scholarly journals Influence of Genetic Variation on Morphological Diversity in Accessions of Stevia Rebaudiana Bertoni

2011 ◽  
Vol 3 (3) ◽  
Author(s):  
Raji Akintunde Abdullateef ◽  
Mohamad Osman
Keyword(s):  
Genetic Variation ◽  
Stevia Rebaudiana ◽  
Morphological Diversity ◽  
Stevia Rebaudiana Bertoni

Polysaccharides from leaves of Stevia rebaudiana (Bertoni) and anti-herpesvirus properties

Planta Medica ◽  
2013 ◽  
Vol 79 (13) ◽  
Author(s):  
LF Ceole ◽  
SS Lopes ◽  
AJ Oliveira ◽  
BP Dias Filho ◽  
CV Nakamura ◽  
...  
Keyword(s):  
Stevia Rebaudiana ◽  
Stevia Rebaudiana Bertoni

Технология выращивания стевии в открытом грунте в агроклиматических условиях приморского края

2013 ◽  
pp. 8-11
Author(s):  
М. В. Ромашова ◽  
Е. Н. Барсукова
Keyword(s):  
Stevia Rebaudiana ◽  
Stevia Rebaudiana Bertoni

Изучены элементы технологического процесса выращивания стевии ( Stevia rebaudiana Bertoni) в открытом грунте в погодно-климатических условиях Приморского края; выявлен оптимальный рельеф почвы и эффективный возраст рассады стевии; рассчитана экономическая эффективность выращивания стевии в открытом грунте.

Aktivitas Antihiperkolesterolemia Ekstrak Etanol Daun Stevia Rebaudiana Bertoni Pada Tikus Putih Jantan

2020 ◽  
Vol 2 (1) ◽  
pp. 11-20
Author(s):  
Meta Kartika Untari ◽  
Ganet Elo Pramukantoro
Keyword(s):  
Total Cholesterol ◽  
Total Cholesterol Level ◽  
Density Lipoprotein ◽  
Stevia Rebaudiana ◽  
Negative Control ◽  
Stevia Rebaudiana Bertoni ◽  
Cholesterol Levels ◽  
Water Solvent ◽  
White Rats ◽  
Group I

Hypercholesterolemia is a state of increased levels of LDL (Low Density Lipoprotein) and total cholesterol in the plasma. Stevia leaves have benefits to overcome hypercholesterolemia. The aimed of this study was to obtain ethanol extracts of Stevia rebaudiana Bertoni leaves which have activity to reduce total cholesterol levels in patients with hypercholesterolemia with effective doses. The method that will be carried out to achieve this goal was to make extracts by maceration of Stevia rebaudiana Bertoni leaf powder using a water solvent for 5 days. Testing antihypercholesterolemia activity by giving treatment to 20 male white rats. Rats were divided into 5 treatment groups. Group I was negative control, II was simvastatin control, III extract was 30 mg / 200 g BW, IV extract was 60 mg / 200 g BW, V extract was 120 mg / 200 g BW. The mice were induced by propylthiouracil 12.5 mg / day and high-fat feed for 21 days, after which the rats were given the test for 14 days. Cholesterol levels were measured on days 0, 21st and 28th. The method of determining cholesterol levels uses the Easy Touch tool. On the 35th day, a total cholesterol level was examined and data analysis was performed. The results showed that the ethanol extract of stevia leaves had antihypercholesterolemia activity, extract dose of 30 mg / 200 g BW had antihypercholesterolemia activity which was equivalent to simvastatin.

scholarly journals Salinity Effects on Gene Expression, Morphological, and Physio-Biochemical Responses of Stevia rebaudiana Bertoni In Vitro

Plants ◽  
2021 ◽  
Vol 10 (4) ◽  
pp. 820
Author(s):  
Clara Azzam ◽  
Sudad Al-Taweel ◽  
Ranya Abdel-Aziz ◽  
Karim Rabea ◽  
Alaa Abou-Sreea ◽  
...  
Keyword(s):  
Salt Tolerance ◽  
Biochemical Parameters ◽  
Large Scale ◽  
Stevia Rebaudiana ◽  
Multiple Shoots ◽  
High Salt ◽  
Stress Factors ◽  
Stevia Rebaudiana Bertoni ◽  
Nacl Concentration

Stevia rebaudiana Bertoni is a little bush, which is cultivated on a large scale in many countries for medicinal purposes and used as a natural sweetener in food products. The present work aims to conduct a protocol for stevia propagation in vitro to produce and introduce Stevia rebaudiana plants as a new sweetener crop to Egyptian agriculture. To efficiently maximize its propagation, it is important to study the influence of stress factors on the growth and development of Stevia rebaudiana grown in vitro. Two stevia varieties were investigated (Sugar High A3 and Spanti) against salt stress. Leaves were used as the source of explants for callus initiation, regeneration, multiplication and rooting. Some stress-related traits, i.e., photosynthetic pigments, proline contents, and enzyme activity for peroxidase (POD), polyphenol oxidase (PPO), and malate dehydrogenase (MDH) were studied. Murashig and Skoog (MS) medium was supplemented with four NaCl concentrations: 500, 1000, 2000, and 3000 mgL−1, while a salt-free medium was used as the control. The data revealed that salinity negatively affected all studied characters: the number of surviving calli, regeneration%, shoot length, the number of multiple shoots, number of leaf plantlets−1, number of root plantlets−1, and root length. The data also revealed that Sugar High A3 is more tolerant than Spanti. The total chlorophyll content decreased gradually with increasing NaCl concentration. However, the opposite was true for proline content. Isozyme’s fractionation exhibited high levels of variability among the two varieties. Various biochemical parameters associated with salt tolerance were detected in POD. Namely, POD4, POD6, POD 9 at an Rf of 0.34, 0.57, and 0.91 in the Sugar High A3 variety under high salt concentration conditions, as well as POD 10 at an Rf of 0.98 in both varieties under high salt concentrations. In addition, the overexpression of POD 5 and POD 10 at Rf 0.52 and 0.83 was found in both varieties at high NaCl concentrations. Biochemical parameters associated with salt tolerance were detected in PPO (PPO1, PPO2 and PPO4 at an Rf of 0.38, 0.42 and 0.62 in the Sugar High A3 variety under high salt concentrations) and MDH (MDH 3 at an Rf of 0.40 in both varieties at high salt concentrations). Therefore, these could be considered as important biochemical markers associated with salt tolerance and could be applied in stevia breeding programs (marker-assisted selection). This investigation recommends stevia variety Sugar High A3 to be cultivated under salt conditions.

