scholarly journals Layer-by-layer DNA films incorporating highly transfecting bioreducible poly(amido amine) and polyethylenimine for sequential gene delivery

2018 ◽  
Vol Volume 13 ◽  
pp. 4943-4960 ◽  
Author(s):  
Lingxiao Xie ◽  
Xiong Ding ◽  
Rachel Budry ◽  
Guangzhao Mao
Keyword(s):  
Gene Delivery ◽  
Layer By Layer ◽  
Dna Films

Boosting transfection efficiency: A systematic study using layer-by-layer based gene delivery platform

2021 ◽  
pp. 112161
Author(s):  
Yana V. Tarakanchikova ◽  
Dmitrii S. Linnik ◽  
Tatiana Mashel ◽  
Albert R. Muslimov ◽  
Sergey Pavlov ◽  
...  
Keyword(s):  
Gene Delivery ◽  
Systematic Study ◽  
Transfection Efficiency ◽  
Layer By Layer ◽  
Delivery Platform

scholarly journals Electrical Field-Assisted Gene Delivery from Polyelectrolyte Multilayers

Polymers ◽  
2020 ◽  
Vol 12 (1) ◽  
pp. 133
Author(s):  
Yu-Che Cheng ◽  
Shu-Lin Guo ◽  
Kun-Da Chung ◽  
Wei-Wen Hu
Keyword(s):  
Gene Delivery ◽  
Electric Field ◽  
Treatment Time ◽  
Transfection Efficiency ◽  
Polyelectrolyte Multilayers ◽  
Aqueous Environment ◽  
Layer By Layer ◽  
Electrical Field ◽  
Lbl Assembly ◽  
Electric Field Treatment

To sustain gene delivery and elongate transgene expression, plasmid DNA and cationic nonviral vectors can be deposited through layer-by-layer (LbL) assembly to form polyelectrolyte multilayers (PEMs). Although these macromolecules can be released for transfection purposes, their entanglement only allows partial delivery. Therefore, how to efficiently deliver immobilized genes from PEMs remains a challenge. In this study, we attempt to facilitate their delivery through the pretreatment of the external electrical field. Multilayers of polyethylenimine (PEI) and DNA were deposited onto conductive polypyrrole (PPy), which were placed in an aqueous environment to examine their release after electric field pretreatment. Only the electric field perpendicular to the substrate with constant voltage efficiently promoted the release of PEI and DNA from PEMs, and the higher potential resulted in the more releases which were enhanced with treatment time. The roughness of PEMs also increased after electric field treatment because the electrical field not only caused electrophoresis of polyelectrolytes and but also allowed electrochemical reaction on the PPy electrode. Finally, the released DNA and PEI were used for transfection. Polyplexes were successfully formed after electric field treatment, and the transfection efficiency was also improved, suggesting that this electric field pretreatment effectively assists gene delivery from PEMs and should be beneficial to regenerative medicine application.

Layer-by-Layer Biomimetic Microgels for 3D Cell Culture and Nonviral Gene Delivery

2021 ◽  
Author(s):  
Bruna G. Carvalho ◽  
Franciele F. Vit ◽  
Hernandes F. Carvalho ◽  
Sang W. Han ◽  
Lucimara G. de la Torre
Keyword(s):  
Cell Culture ◽  
Gene Delivery ◽  
3D Cell Culture ◽  
Nonviral Gene Delivery ◽  
Layer By Layer

Mesenchymal stem cell-derived microvesicles mediate BMP2 gene delivery and enhance bone regeneration

2020 ◽  
Vol 8 (30) ◽  
pp. 6378-6389 ◽  
Author(s):  
Zhuo Liang ◽  
Yue Luo ◽  
Yonggang Lv
Keyword(s):  
Stem Cell ◽  
Gene Delivery ◽  
Mesenchymal Stem Cell ◽  
Bone Regeneration ◽  
Self Assembly ◽  
Bone Repair ◽  
Layer By Layer

Microvesicles–polyethyleneimine/pDNA formed via layer-by-layer self-assembly increase the delivery of hBMP2 plasmids and enhance bone repair.

Layer-by-Layer Self-Assembly Vectors for Gene Delivery

2011 ◽  
Vol 11 (1) ◽  
pp. 58-73 ◽  
Author(s):  
Peng Li ◽  
Na Zhang
Keyword(s):  
Gene Delivery ◽  
Self Assembly ◽  
Layer By Layer

Layer-by-layer assembled PEI-based vector with the upconversion luminescence marker for gene delivery

2018 ◽  
Vol 503 (4) ◽  
pp. 2504-2509 ◽  
Author(s):  
Yaqiong Wang ◽  
Ping Cao ◽  
Shicui Li ◽  
Xiaofeng Zhang ◽  
Jin Hu ◽  
...  
Keyword(s):  
Gene Delivery ◽  
Upconversion Luminescence ◽  
Layer By Layer

scholarly journals Revisiting the Cytotoxicity of Cationic Polyelectrolytes as a Principal Component in Layer-by-Layer Assembly Fabrication

Pharmaceutics ◽  
2021 ◽  
Vol 13 (8) ◽  
pp. 1230
Author(s):  
Ekaterina Naumenko ◽  
Farida Akhatova ◽  
Elvira Rozhina ◽  
Rawil Fakhrullin
Keyword(s):  
Gene Delivery ◽  
Electrostatic Interactions ◽  
Surface Engineering ◽  
Delivery Systems ◽  
In Vitro Cytotoxicity ◽  
Water Soluble ◽  
Layer By Layer ◽  
Dependent Manner ◽  
Cationic Polyelectrolytes

Polycations are an essential part of layer-by-layer (LbL)-assembled drug delivery systems, especially for gene delivery. In addition, they are used for other related applications, such as cell surface engineering. As a result, an assessment of the cytotoxicity of polycations and elucidation of the mechanisms of polycation toxicity is of paramount importance. In this study, we examined in detail the effects of a variety of water-soluble, positively charged synthetic polyelectrolytes on in vitro cytotoxicity, cell and nucleus morphology, and monolayer expansion changes. We have ranked the most popular cationic polyelectrolytes from the safest to the most toxic in relation to cell cultures. 3D cellular cluster formation was disturbed by addition of polyelectrolytes in most cases in a dose-dependent manner. Atomic force microscopy allowed us to visualize in detail the structures of the polyelectrolyte–DNA complexes formed due to electrostatic interactions. Our results indicate a relationship between the structure of the polyelectrolytes and their toxicity, which is necessary for optimization of drug and gene delivery systems.

Sign in / Sign up

Export Citation Format

Share Document