scholarly journals Prevalence of CAEV infections in goat herds

2018 ◽  
Vol 74 (8) ◽  
pp. 536-539 ◽  
Author(s):  
TOMASZ M. GRUSZECKI ◽  
MONIKA OLECH ◽  
JACEK KUŹMAK ◽  
EMILIA BAGNICKA ◽  
ANNA SZYMANOWSKA ◽  
...  
Keyword(s):  
Blood Serum ◽  
Infection Rate ◽  
Detrimental Effect ◽  
Elisa Test ◽  
Serum Samples ◽  
Saanen Goat ◽  
Boer Goat ◽  
Prevalence Level ◽  
Goat Population ◽  
Boer Goats

The aim of the study was to analyze the occurrence of caprine arthritis encephalitis virus (CAEV) in white improved goats, colored improved goats, Saanen goats, Alpine goats and Boer goats, raised in three regions of Poland. The analysis of CAEV infection levels was carried out in goat herds from the provinces in mid-Eastern Poland. The analysis included four goat herds. In total, they encompassed 251 herds of the following breeds: white improved goat, colored improved goat, Saanen goat, Alpine goat and Boer goat. The identification of the infected animals and the assessment of the epidemiological condition of the herds was carried out using serological analysis of blood serum samples. The assay of CAEV-specific antibodies in blood serum was performed using ELISA test. As a result of the observations it was established that with regards to the provinces, the prevalence level ranged from 35.40% in Mazowieckie province to 81.10% in Lubelskie province, and the average infection rate of animals in the studied region was 51.60%. As far as the breeds are concerned, it was found that the observed infection rate of the Saanen goat population was significantly higher (p ≤ 0.05) than the rates for improved white goat (48.90%) and Alpine goat (50.00%). In relation to the colored improved goat, the calculated statistical difference was greater (p ≤ 0.01), and the percentage of infected animals was 32.10%. The analysis of the infection rate in the Boer goat herd revealed that 66.70% of animals are seropositive, and the statistical analysis did not show any significant differences in prevalence compared to other studied breeds. To sum up, it should be noted that the improvement of the situation may be attributed to consistent examination of goats and development of CAEV-elimination programs for herds. When creating programs aimed at the elimination of the virus it is also important to remember to raise the awareness of the farmers with regards to the detrimental effect of CAEV infections..

scholarly journals Risk for infection by Lyme disease cause in green surfaces maintenance workers in Belgrade

2007 ◽  
Vol 64 (5) ◽  
pp. 313-318 ◽  
Author(s):  
Milena Krstic ◽  
Novica Stajkovic
Keyword(s):  
Lyme Disease ◽  
Infection Rate ◽  
Risk Index ◽  
Dark Field ◽  
Elisa Test ◽  
Control Group ◽  
Serum Samples ◽  
Tick Infection ◽  
Dark Field Microscopy ◽  
Tick Infection Rate

Background/Aim. Lyme disease (LD) is a cosmopolitan disease from the group of zoonoses that in humans can affect skins, joints, heart and/or nervous system. Research conducted in endemic areas, shows not only that the population, which lives or works in tick habitats, is in a high risk for getting infected by LD cause, but also that every enviroment itself is specific in regard to both vectors and B. burgdorferi species. In our enviroment, research of seropositivity to B. burgdorferi in persons exposed to vectors of LD have not been conducted. The aim of this study was to determine whether there was a higher risk for getting B. burgdorferi infection in the fieldworks of JKP "Zelenilo Beograd" than in other persons. Methods. Risk for getting LD in the fieldworkers of JKP "Zelenilo Beograd" (park-maintanance company) was determined according to seropositivity, the number and infection rate of LD vectors, and entomological risk index (ERI) value. Serum samples of 34 fieldworkers of JKP "Zelenilo Beograd", and 35 persons of the control group, were tested for the presence of specific IgM and IgG anti-B. burgdorferi antibodies by using ELISA test. In the research localities, the number of ticks was estimated by using the flag hours method, and tick infection rate was determined by dark field microscopy, with magnification rate of 400?. The data were analyzed statistically using ?2 test, and Pearson's correlation analysis. Results. Out of 34 fieldworkes serum samples, 8 (23.5%) were positive on anti-B. burgdorferi antibody presence, while in the control group only one (2.9%) sample was positive (p < 0,05). Seropositivity rate varied from 0-66.6% among localities. Flag hours value among the localities ranged from 8.2-29.9, and tick infection rate ranged from 10.8- 22.3%. ERI value was 0.03-0.15. Correlation between flag hour rate and seropositivity, tick infection rate and seropositivity, and also ERI and seropositivity was established. Conclusion. Frequency of B. burgdorferi seropositivity was significantly higher in green-surface maintenance workers, than in the control group not exposed to these habitats. A significant correlation was found between anti-B. burgdorferi antibodies findings and the number of ticks, tick infection rate, and ERI. The results obtained in our study indicate that there was a significantly higher risk for getting LD cause in the fieldworkers than in the control group. .

