scholarly journals Screening of SRLV in herds in mid-Eastern Poland

2018 ◽  
Vol 74 (8) ◽  
pp. 532-535
Author(s):  
WIKTOR BOJAR ◽  
ANDRZEJ JUNKUSZEW ◽  
MONIKA OLECH ◽  
JACEK KUŹMAK ◽  
KLAUDIUSZ SZCZEPANIAK
Keyword(s):  
Blood Serum ◽  
Detailed Analysis ◽  
Elisa Test ◽  
Serological Response ◽  
Flock Size ◽  
Serological Analysis ◽  
Serum Samples ◽  
Specific Antibodies ◽  
Merino Sheep ◽  
Detailed Diagnosis

The aim of the study was to evaluate the epidemiological condition of SRLV infections in nucleus flocks of sheep. The surveillance of Lentivirus infections was conducted in sheep flocks from the mid-Eastern part of Poland: the area of Lubelskie, Świętokrzyskie and Podkarpacie provinces. Ninety eight flocks were chosen for a detailed analysis. They included 6,470 mother ewes belonging to 15 breeds and lines: Polish Merino sheep, Polish Lowland sheep, Wielkopolska sheep, Żelaźnieńska sheep, Uhruska sheep, Olkuska sheep, Polish Heath sheep, Świniarka sheep, Polish Pogorze sheep, Podhale Zackel sheep, Black-headed sheep, Ile de France, Berrichon du Cher, BCP and SCP. The identification of the infected animals and the assessment of the epidemiological condition of the flocks was carried out using a serological analysis of blood serum samples. The assay of MVV-specific antibodies in blood serum was performed using the ELISA test. The results obtained showed that the highest rate of flocks with at least one animal with a positive serological response was in the Podkarpacie Province (71.43%), and the lowest rate (27.03%) was observed in Lubelskie Province. The analysis of the rate of the sheep infected with SRLV indicated that the greatest number of seropositive animals was highest in Podkarpackie Province (19.9%), which corresponds to the results for the flocks, and was significantly higher (p ≤ 0.01) than in Świętokrzyskie Province (11.35%) and Lubelskie Province (9.20%). The flock size turned out to be a factor which significantly influenced the prevalence of SRLV infections. The prevalence was highest (13.6%) in the flocks exceeding 100 sheep, and decreased together with the decreasing number of heads in the flock. It must be stressed that the problem of SRLV infections is very complex and depends on many factors. Therefore it is necessary to carry out a detailed diagnosis of the factors leading to expansion of the virus in sheep flocks before developing flock protection programs...

scholarly journals Serological monitoring of ornitobacteriosis in broilers in South Banat district

2014 ◽  
Vol 68 (3-4) ◽  
pp. 197-205
Author(s):  
Pavle Gavrilovic ◽  
Nikola Vaskovic ◽  
Milijan Jovanovic
Keyword(s):  
Respiratory Tract ◽  
Clinical Symptoms ◽  
Respiratory Infections ◽  
Natural Infection ◽  
Clinical Manifestations ◽  
Serological Response ◽  
Serum Samples ◽  
Exact Test ◽  
Specific Antibodies ◽  
Ornithobacterium Rhinotracheale

Ornithobacterium rhinotracheale is a relatively recently discovered bacterium and its role in the pathology of avian respiratory infections has not yet been clarified. Since there was no data relating to the prevalence of this infection in Serbia at the time of carrying out our investigations, we decided to explore the prevalence of the infection in broilers and its influence on clinical manifestations at the selected apizootiological area with developed poultry industry. A total of 430 blood samples from 26 flocks of broilers of different ages, from five municipalities were taken for examination. The serum samples were tested by ELISA for the presence of specific antibodies to the agent. Epizootiological investigation was carried out based on the results obtained with serological testing and epizootiological data, collected from the farms. The data were analyzed statistically to identify association between the infection and manifestation of clinical symptoms by Fisher?s exact test. Seropositive chickens were detected in 16 out of 26 examined broiler flocks at the age of 3 to 56 days. The percentage of seropositive samples per flock was 5-30%. The titer values of specific antibodies ranged from 946 to 6886. Serological response to O. rhinotracheale was evidenced in five flocks which had clinical symptoms in the form of respiratory tract disorders or stunting. However, specific antibodies against the agent were discovered in 11 flocks which did not show clinical symptoms. Statistical analysis revealed no association between the presence of infection and the appearance of clinical symptoms (p = 0.1213). The results are in agreement with those of other authors who investigated the prevalence of this infection and its manifestations in other countries. The present investigation determined indirectly, serologically a presence of O. rhinotracheale in the majority of examined broiler flocks (61,54%) and a small average number of individual seropositive chickens per flock - 10 % of all examined samples. Although O. rhinotracheale is wide-spread in intensive broiler production in South Banat its presence does not always lead to clinical manifestation of respiratory tract disorders. Seropositive chickens are detected in the first week of life, which indicates the presence of maternal antibodies. Presence of antibodies as the response of organism to the natural infection is detected in chickens after four weeks of age.

