Hyperbranched polymer nanofibrous membrane grafted with silver nanoparticles for dual antifouling and antibacterial properties against Escherichia coli, Staphylococcus aureus and Pseudomonas aeruginosa

2020 ◽  
Vol 148 ◽  
pp. 104494 ◽  
Author(s):  
Khona Maziya ◽  
Bhekisisa C. Dlamini ◽  
Soraya P. Malinga
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Silver Nanoparticles ◽  
Hyperbranched Polymer ◽  
Antibacterial Properties ◽  
Nanofibrous Membrane

scholarly journals Synthesis of CoFe 2 O 4 /BiOI Nanocomposite: Effects on Staphylococcus aureus, Escherichia coli, Pseudomonas Aeruginosa and Bacillus Cereus

2021 ◽  
Author(s):  
Davood Gheidari ◽  
Morteza Mehrdad ◽  
Saloomeh Maleki ◽  
Samanesadat Hosseini
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Bacillus Cereus ◽  
Antibacterial Properties ◽  
Limited Area ◽  
X Ray Diffraction ◽  
X Ray ◽  
Inhibition Concentration ◽  
Resolution Mapping

Abstract With the increase of general knowledge and the advancement of science and technology, antibacterial substances were used more than antibiotics. In our current study, the antibacterial virtues of CFO/BiOI nanocomposite were investigated due to its high importance on Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa and Bacillus cereus. MIC, MBC , Disk Diffusion and IC50 tests Cefalotin (CF), Amoxicillin (AMX), Gentamicin (GM), Trimethoprim-sulphamethoxazole (SXT) and Ceftriaxone (CRO) antibiotics in concentration 30W, 10i, 10t , 25h and 30 were used to find the antibacterial properties of the synthesized nanocomposite, respectively. For the synthesis of nanocomposites polyethylene glycol (PEG) and sulfonic acid was used as a solvent. It is noteworthy that the synthesis was performed by heat dissolution method without the presence of surfactant. Also, various techniques such as X-Ray Diffraction(XRD), Scanning Electron Microscope (SEM), High resolution mapping and Energy Dispersive X-ray spectroscopy (EDAX) have been used to determine the properties of produced nanocomposites. SEM test results showed that the formed nanoparticles were globular and their size was limited area of 22 to 34 nm. The results showed CFO / BiOI nanocomposite exhibits strong significant biological activity against Bacillus cereus. The results of MBC (Minimum Bactericidal Concentration) and MIC (Minimum Inhibition Concentration) tests for CFO/BiOI nanocomposites on bacteria were examined in the range of 0.12-0.48 mg/ml and 0.06 to 0.24 mg/ml respectively. According to the results, the minimum IC50 value was determined at a concentration of 0.061 mg/ml. On the other hand, the most resisting and susceptible bacteria in this method were Pseudomonas aeruginosa and Bacillus cereus, respectively. These findings are identical to those of a prior study on CoFe2O4 nanoparticles antibacterial properties. MBC of the nanocomposites, 50 µl from all the tubes that showed no obvious bacterial growth were distributed on BHI agar plates and incubated for 24 h at 37 ◦C. The MBC endpoint is defined as the lowest concentration which killed 98% of the bacterial population.

scholarly journals The Study of Antibacterial Properties of Anbarnasara Smoke on Multi-Drug Resistant Bacteria Isolated From Urinary Infection in Pregnant Women

2020 ◽  
Vol 8 (4) ◽  
pp. 110-115
Author(s):  
Afsaneh Molamirzaei ◽  
Maryam Allahdadian ◽  
Monir Doudi
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Pregnant Women ◽  
Urinary Infection ◽  
Antibacterial Properties ◽  
Resistant Bacteria ◽  
Klebsiella Pneumonia ◽  
Drug Resistant

