scholarly journals Design and Fabrication of Dual Redox Responsive Nanoparticles with Diselenide Linkage Combined Photodynamically to Effectively Enhance Gene Expression

2020 ◽  
Vol Volume 15 ◽  
pp. 7297-7314
Author(s):  
Yan Fang ◽  
Xiaojie Lin ◽  
Xuechao Jin ◽  
Dongjuan Yang ◽  
Shan Gao ◽  
...  
Keyword(s):  
Gene Expression ◽  
Redox Responsive ◽  
Enhance Gene Expression

scholarly journals A modular toolset for electrogenetics

2021 ◽  
Author(s):  
Joshua M Lawrence ◽  
Yutong Yin ◽  
Paolo Bombelli ◽  
Alberto Scarampi ◽  
Marko Storch ◽  
...  
Keyword(s):  
Gene Expression ◽  
Industrial Applications ◽  
Electrochemical Activation ◽  
Genetic Circuits ◽  
Control Of Gene Expression ◽  
Promoter Library ◽  
Redox Responsive ◽  
Electrochemical Control ◽  
Redox Molecules ◽  
New Applications

Synthetic biology research and its industrial applications rely on the deterministic spatiotemporal control of gene expression. Recently, electrochemical control of gene expression has been demonstrated in electrogenetic systems (redox-responsive promoters used alongside redox inducers and an electrode), allowing for the direct integration of electronics with complex biological processes for a variety of new applications. However, the use of electrogenetic systems is limited by poor activity, tunability and standardisation. Here, we have developed a variety of genetic and electrochemical tools that facilitate the design and vastly improve the performance of electrogenetic systems. We developed a strong, unidirectional, redox-responsive promoter before deriving a mutant promoter library with a spectrum of strengths. We then constructed genetic circuits with these parts and demonstrated their activation by multiple classes of redox molecules. Finally, we demonstrated electrochemical activation of gene expression in aerobic conditions utilising a novel, modular bioelectrochemical device. This toolset provides researchers with all the elements needed to design and build optimised electrogenetic systems for specific applications.

scholarly journals Nanomaterials Enhanced Gene Expression in Yeast Cells

2008 ◽  
Vol 2008 ◽  
pp. 1-9 ◽  
Author(s):  
Su-Fang Chien ◽  
Shi-Hui Chen ◽  
Chhiu-Tsu Lin
Keyword(s):  
Gene Expression ◽  
Ag Nanoparticles ◽  
Product Yield ◽  
Protein Band ◽  
Yeast Cells ◽  
Vis Spectroscopy ◽  
Yeast Chromosome ◽  
Single Protein ◽  
Enhance Gene Expression ◽  
Metal Nanomaterials

Metal nanomaterials are shown to enhance gene expression for rice -galactosidase gene (-Gal) in yeast cells. Au and Ag nanoparticles and their nanocomposites, silica-Au and silica-Ag, were prepared and characterized by UV-vis spectroscopy and TEM technique. The rice -galactosidase gene was cloned into the yeast chromosome, where the cloned cells were precultured and induced into a medium containing each of the testing nanomaterials. The nanomaterials were observed to incorporate inside the cells, and no cell death has been detected during the course of gene expression. The enzyme activity was determined by a synthetic substrate, p-nitrophenyl--D-galctopyranoside, and the yellow product yield was recorded in a spectrophotometer at 400 nm. When Au and Ag nanoparticles were incorporated with the culture, a 3–5 fold enhancement in -galactosidase was observed for intracellular activity as well as the secreted activity into the medium. The secreted protein was analyzed to have a pure form and displayed as a single protein band in the SDS-gel electrophoresis. The effects of size and chemical nature of nanomaterials on gene expression for the rice -galactosidase gene in yeast cells are discussed.

Electrospun poly(l-lactide-co-caprolactone)–collagen–chitosan vascular graft in a canine femoral artery model

2015 ◽  
Vol 3 (28) ◽  
pp. 5760-5768 ◽  
Author(s):  
Tong Wu ◽  
Bojie Jiang ◽  
Yuanfei Wang ◽  
Anlin Yin ◽  
Chen Huang ◽  
...  
Keyword(s):  
Gene Expression ◽  
Tissue Regeneration ◽  
Femoral Artery ◽  
Vascular Graft ◽  
Patency Rate ◽  
Vascular Grafts ◽  
Enhance Gene Expression

(P(LLA-CL)–COL–CS) composite vascular grafts could effectively improve patency rate, promote tissue regeneration, and enhance gene expression.

The application of powerful promoters to enhance gene expression in industrial microorganisms

2017 ◽  
Vol 33 (2) ◽  
Author(s):  
Shenghu Zhou ◽  
Guocheng Du ◽  
Zhen Kang ◽  
Jianghua Li ◽  
Jian Chen ◽  
...  
Keyword(s):  
Gene Expression ◽  
Enhance Gene Expression

Circulating γ/δ T cells in systemic sclerosis exhibit activated phenotype and enhance gene expression of proalpha2(I) collagen of fibroblasts

2013 ◽  
Vol 69 (1) ◽  
pp. 54-60 ◽  
Author(s):  
Ikuko Ueda-Hayakawa ◽  
Minoru Hasegawa ◽  
Yasuhito Hamaguchi ◽  
Kazuhiko Takehara ◽  
Manabu Fujimoto
Keyword(s):  
Gene Expression ◽  
T Cells ◽  
Systemic Sclerosis ◽  
Enhance Gene Expression
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