scholarly journals Prevalence of SRLV infections in various sheep breeds

2018 ◽  
Vol 74 (8) ◽  
pp. 517-519
Author(s):  
WIKTOR BOJAR ◽  
ANDRZEJ JUNKUSZEW ◽  
MONIKA OLECH ◽  
JACEK KUŹMAK ◽  
KLAUDIUSZ SZCZEPANIAK
Keyword(s):  
Blood Serum ◽  
Elisa Test ◽  
Infection Prevalence ◽  
Breeding Strategies ◽  
Sample Collection ◽  
Serological Reaction ◽  
Specific Antibodies ◽  
Sheep Breeds ◽  
Selection Of

The aim of the study was to determine the prevalence of SRLV infection in sheep breeds farmed in mid-Eastern Poland. Out of 6,470 mother ewes kept in 98 nucleus flocks of various sizes, 2,924 belonging to 15 breeds and lines were selected for serum sample collection. The selection of the animals analyzed was carried out using stratification which enabled the determination of infection prevalence with an error margin no greater than 8%. The assay of SRLV-specific antibodies in blood serum was performed using the ELISA test. The analyses revealed a large diversity of prevalence between the sheep breeds studied. The lowest prevalence was observed in Uhruska sheep, in which only 5.07% had a positive serological reaction, despite the fact that the number of animals of this breed included in the analysis was large (1675 mother ewes). The prevalence for this variety was over 5-7 times lower in comparison to Wielkopolska sheep (35%), Polish Pogorze sheep (22.73%), Podhale Zackel sheep (29.03%) and over 10 times lower than Świniarka sheep (57.04%). The results should be used for developing breeding strategies and establishing anti-SRLV programs..

scholarly journals Comparative investigations of sensitivity and specificity of immunoenzyme probe and inhibition hemagglutination test in serological diagnostics of newcastle disease in poultry

2009 ◽  
Vol 63 (1-2) ◽  
pp. 37-44
Author(s):  
Nenad Milic ◽  
Jakov Nisavic ◽  
Marina Radojicic ◽  
Marina Sekler ◽  
Kazimir Matovic ◽  
...  
Keyword(s):  
Newcastle Disease Virus ◽  
Blood Serum ◽  
Disease Virus ◽  
Sensitivity And Specificity ◽  
Newcastle Disease ◽  
Hemagglutination Inhibition ◽  
Serum Samples ◽  
Specific Antibodies ◽  
Hemagglutination Inhibition Test ◽  
Serological Diagnostics

Comparative investigations of the sensitivity and specificity of the indirect immunoenzyme probe - iELISA and the hemagglutination inhibition test (HI test) in serological diagnostics of the Newcastle disease in poultry were carried out using samples of blood serum taken from non-vaccinated and vaccinated poultry. A total of 14 samples of blood serum from non-vaccinated poultry were examined using the immunoenzyme probe - iELISA, and nine of these were found to be positive to the presence of specific antigen against the Newcastle disease virus, while two samples were suspect, and no presence of specific antibodies was established in three samples. Examinations of 82 samples of blood serum from vaccinated poultry for the presence of specific antibodies against the Newcastle disease virus established their presence in 80 serum samples, while one sample was suspect and one sample was negative. The values of the titer of specific antibodies in blood serum samples of vaccinated and non-vaccinated poultry established using the hemagglutination inhibition test (HI test) ranged from 1:2 to 1:32.

scholarly journals Examining the presence of specific antibodies against Salmonella enteritidis in vaccinated and unvaccinated poultry

