The effects of eazymetic digestion on the elastic properties of Isolated human cereberal arteries

1977 ◽  
Vol 55 (2) ◽  
pp. 161-169 ◽  
Author(s):  
Leslie C. Damude ◽  
David A. Cope ◽  
Margot R. Roach
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Cerebral Arteries ◽  
Age Group ◽  
Internal Elastic Lamina ◽  
Solution Ph ◽  
Medial Region ◽  
Histological Studies ◽  
Elastic Lamina ◽  
Scanning Electron

Measurement of volume, pressure, and length were made on eight segments of human cerebral arteries perfused with chymotrypsin (CT) (EC 3.4.21.1) solution (pH = 7.8) for no more than 19 h, and on nine arterial segments perfused with combined enzyme (CT, trypsin (EC 3.4.21.4), elastase (EC 3.4.21.11)) solutions (pH = 7.8) for no more than 4 h. Circumferential tension–strain (and absolute radius) curves were obtained through the Law of Laplace (tension = pressure × radius). Initial and final elastances (tension/strain) were calculated after 0, 0.5, 1.0, 2.0, and 4.0 h of perfusion under the combined enzyme category, and after 0, 0.5, 1.0, 2.0, 4.0, 6.0, and 19.0 h of perfusion with CT. The initial elastance showed a significant increase (0.02 < p < 0.05) after about 6 h of perfusion. Increases in the final elastance became significant only after prolonged periods of perfusion with CT. Histological studies using light and scanning electron microscopy confirmed the removal of the elastic lamina as well as portions of the medial region. Fragmentation of the internal elastic lamina did not appear to affect the distensibility of major cerebral arteries in the 50- to 80-year-old age group.

Field Emission Scanning Electron Microscopy Of Mouse Cerebellar Synaptic Contacts

2000 ◽  
Vol 6 (S2) ◽  
pp. 844-845
Author(s):  
O.J. Castejón ◽  
R. P. Apkarian ◽  
H. V. Castejón
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Field Emission ◽  
Cerebellar Cortex ◽  
Osmium Tetroxide ◽  
Phosphate Buffer Solution ◽  
Buffer Solution ◽  
Solution Ph ◽  
Osmium Tetroxide Solution ◽  
Scanning Electron

Samples of albino mice cerebellar cortex were processed by the cryofracture method for scanning electron microscopy and examined with the field emission scanning electron microscope (FESEM). Albino mouse cerebellar cortex was excised, cut into 1-2 mm slices and inmersed in 4% glutaraldehyde in O. l M phosphate buffer solution, pH 7.4, for 24h at 4°C; and postfixed for 1 h in a similarly buffered 1% osmium tetroxide solution. Specimens were dehydrated in a graded serie of ethanol (30, 50, 70, 80, 90 2x100%) prior to wrapping individual tissue pieces in preformed absolute ethanol filled parafilm cryofracture packets. Rapid freezing of packets was performed by plunging into LN2. First, the packet was transferred from the LN2 storage vessel with LNT chilled forceps in order to avoid themial damage. Secondly, the cooled fracture blade was removed from the LN2, the packet was orientated under the blade, and immediately struck with a heavy tool.

The Electrodeposition of Nanostructured Ni-TiN Composite Films

2009 ◽  
Vol 620-622 ◽  
pp. 727-730 ◽  
Author(s):  
Chao Cai ◽  
Jun Ying Yin ◽  
Zhao Zhang ◽  
Jian Feng Yang
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Ph Value ◽  
Composite Films ◽  
Bath Temperature ◽  
Nanocomposite Films ◽  
Solution Ph ◽  
Corrosion Properties ◽  
Tin Nanoparticles ◽  
Scanning Electron

The Ni-TiN nanocomposite films have been successfully fabricated onto commercial brass copper substrates using dc electroplating technique, and the microstructure and anti-corrosion properties of the optimized Ni-TiN nanocomposite have been respectively characterized using scanning electron microscopy (SEM). The results show that the morphology of Ni-TiN composite film is sensitively dependent on the electroplating current density, the concentration of TiN nanoparticles, the solution stirring speed, the bath temperature and the solution pH value.

Silk Glands and Capture Nets of Hydropsyche kozhantschikovi Martynov (Trichoptera: Hydropsychidae) Larvae

2005 ◽  
Vol 40 (2) ◽  
pp. 178-185
Author(s):  
Myoung Chul Kim ◽  
Sang Chan Park ◽  
Dong Jun Chun ◽  
Suk Woo Kang ◽  
Young Rok Seo ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Body Wall ◽  
The Body ◽  
Surrounding Area ◽  
Histological Studies ◽  
Silk Glands ◽  
Caddisfly Larvae ◽  
Scanning Electron

Histological studies of the silk glands of the caddisfly larvae of Hydropsyche kozhantschikovi Martynov demonstrated that these structure fold twice into a ‘Z’ shape with the volume in the center comparatively thicker than in other sections. The glands are more slender towards the mouth and unite with the labium. A pair of muscles, an apodeum between the muscles and the body wall, and a tendon between the muscles and the glands were observed within the thorax. We assumed that silk is spewed through these structures. Examination of the material from the inside of the silk glands showed varying composition. The center was loose and heterogeneous, while the surrounding area was homogeneous. These differences were verified through immunohistochemistry. Scanning electron microscopy show that capture nets of H. kortizantschikovi larvae have randomly arranged silk strands.

