scholarly journals 3D culture of adult mouse neural stem cells within functionalized self-assembling peptide scaffolds

2011 ◽  
pp. 943 ◽  
Author(s):  
Cunha ◽  
Villa ◽  
Silva ◽  
Gelain ◽  
Silvia Panseri
Keyword(s):  
Stem Cells ◽  
Neural Stem Cells ◽  
Adult Mouse ◽  
3D Culture ◽  
Self Assembling ◽  
Peptide Scaffolds

scholarly journals Spatio-Temporal Recruitment Of Adult Neural Stem Cells For Transient Neurogenesis During Pregnancy

2021 ◽  
Author(s):  
Zayna Chaker ◽  
Corina Segalada ◽  
Fiona Doetsch
Keyword(s):  
Stem Cells ◽  
Olfactory Bulb ◽  
Neural Stem Cells ◽  
Adult Mouse ◽  
Life Experiences ◽  
Brain Plasticity ◽  
Perinatal Care ◽  
Adult Mouse Brain ◽  
Spatio Temporal ◽  
Care Period

Neural stem cells (NSCs) in the adult mouse brain contribute to lifelong brain plasticity. NSCs in the adult ventricular-subventricular zone (V-SVZ) are heterogeneous and, depending on their location in the niche, give rise to different subtypes of olfactory bulb interneurons. Here, we show that during pregnancy multiple regionally-distinct NSCs are dynamically recruited at different times. Coordinated temporal activation of these NSC pools generates sequential waves of short-lived olfactory bulb interneuron subtypes that mature in the mother around birth and in the perinatal care period. Concomitant with neuronal addition, oligodendrocyte progenitors also transiently increase in the olfactory bulb. Thus, life experiences, such as pregnancy, can trigger transient neurogenesis and gliogenesis under tight spatial and temporal control, and may provide a novel substrate for brain plasticity in anticipation of temporary physiological demand.

scholarly journals Promotion of Cortical Neurogenesis from the Neural Stem Cells in the Adult Mouse Subcallosal Zone

Stem Cells ◽  
2016 ◽  
Vol 34 (4) ◽  
pp. 888-901 ◽  
Author(s):  
Joo Yeon Kim ◽  
Kyuhyun Choi ◽  
Mohammed R. Shaker ◽  
Ju-Hyun Lee ◽  
Boram Lee ◽  
...  
Keyword(s):  
Stem Cells ◽  
Neural Stem Cells ◽  
Adult Mouse ◽  
Cortical Neurogenesis

scholarly journals Stroke Increases Neural Stem Cells and Angiogenesis in the Neurogenic Niche of the Adult Mouse

PLoS ONE ◽  
2014 ◽  
Vol 9 (12) ◽  
pp. e113972 ◽  
Author(s):  
Rui Lan Zhang ◽  
Michael Chopp ◽  
Cynthia Roberts ◽  
Xianshuang Liu ◽  
Min Wei ◽  
...  
Keyword(s):  
Stem Cells ◽  
Neural Stem Cells ◽  
Adult Mouse ◽  
Neurogenic Niche

scholarly journals Heterotopically transplanted CVO neural stem cells generate neurons and migrate with SVZ cells in the adult mouse brain

2010 ◽  
Vol 475 (1) ◽  
pp. 1-6 ◽  
Author(s):  
Lori B. Bennett ◽  
Jingli Cai ◽  
Grigori Enikolopov ◽  
Lorraine Iacovitti
Keyword(s):  
Stem Cells ◽  
Neural Stem Cells ◽  
Mouse Brain ◽  
Adult Mouse ◽  
Adult Mouse Brain

scholarly journals Direct Lineage Conversion of Adult Mouse Liver Cells and B Lymphocytes to Neural Stem Cells

2014 ◽  
Vol 3 (6) ◽  
pp. 948-956 ◽  
Author(s):  
John P. Cassady ◽  
Ana C. D’Alessio ◽  
Sovan Sarkar ◽  
Vardhan S. Dani ◽  
Zi Peng Fan ◽  
...  
Keyword(s):  
Stem Cells ◽  
Neural Stem Cells ◽  
B Lymphocytes ◽  
Mouse Liver ◽  
Adult Mouse ◽  
Liver Cells ◽  
Direct Lineage Conversion ◽  
Adult Mouse Liver

scholarly journals PPARs Expression in Adult Mouse Neural Stem Cells: Modulation of PPARs during Astroglial Differentiaton of NSC

