scholarly journals Scanning electron microscopy and histological studies on the skeletal muscles in post hatching Nile tilapia (oreochromis niloticus)

2015 ◽  
Vol 28 (1) ◽  
pp. 255-265
Author(s):  
Y. Magdy ◽  
M. Attia ◽  
H. Bahgat ◽  
M. EL Magd
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Oreochromis Niloticus ◽  
Nile Tilapia ◽  
Skeletal Muscles ◽  
Histological Studies ◽  
Nile Tilapia Oreochromis Niloticus ◽  
Scanning Electron

scholarly journals Insights into the role of Moringa oleifera in ameliorating acetaminophen induced hepatotoxicity in Nile tilapia, Oreochromis niloticus

2021 ◽  
Vol 52 (4) ◽  
Author(s):  
Rema L.P. ◽  
Liji C. B.
Keyword(s):  
Protective Effect ◽  
Oreochromis Niloticus ◽  
Nile Tilapia ◽  
Moringa Oleifera ◽  
Dietary Supplementation ◽  
Hepatoprotective Effect ◽  
Histological Studies ◽  
Nile Tilapia Oreochromis Niloticus ◽  
Reparative Changes

The study was aimed to investigate the hepato-protective effect of Moringa oleifera in Nile tilapia (Oreochromis niloticus) exposed to acetaminophen. Fishes exposed to sub-lethal concentration of acetaminophen for 96 hours, were fed on feed incorporated with moringa leaf, for 21 days. Histological studies of liver of fish fed with M. oleifera leaf incorporated feed , for 21 days after 96-hour acetaminophen exposure showed significant reparative changes when compared to the control. The experiment indicate that dietary supplementation of moringa leaf had hepatoprotective effect in Nile tilapia exposed to acetaminophen.

The effects of eazymetic digestion on the elastic properties of Isolated human cereberal arteries

1977 ◽  
Vol 55 (2) ◽  
pp. 161-169 ◽  
Author(s):  
Leslie C. Damude ◽  
David A. Cope ◽  
Margot R. Roach
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Cerebral Arteries ◽  
Age Group ◽  
Internal Elastic Lamina ◽  
Solution Ph ◽  
Medial Region ◽  
Histological Studies ◽  
Elastic Lamina ◽  
Scanning Electron

Measurement of volume, pressure, and length were made on eight segments of human cerebral arteries perfused with chymotrypsin (CT) (EC 3.4.21.1) solution (pH = 7.8) for no more than 19 h, and on nine arterial segments perfused with combined enzyme (CT, trypsin (EC 3.4.21.4), elastase (EC 3.4.21.11)) solutions (pH = 7.8) for no more than 4 h. Circumferential tension–strain (and absolute radius) curves were obtained through the Law of Laplace (tension = pressure × radius). Initial and final elastances (tension/strain) were calculated after 0, 0.5, 1.0, 2.0, and 4.0 h of perfusion under the combined enzyme category, and after 0, 0.5, 1.0, 2.0, 4.0, 6.0, and 19.0 h of perfusion with CT. The initial elastance showed a significant increase (0.02 < p < 0.05) after about 6 h of perfusion. Increases in the final elastance became significant only after prolonged periods of perfusion with CT. Histological studies using light and scanning electron microscopy confirmed the removal of the elastic lamina as well as portions of the medial region. Fragmentation of the internal elastic lamina did not appear to affect the distensibility of major cerebral arteries in the 50- to 80-year-old age group.

Silk Glands and Capture Nets of Hydropsyche kozhantschikovi Martynov (Trichoptera: Hydropsychidae) Larvae

2005 ◽  
Vol 40 (2) ◽  
pp. 178-185
Author(s):  
Myoung Chul Kim ◽  
Sang Chan Park ◽  
Dong Jun Chun ◽  
Suk Woo Kang ◽  
Young Rok Seo ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Body Wall ◽  
The Body ◽  
Surrounding Area ◽  
Histological Studies ◽  
Silk Glands ◽  
Caddisfly Larvae ◽  
Scanning Electron

Histological studies of the silk glands of the caddisfly larvae of Hydropsyche kozhantschikovi Martynov demonstrated that these structure fold twice into a ‘Z’ shape with the volume in the center comparatively thicker than in other sections. The glands are more slender towards the mouth and unite with the labium. A pair of muscles, an apodeum between the muscles and the body wall, and a tendon between the muscles and the glands were observed within the thorax. We assumed that silk is spewed through these structures. Examination of the material from the inside of the silk glands showed varying composition. The center was loose and heterogeneous, while the surrounding area was homogeneous. These differences were verified through immunohistochemistry. Scanning electron microscopy show that capture nets of H. kortizantschikovi larvae have randomly arranged silk strands.

Developing protoscoleces ofEchinococcus granulosuson the outer surface of the brood capsule, detected by scanning electron microscopy

1976 ◽  
Vol 50 (2) ◽  
pp. 75-77 ◽  
Author(s):  
R. C. A. Thompson
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Echinococcus Granulosus ◽  
Outer Surface ◽  
Histological Studies ◽  
Complete Development ◽  
External Development ◽  
Scanning Electron

AbstractBy means of scanning electron microscopy, stalked protrusions were observed arising from the outer surface of intact brood capsules ofEchinococcus granulosus. Histological studies showed these protrusions to be developing protoscoleces. However, complete development is not attained and the protoscoleces eventually die. It is suggested that external development is a result of overcrowding within the brood capsule.