scholarly journals Occurrence of Leptospirosis in Domestic Animals Reared on Exposed or Non-Exposed to Flood Areas of Eastern Poland

2012 ◽  
Vol 56 (4) ◽  
pp. 489-493 ◽  
Author(s):  
Bernard Wasiński ◽  
Jacek Sroka ◽  
Angelina Wójcik-Fatla ◽  
Violetta Zając ◽  
Ewa Cisak ◽  
...  
Keyword(s):  
Blood Serum ◽  
Infection Rate ◽  
Rural Community ◽  
Domestic Animals ◽  
Agglutination Test ◽  
Microscopic Agglutination Test ◽  
Serum Samples ◽  
Vistula River ◽  
The Difference ◽  
Positive Results

Abstract Blood serum samples collected from randomly selected groups of 32 pigs and 41 cows reared in farms belonging to the rural community “A” located in eastern Poland and exposed to the Vistula river floods, and serum samples from groups of 41 pigs and 40 cows from farms belonging to the rural community “B” located also in eastern Poland but not in the area exposed to floods, were examined by the microscopic agglutination test for the presence of antibodies against 18 Leptospira serovars. The percentage of serum samples presenting positive results with at least one serovar were higher in pigs and cows from community “A” comparing to community “B” (34.4% vs. 4.9% and 26.8% vs. 15.0%, respectively). In the case of pigs, the difference was statistically significant (P=0.0015). The reactions with 12 Leptospira serovars (Australis, Bataviae, Bratislava, Canicola, Hardjo, Hebdomadis, Icterohaemorrhagiae, Pomona, Poi, Cynopteri, Grippotyphosa, Celledoni,), belonging to four species (L. interrogans, L.borgpetersenii, L. kirschneri, L. weili) were found in the examined animals. In community “B”, six reactions with one serovar and two reactions with two serovars were noted whereas in community “A” - 19 reactions with one serovar, one reaction with two serovars and two reactions with six serovars were observed. The titres in animals reared in community “A” were significantly higher (up to 25,600) compared to community “B” (up to 200, P=0.0094). The obtained results suggest that the exposure to flooding may increase the infection rate in pigs and cows from afflicted areas to some extent.

scholarly journals Association of bovine uterine involution disturbances with serum neuropeptide concentrations

2020 ◽  
Vol 13 (9) ◽  
pp. 1854-1857
Author(s):  
Aida Abultdinova ◽  
Isatay Jakupov ◽  
Joachim Roth ◽  
Klaus Failing ◽  
Axel Wehrend ◽  
...  
Keyword(s):  
Blood Serum ◽  
Dairy Cows ◽  
Serum Levels ◽  
Elisa Test ◽  
Normative Values ◽  
Uterine Disease ◽  
Serum Concentrations ◽  
Serum Samples ◽  
Uterine Involution ◽  
Significant Difference

