Repetitive exposure to zinc oxide nanoparticles induces dna damage in human nasal mucosa mini organ cultures

2011 ◽  
Vol 52 (7) ◽  
pp. 582-589 ◽  
Author(s):  
Stephan Hackenberg ◽  
Franz-Zeno Zimmermann ◽  
Agmal Scherzed ◽  
Gudrun Friehs ◽  
Katrin Froelich ◽  
...  
Keyword(s):  
Dna Damage ◽  
Zinc Oxide ◽  
Nasal Mucosa ◽  
Zinc Oxide Nanoparticles ◽  
Oxide Nanoparticles ◽  
Organ Cultures ◽  
Human Nasal Mucosa ◽  
Repetitive Exposure

Cytotoxic, genotoxic and pro-inflammatory effects of zinc oxide nanoparticles in human nasal mucosa cells in vitro

2011 ◽  
Vol 25 (3) ◽  
pp. 657-663 ◽  
Author(s):  
Stephan Hackenberg ◽  
Agmal Scherzed ◽  
Antje Technau ◽  
Michael Kessler ◽  
Katrin Froelich ◽  
...  
Keyword(s):  
Zinc Oxide ◽  
Nasal Mucosa ◽  
Zinc Oxide Nanoparticles ◽  
Oxide Nanoparticles ◽  
Human Nasal Mucosa

scholarly journals The Radiosensitizing Effect of Zinc Oxide Nanoparticles in Sub-Cytotoxic Dosing Is Associated with Oxidative Stress In Vitro

Materials ◽  
2019 ◽  
Vol 12 (24) ◽  
pp. 4062
Author(s):  
Till Jasper Meyer ◽  
Agmal Scherzad ◽  
Helena Moratin ◽  
Thomas Eckert Gehrke ◽  
Julian Killisperger ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Dna Damage ◽  
Zinc Oxide ◽  
Zinc Oxide Nanoparticles ◽  
Oxidative Dna Damage ◽  
Clonogenic Survival ◽  
Oxide Nanoparticles ◽  
Radiosensitizing Effect ◽  
Primary Fibroblasts

Radioresistance is an important cause of head and neck cancer therapy failure. Zinc oxide nanoparticles (ZnO-NP) mediate tumor-selective toxic effects. The aim of this study was to evaluate the potential for radiosensitization of ZnO-NP. The dose-dependent cytotoxicity of ZnO-NP20 nm and ZnO-NP100 nm was investigated in FaDu and primary fibroblasts (FB) by an MTT assay. The clonogenic survival assay was used to evaluate the effects of ZnO-NP alone and in combination with irradiation on FB and FaDu. A formamidopyrimidine-DNA glycosylase (FPG)-modified single-cell microgel electrophoresis (comet) assay was applied to detect oxidative DNA damage in FB as a function of ZnO-NP and irradiation exposure. A significantly increased cytotoxicity after FaDu exposure to ZnO-NP20 nm or ZnO-NP100 nm was observed in a concentration of 10 µg/mL or 1 µg/mL respectively in 30 µg/mL of ZnO-NP20 nm or 20 µg/mL of ZnO-NP100 nm in FB. The addition of 1, 5, or 10 µg/mL ZnO-NP20 nm or ZnO-NP100 nm significantly reduced the clonogenic survival of FaDu after irradiation. The sub-cytotoxic dosage of ZnO-NP100 nm increased the oxidative DNA damage compared to the irradiated control. This effect was not significant for ZnO-NP20 nm. ZnO-NP showed radiosensitizing properties in the sub-cytotoxic dosage. At least for the ZnO-NP100 nm, an increased level of oxidative stress is a possible mechanism of the radiosensitizing effect.

Assessment of oxidative/nitrosative stress biomarkers and DNA damage in Haemonchus contortus, following exposure to zinc oxide nanoparticles

2018 ◽  
Vol 63 (3) ◽  
pp. 563-571 ◽  
Author(s):  
Bijan Esmaeilnejad ◽  
Awat Samiei ◽  
Yousef Mirzaei ◽  
Farhad Farhang-Pajuh
Keyword(s):  
Dna Damage ◽  
Zinc Oxide ◽  
Zinc Oxide Nanoparticles ◽  
Metallic Nanoparticles ◽  
Nitrosative Stress ◽  
Total Antioxidant Status ◽  
Parasitic Infections ◽  
Oxide Nanoparticles ◽  
Helminth Parasites ◽  
Zno Nps

Abstract Drug resistance in helminth parasites has incurred several difficulties to livestock industry and ranked among the top public health concerns. Therefore, seeking for new agents to control parasites is an urgent strategy. In the recent years, metallic nanoparticles have been considerably evaluated for anthelmintic effects. The current research was conducted to assess possible anthelmintic impacts of zinc oxide nanoparticles (ZnO-NPs) on a prevalent gastrointestinal nematode, H. contortus. Moreover, several biomarkers of oxidative/nitrosative stress and DNA damage were measured. Various concentrations of the nanoparticle were prepared and incubated with the worms for 24 hours. The parasite mobility, mortality rate, antioxidant enzymes activities (SOD, Catalase and GSH-Px), lipid peroxidation, total antioxidant status as well as nitric oxide (NO) contents and DNA damage were determined. ZnO-NPs exerted significant wormicidal effects via induction of oxidative/nitrosative stress and DNA damage. Conclusively, ZnO-NPs can be utilized as a novel and potential agent to control and treatment of helminth parasitic infections.

Assessment of oxidative/nitrosative stress biomarkers and DNA damage in Teladorsagia circumcincta following exposure to zinc oxide nanoparticles

2020 ◽  
Vol 94 ◽  
Author(s):  
Z. Baghbani ◽  
B. Esmaeilnejad ◽  
S. Asri-Rezaei
Keyword(s):  
Dna Damage ◽  
Zinc Oxide ◽  
Zinc Oxide Nanoparticles ◽  
Nitrosative Stress ◽  
Gastrointestinal Nematodes ◽  
Parasitic Infections ◽  
Oxide Nanoparticles ◽  
Helminth Parasites ◽  
Zno Nps ◽  
Teladorsagia Circumcincta

Abstract Drug resistance to helminth parasites is one of the most serious problems to threaten the livestock industry. The problem also poses a major threat to public health. Therefore, novel and safe agents should urgently be investigated to control parasitic infections. The current study was conducted to evaluate the possible antiparasitic effects of zinc oxide nanoparticles (ZnO-NPs) on one of the most prevalent gastrointestinal nematodes, Teladorsagia circumcincta. The worms were incubated with various concentrations of ZnO-NPs: 1, 4, 8, 12 and 16 ppm for 24 hours. Mobility and mortality of the parasites were recorded at four-hour intervals. At the endpoint, several biomarkers of oxidative/nitrosative stress, including superoxide dismutase, glutathione peroxidase and catalase, as well as lipid peroxidation, protein carbonylation, total antioxidant status, nitric oxide contents and DNA damage, were measured in the homogenized samples. ZnO-NPs showed significant anthelminthic effects, depending on time and concentration. Furthermore, the nanoparticle induced severe oxidative/nitrosative stress and DNA damage. ZnO-NPs could be considered as a novel and potent anthelminthic agent.

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