2016 ◽  
Vol 70 (1-2) ◽  
pp. 3-12
Author(s):  
Marina Radojicic ◽  
Maja Markovic ◽  
Jakov Nisavic ◽  
Dejan Krnjaic ◽  
Nemanja Zdravkovic
Keyword(s):  
Blood Serum ◽  
Salmonella Enterica ◽  
Salmonella Enteritidis ◽  
Diagnostic Methods ◽  
Serum Samples ◽  
Specific Antibodies ◽  
Kappa Test ◽  
Agglutination Method ◽  
Serum Testing ◽  
Serological Diagnostics

Introduction. The objective of our research was to point to the significance of serological diagnostic methods, that is, competitive immunoenzyme test - cELISA as well as the method of classical agglutination of O and H salmonella antigen, for specific antibodies against Salmonella enterica subspecies enterica serovar Enteritidis presence and titre detection in blood serum samples of bothvaccinated and unvaccinated poultry. Material and methods. In our work, we have used commercial competitive immunoenzyme test - cELISA and classical agglutination method with O and H antigens of Salmonella enterica subspecies enterica serovar Enteritidis. Comparative testing included 177 blood serum samples of poultry, out of which 137 was from unvaccinated and 40 from vaccinated individuals originating from majority of poutry farms. Results. In 74 blood serum samples, that is 54,01% out of the total of tested samples originating from unvaccinated flocks, by the use of cELISA test, there were found specific antibodies against S. Enteritidis, while by the method of classical agglutination specific antibodies against O antigen 1,9 and 12 were found in 58 samples, that is in 42,34% , and specific antibodies against H antigen g and m were found in 61 samples,what was 44,53% . In all the tested blood serum samples of vaccinated poultry, there was determined the presence of of specific antibodies against S. Enteritidis, both by the use of competitive cELISA method and classical agglutination with somatic O and flagellar H antigens. By the statistical analysis of the results obtained by the method of classical agglutination and cELISA use of kappa test, there was found out a very good compliance (kappa=0,813). Conclusion. Based on the compared results of blood serum testing on the presence of specific antibodies against S. Enteritidis, it can be concluded that cELISA and classic agglutination with O and H antigen methods have a significant place in serological diagnostics of poultry samonelosis, because their application enables detection of the titre of specific antibodies against S. Enteritidis presence in the population of unvaccinated poultry sensitized with antigens of the mentioned causative agent, as well as in unvaccinated animals.

scholarly journals Association of bovine uterine involution disturbances with serum neuropeptide concentrations

2020 ◽  
Vol 13 (9) ◽  
pp. 1854-1857
Author(s):  
Aida Abultdinova ◽  
Isatay Jakupov ◽  
Joachim Roth ◽  
Klaus Failing ◽  
Axel Wehrend ◽  
...  
Keyword(s):  
Blood Serum ◽  
Dairy Cows ◽  
Serum Levels ◽  
Elisa Test ◽  
Normative Values ◽  
Uterine Disease ◽  
Serum Concentrations ◽  
Serum Samples ◽  
Uterine Involution ◽  
Significant Difference

Background and Aim: Puerperal diseases influence fertility and should be diagnosed as soon as possible. This study aimed to evaluate the applicability of serum concentrations of substance P (SP), vasoactive intestinal polypeptide (VIP), and interleukin (IL)1β in the early diagnosis of uterine involution disturbances. Materials and Methods: Blood serum samples of 86 dairy cows from six different farms were harvested within the first 20 days after calving from cows with uterine involution disturbances and healthy controls, respectively. Serum concentrations for SP, VIP, and IL-1β were determined using commercially available ELISA test kits. Statistical analyses included timely changes in blood serum levels and group comparisons of healthy cows and cows with uterine disease. Results: SP concentrations increased significantly within 20 days after calving (p<0.04) with no significant difference observed between the groups. Moreover, no significant differences were found between VIP and log IL-1β. Conclusion: Results showed that none of the examined serum parameters seems suitable as indicator of uterine involution disorders. Due to the timely changes in serum concentrations of SP after calving, a correlation to diseases might not be precluded. Further research is needed as regards the establishment of normative values concerning this parameter.

scholarly journals Establishing presence of antibodies against bovine respiratory syncytial virus (BRSV), parainfluenza virus 3 (PI3) and bovine herpesvirus 1 (BHV 1) in blood serum of cattle using indirect immunoenzyme probe

