scholarly journals Spectrophotometric analysis of phosphate concentration in agricultural soil samples and water samples using molybdenum blue method

2016 ◽  
Vol 3 (6) ◽  
pp. 407-412
Author(s):  
Funmilayo Enitan Adelowo ◽  
Samuel O. Agele
Keyword(s):  
Spectrophotometric Method ◽  
Water Samples ◽  
Agricultural Soil ◽  
Spectrophotometric Analysis ◽  
Soil Samples ◽  
Phosphate Concentration ◽  
Molybdenum Blue ◽  
Dissolved Phosphorus ◽  
Uv Visible ◽  
Soil And Water

Phosphorus has a strong affinity for soil thereby increasing the rate of dissolved phosphorus that will be transported in runoff. The most important contributing factor for increasing in phosphates levels are natural and human factors such as dumping of refuse from nearby areas, use of fertilizers and human excretes. The phosphate levels in the soil and water samples (expressed in part per million) was analyzed by molybdenum blue phosphorus method using a simple analytical and UV-visible spectrophotometric method and the phosphates level was measured at 740 nm. The calibration curve was formed using concentrations of 0.5-3.0 mg/L. The samples analyzed showed high levels of phosphates which could be due to domestic, natural and agricultural factors.

scholarly journals UV SPECTROPHOTOMETRIC ANALYSIS AND VALIDATION OF OSELTAMIVIRPHOSPHATE IN PURE AND PHARMACEUTICAL FORMULATION

2020 ◽  
pp. 111-114 ◽  
Author(s):  
SACHIN A. YANJANE ◽  
SHRISHAIL M. GHURGHURE ◽  
VINOD K. MATOLE
Keyword(s):  
Spectrophotometric Method ◽  
Spectrophotometric Analysis ◽  
Pharmaceutical Formulation ◽  
Ich Guidelines ◽  
Highly Sensitive ◽  
Double Beam ◽  
Recovery Study ◽  
Uv Visible ◽  
Oseltamivir Phosphate ◽  
Simple Economic

Objective: A new, simple, economical, precise, sensitive, linear, accurate, rapid UV spectrophotometric method has been developed for the estimation of Oseltamivir Phosphate in pure form and pharmaceutical formulation. Methods: This UV method was developed using Methanol as a solvent. In the present method, the wavelength selected for analysis was 218 nm. UV-Visible double beam spectrophotometer (Systronic 2201) was used to carry out spectral analysis. The ICH guidelines were used to validate the method. Results: The method was validated for linearity, range, accuracy, precision, robustness, LOD and LOQ. Linearity was found in the range of 10-50µg/ml. Accuracy was performed by using a recovery study. The amount of drug recovered was found to be in the range of 99.01-100.1%. The % RSD value was found to be less than 2. Conclusion: The developed UV spectrophotometric method was found to be simple, economic, sensitive, easy, accurate, linear, specific and highly sensitive and can be used for routine estimation of Oseltamivir Phosphate.

scholarly journals A SIMPLE SPECTROPHOTOMETRIC METHOD FOR THE QUANTITATIVE ANALYSIS OF PHOSPHATE IN THE WATER SAMPLES

2018 ◽  
Vol 7 (2) ◽  
pp. 198
Author(s):  
Nur Habibah ◽  
I Gusti Ayu Shri Dhyanaputri ◽  
I Wayan Karta ◽  
Cokorda Dewi Widhya Hana Sundari ◽  
Mochammad Choirul Hadi
Keyword(s):  
Quantitative Analysis ◽  
Spectrophotometric Method ◽  
Water Samples ◽  
Calibration Graph ◽  
Ammonium Molybdate ◽  
Official Method ◽  
Phosphate Content ◽  
Color Intensity ◽  
Molybdenum Blue ◽  
Sulfuric Acid Medium

A simple spectrophotometric method was used to determine the phosphate content in the water sampels. The method is based on the formation of molybdenum blue complex from the reaction of orthophosphate and ammonium molybdate followed by reduction with ascorbic acid in the aqueous sulfuric acid medium. The color intensity of the molybdenum blue complex is proportionally to the phosphate content in the solution. The system obeys Lamber-Beer’s Law at the 890 nm in the concentration range of 0.1-1 mg P/L and the linier calibration graph was obtained with the slope, 0.6334, intercept, 0.0074 and correlation coefficient of 0.9988. This official method was also used for the quantitative analysis of phosphate in the water samples and the satisfactory result was obtained. The range of phosphate concentration in the water samples are 0.033 – 2.943 mg P/L.

