scholarly journals SPECTROPHOTOMETRIC AND SPECTROFLUORIMETRIC DETERMINATION OF CERTAIN DIURETICS IN PURE FORMS AND IN THEIR PHARMACEUTICAL FORMULATIONS

2006 ◽  
Vol 29 (1) ◽  
pp. 33-58 ◽  
Author(s):  
Michael El-Kommos ◽  
Ahmad Ahmad ◽  
Hesham Salem ◽  
Mahmoud Omar
Keyword(s):  
Pharmaceutical Formulations ◽  
Spectrofluorimetric Determination

Application of a quinone-based fluorophore for spectrofluorimetric determination of albendazole in pure form and pharmaceutical formulations

2016 ◽  
Vol 8 (25) ◽  
pp. 5136-5141 ◽  
Author(s):  
Khalid A. M. Attia ◽  
Ahmad A. Mohamad ◽  
Mohamed S. Emara
Keyword(s):  
Charge Transfer ◽  
Complex Formation ◽  
Pharmaceutical Formulations ◽  
Pure Form ◽  
Spectrofluorimetric Method ◽  
Spectrofluorimetric Determination ◽  
Ct Complex

A spectrofluorimetric method was developed for the determination of albendazole (ALB) through charge transfer (CT) complex formation with 7,7,8,8-tetracyanoquinodimethane (TCNQ).

scholarly journals SPECTROFLUORIMETRIC DETERMINATION OF EOSIN CHEMILUMINESCENCE SYSTEM FOR THE ANALYSIS OF DABIGATRAN IN PHARMACEUTICAL FORMULATIONS

2019 ◽  
pp. 104-108
Author(s):  
DHANAPAL Y ◽  
SRUDHIVINOD V ◽  
MOHAMAD WASEEM A
Keyword(s):  
Method Development ◽  
Pharmaceutical Formulations ◽  
Calibration Graph ◽  
Eosin Y ◽  
Linear Calibration ◽  
Reaction Conditions ◽  
Quenching Effect ◽  
Spectrofluorimetric Determination ◽  
Optimum Reaction

Objectives: The objectives of this study were to develop a rapid, simple, and economical spectrofluorometric method for quantification of dabigatran from marketed formulation and its principle involved based on the protonated process of a binary mixture complex formation with eosin Y. Methods: A simple and precise spectrofluorometric technique was applied for the method development. It depends on measuring the quenching effect of the drug on the native fluorescence of eosin at excitation under the optimum reaction conditions. Results: The reaction linear calibration graph constructed between the fluorescence quenching valves flouresence intensity (ΔF) and the concentration ranges of 5–50 μg/ml. Spectrofluorometric analytical performance was validated by accuracy, precision, and specificity, and the results were satisfactory. Conclusion: This method was to develop a fast, simple, and economically applied successfully for the assay, and qualification of dabigatran tablet contains drug, also with different coformulated pharmaceutical formulations.

scholarly journals Spectrofluorimetric determination of gemifloxacin mesylate and linezolid in pharmaceutical formulations: Application of quinone-based fluorophores and enhanced native fluorescence

2014 ◽  
Vol 64 (1) ◽  
pp. 15-28 ◽  
Author(s):  
Bahia Abbas Moussa ◽  
Marianne Alphonse Mahrouse ◽  
Mahmoud Ali Hassan ◽  
Michael Gamal Fawzy
Keyword(s):  
Fluorescence Intensity ◽  
Pharmaceutical Formulations ◽  
Charge Transfer Complexes ◽  
Native Fluorescence ◽  
Factors Affecting ◽  
Fluorescence Techniques ◽  
Spectrofluorimetric Determination ◽  
High Fluorescence Intensity ◽  
High Fluorescence

Abstract Quinone-based fluorophores and enhanced native fluorescence techniques were applied for a fast quantitative analysis of gemifloxacin mesylate (GEM) and linezolid (LIN) in pharmaceutical formulations. For this purpose, three sensitive, accurate and precise spectrofluorimetric methods were developed. GEM, as an n-electron donor, reacts with 7,7,8,8-tetracyanoquinodimethane (method A) and 2,5-dichloro-3,6-dihydroxy-p-benzoquinone (method B) as п-electron acceptors, forming charge transfer complexes that exhibit high fluorescence intensity at 441 and 390 nm upon excitation at 260 and 339 nm, respectively. Method C depends on measurement of enhanced native fluorescence of LIN in phosphate buffer (pH 5) at 380 nm upon excitation at 260 nm. Experimental factors affecting fluorescence intensity were optimized. Linearity was obtained over concentration ranges 50-500, 10-60 and 20-400 ng mL-1 for methods A, B and C, respectively. The developed methods were validated and successfully applied for determination of the cited drugs in tablets.