scholarly journals Salt Stress Mitigation via the Foliar Application of Chitosan-Functionalized Selenium and Anatase Titanium Dioxide Nanoparticles in Stevia (Stevia rebaudiana Bertoni)

Molecules ◽  
2021 ◽  
Vol 26 (13) ◽  
pp. 4090
Author(s):  
Morteza Sheikhalipour ◽  
Behrooz Esmaielpour ◽  
Gholamreza Gohari ◽  
Maryam Haghighi ◽  
Hessam Jafari ◽  
...  
Keyword(s):  
Titanium Dioxide ◽  
Salt Stress ◽  
Titanium Dioxide Nanoparticles ◽  
Chitosan Nanoparticles ◽  
Stevia Rebaudiana ◽  
High Salt ◽  
Steviol Glycosides ◽  
Rebaudioside A ◽  
Tio2 Nps ◽  
Stevia Rebaudiana Bertoni

High salt levels are one of the significant and major limiting factors on crop yield and productivity. Out of the available attempts made against high salt levels, engineered nanoparticles (NPs) have been widely employed and considered as effective strategies in this regard. Of these NPs, titanium dioxide nanoparticles (TiO2 NPs) and selenium functionalized using chitosan nanoparticles (Cs–Se NPs) were applied for a quite number of plants, but their potential roles for alleviating the adverse effects of salinity on stevia remains unclear. Stevia (Stevia rebaudiana Bertoni) is one of the reputed medicinal plants due to their diterpenoid steviol glycosides (stevioside and rebaudioside A). For this reason, the current study was designed to investigate the potential of TiO2 NPs (0, 100 and 200 mg L−1) and Cs–Se NPs (0, 10 and 20 mg L−1) to alleviate salt stress (0, 50 and 100 mM NaCl) in stevia. The findings of the study revealed that salinity decreased the growth and photosynthetic traits but resulted in substantial cell damage through increasing H2O2 and MDA content, as well as electrolyte leakage (EL). However, the application of TiO2 NPs (100 mg L−1) and Cs–Se NPs (20 mg L−1) increased the growth, photosynthetic performance and activity of antioxidant enzymes, and decreased the contents of H2O2, MDA and EL under the saline conditions. In addition to the enhanced growth and physiological performance of the plant, the essential oil content was also increased with the treatments of TiO2 (100 mg L−1) and Cs–Se NPs (20 mg L−1). In addition, the tested NPs treatments increased the concentration of stevioside (in the non-saline condition and under salinity stress) and rebaudioside A (under the salinity conditions) in stevia plants. Overall, the current findings suggest that especially 100 mg L−1 TiO2 NPs and 20 mg L−1 Cs–Se could be considered as promising agents in combating high levels of salinity in the case of stevia.

scholarly journals Comparative Assessment of the Basic Chemical Composition and Antioxidant Activity of Stevia rebaudiana Bertoni Dried Leaves, Grown in Poland, Paraguay and Brazil—Preliminary Results

2021 ◽  
Vol 11 (8) ◽  
pp. 3634
Author(s):  
Teresa Leszczyńska ◽  
Barbara Piekło ◽  
Aneta Kopeć ◽  
Benno F. Zimmermann
Keyword(s):  
Antioxidant Activity ◽  
Chemical Composition ◽  
Vitamin C ◽  
Dietary Fiber ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Stevia Rebaudiana ◽  
Steviol Glycosides ◽  
Total Polyphenols ◽  
Stevia Rebaudiana Bertoni

This study compares the content of basic nutrients (proteins, fats, digestible carbohydrates, dietary fiber and ash), steviol glycosides, selected antioxidants (vitamin C, total polyphenols) and antioxidant activity in dried leaves of Stevia rebaudiana Bertoni cultivated in Poland, Paraguay and Brazil and available in the direct sale. The basic chemical composition was determined by standard AOAC (Association of Official Analytical Chemists) methods. Content of steviol glycosides was determined by the UHPLC-UV chromatographic method. Total polyphenols content was expressed as gallic acid equivalent (GAE) and catechins equivalent (CE). Antioxidant activity was measured as ABTS●+ free radical scavenging activity. Dried leaves of S. rebaudiana grown in Poland had significantly higher contents of dietary fiber, and lower protein and ash content, compared to those derived from Paraguay and Brazil. The former had, however, considerably higher contents of total steviol glycosides, stevioside and rebaudioside D, compared to the remaining two plants. In the Paraguay-derived dried leaves, the content of rebaudioside A, C, E and rubusoside was found to be significantly lower. Dried leaves of S. rebaudiana Bertoni, cultivated in Poland, contained substantially more vitamin C and a similar content of total polyphenols, compared to those from Brazil and Paraguay. The examined material from Brazil and Paraguay plantations showed similar antioxidant activity, while that obtained from Polish cultivation was characterized by a significantly lower value of this parameter.

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