scholarly journals Up-regulation of brain-derived neurotrophic factor in the dorsal root ganglion of the rat bone cancer pain model

2014 ◽  
pp. 415 ◽  
Author(s):  
Naoto Tomotsuka ◽  
Ryuji Kaku ◽  
Norihiko Obata ◽  
Yoshikazu Matsuoka ◽  
Hirotaka Kanzaki ◽  
...  
Keyword(s):  
Dorsal Root Ganglion ◽  
Cancer Pain ◽  
Neurotrophic Factor ◽  
Dorsal Root ◽  
Bone Cancer ◽  
Brain Derived Neurotrophic Factor ◽  
Bone Cancer Pain ◽  
Pain Model ◽  
Rat Bone

scholarly journals Radiotherapy Suppresses Bone Cancer Pain through Inhibiting Activation of cAMP Signaling in Rat Dorsal Root Ganglion and Spinal Cord

2016 ◽  
Vol 2016 ◽  
pp. 1-8 ◽  
Author(s):  
Guiqin Zhu ◽  
Yanbin Dong ◽  
Xueming He ◽  
Ping Zhao ◽  
Aixing Yang ◽  
...  
Keyword(s):  
Spinal Cord ◽  
Dorsal Root Ganglion ◽  
Cancer Pain ◽  
Signaling Pathway ◽  
Dorsal Root ◽  
Mechanical Allodynia ◽  
Thermal Hyperalgesia ◽  
Bone Cancer ◽  
Bone Cancer Pain ◽  
Sprague Dawley

Radiotherapy is one of the major clinical approaches for treatment of bone cancer pain. Activation of cAMP-PKA signaling pathway plays important roles in bone cancer pain. Here, we examined the effects of radiotherapy on bone cancer pain and accompanying abnormal activation of cAMP-PKA signaling. Female Sprague-Dawley rats were used and received tumor cell implantation (TCI) in rat tibia (TCI cancer pain model). Some of the rats that previously received TCI treatment were treated with X-ray radiation (radiotherapy). Thermal hyperalgesia and mechanical allodynia were measured and used for evaluating level of pain caused by TCI treatment. PKA mRNA expression in dorsal root ganglion (DRG) was detected by RT-PCR. Concentrations of cAMP, IL-1β, and TNF-αas well as PKA activity in DRG and the spinal cord were measured by ELISA. The results showed that radiotherapy significantly suppressed TCI-induced thermal hyperalgesia and mechanical allodynia. The level of PKA mRNA in DRG, cAMP concentration and PKA activity in DRG and in the spinal cord, and concentrations of IL-1βand TNF-αin the spinal cord were significantly reduced by radiotherapy. In addition, radiotherapy also reduced TCI-induced bone loss. These findings suggest that radiotherapy may suppress bone cancer pain through inhibition of activation of cAMP-PKA signaling pathway in DRG and the spinal cord.

Sign in / Sign up

Export Citation Format

Share Document