scholarly journals Plasma membrane is the target of rapid antibacterial action of silver nanoparticles in Escherichia coli and Pseudomonas aeruginosa

2018 ◽  
Vol Volume 13 ◽  
pp. 6779-6790 ◽  
Author(s):  
Olesja M Bondarenko ◽  
Mariliis Sihtmäe ◽  
Julia Kuzmičiova ◽  
Lina Ragelienė ◽  
Anne Kahru ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Pseudomonas Aeruginosa ◽  
Silver Nanoparticles ◽  
Plasma Membrane ◽  
Antibacterial Action

scholarly journals Bacterial plasma membrane is the main cellular target of silver nanoparticles in Escherichia coli and Pseudomonas aeruginosa

2018 ◽  
Author(s):  
Olesja M Bondarenko ◽  
Mariliis Sihtmäe ◽  
Julia Kuzmičiova ◽  
Lina Ragelienė ◽  
Anne Kahru ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Pseudomonas Aeruginosa ◽  
Silver Nanoparticles ◽  
Plasma Membrane ◽  
Antimicrobial Properties ◽  
Consumer Products ◽  
Minimal Bactericidal Concentration ◽  
Flow Cytometry Analysis ◽  
E Coli ◽  
Cellular Target

ABSTRACTSilver nanoparticles (AgNP) are widely used in consumer products, mostly due to their excellent antimicrobial properties. One of the well-established antibacterial mechanisms of AgNP is their efficient contact with bacteria and dissolution on cell membranes. To our knowledge, the primary mechanism of cell wall damage and the event(s) initiating bactericidal action of AgNP are not yet elucidated.In this study we used a combination of different assays to reveal the effect of AgNP on i) bacterial envelope in general, ii) outer membrane (OM) and iii) on plasma membrane (PM). We showed that bacterial PM was the main target of AgNP in Gram-negative bacteria Escherichia coli and Pseudomonas aeruginosa. AgNP depolarized bacterial PM, induced the leakage of the intracellular K+, inhibited respiration and caused the depletion of the intracellular ATP. In contrast, AgNP had no significant effect on the bacterial OM. Most of the adverse effects on bacterial envelope and PM occurred within the seconds and in the concentration range of 7-160 μM AgNP, depending on the bacteria and assay used, while irreversible inhibition of bacterial growth (minimal bactericidal concentration after 1-h exposure of bacteria to AgNP) occurred at 40 μM AgNP for P. aeruginosa and at 320 μM AgNP for E. coli.Flow cytometry analysis showed that AgNP were binding to P. aeruginosa but not to E. coli cells and were found inside the P. aeruginosa cells. Taking into account that AgNP did not damage OM, we speculate that AgNP entered P. aeruginosa via specific mechanism, e.g., transport through porins.

scholarly journals INVESTIGAÇÃO DA ATIVIDADE ANTIBACTERIANA DE Calendula officinalis L. (ASTERACEAE)

2001 ◽  
Vol 2 (1) ◽  
Author(s):  
Ana Marcia De Matos Volpato ◽  
Ester De Moura Rios ◽  
Marilis Dallarmi Miguel ◽  
Paulo Cesar Sander ◽  
Obdúlio Gomes Miguel
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Staphylococcus Epidermidis ◽  
Antibacterial Action ◽  
Dilution Method ◽  
Calendula Officinalis ◽  
Gram Negative ◽  
Solid Media ◽  
Calendula Officinalis L