2009 ◽  
Vol 63 (3-4) ◽  
pp. 145-152 ◽  
Author(s):  
Horea Samanc ◽  
Nenad Milic ◽  
Velibor Stojic ◽  
Dejan Knezevic ◽  
Ivan Vujanac ◽  
...  
Keyword(s):  
Respiratory Syncytial Virus ◽  
Blood Serum ◽  
Bovine Herpesvirus ◽  
Bovine Respiratory Syncytial Virus ◽  
Parainfluenza Virus ◽  
Serum Samples ◽  
Bovine Herpesvirus 1 ◽  
Specific Antibodies ◽  
Herpesvirus 1 ◽  
Syncytial Virus

A total of 92 samples of bovine blood serum were examined for the presence of antibodies against the bovine respiratory syncytial virus using indirect immunoenzyme probe - iELISA. Specific antibodies against the bovine respiratory syncytial virus (BRSV) were established in 46, or 50% blood serum samples. Investigations of the 92 blood serum samples of cattle for the presence of antibodies against the parainfluenza virus 3 (PI 3), revealed their presence in 77, or 83.69% of the samples, and the presence of antibodies against the bovine herpesvirus 1 (BHV 1) was established in 19, or 20.65% of the samples.

scholarly journals Prevalence of CAEV infections in goat herds

2018 ◽  
Vol 74 (8) ◽  
pp. 536-539 ◽  
Author(s):  
TOMASZ M. GRUSZECKI ◽  
MONIKA OLECH ◽  
JACEK KUŹMAK ◽  
EMILIA BAGNICKA ◽  
ANNA SZYMANOWSKA ◽  
...  
Keyword(s):  
Blood Serum ◽  
Infection Rate ◽  
Detrimental Effect ◽  
Elisa Test ◽  
Serum Samples ◽  
Saanen Goat ◽  
Boer Goat ◽  
Prevalence Level ◽  
Goat Population ◽  
Boer Goats

The aim of the study was to analyze the occurrence of caprine arthritis encephalitis virus (CAEV) in white improved goats, colored improved goats, Saanen goats, Alpine goats and Boer goats, raised in three regions of Poland. The analysis of CAEV infection levels was carried out in goat herds from the provinces in mid-Eastern Poland. The analysis included four goat herds. In total, they encompassed 251 herds of the following breeds: white improved goat, colored improved goat, Saanen goat, Alpine goat and Boer goat. The identification of the infected animals and the assessment of the epidemiological condition of the herds was carried out using serological analysis of blood serum samples. The assay of CAEV-specific antibodies in blood serum was performed using ELISA test. As a result of the observations it was established that with regards to the provinces, the prevalence level ranged from 35.40% in Mazowieckie province to 81.10% in Lubelskie province, and the average infection rate of animals in the studied region was 51.60%. As far as the breeds are concerned, it was found that the observed infection rate of the Saanen goat population was significantly higher (p ≤ 0.05) than the rates for improved white goat (48.90%) and Alpine goat (50.00%). In relation to the colored improved goat, the calculated statistical difference was greater (p ≤ 0.01), and the percentage of infected animals was 32.10%. The analysis of the infection rate in the Boer goat herd revealed that 66.70% of animals are seropositive, and the statistical analysis did not show any significant differences in prevalence compared to other studied breeds. To sum up, it should be noted that the improvement of the situation may be attributed to consistent examination of goats and development of CAEV-elimination programs for herds. When creating programs aimed at the elimination of the virus it is also important to remember to raise the awareness of the farmers with regards to the detrimental effect of CAEV infections..

scholarly journals Incidence of Village Chicken Diseases in Eastern Shewa Zone, Ethiopia: The Case of Newcastle and Infectious Bursal Disease

2016 ◽  
Vol 1 (4) ◽  
pp. 1-14
Author(s):  
Jarso DM
Keyword(s):  
Incidence Rate ◽  
Age Groups ◽  
Simple Random Sampling ◽  
Elisa Test ◽  
Infectious Bursal Disease ◽  
Flock Size ◽  
Serum Samples ◽  
Village Chicken ◽  
Bursal Disease

