Electrospun poly(l-lactide-co-caprolactone)–collagen–chitosan vascular graft in a canine femoral artery model

2015 ◽  
Vol 3 (28) ◽  
pp. 5760-5768 ◽  
Author(s):  
Tong Wu ◽  
Bojie Jiang ◽  
Yuanfei Wang ◽  
Anlin Yin ◽  
Chen Huang ◽  
...  
Keyword(s):  
Gene Expression ◽  
Tissue Regeneration ◽  
Femoral Artery ◽  
Vascular Graft ◽  
Patency Rate ◽  
Vascular Grafts ◽  
Enhance Gene Expression

(P(LLA-CL)–COL–CS) composite vascular grafts could effectively improve patency rate, promote tissue regeneration, and enhance gene expression.

scholarly journals Engineering the Composition of Microfibers to Enhance the Remodeling of a Cell-Free Vascular Graft

Nanomaterials ◽  
2021 ◽  
Vol 11 (6) ◽  
pp. 1613
Author(s):  
Fang Huang ◽  
Yu-Fang Hsieh ◽  
Xuefeng Qiu ◽  
Shyam Patel ◽  
Song Li
Keyword(s):  
Vascular Graft ◽  
Animal Studies ◽  
Vascular Grafts ◽  
Cell Infiltration ◽  
Tubular Scaffold ◽  
Base Polymer ◽  
A Cell ◽  
Scaffold Materials ◽  
Stromal Cell Derived Factor ◽  
Technology Blending

The remodeling of vascular grafts is critical for blood vessel regeneration. However, most scaffold materials have limited cell infiltration. In this study, we designed and fabricated a scaffold that incorporates a fast-degrading polymer polydioxanone (PDO) into the microfibrous structure by means of electrospinning technology. Blending PDO with base polymer decreases the density of electrospun microfibers yet did not compromise the mechanical and structural properties of the scaffold, and effectively enhanced cell infiltration. We then used this technique to fabricate a tubular scaffold with heparin conjugated to the surface to suppress thrombosis, and the construct was implanted into the carotid artery as a vascular graft in animal studies. This graft significantly promoted cell infiltration, and the biochemical cues such as immobilized stromal cell-derived factor-1α further enhanced cell recruitment and the long-term patency of the grafts. This work provides an approach to optimize the microfeatures of vascular grafts, and will have broad applications in scaffold design and fabrication for regenerative engineering.

Temporal changes in regeneration-associated gene expression in cryopreserved rat vascular grafts

2003 ◽  
Vol 35 (1) ◽  
pp. 506-508
Author(s):  
T Kiji ◽  
Y Dohi ◽  
K Nishizaki ◽  
H Sakaguchi ◽  
S Nagasaka ◽  
...  
Keyword(s):  
Gene Expression ◽  
Vascular Grafts ◽  
Temporal Changes

Analyzing Gene Expression through Real Time PCR while Neo-tissue Regeneration using Developed Tissue Constructs

2020 ◽  
pp. 15-34
Keyword(s):  
Gene Expression ◽  
Real Time ◽  
Tissue Regeneration ◽  
Real Time Pcr ◽  
Molecular Level ◽  
Northern Blotting ◽  
Tissue Constructs ◽  
Low Sensitivity

Real-time PCR offers a wide area of application to analyze the role of gene activity in various biological aspects at the molecular level with higher specificity, sensitivity and the potential to troubleshoot with post-PCR processing and difficulties. With the recent advancement in the development of functional tissue graft for the regeneration of damaged/diseased tissue, it is effective to analyze the cell behaviour and differentiation over tissue construct toward specific lineage through analyzing the expression of an array of specific genes. With the ability to collect data in the exponential phase, the application of Real-Time PCR has been expanded into various fields such as tissue engineering ranging from absolute quantification of gene expression to determine neo-tissue regeneration and its maturation. In addition to its usage as a research tool, numerous advancements in molecular diagnostics have been achieved, including microbial quantification, determination of gene dose and cancer research. Also, in order to consistently quantify mRNA levels, Northern blotting and in situ hybridization (ISH) methods are less preferred due to low sensitivity, poor precision in detecting gene expression at a low level. An amplification step is thus frequently required to quantify mRNA amounts from engineered tissues of limited size. When analyzing tissue-engineered constructs or studying biomaterials–cells interactions, it is pertinent to quantify the performance of such constructs in terms of extracellular matrix formation while in vitro and in vivo examination, provide clues regarding the performance of various tissue constructs at the molecular level. In this chapter, our focus is on Basics of qPCR, an overview of technical aspects of Real-time PCR; recent Protocol used in the lab, primer designing, detection methods and troubleshooting of the experimental problems.

scholarly journals Cell-Free Demineralized Bone Matrix for Mesenchymal Stem Cells Survival and Colonization

Materials ◽  
2019 ◽  
Vol 12 (9) ◽  
pp. 1360 ◽  
Author(s):  
Monica Mattioli-Belmonte ◽  
Francesca Montemurro ◽  
Caterina Licini ◽  
Iolanda Iezzi ◽  
Manuela Dicarlo ◽  
...  
Keyword(s):  
Gene Expression ◽  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Tissue Regeneration ◽  
Demineralized Bone Matrix ◽  
Bone Matrix ◽  
Morphological Observation ◽  
Type I ◽  
Demineralized Bone