A atividade antimicrobiana de Calendula officinalis L. (Asteraceae) já foi cientificamente comprovada em outros países. O objetivo deste trabalho foi avaliar a atividade antibacteriana de C. officinalis cultivada no Paraná - Brasil, nas condições de extração etanólica 50%, com filtração e partições sucessivas com hexano, diclorometano, acetato de etila e butanol, frente a cepas Escherichia coli ATCC 25922, Pseudomonas aeruginosa ATCC 27853, Staphylococcus aureus ATCC 25923, Staphylococcus epidermidis ATCC 12228 e Bacillus cereus, pela técnica da difusão em meio sólido por cavidade e posteriormente pelo método de diluição em placa. Os resultados demonstraram que algumas frações do extrato inibiram as bactérias gram-positivas, com atividade frente a Staphylococcus e Bacilos e inexiste em gram negativas, indicando que o princípio ativo responsável pela atividade antibacteriana observada possa ser isolado a partir das frações Hexânica e DCM nas condições de extração propostas neste trabalho. Abstract The Calendula officinalis L. (Asteraceae) antimicrobial action has already been scientifically proved in other countries. The aim of this study was to assess the antibacterial action of the Callendula officinalis which is grown in Paraná - Brasil, under extraction conditions at ethanol 50%, with filtration and successive partitions with hexane, dichloromethane, ethyl acetate and butane, contrasting with strains Escherichia coli ATCC 25922, Pseudomonas aeruginosa ATCC 27853, Staphylococcus aureus ATCC 25923, Staphylococcus epidermidis ATCC 12228 and Bacilus cereus, by the difusion technique in solid media by cavity and afterwards by the plaque dilution method. The results showed that some fractions of the extract inhibited the gram-prositive bacteria, with activity in contrast with Staphylococcus and Bacillus and non-existing in gram-negative, also indicating that the active ingredient responsible for the antibacterial action observed can be isolated from the Hexanic and DCM fractions under the extraction conditions proposed in this study.

scholarly journals Effect of Storage Period on Silver Nanoparticles Biosynthesized By Pseudomonas Aeroginosa

2019 ◽  
Vol 9 (04) ◽  
pp. 678-681
Author(s):  
Ashraf S Hassan ◽  
Khawlah J Khalaf ◽  
Hamzia A Ajah
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Silver Nanoparticles ◽  
Antibacterial Activity ◽  
Candida Albicans ◽  
Storage Period ◽  
Before And After ◽  
Pathogenic Microbes

The present study demonstrates the effect of storage period on silver nanoparticles (AgNPs), which synthesized by Pseudomonas aeruginosa and their antibacterial activity. The result shows that the size of (AgNPs) which synthesis by Pseudomonas aeruginosa was 93.55nm after 4-72hour, and when storage about 2 years, we found that the size of AgNPs was stable and reduced to 69.0nm. Antibacterial activity against pathogenic microbes: Escherichia coli, Pseudomonas aeruginosa, Staphylococcus aureus, Serratia sp,Streptococcus sp , Klebsiella sp, Candida albicans was performed before and after storage and found that AgNPs have activity against this microbes.

scholarly journals Green Synthesis of Silver Nanoparticles Using Novel Launaea Procumbens Leaves Extract and Screening Its Antibacterial Activity

2021 ◽  
Author(s):  
Sanjay Ratan Kumavat ◽  
SATYENDRA MISHRA
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Silver Nanoparticles ◽  
Green Synthesis ◽  
Gram Negative Bacteria ◽  
Zone Of Inhibition ◽  
Gram Negative ◽  
Green Synthesized Silver Nanoparticles ◽  
Launaea Procumbens

Abstract Plants are emerging as a cost-effective and ecofriendly method for green synthesis of nanoparticles. The plant extract Launaea procumbens was used as a reduction agent in the green synthesis of silver nanoparticles. UV-Visible spectroscopy, HR-TEM, SAED, FE-SEM, EDAX, DLS, and FT-IR were used to study the green synthesized silver nanoparticles. UV-Vis spectroscopy of a prepared silver solution revealed maximum absorption at 435 nm. The synthesized silver nanoparticles were found to be spherical in shape with a size in the range of 24.28 to 31.54 nm. DLS analysis was used to determine the size of the green synthesized silver nanoparticles, which showed outstanding antibacterial action against Gram-positive bacteria Bacillus subtilis and Staphylococcus aureus, as well as Gram-negative bacteria Escherichia coli and Pseudomonas aeruginosa. Gram-positive Bacillus subtilis had a maximum zone of inhibition of 20 mm, Staphylococcus aureus had a zone of inhibition of 19 mm, and Gram-negative bacteria Escherichia coli and Pseudomonas aeruginosa had zones of inhibition of 13 mm.

scholarly journals Transparent Nanocrystallite Silver for Antibacterial Coating

2013 ◽  
Vol 2013 ◽  
pp. 1-6 ◽  
Author(s):  
W. Ahliah Ismail ◽  
Zainal Abidin Ali ◽  
R. Puteh
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Antimicrobial Activity ◽  
Silver Nanoparticles ◽  
Sol Gel ◽  
Precipitation Method ◽  
Antibacterial Coating ◽  
Gel Film ◽  
Crystallite Sizes

