluated by this study provided evidence of AgNPs being safe antibacterial and antibiofilm compounds against MDR K. pneumoniae.

Sign in / Sign up

Export Citation Format

Share Document