scholarly journals Probing the intracellular refractive index and molecular interaction of gold nanoparticle in HeLa cells using single particle spectroscopy

2018 ◽  
Vol Volume 13 ◽  
pp. 6019-6028 ◽  
Author(s):  
Abu SM Mohsin ◽  
Mariam B Salim
Keyword(s):  
Refractive Index ◽  
Gold Nanoparticle ◽  
Hela Cells ◽  
Single Particle ◽  
Molecular Interaction ◽  
Single Particle Spectroscopy

scholarly journals The scattering of light by turbid media.–Part II

1931 ◽  
Vol 131 (817) ◽  
pp. 464-475 ◽  
Keyword(s):  
Refractive Index ◽  
Absorption Coefficient ◽  
Spherical Shell ◽  
Direct Observation ◽  
Single Particle ◽  
Unit Volume ◽  
Incident Light ◽  
Parallel Beam ◽  
Photometric Observations ◽  
Transmission And Reflection

In Part I it was shown how the values of the transmission and reflection of a sheet of a medium containing particles in suspension can he calculated. First the amounts of light scattered in the forward and forward directions from a single particle were determined; from these results the transmission 1 and rejection R for diffuse incident light were found for a layer of the disusing medium, when the effects of boundary reflections are negligible. At this stage, the expressions developed apply to a mist or fog consisting of particles suspended in air. Finally it was shown how, if the particles are suspended in some other medium, having a different refractive index from that of air, the transmission and reflection ז and p can be expressed in terms of T and R and the surface rejection coefficients. The more general expressions, for the case when the incident light is a parallel beam, were also developed. We shall now show how the absorption coefficient μ can be determined from photometric observations. As a check on the theory, we shall deduce the diameter D of the particles and the number N present per unit volume and compare these calculated values with those found by direct observation, Finally, the necessary modifications of the theory will be made to cover the case when the diffusing medium is in the form of a spherical shell.

scholarly journals High-precision protein-tracking with interferometric scattering microscopy

2020 ◽  
Author(s):  
Richard W. Taylor ◽  
Cornelia Holler ◽  
Reza Gholami Mahmoodabadi ◽  
Michelle Küppers ◽  
Houman Mirzaalian Dastjerdi ◽  
...  
Keyword(s):  
Epidermal Growth Factor Receptor ◽  
Growth Factor ◽  
Epidermal Growth Factor ◽  
Gold Nanoparticle ◽  
Particle Tracking ◽  
Single Particle ◽  
Growth Factor Receptor ◽  
Single Particle Tracking ◽  
Protein Tracking ◽  
Epidermal Growth

The mobility of proteins and lipids within the cell, sculpted oftentimes by the organisation of the membrane, reveals a great wealth of information on the function and interaction of these molecules as well as the membrane itself. Single particle tracking has proven to be a vital tool to study the mobility of individual molecules and unravel details of their behaviour. Interferometric scattering (iSCAT) microscopy is an emerging technique well suited for visualising the diffusion of gold nanoparticle-labelled membrane proteins to a spatial and temporal resolution beyond the means of traditional fluorescent labels. We discuss the applicability of interferometric single particle tracking (iSPT) microscopy to investigate the minutia in the motion of a protein through measurements visualising the mobility of the epidermal growth factor receptor in various biological scenarios on the live cell.

scholarly journals Probing the variability in oxidation states of magnetite nanoparticles by single-particle spectroscopy

2018 ◽  
Vol 6 (4) ◽  
pp. 875-882 ◽  
Author(s):  
A. Fraile Rodríguez ◽  
C. Moya ◽  
M. Escoda-Torroella ◽  
A. Romero ◽  
A. Labarta ◽  
...  
Keyword(s):  
Oxidation State ◽  
Absorption Spectroscopy ◽  
Magnetite Nanoparticles ◽  
Single Particle ◽  
Cation Distribution ◽  
Oxidation States ◽  
Magnetic Response ◽  
X Ray ◽  
Single Particle Spectroscopy ◽  
X Ray Absorption

Single-particle X-ray absorption spectroscopy reveals that the oxidation state and cation distribution of individual magnetite nanoparticles may be largely heterogeneous even when the macroscopic structural and magnetic response of the ensembles is uniform.

scholarly journals Method to measure the size-resolved real part of aerosol refractive index using differential mobility analyzer in tandem with single-particle soot photometer

2019 ◽  
Vol 12 (7) ◽  
pp. 3541-3550 ◽  
Author(s):  
Gang Zhao ◽  
Weilun Zhao ◽  
Chunsheng Zhao
Keyword(s):  
Refractive Index ◽  
Single Particle ◽  
Radiative Forcing ◽  
Differential Mobility ◽  
Ambient Aerosols ◽  
Aerosol Radiative Forcing ◽  
Abstract Knowledge ◽  
Wide Range ◽  
Aerosol Radiative Effects ◽  
Aerosol Radiative

Abstract. Knowledge on the refractive index of ambient aerosols can help reduce the uncertainties in estimating aerosol radiative forcing. A new method is proposed to retrieve the size-resolved real part of the refractive index (RRI). The main principle of deriving the RRI is measuring the scattering intensity by a single-particle soot photometer (SP2) of size-selected aerosols. This method is validated by a series of calibration experiments using the components of the known RRI. The retrieved size-resolved RRI covers a wide range, from 200 to 450 nm, with uncertainty of less than 0.02. Measurements of the size-resolved RRI can improve the understanding of the aerosol radiative effects.

Single particle technique for one-step homogeneous detection of cancer marker using gold nanoparticle probes

The Analyst ◽  
2011 ◽  
Vol 136 (20) ◽  
pp. 4247 ◽  
Author(s):  
Tao Lan ◽  
Chaoqing Dong ◽  
Xiangyi Huang ◽  
Jicun Ren
Keyword(s):  
Gold Nanoparticle ◽  
Single Particle ◽  
Cancer Marker ◽  
Nanoparticle Probes ◽  
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