Background and Aim: Puerperal diseases influence fertility and should be diagnosed as soon as possible. This study aimed to evaluate the applicability of serum concentrations of substance P (SP), vasoactive intestinal polypeptide (VIP), and interleukin (IL)1β in the early diagnosis of uterine involution disturbances. Materials and Methods: Blood serum samples of 86 dairy cows from six different farms were harvested within the first 20 days after calving from cows with uterine involution disturbances and healthy controls, respectively. Serum concentrations for SP, VIP, and IL-1β were determined using commercially available ELISA test kits. Statistical analyses included timely changes in blood serum levels and group comparisons of healthy cows and cows with uterine disease. Results: SP concentrations increased significantly within 20 days after calving (p<0.04) with no significant difference observed between the groups. Moreover, no significant differences were found between VIP and log IL-1β. Conclusion: Results showed that none of the examined serum parameters seems suitable as indicator of uterine involution disorders. Due to the timely changes in serum concentrations of SP after calving, a correlation to diseases might not be precluded. Further research is needed as regards the establishment of normative values concerning this parameter.

scholarly journals SEROPREVALENCE OF SALMONELLA SPP. ON IMPORT BOARS FROM DENMARK TO SERBIA

2014 ◽  
Vol 6 (2) ◽  
Author(s):  
Nenad Stojanac ◽  
Ognjen Stevančević ◽  
Božidar Savić ◽  
Ivan Stančić ◽  
Aleksandar Potkonjak ◽  
...  
Keyword(s):  
Blood Serum ◽  
Cross Sectional Study ◽  
Elisa Test ◽  
Time Span ◽  
Sectional Study ◽  
Serum Samples ◽  
Salmonella Spp ◽  
Cross Sectional ◽  
One Year ◽  
Swine Herds

The sera from 120 boars from 3 farrow-to-finish swine herds in Serbia were examined for Salmonella spp. antibodies in a cross-sectional study using an ELISA test. A total of 120 blood serum samples from boars imported from Denmark were examined. All boars were seronegative to Salmonella during the import, while after one year Salmonella spp. seroprevalence ranging from 0% to 45% was found in 3 herds using two tests in the time span of one year. Only on one farm during the import in 2011, after a year boars were still seronegative. A year later on the same farm, in boars imported in 2012 the seroprevalence was 30%. Seroprevalence of Salmonella spp. in boars tested on all farms was 24,17%.

scholarly journals Association of bovine uterine involution disturbances with serum neuropeptide concentrations

2019 ◽  
Author(s):  
Aida Abultdinova ◽  
Issatay Jakupov ◽  
Joachim Roth ◽  
Klaus Failing ◽  
Axel Wehrend ◽  
...  
Keyword(s):  
Blood Serum ◽  
Dairy Cows ◽  
Serum Levels ◽  
Elisa Test ◽  
Normative Values ◽  
Uterine Disease ◽  
Serum Concentrations ◽  
Serum Samples ◽  
Uterine Involution ◽  
Significant Difference

Abstract Background: Puerperal diseases influence fertility and should be diagnosed as soon as possible. The present study aimed to evaluate the applicability of serum concentrations of substance P (SP), vasoactive intestinal polypeptide (VIP) and IL‑1β in the early diagnosis of uterine involution disturbances. Blood serum samples of 86 dairy cows from 6 different farms were harvested within the first 20 days after calving from cows with uterine involution disturbances and healthy controls, respectively. Serum concentrations for SP, VIP and IL‑1β were determined using commercially available ELISA test kits. Statistical analyses included timely changes in blood serum levels and group comparisons of healthy cows and cows with uterine disease. Results: SP-concentrations increased significantly within 20 days after calving (P < 0.04) with no significant difference between the groups. No significant differences could be shown for VIP and logIL-1β. Conclusion: None of the examined serum parameters seems suitable as indicator of uterine involution disorders. Due to the timely changes in serum concentrations of SP after calving, a correlation to diseases might not be precluded though. Further research is needed with regard to the establishment of normative values concerning this parameter.