Transparent sol-gel film with antibacterial coating property incorporating silver nanoparticles has been successfully developed. Silver nanoparticles were synthesized by precipitation method at room temperature. XRD structural studies show that crystallite sizes in the range of 18 nm to 40 nm were produced. The coating system used methyltrimethoxy silane as binder and N-propanol as diluent to obtain the highest transperancy. 2.5% wt of nanosilver crystallites was added as antibacterial agent. The coating mixture was applied onto glass plates using sponges and tested againstStaphylococcus aureus,Escherichia coli, andPseudomonas aeruginosa. Values of antimicrobial activity of 4.6, 7.2, and 4.2 were, respectively, obtained forStaphylococcus aureus,Escherichia coliandPseudomonas Aeruginosa. Coating with antimicrobial activity greater than 2 classified as antibacterial.

Morphological analysis of the antimicrobial action of silver and gold nanoparticles stabilized with ceftriaxone on Escherichia coli using atomic force microscopy

2014 ◽  
Vol 38 (11) ◽  
pp. 5633-5640 ◽  
Author(s):  
Muhammad Raza Shah ◽  
Shujat Ali ◽  
Muhammad Ateeq ◽  
Samina Perveen ◽  
Shakil Ahmed ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Gold Nanoparticles ◽  
Atomic Force Microscopy ◽  
Silver Nanoparticles ◽  
Morphological Analysis ◽  
Antimicrobial Action ◽  
Antibacterial Action ◽  
Gold And Silver Nanoparticles ◽  
Force Microscopy ◽  
Atomic Force

Monitoring the antibacterial action of gold and silver nanoparticlesviaAFM.

Integrated approach to evaluating the toxicity of novel cysteine-capped silver nanoparticles to Escherichia coli and Pseudomonas aeruginosa

The Analyst ◽  
2014 ◽  
Vol 139 (5) ◽  
pp. 954-963 ◽  
Author(s):  
John H. Priester ◽  
Aditi Singhal ◽  
Binghui Wu ◽  
Galen D. Stucky ◽  
Patricia A. Holden
Keyword(s):  
Escherichia Coli ◽  
Pseudomonas Aeruginosa ◽  
Silver Nanoparticles ◽  
Bacterial Growth ◽  
Ag Nanoparticles ◽  
Cell Membranes ◽  
Integrated Approach ◽  
E Coli ◽  
Particle Dissolution ◽  
Measured Particle

The toxicity of cysteine capped Ag nanoparticles toE. coliandP. aeruginosawas evaluated using an integrated approach that measured particle dissolution, bacterial growth, and effects on cell membranes.

Disinfection of Drinking Water from Escherichia coli and Pseudomonas aeruginosa by Using Silver Nanoparticles

2020 ◽  
Vol 1002 ◽  
pp. 478-488
Author(s):  
Raid Salih Jawad ◽  
Alaa Hussein Younus ◽  
Hussein Hafidh Abbas ◽  
Ahmed Shihab ◽  
Aya R. Jawad ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Pseudomonas Aeruginosa ◽  
Silver Nanoparticles ◽  
Drinking Water ◽  
Water Resources ◽  
Antibacterial Effect ◽  
Aqueous Suspension ◽  
Common Species ◽  
Wet Chemistry ◽  
Real Challenge

Due to the lack of raining levels, accretion population growth and increasing pollution in the water resources, the world nowadays is facing a real challenge of providing enough drinking water supplies. Many common species of bacteria could be founded in the water resources like Escherichia coli and Pseudomonas aeruginosa. This paper is an attempt to find an effective agent to eliminate these bacteria. silver nanoparticles (AgNPs) have been tested against these bacteria. Sliver nanoparticles were synthesized by wet chemistry method and were dispersed in an aqueous suspension and prepared in different concentrations. Also, some common antibiotics have been tested against the selected bacteria including; gentamycin (GN), cephalexin (KF), erythromycin (E), ampicillin (AM) and amoxicillin (AMX). The results confirmed that silver nanoparticles had a good antibacterial effect against the selected bacteria in comparison with the tested antibiotics.

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