Background: Using smoke from burning donkey dung has been popular in the treatment of many diseases in Iran. Objective: This study aimed to investigating the antimicrobial properties of donkey dung smoke on multi-drug resistant (MDR) bacteria isolated from urinary infection. Materials and Methods: First, 300 and 200 urine samples were collected from pregnant and non-pregnant women in Isfahan, Iran. Then in each group, 100 bacterial isolates including Escherichia coli, Klebsiella pneumonia, Proteus vulgaris, Staphylococcus epidermidis, Staphylococcus aureus, Pseudomonas aeruginosa, and Staphylococcus saprophyticus were isolated. Antibiotic resistant protocol was determined by antibiogram test. Donkey dung was sterilized, disintegrated, and heated. The smokes were concentrated in n-hexane solvent (65%) and were collected after evaporation of the solvent. Finally, the antibacterial activities of the concentrations of 0.25, 0.5 and 1 mg/mL of the smokes were detected using disk diffusion and macrodilution methods. Results: The most abundant MDR isolates causing urinary infections in pregnant and non-pregnant women was Escherichia coli. The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of donkey dung smoke on MDR isolates from pregnant women were 0.25 mg/mL and 0.5 mg/mL, respectively. In the case of MDR isolates in non-pregnant women, the MIC of the smoke on Escherichia coli, Pseudomonas aeruginosa, and Staphylococcus aureus was 0.25 mg/mL, and the MBC on these isolates was 0.5 mg/mL. Conclusion: The smokes from donkey dung investigated in the present study have suitable potentials for controlling the infections after In vivo analysis.

scholarly journals Effect of Storage Period on Silver Nanoparticles Biosynthesized By Pseudomonas Aeroginosa

2019 ◽  
Vol 9 (04) ◽  
pp. 678-681
Author(s):  
Ashraf S Hassan ◽  
Khawlah J Khalaf ◽  
Hamzia A Ajah
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Silver Nanoparticles ◽  
Antibacterial Activity ◽  
Candida Albicans ◽  
Storage Period ◽  
Before And After ◽  
Pathogenic Microbes

The present study demonstrates the effect of storage period on silver nanoparticles (AgNPs), which synthesized by Pseudomonas aeruginosa and their antibacterial activity. The result shows that the size of (AgNPs) which synthesis by Pseudomonas aeruginosa was 93.55nm after 4-72hour, and when storage about 2 years, we found that the size of AgNPs was stable and reduced to 69.0nm. Antibacterial activity against pathogenic microbes: Escherichia coli, Pseudomonas aeruginosa, Staphylococcus aureus, Serratia sp,Streptococcus sp , Klebsiella sp, Candida albicans was performed before and after storage and found that AgNPs have activity against this microbes.

scholarly journals Green Synthesis of Silver Nanoparticles Using Novel Launaea Procumbens Leaves Extract and Screening Its Antibacterial Activity

2021 ◽  
Author(s):  
Sanjay Ratan Kumavat ◽  
SATYENDRA MISHRA
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Silver Nanoparticles ◽  
Green Synthesis ◽  
Gram Negative Bacteria ◽  
Zone Of Inhibition ◽  
Gram Negative ◽  
Green Synthesized Silver Nanoparticles ◽  
Launaea Procumbens

Abstract Plants are emerging as a cost-effective and ecofriendly method for green synthesis of nanoparticles. The plant extract Launaea procumbens was used as a reduction agent in the green synthesis of silver nanoparticles. UV-Visible spectroscopy, HR-TEM, SAED, FE-SEM, EDAX, DLS, and FT-IR were used to study the green synthesized silver nanoparticles. UV-Vis spectroscopy of a prepared silver solution revealed maximum absorption at 435 nm. The synthesized silver nanoparticles were found to be spherical in shape with a size in the range of 24.28 to 31.54 nm. DLS analysis was used to determine the size of the green synthesized silver nanoparticles, which showed outstanding antibacterial action against Gram-positive bacteria Bacillus subtilis and Staphylococcus aureus, as well as Gram-negative bacteria Escherichia coli and Pseudomonas aeruginosa. Gram-positive Bacillus subtilis had a maximum zone of inhibition of 20 mm, Staphylococcus aureus had a zone of inhibition of 19 mm, and Gram-negative bacteria Escherichia coli and Pseudomonas aeruginosa had zones of inhibition of 13 mm.