scholarly journals Контроль эффективности вакцинопрофилактики инфекционного бронхита кур в птицеводческих хозяйствах Республики Крым

2019 ◽  
pp. 37-43
Author(s):  
S.I. Danilchenko ◽  
M.A. Pasunkina ◽  
I.B. Ionkina ◽  
N.G. Kosharnyi
Keyword(s):  
Blood Serum ◽  
Elisa Test ◽  
Serum Samples ◽  
Humoral Immune ◽  
Poultry Farms ◽  
Different Ages ◽  
The Crimea ◽  
Antibody Titers ◽  
The Republic ◽  
Production Period

Целью исследований было проведение серологического мониторинга инфекционного бронхита кур (ИБК), анализ технологических схем вакцинаций и изучение напряжённости гуморального иммунитета к вирусу ИБК. Исследования проводились в Республике Крым в течение 20172018 гг. Материалом для исследований служили образцы сывороток крови от кур разного возраста (молодняк, взрослая птица), поступивших из двух ведущих птицеводческих предприятий Крыма. Определение антител к вирусу ИБК проводили методом иммуноферментного анализа (ИФА). Всего было исследовано 1324 образца сывороток крови от птицы разного возраста. Поствакцинальные антитела обнаружены в 1317 пробах (99,4). Установлено, что применение в обследуемых хозяйствах для профилактики ИБК живых и инактивированных одно и многовалентных вакцин отечественного и импортного производства в соответствии с действующим регламентом вакцинопрофилактики и разработанными в хозяйствах технологическими схемами вакцинации создаёт полноценную специфическую защиту у вакцинированного поголовья на протяжении всего периода продуктивности. Отмечено, что постепенное нарастание средних титров антител у птиц каждой следующей возрастной группы с пиком в 140 и 150дневном возрасте соотносится с классической схемой формирования гуморального иммунитета при применении живых и инактивированных вакцин. В дальнейшем у кур старше 300суточного возраста появляются достаточно пёстрые титры антител к ИБК, что может свидетельствовать о возможной циркуляции полевого вируса ИБК. В связи с этим имеется необходимость систематического мониторинга уровня антител к вирусу ИБК, что позволит и дальше контролировать эпизоотическую ситуацию к данному заболеванию в птицеводческих хозяйствах Крыма.The aim of the research was to conduct serological monitoring of avian infectious bronchitis (AIB), the analysis of technological schemes of vaccinations and the study of the intensity of humoral immune response to the AIB virus. The researches were carried out in the Republic of Crimea during 20172018. The material for the researches were samples of blood serum from chickens of different ages (young birds, mature birds) received from two leading poultry farms of the Crimea. Determination of antibodies to AIB virus was conducted by enzyme multipled immunoassay (ELISA test). A total of 1324 blood serum samples from birds of different ages were examined. Vaccineinduced antibodies were found in 1317 samples (99.4). It has been established that the use of live and inactivate monovalent and multivalent vaccines of domestic and foreign production in the surveyed farms for the prevention of AIB in accordance with the current vaccinal prevention schedule and the vaccination technological schemes developed on the farms creates full specific protection for the vaccinated livestock throughout the entire production period. It is noted that a gradual increase in the average antibody titers in birds of each subsequent age group with a peak at 140 and 150 days of age correlates with the classical scheme for the formation of antibody mediated immunity with the use of live and inactivate vaccines. Further chickens older than 300 days of age appear to have rather variegated antibody titers to AIB which may indicate a possible circulation of the field AIB virus. In this regard there is a need for systematic monitoring of the level of antibodies to the AIB virus which will allow further control of the epizootic situation to this disease in the poultry farms of the Crimea.

scholarly journals Study of leptospirosia in animal and livestock

2019 ◽  
Vol 26 (01) ◽  
pp. 34-41
Author(s):  
Enkhtsetseg N ◽  
Altankhuu B ◽  
Narangerel B
Keyword(s):  
Infection Rate ◽  
Bovine Serum ◽  
Urban Slum ◽  
Elisa Test ◽  
Enzyme Linked Immunosorbent Assay ◽  
Bacterial Disease ◽  
Serum Samples ◽  
Slum Dwellers ◽  
Swine Serum ◽  
Sera Samples