Ultrastructural changes in feline arterial endothelium following subarachnoid hemorrhage

1978 ◽  
Vol 48 (1) ◽  
pp. 49-57 ◽  
Author(s):  
Marc R. Mayberg ◽  
O. Wayne Houser ◽  
Thoralf M. Sundt
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Subarachnoid Hemorrhage ◽  
Ultrastructural Changes ◽  
Content Type ◽  
Physiological Pressure ◽  
Arterial Endothelium ◽  
Elastic Lamina ◽  
Fine Print ◽  
Scanning Electron

✓ Scanning electron microscopy of feline basilar arterial endothelium 4 hours and 1, 3, 5, and 7 days after subarachnoid hemorrhage (SAH) showed longitudinal furrows that correlated with angiographically demonstrated vasospasm. These ridges persisted after fixation at physiological pressure, and probably reflected medial contraction with undulation of the underlying elastic lamina. No change in endothelial cell morphology or thrombogenesis was observed as long as 7 days after SAH. There is no evidence from this study to suggest that ischemia from vasospasm is a product of thromboembolism from damaged endothelial surfaces.

Developing protoscoleces ofEchinococcus granulosuson the outer surface of the brood capsule, detected by scanning electron microscopy

1976 ◽  
Vol 50 (2) ◽  
pp. 75-77 ◽  
Author(s):  
R. C. A. Thompson
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Echinococcus Granulosus ◽  
Outer Surface ◽  
Histological Studies ◽  
Complete Development ◽  
External Development ◽  
Scanning Electron

AbstractBy means of scanning electron microscopy, stalked protrusions were observed arising from the outer surface of intact brood capsules ofEchinococcus granulosus. Histological studies showed these protrusions to be developing protoscoleces. However, complete development is not attained and the protoscoleces eventually die. It is suggested that external development is a result of overcrowding within the brood capsule.

Pathogenesis of Human Hair Defects

1974 ◽  
Vol 32 ◽  
pp. 12-13
Author(s):  
P.S. Porter ◽  
T. Aoyagi ◽  
R. Matta
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Human Hair ◽  
Standard Techniques ◽  
Scanning Electron

Using standard techniques of scanning electron microscopy (SEM), over 1000 human hair defects have been studied. In several of the defects, the pathogenesis of the abnormality has been clarified using these techniques. It is the purpose of this paper to present several distinct morphologic abnormalities of hair and to discuss their pathogenesis as elucidated through techniques of scanning electron microscopy.

Ultrastructural Analysis of the Salivary Glands of Gromphadorhina Portentosa (Schaum)

1977 ◽  
Vol 35 ◽  
pp. 638-639
Author(s):  
P.J. Dailey
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Salivary Glands ◽  
Secretory Vesicles ◽  
The Past ◽  
Transmission Electron ◽  
Means Of Transmission ◽  
Gromphadorhina Portentosa ◽  
Scanning Electron ◽  
Topographical Relief

The structure of insect salivary glands has been extensively investigated during the past decade; however, none have attempted scanning electron microscopy (SEM) in ultrastructural examinations of these secretory organs. This study correlates fine structure by means of SEM cryofractography with that of thin-sectioned epoxy embedded material observed by means of transmission electron microscopy (TEM).Salivary glands of Gromphadorhina portentosa were excised and immediately submerged in cold (4°C) paraformaldehyde-glutaraldehyde fixative1 for 2 hr, washed and post-fixed in 1 per cent 0s04 in phosphosphate buffer (4°C for 2 hr). After ethanolic dehydration half of the samples were embedded in Epon 812 for TEM and half cryofractured and subsequently critical point dried for SEM. Dried specimens were mounted on aluminum stubs and coated with approximately 150 Å of gold in a cold sputtering apparatus.Figure 1 shows a cryofractured plane through a salivary acinus revealing topographical relief of secretory vesicles.

Two- or three-way observations of the identical cell elements within the same sections by LM, TEM and/or SEM

1978 ◽  
Vol 36 (2) ◽  
pp. 82-83 ◽  
Author(s):  
Nakazo Watari ◽  
Yasuaki Hotta ◽  
Yoshio Mabuchi
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Transmission Electron Microscopy ◽  
Fine Structure ◽  
Light Microscopy ◽  
Thin Sections ◽  
Transmission Electron ◽  
Identical Cell ◽  
Electron Microscopes ◽  
Scanning Electron

It is very useful if we can observe the identical cell elements within the same sections by light microscopy (LM), transmission electron microscopy (TEM) and/or scanning electron microscopy (SEM) sequentially, because, the cell fine structure can not be indicated by LM, while the color is; on the other hand, the cell fine structure can be very easily observed by EM, although its color properties may not. However, there is one problem in that LM requires thick sections of over 1 μm, while EM needs very thin sections of under 100 nm. Recently, we have developed a new method to observe the same cell elements within the same plastic sections using both light and transmission (conventional or high-voltage) electron microscopes.In this paper, we have developed two new observation methods for the identical cell elements within the same sections, both plastic-embedded and paraffin-embedded, using light microscopy, transmission electron microscopy and/or scanning electron microscopy (Fig. 1).

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