scholarly journals Novel magnetic fibrin hydrogel scaffolds containing thrombin and growth factors conjugated iron oxide nanoparticles for tissue engineering

2012 ◽  
pp. 1259 ◽  
Author(s):  
Ofra Ziv-Polat ◽  
Skaat ◽  
Shahar ◽  
Shlomo Margel
Keyword(s):  
Tissue Engineering ◽  
Growth Factors ◽  
Iron Oxide ◽  
Iron Oxide Nanoparticles ◽  
Oxide Nanoparticles ◽  
Hydrogel Scaffolds

A Hybrid Nano-Bioprinting Device for Tissue Engineering

2010 ◽  
Author(s):  
Kivilcim Buyukhatipoglu ◽  
Wei Sun ◽  
Alisa Morss Clyne
Keyword(s):  
Tissue Engineering ◽  
Iron Oxide ◽  
Iron Oxide Nanoparticles ◽  
Tissue Growth ◽  
Superparamagnetic Nanoparticles ◽  
Oxide Nanoparticles ◽  
3D Architecture ◽  
Tissue Engineering Construct ◽  
Direct Cell

Tissue engineering may require precise patterning of cells and bioactive factors to recreate the complex, 3D architecture of native tissue. These cells and bioactive components may then need to be repositioned during tissue growth in vitro and noninvasively imaged to track tissue development. We developed a new hybrid nano-bioprinting system by combining the initial patterning capabilities of a direct cell writing system with the active patterning capabilities of superparamagnetic nanoparticles. The iron oxide nanoparticles can be conjugated with proteins or loaded inside cells, printed into computer-defined patterns, and then manipulated and imaged within the 3D tissue engineering construct. In this study, iron oxide nanoparticles were bioprinted either in an alginate scaffold or inside endothelial cells. Cell viability, patterning, and imaging were assessed.

scholarly journals Iron Oxide Nanoparticles in Regenerative Medicine and Tissue Engineering

Nanomaterials ◽  
2021 ◽  
Vol 11 (9) ◽  
pp. 2337
Author(s):  
Ralf P. Friedrich ◽  
Iwona Cicha ◽  
Christoph Alexiou
Keyword(s):  
Tissue Engineering ◽  
Iron Oxide ◽  
Regenerative Medicine ◽  
Iron Oxide Nanoparticles ◽  
Oxide Nanoparticles ◽  
Specific Cell ◽  
Non Invasive ◽  
Observation Methods ◽  
Therapeutic Compounds ◽  
Kidney Liver

In recent years, many promising nanotechnological approaches to biomedical research have been developed in order to increase implementation of regenerative medicine and tissue engineering in clinical practice. In the meantime, the use of nanomaterials for the regeneration of diseased or injured tissues is considered advantageous in most areas of medicine. In particular, for the treatment of cardiovascular, osteochondral and neurological defects, but also for the recovery of functions of other organs such as kidney, liver, pancreas, bladder, urethra and for wound healing, nanomaterials are increasingly being developed that serve as scaffolds, mimic the extracellular matrix and promote adhesion or differentiation of cells. This review focuses on the latest developments in regenerative medicine, in which iron oxide nanoparticles (IONPs) play a crucial role for tissue engineering and cell therapy. IONPs are not only enabling the use of non-invasive observation methods to monitor the therapy, but can also accelerate and enhance regeneration, either thanks to their inherent magnetic properties or by functionalization with bioactive or therapeutic compounds, such as drugs, enzymes and growth factors. In addition, the presence of magnetic fields can direct IONP-labeled cells specifically to the site of action or induce cell differentiation into a specific cell type through mechanotransduction.

scholarly journals Magnetic Targeting of Growth Factors Using Iron Oxide Nanoparticles

Nanomaterials ◽  
2018 ◽  
Vol 8 (9) ◽  
pp. 707 ◽  
Author(s):  
Michal Marcus ◽  
Alexandra Smith ◽  
Ahmad Maswadeh ◽  
Ziv Shemesh ◽  
Idan Zak ◽  
...  
Keyword(s):  
Growth Factors ◽  
Iron Oxide ◽  
Magnetic Fields ◽  
Iron Oxide Nanoparticles ◽  
Drug Carriers ◽  
Model Systems ◽  
Mouse Retina ◽  
Oxide Nanoparticles ◽  
Magnetic Targeting ◽  
Magnetic Carriers

Growth factors play an important role in nerve regeneration and repair. An attractive drug delivery strategy, termed “magnetic targeting”, aims to enhance therapeutic efficiency by directing magnetic drug carriers specifically to selected cell populations that are suitable for the nervous tissues. Here, we covalently conjugated nerve growth factor to iron oxide nanoparticles (NGF-MNPs) and used controlled magnetic fields to deliver the NGF–MNP complexes to target sites. In order to actuate the magnetic fields a modular magnetic device was designed and fabricated. PC12 cells that were plated homogenously in culture were differentiated selectively only in targeted sites out of the entire dish, restricted to areas above the magnetic “hot spots”. To examine the ability to guide the NGF-MNPs towards specific targets in vivo, we examined two model systems. First, we injected and directed magnetic carriers within the sciatic nerve. Second, we injected the MNPs intravenously and showed a significant accumulation of MNPs in mouse retina while using an external magnet that was placed next to one of the eyes. We propose a novel approach to deliver drugs selectively to injured sites, thus, to promote an effective repair with minimal systemic side effects, overcoming current challenges in regenerative therapeutics.