Leptospirosis is a zoonotic bacterial disease that affects vulnerable populations such as rural subsistence farmers and urban slum dwellers. Leptospirosis is an infectious disease caused by pathogenic organisms belonging to the genus leptospira, that are transmitted directly or indirectly from animals to humans. In Mongolian human cases of leptospirosis has not been reported yet and this zoonotic disease not well study. We had use the kit in order to detect Pomona, Tarassovi, Hebdomadis, Icterohaemorrhagiae, Grippotyphosa, Sejroe and Canicola serotype by Enzyme-linked immunosorbent assay (ELISA) test. We analyzed 397 samples of bovine serum by indirect ELISA. The bovine serum samples were collected from Selenge province, and out of 101 samples 2 were positive. And, out of 171 bovine 1 serum was positive in Tov province. In contrast, no positive samples detected in 137 swine serum. Linnodee leptospira /ireland/- ELISA kit can detect a swine Bratislava serotype and cattle Hardjo serotype. To detect Hardjo serotype, 397 samples of cattle serum and 137 samples of swine serum were investigated. The 29 serum of cattle were positive, which has 7.3% infection rate, and, only one swine sera was positive out of 137, that has 0.73% infection rate. The 137 swine serum were tested by ELISA, which can detect Leptospira Bratislava serotype; and 12 out of that were positive. This indicates 8.7% of all sample are positive. Total of 397 bovine sera were examined by ELISA and specific antibody against Pomona, Tarassovi, Hebdomadis, Icterohaemorrhagiae, Grippotyphosa, Sejroe and Canicola serotype detected in 0,75 % (3 sera samples). Hardjo serotype detected in 7.3 % (29 sera) of bovine samples and 7.6 % (1 serum) of 13 swine sera samples and furthermore, bratislava serotype antibody detected in 8.7% (12 sera) of the pigs included in our study. Our study indicates that risk of human leptospirosis infection through animal derived food consumption, soil and water contamination is present due to prevalence of hardjo and bratislava serotype in cattle and pig farms. These results correlates with study conducted by Odontsetseg N. PhD in 2005 which stated that Hardjo serotype of Leptospira interrogans was detected in cattle herd in our country and these suggest that leptospirosis is prevalent in certain regions of our country. Мал амьтны лептоспирозийн тандан судалгааны дүн Хураангуй:  Мал амьтны гаралтай хүнсний бүтээгдэхүүн ус, хөрсөөр дамжин хүнд халдварладаг зооноз өвчин болох Лептоспирозийн танадан судалгааг Булган, Орхон, Сэлэнгэ, Төв аймгууд болон Улаанбаатар хот орчмоос цуглуулсан үхрийн 721, гахайн 169, зарим мэрэгч амьтдын 108, усны 22 нийт 1020 сорьцыг ийлдэс судлал болон молекул биологи, нян судлалын аргаар шинжлэв. Судалгааны дүнд шинжилгээнд хамрагдсан нийт 397 үхрийн сорьцны 32 буюу 8.06%, 137 гахайн сорьцны 13 буюу 9.4%-д нь L. pomona, L. tarassovi, L. hebdomadis, L. icterohaemorrhagiae, L. grippotyphosa, L. sejroe, L. canicola, L. hardjo, L. bratislava хэвшлүүдийн эсрэг үүссэн өвөрмөц эсрэгбием тус тус илрэв. Лептоспирозийн байгалийн дамжуулагч болох мэрэгчийн 108-н сорьцонд Полимериазан Гинжин Урвал (ПГУ) тавихад 8 сорьц буюу 7.4%-д нь лептоспирозийн G1, G2 генийн өвөрмөц бүтээгдэхүүн илэрсэн болно. Ийлдэс судлал, ПГУ- аар эерэг гарсан сорьцуудад үүсгэгч илрүүлэх нян судлалын шинжилгээ хийсэн боловч үүсгэгч өсгөвөрлөгдсөнгүй. Манай орны Орхон, Сэлэнгийн сав газар, Архангай, Төв аймаг, Улаанбаатар хот орчмын үхэр, гахай, мэрэгчидийн сорьц лептоспирозоор эерэг дүн үзүүлсэн нь манай орны мал амьтан, мэрэгчидэд өвчний халдварлалт байгааг харуулж байна. Түлхүүр үг: Leptospira, үүсгэгч, өвөрмөц эсрэгбием, ийлдсийн хэвшил, ген

