scholarly journals Poly(n-butylcyanoacrylate) nanoparticles for oral delivery of quercetin: preparation, characterization, and pharmacokinetics and biodistribution studies in Wistar rats

2015 ◽  
pp. 3921 ◽  
Author(s):  
Zaved Khan ◽  
Mayur Bagad
Keyword(s):  
Oral Delivery ◽  
Wistar Rats ◽  
Biodistribution Studies

Genotoxicity, biochemical, and biodistribution studies of magnesium oxide nano and microparticles in albino wistar rats after 28-day repeated oral exposure

2017 ◽  
Vol 33 (4) ◽  
pp. 396-410 ◽  
Author(s):  
Bhanuramya Mangalampalli ◽  
Naresh Dumala ◽  
Rekhadevi Perumalla Venkata ◽  
Paramjit Grover
Keyword(s):  
Magnesium Oxide ◽  
Wistar Rats ◽  
Oral Exposure ◽  
Biodistribution Studies

scholarly journals Ciprofloxacin loaded castor oil based emulsion: Its antimicrobial, hematotoxicity and soft tissue pathophysiological study in Wister rats

2020 ◽  
Vol 45 (5) ◽  
Author(s):  
A. Bamisaye ◽  
C. O. Eromosele ◽  
E. O. Dare ◽  
O. A. Akinloye
Keyword(s):  
Oral Delivery ◽  
Castor Oil ◽  
Wistar Rats ◽  
Blood Chemistry ◽  
Diffusion Method ◽  
Hematological Indices ◽  
Control Set ◽  
Set Up ◽  
And Control ◽  
Histological Architecture

The emergence of nanocarrier for drug delivery has been flanked with great achievements in the field of nanomedicine. It is however unsafe to assume their toxicological safety. This necessitated examining the toxico-dynamics of ciprofloxacin loaded castor oil based emulsion (COAB+Cp). The emulsions were prepared by mechano-chemical process. The effects of 10 mL per kg of body-weight (BW) of COAB+Cp was estimated in Adult Wistar rats (n=10) for 21 days vis-à-vis a control set up, treated with equal volume of distilled water. The biochemical and haematological parameters were conducted with histopathology of the heart, kidney, spleen and liver. Also, the antimicrobial study on Bacillus. Subtilis (BS) was carried out using the agar well diffusion method. No observable abnormal change in BW gain of both test and control animals. Treatment of animals with COAB+Cp did not cause any observable significant change at P > 0.05 in the blood-chemistry parameters and hematological indices. The photopathological examinations indicates that the histological architecture of vital organs (heart, liver, kidney and spleen) was not compromised in COAB+Cp treated animals. While the recorded value of minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of COAB+Cp corroborated its mean zone of inhibition (ZI) values, signifying a better bioactivity of COAB+Cp compared to Cp on BS. COAB+Cp has no cardiotoxic, hematotoxic and hepatotoxic effects on Wistar rats, with increased potency of the encapsulated Cp thus appears promising as a safe vehicle for oral delivery of ciprofloxacin.

scholarly journals Antimicrobial Activity of Ibuprofen against Cystic Fibrosis-Associated Gram-Negative Pathogens

2018 ◽  
Vol 62 (3) ◽  
Author(s):  
Parth N. Shah ◽  
Kimberly R. Marshall-Batty ◽  
Justin A. Smolen ◽  
Jasur A. Tagaev ◽  
Qingquan Chen ◽  
...  
Keyword(s):  
Cystic Fibrosis ◽  
Antimicrobial Activity ◽  
Oral Delivery ◽  
Polymeric Nanoparticles ◽  
Pharmacokinetic Profile ◽  
Lavage Fluid ◽  
Therapeutic Effects ◽  
Content Type ◽  
Biodistribution Studies