scholarly journals Diversity ofLeptospiraspp. in Rats and Environment from Urban Areas of Sarawak, Malaysia

2017 ◽  
Vol 2017 ◽  
pp. 1-8 ◽  
Author(s):  
Chai Fung Pui ◽  
Lesley Maurice Bilung ◽  
Kasing Apun ◽  
Lela Su’ut
Keyword(s):  
Polymerase Chain Reaction ◽  
Water Samples ◽  
Urban Areas ◽  
Environmental Samples ◽  
Soil Samples ◽  
Chain Reaction ◽  
Prevalence Studies ◽  
Pcr Method ◽  
Polymerase Chain ◽  
Soil And Water

Various prevalence studies onLeptospirain animals and humans, as well as environmental samples, had been conducted worldwide, including Malaysia. However, limited studies have been documented on the presence of pathogenic, intermediate, and saprophyticLeptospirain selected animals and environments. This study was therefore conducted to detectLeptospiraspp. in rats, soil, and water from urban areas of Sarawak using the polymerase chain reaction (PCR) method. A total of 107 rats, 292 soil samples, and 324 water samples were collected from April 2014 to February 2015. PathogenicLeptospirawas present in 5.6% (6/107) of rats, 11.6% (34/292) of soil samples, and 1.9% (6/324) of water samples. IntermediateLeptospirawas present in 2.7% (8/292) of soil samples and 1.9% (6/324) of water samples. SaprophyticLeptospirawas present in 10.3% (11/107) of rats, 1.4% (4/292) of soil samples, and 0.3% (1/324) of water samples. From this study, 76Leptospiraspp. were isolated. Based on DNA sequencing, the dominantLeptospiraspp. circulating in urban areas of Sarawak are pathogenicLeptospira noguchii, intermediateLeptospira wolffiiserovar Khorat, and saprophyticLeptospira meyeri, respectively. Overall, this study provided important surveillance data on the prevalence ofLeptospiraspp. from rats and the environment, with dominant local serovars in urban areas of Sarawak.

scholarly journals DEVELOPMENT AND VALIDATION OF UV-VISIBLE SPECTROPHOTOMETRIC METHOD FOR DETERMINATION OF DULOXETINE

2019 ◽  
pp. 27-31
Author(s):  
LIPSA SAMAL ◽  
AMARESH PRUSTY
Keyword(s):  
Spectrophotometric Method ◽  
Spectroscopic Method ◽  
Solvent System ◽  
Spectrophotometric Analysis ◽  
Thiophene Derivative ◽  
Experimental Conditions ◽  
Relative Standard ◽  
Uv Visible ◽  
Development And Validation

Objective: The aim of the present work was to develop and validate a simple UV spectroscopic method for the determination of duloxetine, which is a thiophene derivative and a selective neurotransmitter reuptake inhibitor for serotonin, norepinephrine, and to lesser degree dopamine. Methods: The UV Spectrophotometric analysis was performed using Shimadzu UV-1800 and Shimadzu UV-1700 spectrophotometer by using solvent system acetonitrile and water in the ratio of 8:2. Detection was performed at a wavelength of 290 nm. Method validation was carried out according to ICH Q2R1 guidelines by taking the parameters linearity, accuracy, precision, ruggedness, and robustness, LOD and LOQ. Results: The UV Spectrophotometric method was found linear in the range of 10-50 μg/ml. The method was rugged and robust with % relative standard deviation less than 2. The extraction recoveries were found to be higher than 99% in all experimental conditions. Conclusion: Based upon the performance characteristics, the proposed method was found accurate, precise and rapid and suitable for the determination of Duloxetine for routine analysis.

Assessment of arsenic contamination in soils and waters in some areas of Bangladesh

2000 ◽  
Vol 42 (7-8) ◽  
pp. 185-192 ◽  
Author(s):  
M. B. Alam ◽  
M. A. Sattar
Keyword(s):  
Water Samples ◽  
Arsenic Contamination ◽  
Soil Samples ◽  
Arsenic Content ◽  
Molybdenum Blue

