Single particle technique for one-step homogeneous detection of cancer marker using gold nanoparticle probes

The Analyst ◽  
2011 ◽  
Vol 136 (20) ◽  
pp. 4247 ◽  
Author(s):  
Tao Lan ◽  
Chaoqing Dong ◽  
Xiangyi Huang ◽  
Jicun Ren
Keyword(s):  
Gold Nanoparticle ◽  
Single Particle ◽  
Cancer Marker ◽  
Nanoparticle Probes ◽  
One Step

Homogeneous Digital Biosensor for Circulating Tumor DNA by Enumerating Dual-color Quantum Dot Complex

The Analyst ◽  
2021 ◽  
Author(s):  
Xiaojun Liu ◽  
Zhangjian Wu ◽  
Xinyi Lin ◽  
Wei Bu ◽  
Lei Qin ◽  
...  
Keyword(s):  
Quantum Dot ◽  
Single Particle ◽  
Triple Helix ◽  
Circulating Tumor Dna ◽  
Cancer Management ◽  
Counting Approach ◽  
Particle Counting ◽  
Dual Color ◽  
One Step ◽  
Tumor Dna

Monitoring ctDNA in blood is important to cancer management. Here, we develop a one-step single particle counting approach for directly quantifying ctDNA in plasma. Hairpin DNA containing a triple helix...

scholarly journals Characterization of Conventional One-Step Sodium Thiosulfate Facilitated Gold Nanoparticle Synthesis

2015 ◽  
Vol 10 (1) ◽  
Author(s):  
Scott-Eugene Saverot ◽  
Laura M Reese ◽  
Daniela Cimini ◽  
Peter J Vikesland ◽  
Lissett Ramirez Bickford
Keyword(s):  
Gold Nanoparticle ◽  
Sodium Thiosulfate ◽  
Nanoparticle Synthesis ◽  
One Step ◽  
Gold Nanoparticle Synthesis

scholarly journals High-precision protein-tracking with interferometric scattering microscopy

2020 ◽  
Author(s):  
Richard W. Taylor ◽  
Cornelia Holler ◽  
Reza Gholami Mahmoodabadi ◽  
Michelle Küppers ◽  
Houman Mirzaalian Dastjerdi ◽  
...  
Keyword(s):  
Epidermal Growth Factor Receptor ◽  
Growth Factor ◽  
Epidermal Growth Factor ◽  
Gold Nanoparticle ◽  
Particle Tracking ◽  
Single Particle ◽  
Growth Factor Receptor ◽  
Single Particle Tracking ◽  
Protein Tracking ◽  
Epidermal Growth

The mobility of proteins and lipids within the cell, sculpted oftentimes by the organisation of the membrane, reveals a great wealth of information on the function and interaction of these molecules as well as the membrane itself. Single particle tracking has proven to be a vital tool to study the mobility of individual molecules and unravel details of their behaviour. Interferometric scattering (iSCAT) microscopy is an emerging technique well suited for visualising the diffusion of gold nanoparticle-labelled membrane proteins to a spatial and temporal resolution beyond the means of traditional fluorescent labels. We discuss the applicability of interferometric single particle tracking (iSPT) microscopy to investigate the minutia in the motion of a protein through measurements visualising the mobility of the epidermal growth factor receptor in various biological scenarios on the live cell.

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