scholarly journals Transparent Nanocrystallite Silver for Antibacterial Coating

2013 ◽  
Vol 2013 ◽  
pp. 1-6 ◽  
Author(s):  
W. Ahliah Ismail ◽  
Zainal Abidin Ali ◽  
R. Puteh
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Antimicrobial Activity ◽  
Silver Nanoparticles ◽  
Sol Gel ◽  
Precipitation Method ◽  
Antibacterial Coating ◽  
Gel Film ◽  
Crystallite Sizes

Transparent sol-gel film with antibacterial coating property incorporating silver nanoparticles has been successfully developed. Silver nanoparticles were synthesized by precipitation method at room temperature. XRD structural studies show that crystallite sizes in the range of 18 nm to 40 nm were produced. The coating system used methyltrimethoxy silane as binder and N-propanol as diluent to obtain the highest transperancy. 2.5% wt of nanosilver crystallites was added as antibacterial agent. The coating mixture was applied onto glass plates using sponges and tested againstStaphylococcus aureus,Escherichia coli, andPseudomonas aeruginosa. Values of antimicrobial activity of 4.6, 7.2, and 4.2 were, respectively, obtained forStaphylococcus aureus,Escherichia coliandPseudomonas Aeruginosa. Coating with antimicrobial activity greater than 2 classified as antibacterial.

scholarly journals Antibacterial Properties of Nanosilver PLLA Fibrous Membranes

2009 ◽  
Vol 2009 ◽  
pp. 1-5 ◽  
Author(s):  
Lin Li ◽  
Yi Li ◽  
Jiashen Li ◽  
Lei Yao ◽  
Arthur F. T. Mak ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Silver Nanoparticles ◽  
Antibacterial Properties ◽  
Weight Ratio ◽  
Antibacterial Effects ◽  
E Coli ◽  
Fibrous Membranes ◽  
Composite Fibrous Membranes

Nanosilver has been studied as a valuable material for it strong antibacterial effects. In this study, we investigated the antibacterial properties of nano silver Poly-L-Lactic acid (Ag/PLLA) composite fibrous membranes. Ag/PLLA fibrous membranes were prepared with silver nanoparticles having weight ratio of silver nanoparticles to PLLA at 5% (w/w). In vitro antibacterial tests were performed usingEscherichia coli(E. coli) andStaphylococcus aureus(Staph.) to determine the antibacterial capability of the Ag/PLLA fibrous membranes. As the results suggested, Ag/PLLA fibrous membranes showed strong antibacterial properties. Thus, Ag/PLLA fibrous membrane can be used as an antibacterial scaffold for tissue engineering.

scholarly journals Antibacterial properties of quercetin

2017 ◽  
Vol 8 (1) ◽  
Author(s):  
Renu Narendra Jaisinghani
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Lactobacillus Casei ◽  
Shigella Flexneri ◽  
Antibacterial Properties ◽  
Dilution Method ◽  
Proteus Vulgaris ◽  
E Coli ◽  
Broth Dilution

Quercetin is a polyphenolic flavonoid with potential chemoprotective properties. In the present work its antibacterial properties were studied against Staphylococcus aureus, Escherichia coli, Shigella flexneri, Proteus vulgaris, Pseudomonas aeruginosa, Lactobacillus casei var shirota by broth dilution method. Quercetin inhibited S. aureus, P. aeruginosa at concentration 20 mcg/mL while P. vulgaris and E. coli were inhibited at concentration 300 mcg/mL and 400 mcg/mL respectively. Shigella flexneri and Lactobacillus casei var shirota were completely indifferent even at concentration of 500 mcg/mL.

