scholarly journals Liquiritigenin-Loaded Submicron Emulsion Protects Against Doxorubicin-Induced Cardiotoxicity via Antioxidant, Anti-Inflammatory, and Anti-Apoptotic Activity

2020 ◽  
Vol Volume 15 ◽  
pp. 1101-1115 ◽  
Author(s):  
Changcan Shi ◽  
Hongjuan Wu ◽  
Ke Xu ◽  
Ting Cai ◽  
Kunming Qin ◽  
...  
Keyword(s):  
Anti Inflammatory ◽  
Apoptotic Activity ◽  
Submicron Emulsion

scholarly journals Ginsenoside Rk1 bioactivity: A systematic review

2017 ◽  
Author(s):  
Abdelrahman Elshafay ◽  
Ngo Xuan Tinh ◽  
Samar Salman ◽  
Yara Saber Shaheen ◽  
Eman Bashir Othman ◽  
...  
Keyword(s):  
Platelet Aggregation ◽  
Lipid Accumulation ◽  
Clinical Effects ◽  
Anticancer Effects ◽  
Cell Proliferation Inhibition ◽  
Potential Possibility ◽  
Anti Inflammatory ◽  
Apoptotic Activity

Abstract Ginsenoside Rk1 (G-Rk1) is one of the unique components created by processing ginseng at high temperature, in particular of Sun Ginseng (SG). The aim of our study was to systematically review the pharmacological effects of G-Rk1.we conducted our search in eight databases and searched manually to select in vivo and in vitro original studies that provided information about biological pharmaceutical effects of G-Rk1 and were published up to August 2015 with no restriction in language or study design. We retrieved 21 eligible papers out of 121 identified ones. Some studies confirmed the G-Rk1 anticancer effects by investigating “cell viability”, “cell proliferation inhibition”, “apoptotic activity”, “anticancer activity” and “effects of G-Rk1 on G1 phase and autophagy in tumor cells” either alone or in combination with G-Rg5. Others proved that it has anti-platelet aggregation activities and anti-inflammatory effects, enhances cognitive function, reduces lipid accumulation and prevents osteoporosis. In conclusion, G-Rk1 has a significant anti-tumor effect of liver cancer, melanoma, and gastric cancer. Additionally, G-Rk1 has demonstrated as anti-platelet aggregation, anti-inflammatory, and anti-lipid accumulation. All these results corroborate the clinical effects of G-Rk1 and demonstrate the potential possibility to develop G-Rk1-based treatments, either alone or in combination with G-Rg5, with previously mentioned conditions.

scholarly journals ESTIMATION OF ANTI-INFLAMMATORY ACTIVITY AS WELL AS APOPTOTIC ACTIVITY OF ETHANOLIC EXTRACTS OF CROCUS SATIVUS

2019 ◽  
Author(s):  
B. Arirudran ◽  
P. Priyadharshini ◽  
US Mahadeva Rao
Keyword(s):  
Gene Expression ◽  
Oxidative Burst ◽  
Phagocytic Activity ◽  
Vascular Diseases ◽  
Inflammatory Activity ◽  
Crocus Sativus ◽  
Rt Pcr ◽  
Ethanolic Extracts ◽  
Anti Inflammatory ◽  
Apoptotic Activity

Inflammation is a body reaction which embroils cellular and biochemical responses, which is not only symptom for shared diseases but also known to be an initial phase for certain serious Alzheimer’s, cancer, heart vascular diseases. In order to overcome these drawbacks, there is an urgent need for nutraceuticals with excellent anti-inflammatory response with minimum side effects. Aim: An attempt has been made to evaluate the anti-inflammatory activity along with gene expression analysis on ethanolic extracts of Crocus sativus (CSEE). Dried stigmas of C. sativus were analyzed for anti-inflammatory activity by macrophage scavenging assay. In this study, the phagocytic activity of the extract was tested on oxidative burst reduction of macrophages. RT-PCR was performed to analyze the anti-apoptotic gene expression during cell death, as a result of the compound treatment on cancer cells. The CSEE unveiled high phagocytic activity on the oxidative burst reduction, presenting intracellular killing and the enhancement of lysosomal enzyme activity, showing the active degranulation of macrophages. These findings suggest that C. sativus possessed excellent anti-inflammatory as well as apoptotic activities. Hence it was proposed that C. sativus could be exploited against oxidative stress, anti-inflammatory, cancer and ageing therapy to justify their use in traditional medicine as a nutraceutical.