A longitudinal study was carried out from September 2014 to May 2015 on village chicken of Lume district for the aim of determining incidence rate of mortality of Newcastle disease (NCD) and infectious bursal disease ( IBD) and the associated risk factors. In addition in a retrospective survey past occurrence of these diseas e was assessed. Simple random sampling method was used to select the peasant associations (PAs) and the households. Owners and veterinary field workers perception on chicken diseases was collect ed from 120 respondents through structured questionnaire. The majority (75%) of the respondents put diseases as major causes of village chicken mortality, out of which 78.3% of the respondents indicated NCD locally known as “Fangle” as the leading disease that cause mortality of chicken in the village.Of the 1358 reg istered chicken, 202 (14.9%) survived the entire follow - up period. A total of 843 chickens found dead of NCD outbreak during the follow - up period. The general mortality rate was 62.1% whereas the incidence rate was113.2 cases per 1000 chicken month.Over th e duration of the study, serum samples of 521 chickens were collected to confirm the cause of the outbreak, 242 from sick and 279 from apparently health chicken. Serology using HAI and I - ELISA test were conducted to determine the seroprevalence of NCD and IBD, respectively. In total 28.6% (149/521) and 20.7% (108/521) were positive for NCD and IBD, respectively. Among the 242 sera collected from clinically diseased chicken 61.6% (149/242) and 38.4% (93/242) were positive for NCD and IBD, respectively. Statis tically significant ( p<0.05 ) difference in prevalence of NCD was found between highland and lowland; chicken flock size and sampling months. Statistically significant ( p<0.05 ) difference in seroprevalence of IBD was found between different age groups; hous ehold flock size and sampling months. This study has shown that NCD and IBD are one of the major infectious diseases threatening the survival and productivity of traditionally managed local chickens in East Showa zone. Thu s, routine vaccination program is recommended.

scholarly journals SEROPREVALENCE OF SALMONELLA SPP. ON IMPORT BOARS FROM DENMARK TO SERBIA

2014 ◽  
Vol 6 (2) ◽  
Author(s):  
Nenad Stojanac ◽  
Ognjen Stevančević ◽  
Božidar Savić ◽  
Ivan Stančić ◽  
Aleksandar Potkonjak ◽  
...  
Keyword(s):  
Blood Serum ◽  
Cross Sectional Study ◽  
Elisa Test ◽  
Time Span ◽  
Sectional Study ◽  
Serum Samples ◽  
Salmonella Spp ◽  
Cross Sectional ◽  
One Year ◽  
Swine Herds

The sera from 120 boars from 3 farrow-to-finish swine herds in Serbia were examined for Salmonella spp. antibodies in a cross-sectional study using an ELISA test. A total of 120 blood serum samples from boars imported from Denmark were examined. All boars were seronegative to Salmonella during the import, while after one year Salmonella spp. seroprevalence ranging from 0% to 45% was found in 3 herds using two tests in the time span of one year. Only on one farm during the import in 2011, after a year boars were still seronegative. A year later on the same farm, in boars imported in 2012 the seroprevalence was 30%. Seroprevalence of Salmonella spp. in boars tested on all farms was 24,17%.

scholarly journals Association of bovine uterine involution disturbances with serum neuropeptide concentrations

2019 ◽  
Author(s):  
Aida Abultdinova ◽  
Issatay Jakupov ◽  
Joachim Roth ◽  
Klaus Failing ◽  
Axel Wehrend ◽  
...  
Keyword(s):  
Blood Serum ◽  
Dairy Cows ◽  
Serum Levels ◽  
Elisa Test ◽  
Normative Values ◽  
Uterine Disease ◽  
Serum Concentrations ◽  
Serum Samples ◽  
Uterine Involution ◽  
Significant Difference

Abstract Background: Puerperal diseases influence fertility and should be diagnosed as soon as possible. The present study aimed to evaluate the applicability of serum concentrations of substance P (SP), vasoactive intestinal polypeptide (VIP) and IL‑1β in the early diagnosis of uterine involution disturbances. Blood serum samples of 86 dairy cows from 6 different farms were harvested within the first 20 days after calving from cows with uterine involution disturbances and healthy controls, respectively. Serum concentrations for SP, VIP and IL‑1β were determined using commercially available ELISA test kits. Statistical analyses included timely changes in blood serum levels and group comparisons of healthy cows and cows with uterine disease. Results: SP-concentrations increased significantly within 20 days after calving (P < 0.04) with no significant difference between the groups. No significant differences could be shown for VIP and logIL-1β. Conclusion: None of the examined serum parameters seems suitable as indicator of uterine involution disorders. Due to the timely changes in serum concentrations of SP after calving, a correlation to diseases might not be precluded though. Further research is needed with regard to the establishment of normative values concerning this parameter.

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