The soil samples and tubewell waters were collected from 25 locations representing five thanas of four districts of Bangladesh. The soils were collected from three depths viz. 0–15, 15–30 and 30–45 cm and tubewell waters were collected from same locations. The arsenic content of soils and waters were detected by Molybdenum blue method. The arsenic content in soils ranged from 1.27–56.68, 3.18–54.77, 1.27–50.95, 1.27–39.48 and 3.18–35.66 ppm in Chapainawabganj Sadar, Kustia Sadar, Bera, Ishurdi and Sarishabari thanas, respectively. Out of a total of 25 samples arsenic was detectable for 18 samples at 0–15 cm, 17 samples at 15–30 cm and 15 samples at 30–45 cm depth. One sample at 0–15 cm, 7 samples at 15–30 cm and 4 samples at 30–45 cm depth were found to be slightly contaminated. In tubewell water the arsenic content measured from Chapainawabganj Sadar, Kustia Sadar, Bera, Ishurdi and Sarishabari thanas were ranged 0.010–0.056, 0.010–0.071, 0.010–0.056, 0.010–0.056 and 0.025–0.071 ppm, respectively. Out of 25 water samples 17 contained variable amounts of arsenic where 6 sampling sites contained arsenic levels above 0.05 ppm, and these sites are Rajarampur of Chapainawabganj Sadar thana, Jordaha of Bera thana, Courtpara of Kustia Sadar thana, Nalgari of Ishurdi thana and Ijarapara of Sarisabari thana. Arsenic contained in soils was positively correlated with arsenic content in waters.

scholarly journals Synthetic Pyrethroids Pesticide Residues in Soils and Drinking Water Sources from Cocoa Farms in Ghana

2016 ◽  
Vol 5 (1) ◽  
pp. 60 ◽  
Author(s):  
Benedicta Y. Fosu-Mensah ◽  
Elvis D. Okoffo ◽  
Michael Mensah
Keyword(s):  
Drinking Water ◽  
Water Samples ◽  
Pesticide Residues ◽  
Agricultural Soils ◽  
Water Source ◽  
Soil Samples ◽  
Synthetic Pyrethroids ◽  
Lambda Cyhalothrin ◽  
Cocoa Farm ◽  
Soil And Water

The contamination of pesticides in 32 soils and 64 drinking water samples was investigated from cocoa farms in the Dormaa West District of Ghana to assess pollution status. A total of nine synthetic pyrethroids pesticides were measured with a high resolution Varian CP-3800 Gas Chromatograph equipped with <sup>63</sup>Ni electron capture detector (ECD). Eight synthetic pyrethroid residues namely fenvalerate, deltamethrin, cypermethrin, bifenthrin, permethrin, lambda-cyhalothrin, allethrin and cyfluthrin were detected with lambda-cyhalothrin and allethrin occurring most frequently in soil and water respectively. The concentrations of synthetic pyrethroids residues in the soil samples were in the ranges of; 0.02-0.03 mg/kg for lambda-cyhalothrin, 0.010-0.02 mg/kg for allethrin, 0.010-0.04 mg/kg for cyfluthrin, &lt;0.01-0.04 mg/kg for cypermethrin, 0.02-0.06 mg/kg for deltamethrin, and &lt;0.01-0.03 mg/kg for bifenthrin. Similarly, the synthetic pyrethroids residues in the water samples were in the ranges of; 0.01-0.05 µg/L for allethrin, 0.01-0.04 µg/L for fenvalerate, 0.01-0.04 µg/L for cypermethrin and 0.01-0.05 µg/L for deltamethrin. The concentrations of synthetic pyrethroids pesticide residues recorded in the soil samples analysed were generally below and within their respective US MRLs for agricultural soils, except the mean concentration values recorded for pesticides such as lambda-cyhalothrin at Diabaa (S2) and Krakrom (S3), allethrin at Diabaa (S2) and deltamethrin at Kwakuanya (S4), which were above their respective US MRLs for agricultural soils. The trends of synthetic pyrethroids pesticide residues in the water samples analysed from the various distances to cocoa farms decreased with an increase of water source to cocoa farm (ranking; 0-15m&gt;16-30m&gt;above 30m). All synthetic pyrethroids pesticide residues recorded in the water samples were below and within their respective WHO MRLs for drinking water except for deltamethrin, which exceeded the WHO MRL at Kwakuanya (S4) at distance 0-15m from a cocoa farm. The presence of synthetic pyrethroids residues in the soil and water samples analysed is an indication of the use of the pesticide by cocoa farmers in the study area. The routine monitoring of pesticide residues in the study area is necessary for the control and reduction of environmental pollution.

scholarly journals Study on Arsenic Contamination in Soil and Water in Five-selected Agro Ecological Zone (AEZ) of Bangladesh

2010 ◽  
pp. 137-142
Author(s):  
Md Nurul Huda-Al Mamun
Keyword(s):  
Water Samples ◽  
Test Tube ◽  
Soil Samples ◽  
Well Water ◽  
Ecological Zone ◽  
Toxic Level ◽  
Ecological Zones ◽  
Tube Wells ◽  
Soil And Water ◽  
Agro Ecological Zones