scholarly journals Highly Sensitive Spectrofluorimetric Determination of Ephedrine Hydrochloride in Pharmaceutical Preparations

2006 ◽  
Vol 89 (5) ◽  
pp. 1263-1267 ◽  
Author(s):  
Sevgi Tatar Ulu
Keyword(s):  
Pharmaceutical Preparations ◽  
Pharmaceutical Formulations ◽  
Official Method ◽  
Good Precision ◽  
Experimental Conditions ◽  
Ephedrine Hydrochloride ◽  
Highly Sensitive ◽  
Relative Standard ◽  
Spectrofluorimetric Determination

Abstract A sensitive and specific spectrofluorimetry method was developed and validated for the quantification of ephedrine (EP) in pharmaceutical preparations. The method is based on the fluorescent enhancing reaction of EP with 7-chloro-4-nitrobenzofurazan (NBD-CI; derivatization reagent), in borate buffer of pH 9 to yield a yellow, fluorescent product. Under these experimental conditions, the derivatized product of EP had excitation and emission wavelength maxima at 458 and 516 nm, respectively. The linear range of this method was 202500 ng/mL. The detection limit was 7.3 ng/mL EP. Intra- and interday precisions of the assay at 3 concentrations within this range were 0.0371.77%. The low relative standard deviation values indicate good precision, and high recovery values indicate excellent accuracy of the method. The proposed method was applied to the determination of the examined drugs in pharmaceutical formulations, and the results indicate that the method is equally as accurate, precise, and reproducible as the official method.

scholarly journals SPECTROPHOTOMETRIC DETERMINATION OF FUROSEMIDE IN PHARMACEUTICAL FORMULATIONS – A DIDACTIC APPROACH, FROM PRACTICE TO THEORY

2017 ◽  
Vol 37 (1) ◽  
pp. 30 ◽  
Author(s):  
Adalberto Alves da Silva Neto ◽  
Wagner Felippe Pacheco ◽  
Felipe Silva Semaan
Keyword(s):  
Spectrophotometric Determination ◽  
Pharmaceutical Formulations ◽  
Historical Aspects ◽  
Theory To Practice ◽  
Spectrofluorimetric Determination ◽  
Basic Concepts ◽  
Hypertensive Drug

Spectrofluorimetric determination of furosemide in pharmaceutical – a didactical approach, from theory to practice. The purpose of this paper is to present a complete possible application of simple strategies to teach basic concepts of molecular spectrofluorimetry. An ordinary anti-hypertensive drug was chosen in order to demonstrate many theoretical and practical aspects of spectrofluorimetric determinations in an accurate, precise, and inexpensive analytical lab practice. Historical aspects and fundamentals, and their contextualization for students, are also presented, being some experiments described using cheap samples.

Development of Validated Stability Indicating HPTLC Method for Assay of Ozagrel and its Pharmaceutical Formulations

2018 ◽  
pp. 36-48 ◽  
Author(s):  
Vishal N Kushare ◽  
Sachin S Kushare
Keyword(s):  
High Performance ◽  
Linear Regression Analysis ◽  
Pharmaceutical Formulations ◽  
Base Hydrolysis ◽  
Assay Method ◽  
Related Substance ◽  
Stability Indicating ◽  
Regular Production ◽  
Hptlc Method

The present paper describes stability indicating high-performance thin-layer chromatography (HPTLC) assay method for Ozagrel in bulk drugs. The method employed TLC aluminium plates precoated with silica gel 60F-254 as the stationary phase. The solvent system consisted of toluene: methanol: triethylamine (6.5: 4.0: 0.1 v/v/v). The system was found to give compact spot for Ozagrel (Rf value of 0.40 ± 0.010). Densitometric analysis of Ozagrel was carried out in the absorbance mode at 280 nm. The linear regression analysis data for the calibration plots showed good linear relationship with r2 = 0.999 with respect to peak area in the concentration range 30 - 120 ng/spot. The developed HPTLC method was validated with respect to accuracy, precision, recovery and robustness. Also to determine related substance and assay determination of Ozagrel that can be used to evaluate the quality of regular production samples. The developed method can also be conveniently used for the assay determination of Ozagrel in pharmaceutical formulations. The limits of detection and quantitation were 4.069 and 12.332 ng/spot, respectively by height. Ozagrel was subjected to acid and alkali hydrolysis, oxidation, photochemical and thermal degradation. The drug undergoes degradation under acidic, basic, oxidation and heat conditions. This indicates that the drug is susceptible to acid, base hydrolysis, oxidation and heat. Statistical analysis proves that the method is repeatable, selective and accurate for the estimation of said drug. The proposed developed HPTLC method can be applied for identification and quantitative determination of Ozagrel in bulk drug and tablet formulation.

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