ABSTRACTClinical trials have demonstrated the benefits of ibuprofen therapy in cystic fibrosis (CF) patients, an effect that is currently attributed to ibuprofen's anti-inflammatory properties. Yet, a few previous reports demonstrated an antimicrobial activity of ibuprofen as well, although none investigated its direct effects on the pathogens found in the CF lung, which is the focus of this work. Determination of ibuprofen'sin vitroantimicrobial activity againstPseudomonas aeruginosaandBurkholderiaspecies strains through measurements of the endpoint number of CFU and growth kinetics showed that ibuprofen reduced the growth rate and bacterial burden of the tested strains in a dose-dependent fashion. In anin vitroPseudomonasbiofilm model, a reduction in the rate of biomass accumulation over 8 h of growth with ibuprofen treatment was observed. Next, an acutePseudomonaspneumonia model was used to test this antimicrobial activity after the oral delivery of ibuprofen. Following intranasal inoculation, ibuprofen-treated mice exhibited lower CFU counts and improved survival compared with the control animals. Preliminary biodistribution studies performed after the delivery of ibuprofen to mice by aerosol demonstrated a rapid accumulation of ibuprofen in serum and minimum retention in lung tissue and bronchoalveolar lavage fluid. Therefore, ibuprofen-encapsulated polymeric nanoparticles (Ibu-NPs) were formulated to improve the pharmacokinetic profile. Ibu-NPs formulated for aerosol delivery inhibited the growth ofP. aeruginosa in vitroand may provide a convenient dosing method. These results provide an additional explanation for the previously observed therapeutic effects of ibuprofen in CF patients and further strengthen the argument for its use by these patients.

scholarly journals Oral Delivery of Curcumin Polymeric Nanoparticles Ameliorates CCl4-Induced Subacute Hepatotoxicity in Wistar Rats

Polymers ◽  
2018 ◽  
Vol 10 (5) ◽  
pp. 541 ◽  
Author(s):  
Gregory Marslin ◽  
Jose Prakash ◽  
Shanshan Qi ◽  
Gregory Franklin
Keyword(s):  
Oral Delivery ◽  
Wistar Rats ◽  
Polymeric Nanoparticles

New and sensitive HPLC-UV method for concomitant quantification of a combination of antifilariasis drugs in rat plasma and organs after simultaneous oral administration

2021 ◽  
Vol 13 (7) ◽  
pp. 933-945
Author(s):  
Andi Dian Permana ◽  
Elly Wahyudin ◽  
Ismail ◽  
Muh. Nur Amir ◽  
Muh. Raihan ◽  
...  
Keyword(s):  
Oral Administration ◽  
Lymphatic Filariasis ◽  
Wistar Rats ◽  
Rat Plasma ◽  
Simultaneous Analysis ◽  
Biodistribution Studies

A sensitive HPLC-UV method for simultaneous analysis of the drugs used in lymphatic filariasis treatment, namely IVM, ABZ and DOX has been validated. The method was applied in pharmacokinetic and biodistribution studies in Wistar rats.

Formulation, Optimization and in vitro / in vivo characterization of Spray Dried Doxorubicin Loaded Folic Acid Conjugated Gelatin Nanoparticles

2020 ◽  
Vol 13 ◽  
Author(s):  
Harjit Singh ◽  
Bharat Khurana ◽  
Daisy Arora ◽  
Sukhbir Kaur
Keyword(s):  
Folic Acid ◽  
Cell Viability ◽  
Wistar Rats ◽  
Entrapment Efficiency ◽  
Gelatin Nanoparticles ◽  
Formulation Optimization ◽  
Biodistribution Studies ◽  
Spray Dried

Purpose: Formulation, optimization and anticancer activity of spray-dried Doxorubicin loaded folic acid conjugated Gelatin nanoparticles (DOX-FA-GN). Method: Doxorubicin loaded gelatin nanoparticles (DOX-GN) were prepared by the Coacervation phase separation method, optimized using DoE and then conjugated with folic acid by covalent coupling to formulate Doxorubicin loaded folic acid conjugated nanoparticles (DOX-FA-GN). The formulated nanoparticles were characterized to evaluate its physicochemical properties. Cellular uptake and cell viability studies were carried out using MTT assay and biodistribution studies were carried out in Wistar rats. Result: Particle size, PDI and entrapment efficiency for optimized DOX-GN was found to be 152.3 ± 9.3 nm 0.294 ± 0.1 and 86.9± 3.4 % while for DOX-FA-GN, 193.9 ± 12.3 nm 0.247 ± 0.2 and 84 ± 3.6 %. The cytotoxic studies showed a cell viability of 75.1% for DOX-GN and 29.5 % DOX-FA-GN. Biodistribution studies found to be statistically insignificant for conjugated nanoparticles with excellent flow properties. Significantly higher DOX distribution in the lungs was observed in the case of DOX-FA-GN. Conclusions: There was a higher uptake of DOX on HeLa cells with DOX-FA-GN compared to DOX-GN. Also, the biodistribution of Dox in the lungs of Wistar rats was higher in conjugated nanoparticles as compared to unconjugated nanoparticles.