scholarly journals Antibacterial Activity of Aqueous Extracts of Artemisia species against some Pathogenic Bacteria

2017 ◽  
Vol 14 (2) ◽  
pp. 621-624
Author(s):  
Hend A. Alwathnani
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Enterococcus Faecalis ◽  
Pathogenic Bacteria ◽  
Promising Result ◽  
Antibacterial Properties ◽  
Maximum Activity ◽  
Aqueous Extracts ◽  
Artemisia Species

ABSTRACT: The present study was lead with a view to evaluate the antibacterial potentials of aqueous extracts of Artemisia species against human pathogenic bacteria: Escherichia coli (E.coli), Pseudomonas aeruginosa (Ps. aeruginosa) , Staphylococcus aureus (Staph. aureus) and Enterococcus faecalis (Entero. faecalis) by two methods, first method was minimum inhibitory concentration (abbreviation: MIC) and sound method was minimum bactericidal concentration (abbreviation: MBC). Three crude plants extracts namely Artemisia monosperma, Artemisia cina and Artemisia argyi were found to show potential antibacterial properties against the isolated human pathogenic bacterial isolates. The results of MIC and MBC values of Artemisia monosperma for Enterococcus faecalis strains were at least concentration almost 2.3 and 4.1 mg/ml respectively, while MIC and MBC of Artemisia cina for Escherichia coli were highest concentration 3.9 and 4.9 mg/ml respectively. Moreover, MIC and MBC values for Pseudomonas aeruginosa, Staphylococcus aureus and Enterococcus faecalis strains was correspondent with MIC and MBC values of Escherichia coli for aqueous extracts of Artemisia species meanwhile it is Artemisia cina which showed maximum activity against Escherichia coli. Among the Artemisia species tested, Artemisia cina showed the most promising result. Form these results prove the antibacterial potential of the plants and hence provide support for the use of them in traditional medicine.

scholarly journals Bactericidal Properties of Bacillus Subtilis Nanoparticles Against Selected Human Pathogens

2020 ◽  
Vol 1 (5) ◽  
Author(s):  
A. O. Daniels ◽  
J. K. Fadairo ◽  
A. O. Fashoyin
Keyword(s):  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Silver Nanoparticles ◽  
Bacillus Subtilis ◽  
Bacterial Resistance ◽  
Antibacterial Properties ◽  
Resistance Index ◽  
High Rate ◽  
Resistance To Antibiotics ◽  
Antibiotic Susceptibility Test

The use of biologically synthesized nanoparticles has been an area of research interest in recent times. Due to the high rate of bacterial resistance to antibiotics, there is a need to search for a more potent alternative to ineffective antibiotics. This study aims to evaluate the antibacterial effects of silver nanoparticles synthesized by Bacillus subtilis against Pseudomonas aeruginosa and Staphylococcus aureus. Silver nanoparticles were obtained by dissolving 0.842 gram of AgNO3 silver nitrate into 100ml of B. subtilis in Mueller Hinton broth. The antibacterial susceptibility of the nanoparticles formed was carried out using standard methods. Comparative antibacterial test was also carried out using standard antibiotics The multiple antibiotic resistance index were also determined. The zones of inhibition were 29 and 12 mm against Staphylococcus aureus and Pseudomonas aeruginosa respectively after 8 hrs of nanoparticle synthesis. The antibiotic susceptibility test using standard antibiotics revealed that S. aureus was sensitive to only Erythromycin and ofloxacin with a zone of inhibition of 15mm and 9mm respectively while P. aeruginosa was sensitive only to ofloxacin. The Multiple resistance index (MARi) shows P aeruginosa to have MARi of 0.9 while S, aureus has MARi of 0.82. The result indicated that B. subtilis nanoparticles presented better antibacterial properties than standard antibiotic and can be explored as a candidate for drug production to fight bacterial resistance to antibiotics.

Sign in / Sign up

Export Citation Format

Share Document