scholarly journals Thin-layer chromatographic analysis of flavonoids and total phenolics in methanolic and ethanolic extracts of Senna alata (L.) Roxb. (Fabales: Fabaceae)

2016 ◽  
Vol 3 (5) ◽  
pp. 221
Author(s):  
Solomon Oluwole Oladeji
Keyword(s):  
Thin Layer ◽  
Gallic Acid ◽  
Chromatographic Analysis ◽  
Antibacterial Activities ◽  
Total Phenolic ◽  
Antimicrobial Substances ◽  
Soxhlet Apparatus ◽  
Ethanolic Extracts ◽  
Thin Layer Chromatographic Analysis ◽  
Senna Alata

Herbal medicine has proved as a major source of therapeutic agents. They have been used to combat bacterial and fungal related infections and diseases. People depend on medicinal plants such as Senna alata (L.) Roxb. (Fabales: Fabaceae) without the knowledge of the chemical composition of the plant. Therefore, there is a need to examine the bioactive components that are present in S. alata. The methanolic and ethanolic extracts were obtained using soxhlet apparatus and the concentrated extracts were purified using column chromatography. The fractions were eluted and investigated for the presence of total flavonoids and phenolics. The values of thin-layer chromatographic analysis for the leaf, flower and fruit extracts of S. alata when compared to gallic acid and quercetrin (standard) showed that the fractions contained a chemical component in the class of gallic acid and quercetrin, respectively. The presence of total phenolic and flavonoids could be responsible for the observed antifungal and antibacterial activities of the plant. It could be concluded that this plant is a natural source of antimicrobial substances of high importance and are useful in antiseptic and disinfectant formulation as well as in chemotherapy.

scholarly journals Gas chromatographic and antimicrobial analyses of phenolic compounds in Senna alata (L.) Roxb. (Fabales: Fabaceae)

2016 ◽  
Vol 3 (6) ◽  
pp. 355-365
Author(s):  
Funmilayo Adelowo ◽  
Oluwole Oladeji
Keyword(s):  
Gas Chromatography ◽  
Phenolic Compounds ◽  
Antibacterial Activities ◽  
Bioactive Metabolites ◽  
Diffusion Method ◽  
Chemical Components ◽  
Bioactive Components ◽  
Antimicrobial Substances ◽  
Agar Disc ◽  
Senna Alata

This present work is aimed at determining the chemical components in Senna alata (L.) Roxb. (Fabales: Fabaceae), that contains a large array of phenolic compounds which make it useful as antifungal and antibacterial. Despite a notable success in the recent researches, yet, the quantification of the chemical components has not been actually specified. There is a claim by local people that S. alata can cure fungi and bacteria related diseases. Therefore, there is a need to ascertain this claim by examining the bioactive components present in the plant. The Gas Chromatography (GC) analysis was performed using a Clarus 500 Perkin Elmer gas chromatography equipped with an Elite-5 capillary column with the addition of an absolute alcohol. The antimicrobial analysis was carried out using Agar Disc Diffusion Method. In the GC chromatogram, four prominent peaks were observed in leaf chromatogram, three peaks in flower chromatogram and five peaks in fruit chromatogram, respectively. The flower contained the highest amount of cinnamic acid (52.91%) and the fruit contained the least (17.03%). The presence of some bioactive metabolites could be responsible for the observed antifungal and antibacterial activities on the susceptible organisms studied. It could be concluded that this plant is natural sources of antimicrobial substances of high importance and are useful in chemotherapy.

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