scholarly journals Anti-Inflammatory Activity of a CB2 Selective Cannabinoid Receptor Agonist: Signaling and Cytokines Release in Blood Mononuclear Cells

Molecules ◽  
2021 ◽  
Vol 27 (1) ◽  
pp. 64
Author(s):  
Antonella Capozzi ◽  
Daniela Caissutti ◽  
Vincenzo Mattei ◽  
Francesca Gado ◽  
Stefano Martellucci ◽  
...  
Keyword(s):  
Mononuclear Cells ◽  
Cannabinoid Receptor ◽  
Inflammatory Diseases ◽  
Jurkat Cells ◽  
Parp Cleavage ◽  
Peripheral Blood Mononuclear ◽  
Blood Mononuclear Cells ◽  
Anti Inflammatory ◽  
Apoptotic Activity

The endocannabinoid system (ECS) exerts immunosuppressive effects, which are mostly mediated by cannabinoid receptor 2 (CBR2), whose expression on leukocytes is higher than CBR1, mainly localized in the brain. Targeted CBR2 activation could limit inflammation, avoiding CBR1-related psychoactive effects. Herein, we evaluated in vitro the biological activity of a novel, selective and high-affinity CBR2 agonist, called JT11, studying its potential CBR2-mediated anti-inflammatory effect. Trypan Blue and MTT assays were used to test the cytotoxic and anti-proliferative effect of JT11 in Jurkat cells. Its pro-apoptotic activity was investigated analyzing both cell cycle and poly PARP cleavage. Finally, we evaluated its impact on LPS-induced ERK1/2 and NF-kB-p65 activation, TNF-α, IL-1β, IL-6 and IL-8 release in peripheral blood mononuclear cells (PBMCs) from healthy donors. Selective CB2R antagonist SR144528 and CBR2 knockdown were used to further verify the selectivity of JT11. We confirmed selective CBR2 activation by JT11. JT11 regulated cell viability and proliferation through a CBR2-dependent mechanism in Jurkat cells, exhibiting a mild pro-apoptotic activity. Finally, it reduced LPS-induced ERK1/2 and NF-kB-p65 phosphorylation and pro-inflammatory cytokines release in human PBMCs, proving to possess in vitro anti-inflammatory properties. JT11 as CBR2 ligands could enhance ECS immunoregulatory activity and our results support the view that therapeutic strategies targeting CBR2 signaling could be promising for the treatment of chronic inflammatory diseases.

scholarly journals Protective Effect of Eugenol against Acetaminophen-Induced Hepatotoxicity in Human Hepatocellular Carcinoma Cells via Antioxidant, Anti-Inflammatory, and Anti-Necrotic Potency

2021 ◽  
Vol 9 (A) ◽  
Author(s):  
Florenly Florenly ◽  
Liena Sugianto ◽  
I Nyoman Ehrich Lister ◽  
Ermi Girsang ◽  
Chrismis Novalinda Ginting ◽  
...  
Keyword(s):  
Gene Expression ◽  
Hepg2 Cells ◽  
Hepatoprotective Effect ◽  
Protective Effects ◽  
Cyp 2E1 ◽  
Mediators Of Inflammation ◽  
Tnf Α ◽  
Anti Inflammatory ◽  
Apoptotic Activity ◽  
Factor Α

BACKGROUND: Overdoses acetaminophen (APAP) could cause acute liver failure, even though it used is for analgesics. APAP could cause hepatotoxicity due to multiple mediators of inflammation and oxidative stress. Eugenol has been reported to have anti-inflammatory and antioxidant activity but its hepatoprotective effect has not been widely reported. AIM: The purpose of this research is to know if eugenol could protect HepG2 cells from APAP. METHODS: HepG2 that induced by APAP as hepatotoxicity cells model was treated by using eugenol at 6.25 and 25 μg/mL. The protective effects of eugenol toward hepatotoxicity were evaluated by determine tumor necrosis factor-α (TNF-α) concentration, apoptotic activity, reactive oxygen species (ROS) level, also cytochrome (CYP)2E1 and GPX gene expression. RESULTS: Eugenol at 6.25 and 25 μg/mL concentration can reduce TNF-α concentration, the apoptotic, necrotic, dead cells, and ROS level. Besides it can increase the gene expression (GPX and CYP2E1). The best hepatoprotective effect was found when using the eugenol at 25 μg/mL. CONCLUSION: Therefore, eugenol can be used to protect HepG2 cells against APAP.

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