Pathogenesis of Human Hair Defects

1974 ◽  
Vol 32 ◽  
pp. 12-13
Author(s):  
P.S. Porter ◽  
T. Aoyagi ◽  
R. Matta
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Human Hair ◽  
Standard Techniques ◽  
Scanning Electron

Using standard techniques of scanning electron microscopy (SEM), over 1000 human hair defects have been studied. In several of the defects, the pathogenesis of the abnormality has been clarified using these techniques. It is the purpose of this paper to present several distinct morphologic abnormalities of hair and to discuss their pathogenesis as elucidated through techniques of scanning electron microscopy.

Ultrastructural Analysis of the Salivary Glands of Gromphadorhina Portentosa (Schaum)

1977 ◽  
Vol 35 ◽  
pp. 638-639
Author(s):  
P.J. Dailey
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Salivary Glands ◽  
Secretory Vesicles ◽  
The Past ◽  
Transmission Electron ◽  
Means Of Transmission ◽  
Gromphadorhina Portentosa ◽  
Scanning Electron ◽  
Topographical Relief

The structure of insect salivary glands has been extensively investigated during the past decade; however, none have attempted scanning electron microscopy (SEM) in ultrastructural examinations of these secretory organs. This study correlates fine structure by means of SEM cryofractography with that of thin-sectioned epoxy embedded material observed by means of transmission electron microscopy (TEM).Salivary glands of Gromphadorhina portentosa were excised and immediately submerged in cold (4°C) paraformaldehyde-glutaraldehyde fixative1 for 2 hr, washed and post-fixed in 1 per cent 0s04 in phosphosphate buffer (4°C for 2 hr). After ethanolic dehydration half of the samples were embedded in Epon 812 for TEM and half cryofractured and subsequently critical point dried for SEM. Dried specimens were mounted on aluminum stubs and coated with approximately 150 Å of gold in a cold sputtering apparatus.Figure 1 shows a cryofractured plane through a salivary acinus revealing topographical relief of secretory vesicles.

Two- or three-way observations of the identical cell elements within the same sections by LM, TEM and/or SEM

1978 ◽  
Vol 36 (2) ◽  
pp. 82-83 ◽  
Author(s):  
Nakazo Watari ◽  
Yasuaki Hotta ◽  
Yoshio Mabuchi
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Transmission Electron Microscopy ◽  
Fine Structure ◽  
Light Microscopy ◽  
Thin Sections ◽  
Transmission Electron ◽  
Identical Cell ◽  
Electron Microscopes ◽  
Scanning Electron

It is very useful if we can observe the identical cell elements within the same sections by light microscopy (LM), transmission electron microscopy (TEM) and/or scanning electron microscopy (SEM) sequentially, because, the cell fine structure can not be indicated by LM, while the color is; on the other hand, the cell fine structure can be very easily observed by EM, although its color properties may not. However, there is one problem in that LM requires thick sections of over 1 μm, while EM needs very thin sections of under 100 nm. Recently, we have developed a new method to observe the same cell elements within the same plastic sections using both light and transmission (conventional or high-voltage) electron microscopes.In this paper, we have developed two new observation methods for the identical cell elements within the same sections, both plastic-embedded and paraffin-embedded, using light microscopy, transmission electron microscopy and/or scanning electron microscopy (Fig. 1).

Spontaneous Cataract in Nude Athymic Mice

1977 ◽  
Vol 35 ◽  
pp. 512-513
Author(s):  
Ronald H. Bradley ◽  
R. S. Berk ◽  
L. D. Hazlett
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Nude Mouse ◽  
Cellular Immunity ◽  
Phosphate Buffer ◽  
Osmium Tetroxide ◽  
Athymic Mice ◽  
Transmission Microscopy ◽  
Mouse Lens ◽  
Scanning Electron

The nude mouse is a hairless mutant (homozygous for the mutation nude, nu/nu), which is born lacking a thymus and possesses a severe defect in cellular immunity. Spontaneous unilateral cataractous lesions were noted (during ocular examination using a stereomicroscope at 40X) in 14 of a series of 60 animals (20%). This transmission and scanning microscopic study characterizes the morphology of this cataract and contrasts these data with normal nude mouse lens.All animals were sacrificed by an ether overdose. Eyes were enucleated and immersed in a mixed fixative (1% osmium tetroxide and 6% glutaraldehyde in Sorenson's phosphate buffer pH 7.4 at 0-4°C) for 3 hours, dehydrated in graded ethanols and embedded in Epon-Araldite for transmission microscopy. Specimens for scanning electron microscopy were fixed similarly, dehydrated in graded ethanols, then to graded changes of Freon 113 and ethanol to 100% Freon 113 and critically point dried in a Bomar critical point dryer using Freon 13 as the transition fluid.

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