scholarly journals Interaction between Iron Oxide Nanoparticles and HepaRG Cells: A PreliminaryIn VitroEvaluation

2015 ◽  
Vol 2015 ◽  
pp. 1-9 ◽  
Author(s):  
M. Helvenstein ◽  
D. Stanicki ◽  
S. Laurent ◽  
B. Blankert
Keyword(s):  
Magnetic Resonance Imaging ◽  
Magnetic Field ◽  
Tissue Engineering ◽  
Iron Oxide ◽  
Iron Oxide Nanoparticles ◽  
Oxide Nanoparticles ◽  
Resonance Imaging ◽  
Heparg Cells ◽  
Magnetic Labeling ◽  
Metabolite Generation

Nowadays, the use of iron oxide nanoparticles is widespread to label cells for magnetic resonance imaging tracking. More recently, magnetic labeling provides promising new opportunities for tissue engineering by controlling and manipulating cells through the action of an external magnetic field. The present work describes nonspecific labeling of metabolically competent HepaRG hepatocytes with anionic iron oxide nanoparticles. An interaction was observed between nanoparticles and studied cells, which were easily attracted when exposed to a magnet. No cytotoxicity was detected in the hepatocytes after 24 hours of incubation with iron oxide nanoparticles. Impact on HepaRG metabolization activity was assessed. Although a slight decrease in the metabolite generation was observed after exposure to nanoparticles (2 mM in iron), the enzymatic capacity was maintained. These results pave the way for 3D cultures of magnetic labeled HepaRG cells by using a magnetic field.

Magnetic Tissue Engineering of the Vocal Fold Using Superparamagnetic Iron Oxide Nanoparticles

2019 ◽  
Vol 25 (21-22) ◽  
pp. 1470-1477 ◽  
Author(s):  
Marina Pöttler ◽  
Anna Fliedner ◽  
Julia Bergmann ◽  
Linh Katrin Bui ◽  
Marina Mühlberger ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Iron Oxide ◽  
Iron Oxide Nanoparticles ◽  
Vocal Fold ◽  
Superparamagnetic Iron Oxide ◽  
Superparamagnetic Iron Oxide Nanoparticles ◽  
Oxide Nanoparticles

TARGETING CYCLIN B1 WITH ANTISENSE OLIGONUCLETOTIDES- COATED SUPERPARAMAGNETIC IRON OXIDE NANOPARTICLES

2018 ◽  
Vol 6 (10) ◽  
Author(s):  
Hosam Zaghloul ◽  
Doaa A. Shahin ◽  
Ibrahim El- Dosoky ◽  
Mahmoud E. El-awady ◽  
Fardous F. El-Senduny ◽  
...  
Keyword(s):  
Iron Oxide ◽  
Iron Oxide Nanoparticles ◽  
Cellular Uptake ◽  
Superparamagnetic Iron Oxide ◽  
Cyclin B1 ◽  
Superparamagnetic Iron Oxide Nanoparticles ◽  
Oxide Nanoparticles ◽  
Mcf7 Cells ◽  
M Phase ◽  
The Impact

Antisense oligonucleotides (ASO) represent an attractive trend as specific targeting molecules but sustain poor cellular uptake meanwhile superparamagnetic iron oxide nanoparticles (SPIONs) offer stability of ASO and improved cellular uptake. In the present work we aimed to functionalize SPIONs with ASO targeting the mRNA of Cyclin B1 which represents a potential cancer target and to explore its anticancer activity. For that purpose, four different SPIONs-ASO conjugates, S-M (1–4), were designated depending on the sequence of ASO and constructed by crosslinking carboxylated SPIONs to amino labeled ASO. The impact of S-M (1–4) on the level of Cyclin B1, cell cycle, ROS and viability of the cells were assessed by flowcytometry. The results showed that S-M3 and S-M4 reduced the level of Cyclin B1 by 35 and 36%, respectively. As a consequence to downregulation of Cyclin B1, MCF7 cells were shown to be arrested at G2/M phase (60.7%). S-M (1–4) led to the induction of ROS formation in comparison to the untreated control cells. Furthermore, S-M (1–4) resulted in an increase in dead cells compared to the untreated cells and SPIONs-treated cells. In conclusion, targeting Cyclin B1 with ASO-coated SPIONs may represent a specific biocompatible anticancer strategy.

Ferrozine Assay for Simple and Cheap Iron Analysis of Silica-Coated Iron Oxide Nanoparticles

2018 ◽  
Author(s):  
Hattie Ring ◽  
Zhe Gao ◽  
Nathan D. Klein ◽  
Michael Garwood ◽  
John C. Bischof ◽  
...  
Keyword(s):  
Iron Oxide ◽  
Iron Oxide Nanoparticles ◽  
Hydrofluoric Acid ◽  
Inductively Coupled Plasma ◽  
Silica Shell ◽  
Oxide Nanoparticles ◽  
Inductively Coupled ◽  
Digestion Process ◽  
Plasma Methods ◽  
Iron Analysis

The Ferrozinen assay is applied as an accurate and rapid method to quantify the iron content of iron oxide nanoparticles (IONPs) and can be used in biological matrices. The addition of ascorbic aqcid accelerates the digestion process and can penetrate an IONP core within a mesoporous and solid silica shell. This new digestion protocol avoids the need for hydrofluoric acid to digest the surrounding silica shell and provides and accessible alternative to inductively coupled plasma methods. With the updated digestion protocol, the quantitative range of the Ferrozine assay is 1 - 14 ppm. <br>

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