scholarly journals Copper Oxide Nanoparticles Induce Oxidative DNA Damage and Cell Death via Copper Ion-Mediated P38 MAPK Activation in Vascular Endothelial Cells

2020 ◽  
Vol Volume 15 ◽  
pp. 3291-3302 ◽  
Author(s):  
Hui He ◽  
Zhen Zou ◽  
Bin Wang ◽  
Ge Xu ◽  
Chengzhi Chen ◽  
...  
Keyword(s):  
Dna Damage ◽  
Endothelial Cells ◽  
Cell Death ◽  
Oxidative Dna Damage ◽  
Vascular Endothelial Cells ◽  
Copper Ion ◽  
Copper Oxide Nanoparticles ◽  
Oxide Nanoparticles ◽  
Vascular Endothelial ◽  
Mapk Activation

scholarly journals Induction of Inflammation in Vascular Endothelial Cells by Metal Oxide Nanoparticles: Effect of Particle Composition

2007 ◽  
Vol 115 (3) ◽  
pp. 403-409 ◽  
Author(s):  
Andrea Gojova ◽  
Bing Guo ◽  
Rama S. Kota ◽  
John C. Rutledge ◽  
Ian M. Kennedy ◽  
...  
Keyword(s):  
Endothelial Cells ◽  
Metal Oxide ◽  
Vascular Endothelial Cells ◽  
Metal Oxide Nanoparticles ◽  
Oxide Nanoparticles ◽  
Vascular Endothelial ◽  
Particle Composition

scholarly journals Confluence of Vascular Endothelial Cells Induces Cell Cycle Exit by Inhibiting p42/p44 Mitogen-Activated Protein Kinase Activity

1999 ◽  
Vol 19 (4) ◽  
pp. 2763-2772 ◽  
Author(s):  
Francesc Viñals ◽  
Jacques Pouysségur
Keyword(s):  
Cell Cycle ◽  
Endothelial Cells ◽  
Growth Factors ◽  
Vascular Endothelial Cells ◽  
Mitogen Activated Protein Kinase ◽  
Vascular Endothelial ◽  
Cell Cycle Exit ◽  
Mapk Activation ◽  
Mapk Activity ◽  
Mitogen Activated Protein

ABSTRACT Like other cellular models, endothelial cells in cultures stop growing when they reach confluence, even in the presence of growth factors. In this work, we have studied the effect of cellular contact on the activation of p42/p44 mitogen-activated protein kinase (MAPK) by growth factors in mouse vascular endothelial cells. p42/p44 MAPK activation by fetal calf serum or fibroblast growth factor was restrained in confluent cells in comparison with the activity found in sparse cells. Consequently, the induction of c-fos, MAPK phosphatases 1 and 2 (MKP1/2), and cyclin D1 was also restrained in confluent cells. In contrast, the activation of Ras and MEK-1, two upstream activators of the p42/p44 MAPK cascade, was not impaired when cells attained confluence. Sodium orthovanadate, but not okadaic acid, restored p42/p44 MAPK activity in confluent cells. Moreover, lysates from confluent 1G11 cells more effectively inactivated a dually phosphorylated active p42 MAPK than lysates from sparse cells. These results, together with the fact that vanadate-sensitive phosphatase activity was higher in confluent cells, suggest that phosphatases play a role in the down-regulation of p42/p44 MAPK activity. Enforced long-term activation of p42/p44 MAPK by expression of the chimera ΔRaf-1:ER, which activates the p42/p44 MAPK cascade at the level of Raf, enhanced the expression of MKP1/2 and cyclin D1 and, more importantly, restored the reentry of confluent cells into the cell cycle. Therefore, inhibition of p42/p44 MAPK activation by cell-cell contact is a critical step initiating cell cycle exit in vascular endothelial cells.

Induction of Apoptotic Cell Death in Vascular Endothelial Cells Cultured in Three-Dimensional Collagen Lattice

1999 ◽  
Vol 248 (2) ◽  
pp. 498-508 ◽  
Author(s):  
Masafumi Kuzuya ◽  
Shosuke Satake ◽  
Miguel A. Ramos ◽  
Shigeru Kanda ◽  
Teruhiko Koike ◽  
...  
Keyword(s):  
Endothelial Cells ◽  
Cell Death ◽  
Vascular Endothelial Cells ◽  
Apoptotic Cell ◽  
Three Dimensional ◽  
Apoptotic Cell Death ◽  
Vascular Endothelial ◽  
Collagen Lattice ◽  
Cells Cultured

INTRACELLULAR SODIUM PROTECTS VASCULAR ENDOTHELIAL CELLS FROM APOPTOSIS TRIGGERED BY [3H]-DECAY-INDUCED DNA DAMAGE

2004 ◽  
Vol 22 (Suppl. 2) ◽  
pp. S50-S51
Author(s):  
S. Taurin ◽  
D. Pchejetski ◽  
N. Thorin-Trescase ◽  
J. Tremblay ◽  
P. Harriet ◽  
...  
Keyword(s):  
Dna Damage ◽  
Endothelial Cells ◽  
Vascular Endothelial Cells ◽  
Intracellular Sodium ◽  
Vascular Endothelial
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