A research was carried out at the five different Agro-Ecological Zones (AEZ) of Bangladesh, viz. Tista Meander Floodplain (FP), High Ganges FP, Low Ganges FP, Active Ganges FP and Gopalganj-Khulna Bills during January 2001 to January 2002. The objectives were to assess the arsenic (As) concentration in the soil and water as well as to test tube well water samples and examines whether these exceed the acceptable limit of  As. Water of shallow tube wells (STW) and soil samples were collected from ten sites under five AEZs of Bangladesh. Considerable number of water samples showed high contents of arsenic, which ranged from 0.032 to 203.9 ?g/l. Out of 428 water samples, 155 were found unsafe (>10-<50 ?g/l) and 78 were toxic (>50 ?g/l) for drinking purpose. However, out of 2648 soil samples collected from different Upazilas, only 4 samples collected from Chargat showed toxic level (>20 ?g/kg).

scholarly journals Intestinal parasitoses and environmental factors in a rural population of Argentina, 2002-2003

2007 ◽  
Vol 49 (4) ◽  
pp. 251-255 ◽  
Author(s):  
Juan A. Basualdo ◽  
María A. Córdoba ◽  
María M. de Luca ◽  
María L. Ciarmela ◽  
Betina C. Pezzani ◽  
...  
Keyword(s):  
Water Samples ◽  
Giardia Lamblia ◽  
Environmental Variables ◽  
Intestinal Parasites ◽  
Soil Samples ◽  
Water Pump ◽  
Blastocystis Hominis ◽  
Degree Of Association ◽  
Sanitary Conditions ◽  
Soil And Water

We evaluate the prevalence of intestinal parasites in 504 people and the degree of association between environmental variables and parasites found in population, soil and water in a rural area of Argentina during 2002-2003. A structured survey was used to evaluate the environmental variables and fecal-human, soil and water samples were analyzed. The prevalence of parasites was 45.4%. Most prevalent protozoa were Blastocystis hominis (27.2%) and Giardia lamblia (6.9%), while the most prevalent helminth was Ascaris lumbricoides (3.8%). The analyzed environmental variables showing association (p < 0.05) with presence of parasites in population were: cardboard-tin or wooden house, dirt floor, home or communal water pump, faucet outside the house or public faucet and cesspool or latrine. Parasite forms were found in 82.3% of the soil samples and in 84.2% of the water samples. In both samples we found parasites that were also found in people. In this study we have found deficient sanitary conditions associated with presence of parasites in population and we have evidenced that contaminated soil and water were the source of these parasites.

scholarly journals Environmental Health Risk Analysis of Paraquat Exposure in Palm Oil Plantations

2016 ◽  
Vol 5 (4) ◽  
pp. 465 ◽  
Author(s):  
Maksuk Maksuk ◽  
Tan Malaka ◽  
Suheryanto Suheryanto ◽  
Abu Umayah
Keyword(s):  
Body Weight ◽  
Palm Oil ◽  
Water Samples ◽  
Laboratory Tests ◽  
Soil Samples ◽  
Risk Quotient ◽  
Cross Sectional ◽  
Grab Sampling ◽  
Environmental Media ◽  
Soil And Water

Paraquat (1, 1’-dimethyl-4, 4’-bipyridylium dichloride) is a highly toxic herbicide. Recently, Paraquat is still used widely in the plantations area, mainly in palm oil plantations. Paraquat application continuously can be increased Paraquat concentration and contaminated in soil and water. This study aimed was to analysis Paraquat exposure in environmental media water and soil, and the calculation of risk quotient of Paraquat in palm oil plantation. The study design of this research was a cross sectional with the laboratory examination Paraquat concentrations in soil and water. Soil and water samples taken by grab sampling with random composites. Water samples were taken from three sources in palm oil plantations area. Whereas soil samples were taken at a depth of 0-10 cm (top soil) and the number of soil samples as much as 5 samples. Furthermore, the risk quotient of Paraquat concentration in the water used in the palm oil plantation. The laboratory tests of Paraquat concentration were in water and soil with gas chromatography. The results of laboratory tests showed that Paraquat concentration in water samples were found between &lt;0.005 to 0.01 mg/l and Paraquat concentration in soil samples were found between &lt;0.005 to 0.08 ppm. The calculation of risk quotient was 0.058 mg/body weight/day. Although, the calculated of risk quotient showed less than one. Therefore, prior to use the water must be treatment first. While the recommended water to consumption as much as 2 liters/day for a 55 kg body weight to Indonesian workers.

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