Enhanced Pharmacokinetic Activity of Zotepine via Nanostructured Lipid Carrier System in Wistar Rats for Oral Application

2020 ◽  
Vol 8 (2) ◽  
pp. 148-160 ◽  
Author(s):  
Cernam Tirumalesh ◽  
Dinesh Suram ◽  
Narendar Dudhipala ◽  
Nagaraj Banala
Keyword(s):  
Oral Bioavailability ◽  
Oral Delivery ◽  
Wistar Rats ◽  
Water Solubility ◽  
In Vitro Release ◽  
Log P ◽  
Nanostructured Lipid Carrier ◽  
Vitro Release ◽  
Sem Studies

Background: Zotepine (ZT) is a substituted dibenzothiepine tricyclic molecule and second generation antipsychotic drug. It is available as the parenteral and oral solid dosage form, but, orally administered ZT has a poor oral bioavailability (10%) that might be due to either poor water solubility, high lipophilicity (Log P 4) and also first-pass hepatic metabolism. Objective: The oral bioavailability of ZT was improved by loading into a nanostructured lipid carriers (NLCs) system. Methods: Hot homogenization with probe sonication method was used for the preparation of ZT-NLCs formulations and characterized for an optimal system based on physicochemical characteristics and in vitro release. Differential scanning calorimetry (DSC), X-ray diffraction (XRD) analysis, and scanning electron microscopy (SEM) studies were used to confirm the crystalline nature and shape of the optimized ZT-NLC formulation. The physical stability of the optimized ZT-NLC formulation was evaluated at the refrigerator and room temperature over two months. Furthermore, in vivo pharmacokinetic (PK) studies of optimized ZT-NLC and ZT coarse suspension (ZT-CS) as control formulation, were conducted in male Wistar rats. Results: The optimized formulation of ZT-NLC showed Z-avg, PDI, ZP of 145.8 ± 2.5 nm, 0.18 ± 0.05, -31.6 ± 1.8 mV, respectively. In vitro release studies indicated the sustained release of ZT. DSC and XRD studies revealed the conversion of ZT into an amorphous form. SEM studies showed the spherical shape of the ZT-NLC formulation. PK studies showed 1.8-folds improvement (p<0.05) in oral bioavailability when compared with ZTCS formulation. Conclusion: Overall, the results established that NLCs could be used as a new alternative delivery vehicle for the oral delivery of ZT.

Radiolabelled 153Sm-chelates of glycoconjugates: multivalence and topology effects on the targeting of the asialoglycoprotein receptor

2007 ◽  
Vol 95 (6) ◽  
Author(s):  
Susana Torres ◽  
Jose A. Martins ◽  
João P. André ◽  
Maria Neves ◽  
Ana C. Santos ◽  
...  
Keyword(s):  
Wistar Rats ◽  
Asialoglycoprotein Receptor ◽  
And Topology ◽  
Biodistribution Studies

In this paper we report and discuss the biodistribution studies with Wistar rats of a series of

scholarly journals In Vitro and In Vivo Behaviour of I111n Complexes of TTHA, TTHA-Bis(Butylamide) and TTHA-Bis(Glucamide): Stability, Biodistribution and Excretion Studied by Gamma Imaging

1998 ◽  
Vol 5 (5) ◽  
pp. 259-264
Author(s):  
M. I. M. Prata ◽  
M. J. Ribeiro ◽  
A. C. Santos ◽  
J. A. Peters ◽  
F. Nepveu ◽  
...  
Keyword(s):  
Wistar Rats ◽  
Renal Excretion ◽  
Gamma Imaging ◽  
In Vivo Biodistribution ◽  
Biodistribution Studies ◽  
In Vitro Stability

Aiming at radiopharmaceutical application, I111n3+ complexes of the polyaminocarboxylates TTHA, TTHA-bis(butylamide) and TTHA-bis(glucamide) were investigated. The in vitro stability of I111n(TTHA)3− and I111n(TTHA-bis(butylamide)- was evaluated by measuring the exchange of I111n3+ from the complexes to transferrin and the results were compared with those for I111n(DTPA)2−. We also performed biodistribution studies of the three I111n3+ complexes by gamma-imaging in Wistar rats and by measuring the radioactivity in their organs. TTHA and its derivatives seem to have similar in vivo biodistribution with prevailing renal excretion.

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