PPAR Research ◽  
2007 ◽  
Vol 2007 ◽  
pp. 1-10 ◽  
Author(s):  
A. Cimini ◽  
L. Cristiano ◽  
E. Benedetti ◽  
B. D'Angelo ◽  
M. P. Cerù
Keyword(s):  
Cell Cycle ◽  
Stem Cells ◽  
Cell Proliferation ◽  
Transcription Factors ◽  
Neural Stem Cells ◽  
Osteoblast Differentiation ◽  
Adult Mouse ◽  
Cell Type ◽  
Cell Proliferation And Differentiation ◽  
Proliferation And Differentiation

PPAR isotypes are involved in the regulation of cell proliferation, death, and differentiation, with different roles and mechanisms depending on the specific isotype and ligand and on the differentiated, undifferentiated, or transformed status of the cell. Differentiation stimuli are integrated by key transcription factors which regulate specific sets of specialized genes to allow proliferative cells to exit the cell cycle and acquire specialized functions. The main differentiation programs known to be controlled by PPARs both during development and in the adult are placental differentiation, adipogenesis, osteoblast differentiation, skin differentiation, and gut differentiation. PPARs may also be involved in the differentiation of macrophages, brain, and breast. However, their functions in this cell type and organs still awaits further elucidation. PPARs may be involved in cell proliferation and differentiation processes of neural stem cells (NSC). To this aim, in this work the expression of the three PPAR isotypes and RXRs in NSC has been investigated.

scholarly journals Establishment of long-term serum-free culture for lacrimal gland stem cells aiming at lacrimal gland repair

2020 ◽  
Vol 11 (1) ◽  
Author(s):  
Sa Xiao ◽  
Yan Zhang
Keyword(s):  
Stem Cells ◽  
Lacrimal Gland ◽  
Progenitor Cell ◽  
Adult Mouse ◽  
3D Culture ◽  
Phenol Red ◽  
Serum Free ◽  
Ductal Cells ◽  
Cell Based Therapy

Abstract Background Aqueous-deficient dry eye disease (ADDED) resulting from dysfunction of the lacrimal gland (LG) is currently incurable. Although LG stem/progenitor cell-based therapy is considered to be a promising strategy for ADDED patients, the lack of a reliable serum-free culture method to obtain enough lacrimal gland stem cells (LGSCs) and the basic standard of LGSC transplantation are obstacles for further research. Methods Adult mouse LGSCs were cultured in Matrigel-based 3D culture under serum-free culture condition, which contained EGF, FGF10, Wnt3A, and Y-27632. LGSCs were continuously passaged over 40 times every 7 days, and the morphology and cell numbers were recorded. LGSCs were induced to differentiate to ductal cells by reducing Matrigel rigidity, while fetal bovine serum was used for the induction of acinar cells. RT-PCR or qRT-PCR analysis, RNA-sequence analysis, H&E staining, and immunofluorescence were used for characterization and examining the differentiation of LGSCs. LGSCs were allotransplanted into diseased LGs to examine the ability of repairing the damage. The condition of eye orbits was recorded using a camera, the tear production was measured using phenol red-impregnated cotton threads, and the engraftments of LGSCs were examined by immunohistochemistry. Results We established an efficient 3D serum-free culture for adult mouse LGSCs, in which LGSCs could be continuously passaged for long-term expansion. LGSCs cultured from both the healthy and ADDED mouse LGs expressed stem/progenitor cell markers Krt14, Krt5, P63, and nestin, had the potential to differentiate into acinar or ductal-like cells in vitro and could engraft into diseased LGs and relieve symptoms of ADDED after orthotopic injection of LGSCs. Conclusion We successfully established an efficient serum-free culture for adult mouse LGSCs aiming at LG repair for the first time. Our approach provides an excellent theoretical and technical reference for future clinical research for ADDED stem cell therapy.

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