scholarly journals First report of bovine rotavirus and bovine coronavirus seroprevalance in goats in Turkey

2007 ◽  
Vol 61 (1-2) ◽  
pp. 75-79 ◽  
Author(s):  
Okur Gumusova ◽  
Z. Yazici ◽  
H. Albayrak ◽  
D. Çakiroglu
Keyword(s):  
Blood Serum ◽  
Serum Samples ◽  
Bovine Coronavirus ◽  
First Report ◽  
Bovine Rotavirus ◽  
Goat Population ◽  
First Time

In this study, bovine coronavirus (BCV) and bovine rotavirus (BRV) seroprevalances were detected by ELISA in 107 goat blood serum samples obtained from five different provinces of Northern Turkey. The results of the study reflected 41.12% and 82.24% seropositivity against BCV and BRV, respectively, in the goat sera. BCV seroprevalance in mature goats is determined for the first time with this study. Furthermore, this is the first statement of BRV and BCV seroprevalances in the mature goat population in Turkey.

scholarly journals STUDY OF ELISA TEST-SYSTEMS OF DIFFERENT FORMATS FOR DETECTION OF MEASLES VIRUS SPECIFIC IgM IN DIFFERENT GEOGRAPHIC ZONES

2018 ◽  
Vol 8 (2) ◽  
pp. 230-234
Author(s):  
M. A. Bichurina ◽  
N. V. Zheleznova ◽  
I. N. Lavrentieva ◽  
A. Yu. Antipova ◽  
L. B. Kulyashova ◽  
...  
Keyword(s):  
Blood Serum ◽  
False Positive ◽  
Measles Virus ◽  
Test System ◽  
Elisa Test ◽  
Healthy Adults ◽  
Igg Antibodies ◽  
Serum Samples ◽  
Test Systems ◽  
Igm Antibodies

Detection of the measles virus (MV) specific IgM antibodies in blood serum of patients is considered to be the main standard for the  laboratory confirmation of measles diagnosis, the test being  acknowledged by WHO. As it was demonstrated earlier the specific  IgM antibodies as the marker of the acute MV infection were  detected in 97.2–100% of blood serum samples from patients using  the ELISA test-systems of the “capture” format (Microimmun Ltd.  and Vector Best). In case when the ELISA test-system of the  “indirect” format (Siemens, Germany) was used only 63.9% of these sera turned to be IgM positive. And on the contrary using the  “indirect” format ELISA test-system Euroimmun, Germany, for  detection of the MV specific IgM the false positive results were  obtained.The aim of the study was the comparative evaluation of  the different format ELISA test-systems used for the detection of the  MV specific IgM antibodies in blood sera of patients and healthy  adults collected in different geographic zones.Materials and  methods. In total 108 serum specimens collected in 2015–2017 were studied: from healthy adult Guineans, residents of the Republic of Guinea (RG); patients aged 1–70 with the initial  “infectious mononucleosis”, “infectious cytomegalovirus” and  “rubella” diagnosis and taken from the bank of sera in the  Subnational Measles/Rubella laboratory, StP Measles/Rubella RC in  NWFR. The MV specific IgM antibodies were detected using the commercial ELISA test-systems “VectoMeasles-IgM” (Vector-Best,  Russia) (“capture” format) and “Anti-Measles Virus ELISA IgM (NP)”  (Euroimmun Medizinische Labordiagnostik AG, Germany) («indirect»  format). The specific Epshtein-Barr Virus (EBV) IgM and IgG  antibodies were detected with the commercial ELISA test-systems «DS-ELISA-anti-EBV-VCA-M», «DS-ELISA-anti-EBV-EA-G» and «DS-ELISA-anti-EBV-NA-G» (“Diagnostic Systems”, Russia).Results and discussion. The MV specific IgM antibodies were not revealed in the total of 108 blood serum samples from the healthy adults and patients, residents of the Russia and of the RG, with the “capture”  format “VectoMeasles-IgM” ELISA test-system. The absence of the  acute MV infection was also confirmed by the high measles immunity  level (i.e. IgG MV antibodies titers) as well as by detection of the IgG antibodies of high avidity. At the same time in 6  of 108 total sera (5.5%) IgM MV antibodies were detected with the «indirect» format ELISA test system Euroimmun, Germany. In these 6 sera the EBV specific antibodies were also evidenced. The results  obtained demonstrate the nonspecific reaction due to the possible  reactivity with anti-EBV antibodies. Besides this the different  percentage of the false positive reactions in sera from healthy  adults, residents of the RG and residents of Russia was determined  — 8.5±4.0% and 3.2±2.2% correspondently. Thus the preliminary  results, and to get the final results for general conclusions increase  of the total amount of the clinical specimens under studying is of extremely importance.

