scholarly journals INFLUENCE of SOME CLIMATE MODIFICATION TREATMENTS on GROWTH, BIOCHEMICAL and GENETIC VARIATION BASED on PROTEIN and ISOZYME MARKERS in (Stevia rebaudiana)

2018 ◽  
Vol 7 (1) ◽  
pp. 1-16
Author(s):  
Eman Rabie ◽  
Mohamed Mubarak ◽  
Mohamed Ahmed ◽  
Eman ElSarag
Keyword(s):  
Genetic Variation ◽  
Stevia Rebaudiana ◽  
Isozyme Markers

Salt Stress-Induced Genetic Variation in Calli and Shoot Cultures of Economically Important Stevia Rebaudiana

2021 ◽  
Author(s):  
Hayat Khan ◽  
Hina Qasim ◽  
Zarghoona Zahoor ◽  
Nisar Ahmad ◽  
Arshad Iqbal ◽  
...  
Keyword(s):  
Genetic Variation ◽  
Salt Stress ◽  
Leaf Explants ◽  
Stevia Rebaudiana ◽  
Steviol Glycosides ◽  
Shoot Cultures ◽  
Stem Cuttings ◽  
Market Demand ◽  
Leaf Sample ◽  
Homogeneous Population

Abstract Stevia rebaudiana is one of the potent members of family Asteraceae and is famous for the synthesis of Steviol glycosides. Recently it is one of the economically important plants with high market demand. Seed infertility and stem cuttings are the major issues in obtaining homogeneous population, therefore, the best alternative is plant cell, tissue and organ culture. Currently, salt stress is one of the major issues worldwide. The overall objective of the current study was to investigate the effect of salt stress (100 mm NaCl) on genetic variation in calli and shoot cultures of S. rebaudiana. Sterilized leaf explants were inoculated on MS media augmented with 2,4-D (2.0 mg/L) and BAP (2.0 mg/L) for callogenesis. Shoots were obtained by using BAP (2.0 mg/L) and GA3 (0.5 mg/L) after 30 days. Callus and shoots were exposed to salt stress for 30 days. These fresh calli and shoot cultures were analyzed for genetic variation including three genes. 1: Leaf sample UDP glycosyltransferase gene restricted with ScaI 2: Callus sample gene restricted with ScaI 3: Leaf sample UDP glycosyltransferase mRNA restricted with NcoI 4: Callus sample mRNA restricted with NcoI 5: Leaf sample UDP glycosyltransferase mRNA restricted with DraIII 6: Callus sample mRNA restricted with DraIII. Only the callus sample digested with DraIII showed a variation from the mother plant. Others genes did not show any variation. It is concluded that genetic variation occurs in callus which is undifferentiated mass of cells and the variation caused may be due to salt stress. This study further need sequencing to identify the genes responsible for genetic and Somaclonal variation.

scholarly journals Variability of Anthoxanthum species in Poland in relation to geographical-historical and environmental conditions: isozyme variation

2013 ◽  
Vol 30 (1) ◽  
pp. 63-93 ◽  
Author(s):  
Maria Drapikowska
Keyword(s):  
Genetic Variation ◽  
Gene Flow ◽  
Reproductive Isolation ◽  
Environmental Conditions ◽  
Habitat Type ◽  
Environmental Variation ◽  
Small Extent ◽  
Isozyme Variation ◽  
Isozyme Markers ◽  
Isozyme Variability

Abstract Variation of 9 isozyme systems was studied in Polish populations of 3 species of the genus Anthoxanthum: the native A. odoratum s. str. L. and A. alpinum Á. Löve & D. Löve, as well as the alien A. aristatum Boiss. Results of this study show that A. odoratum is characterized by a high isozyme variability of lowland populations, weakly correlated with habitat type, and partial genetic distinctness of montane populations. Moreover, 5 isozyme markers have been identified (Pgi-2, Dia-2, Mdh, Idh, Pgm) for the allopolyploid A. odoratum. Populations of A. aristatum are highly polymorphic (P = 98%). The observed isozyme differentiation of its populations (FST = 0.087) is low and gene flow between them (Nm = 5.314) is high. The genetic variation reflects environmental variation only to a small extent and is not significantly related to the phase of chorological expansion of this species. Altitudinal vicariants, A. alpinum and A. odoratum, are characterized by morphological and isozymatic distinctness, indicating their reproductive isolation. In populations of A. alpinum, polymorphism is high (P = 76.92%), differentiation among populations is moderate (FST = 0.198), and gene flow between populations along the altitudinal transect (Nm = 1.709) is relatively low

Comparative study of HPLC methods for the Analysis of Diterpene Glycosides from Stevia rebaudiana

Planta Medica ◽  
2009 ◽  
Vol 75 (09) ◽  
Author(s):  
B Hoekstra ◽  
J Traub ◽  
K Chamberlain ◽  
S Baugh ◽  
SK Venkataraman
Keyword(s):  
Comparative Study ◽  
Stevia Rebaudiana
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