Decellularized bone matrix is receiving much attention as biological scaffolds and implantable biomaterials for bone tissue regeneration. Here, we evaluated the efficacy of a cell-free demineralized bone matrix on mesenchymal stem cells (MSCs) survival and differentiation in vitro. The seeding of human umbilical cord-derived MSCs (hUC-SCs) on decellularized bone matrices up to 14 days was exploited, assessing their capability of scaffold colonization and evaluating gene expression of bone markers. Light and Scanning Electron Microscopies were used. The obtained cell-free decalcified structures showed elastic moduli attributable to both topology and biochemical composition. Morphological observation evidenced an almost complete colonization of the scaffolds after 14 days of culture. Moreover, in hUC-SCs cultured on decalcified scaffolds, without the addition of any osteoinductive media, there was an upregulation of Collagen Type I (COL1) and osteonectin (ON) gene expression, especially on day 14. Modifications in the expression of genes engaged in stemness were also detected. In conclusion, the proposed decellularized bone matrix can induce the in vitro hUC-SCs differentiation and has the potential to be tested for in in vivo tissue regeneration.

PLATELET DEPOSITION AND PSEUDO-INTIMAL HYPERPLASIA IN PROSTHETIC VASCULAR GRAFTS

1987 ◽  
Author(s):  
R A Harper ◽  
I F Lane ◽  
C M Backhouse ◽  
C N McCollum ◽  
A C Meek
Keyword(s):  
Femoral Artery ◽  
Intimal Hyperplasia ◽  
Vascular Grafts ◽  
Graft Occlusion ◽  
Intimal Thickening ◽  
Arterial Grafts ◽  
Post Operative Period ◽  
Platelet Deposition ◽  
Platelet Accumulation ◽  
The Relationship

Platelet and fibrin accumulation in arterial grafts may cause pseudo-intimal hyperplasia and graft occlusion. The relationship between the rate of post-operative platelet accumulation and subsequent pseudo-intimal hyperplasia has been studied in prosthetic grafts implanted in greyhounds.The femoral artery in 30 greyhounds was replaced by a 6cm length of 6mm PTFE. Autologous 111In-platelet deposition over the graft was measured by probe and ratemeter for 7 days and radioactivity compared to the contralateral thigh. The daily increase in this ratio graft over reference was calculated as the Thrombogenicity Index (TI). Grafts were removed at 8 weeks and sectioned at 5, 30 and 55mm for measurement of pseudo-intimal thickening by grid microscopy.The animals subsequently developing occlusion or pseudo-intimal harrowing of greater than 50% of the lumen had a markedly greater TI of 0.22±0.027 compared to 0.03±0.019 in the 23 grafts maintaining wide patency (p<0.05). TI was highest in the 4 grafts which occluded at 0.3110.09 compared to 0.04±0.02 in the 26 that remained patent (p<0.01). There was a highly significant correlation (r=0.69) between post-operative TI and subsequent pseudo-intimal hyperplasia (p<0.001).Platelet deposition in the early post-operative period appears to promote the development of progressive pseudo-intimal thickening and ultimate occlusion

scholarly journals Decoding an organ regeneration switch by dissecting cardiac regeneration enhancers

Development ◽  
2020 ◽  
Vol 147 (24) ◽  
pp. dev194019
Author(s):  
Ian J. Begeman ◽  
Kwangdeok Shin ◽  
Daniel Osorio-Méndez ◽  
Andrew Kurth ◽  
Nutishia Lee ◽  
...  
Keyword(s):  
Gene Expression ◽  
Tissue Regeneration ◽  
Cardiac Injury ◽  
Transcriptional Response ◽  
Cardiac Regeneration ◽  
Regulatory Elements ◽  
Heart Regeneration ◽  
New Paradigm ◽  
Closely Related Species ◽  
Organ Regeneration

ABSTRACTHeart regeneration in regeneration-competent organisms can be accomplished through the remodeling of gene expression in response to cardiac injury. This dynamic transcriptional response relies on the activities of tissue regeneration enhancer elements (TREEs); however, the mechanisms underlying TREEs are poorly understood. We dissected a cardiac regeneration enhancer in zebrafish to elucidate the mechanisms governing spatiotemporal gene expression during heart regeneration. Cardiac lepb regeneration enhancer (cLEN) exhibits dynamic, regeneration-dependent activity in the heart. We found that multiple injury-activated regulatory elements are distributed throughout the enhancer region. This analysis also revealed that cardiac regeneration enhancers are not only activated by injury, but surprisingly, they are also actively repressed in the absence of injury. Our data identified a short (22 bp) DNA element containing a key repressive element. Comparative analysis across Danio species indicated that the repressive element is conserved in closely related species. The repression mechanism is not operational during embryogenesis and emerges when the heart begins to mature. Incorporating both activation and repression components into the mechanism of tissue regeneration constitutes a new paradigm that might be extrapolated to other regeneration scenarios.

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