scholarly journals Screening of SRLV in herds in mid-Eastern Poland

2018 ◽  
Vol 74 (8) ◽  
pp. 532-535
Author(s):  
WIKTOR BOJAR ◽  
ANDRZEJ JUNKUSZEW ◽  
MONIKA OLECH ◽  
JACEK KUŹMAK ◽  
KLAUDIUSZ SZCZEPANIAK
Keyword(s):  
Blood Serum ◽  
Detailed Analysis ◽  
Elisa Test ◽  
Serological Response ◽  
Flock Size ◽  
Serological Analysis ◽  
Serum Samples ◽  
Specific Antibodies ◽  
Merino Sheep ◽  
Detailed Diagnosis

The aim of the study was to evaluate the epidemiological condition of SRLV infections in nucleus flocks of sheep. The surveillance of Lentivirus infections was conducted in sheep flocks from the mid-Eastern part of Poland: the area of Lubelskie, Świętokrzyskie and Podkarpacie provinces. Ninety eight flocks were chosen for a detailed analysis. They included 6,470 mother ewes belonging to 15 breeds and lines: Polish Merino sheep, Polish Lowland sheep, Wielkopolska sheep, Żelaźnieńska sheep, Uhruska sheep, Olkuska sheep, Polish Heath sheep, Świniarka sheep, Polish Pogorze sheep, Podhale Zackel sheep, Black-headed sheep, Ile de France, Berrichon du Cher, BCP and SCP. The identification of the infected animals and the assessment of the epidemiological condition of the flocks was carried out using a serological analysis of blood serum samples. The assay of MVV-specific antibodies in blood serum was performed using the ELISA test. The results obtained showed that the highest rate of flocks with at least one animal with a positive serological response was in the Podkarpacie Province (71.43%), and the lowest rate (27.03%) was observed in Lubelskie Province. The analysis of the rate of the sheep infected with SRLV indicated that the greatest number of seropositive animals was highest in Podkarpackie Province (19.9%), which corresponds to the results for the flocks, and was significantly higher (p ≤ 0.01) than in Świętokrzyskie Province (11.35%) and Lubelskie Province (9.20%). The flock size turned out to be a factor which significantly influenced the prevalence of SRLV infections. The prevalence was highest (13.6%) in the flocks exceeding 100 sheep, and decreased together with the decreasing number of heads in the flock. It must be stressed that the problem of SRLV infections is very complex and depends on many factors. Therefore it is necessary to carry out a detailed diagnosis of the factors leading to expansion of the virus in sheep flocks before developing flock protection programs...

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