scholarly journals Dissemination Pattern of Multidrug Resistant Carbapenemase Producing Klebsiella pneumoniae Isolates Using Pulsed-Field Gel Electrophoresis in Southwestern Iran

2020 ◽  
Vol Volume 13 ◽  
pp. 921-929 ◽  
Author(s):  
Zahra Hashemizadeh ◽  
Zahra Hosseinzadeh ◽  
Negar Azimzadeh ◽  
Mohammad Motamedifar
Keyword(s):  
Klebsiella Pneumoniae ◽  
Gel Electrophoresis ◽  
Multidrug Resistant ◽  
Pulsed Field Gel Electrophoresis ◽  
Pulsed Field ◽  
Southwestern Iran

scholarly journals Emergence of Multidrug-Resistant Klebsiella pneumoniae Isolates Producing VIM-4 Metallo-β-Lactamase, CTX-M-15 Extended-Spectrum β-Lactamase, and CMY-4 AmpC β-Lactamase in a Tunisian University Hospital

2006 ◽  
Vol 50 (12) ◽  
pp. 4198-4201 ◽  
Author(s):  
Sonia Ktari ◽  
Guillaume Arlet ◽  
Basma Mnif ◽  
Valérie Gautier ◽  
Fouzia Mahjoubi ◽  
...  
Keyword(s):  
Klebsiella Pneumoniae ◽  
Gel Electrophoresis ◽  
Multidrug Resistant ◽  
Pulsed Field Gel Electrophoresis ◽  
Clinical Isolates ◽  
University Hospital ◽  
Class 1 Integron ◽  
Pulsed Field ◽  
Extended Spectrum ◽  
Class 1

ABSTRACT Klebsiella pneumoniae clinical isolates resistant to carbapenems were recovered from 11 patients in the hospital of Sfax, Tunisia. The isolates were closely related as shown by pulsed-field gel electrophoresis, and they produced VIM-4 metallo-enzyme, CTX-M-15 extended-spectrum β-lactamase, and CMY-4 AmpC enzyme. The bla VIM-4 gene is part of a class 1 integron.

Characterization and Antimicrobial Resistance of Salmonella Typhimurium Isolates from Clinically Diseased Pigs in Korea

2016 ◽  
Vol 79 (11) ◽  
pp. 1884-1890 ◽  
Author(s):  
SANG-IK OH ◽  
JONG WAN KIM ◽  
MYEONGJU CHAE ◽  
JI-A JUNG ◽  
BYUNGJAE SO ◽  
...  
Keyword(s):  
Antimicrobial Resistance ◽  
Salmonella Typhimurium ◽  
Resistance Genes ◽  
Gel Electrophoresis ◽  
Salmonella Enteritidis ◽  
Antimicrobial Agents ◽  
Multidrug Resistant ◽  
Pulsed Field Gel Electrophoresis ◽  
Pulsed Field ◽  
Antimicrobial Resistance Genes

ABSTRACT This study investigated the prevalence of Salmonella enterica serovar and antimicrobial resistance in Salmonella Typhimurium isolates from clinically diseased pigs collected from 2008 to 2014 in Korea. Isolates were also characterized according to the presence of antimicrobial resistance genes and pulsed-field gel electrophoresis patterns. Among 94 Salmonella isolates, 81 (86.2%) were identified as being of the Salmonella Typhimurium serotype, followed by Salmonella Derby (6 of 94, 6.4%), Salmonella 4,[5],12:i:− (4 of 94, 4.3%), Salmonella Enteritidis (2 of 94, 2.1%), and Salmonella Brandenburg (1 of 94, 1.1%). The majority of Salmonella Typhimurium isolates were resistant to tetracycline (92.6%), followed by streptomycin (88.9%) and ampicillin (80.2%). Overall, 96.3% of Salmonella Typhimurium isolates showed multidrug-resistant phenotypes and commonly harbored the resistance genes blaTEM (64.9%), flo (32.8%), aadA (55.3%), strA (58.5%), strB (58.5%), sulII (53.2%), and tetA (61.7%). The pulsed-field gel electrophoresis analysis of 45 Salmonella Typhimurium isolates from individual farms revealed 27 distinct patterns that formed one major and two minor clusters in the dendrogram analysis, suggesting that most of the isolates (91.1%) from diseased pigs were genetically related. These findings can assist veterinarians in the selection of appropriate antimicrobial agents to combat Salmonella Typhimurium infections in pigs. Furthermore, they highlight the importance of continuous surveillance of antimicrobial resistance and genetic status in Salmonella Typhimurium for the detection of emerging resistance trends.

scholarly journals Plasmid-Mediated 16S rRNA Methylases in Aminoglycoside-Resistant Enterobacteriaceae Isolates in Shanghai, China

2008 ◽  
Vol 53 (1) ◽  
pp. 271-272 ◽  
Author(s):  
Qiong Wu ◽  
Yibo Zhang ◽  
Lizhong Han ◽  
Jingyong Sun ◽  
Yuxing Ni
Keyword(s):  
Escherichia Coli ◽  
16S Rrna ◽  
Klebsiella Pneumoniae ◽  
Gel Electrophoresis ◽  
Enterobacter Cloacae ◽  
Pulsed Field Gel Electrophoresis ◽  
Pulsed Field ◽  
High Level ◽  
Level Resistance

ABSTRACT High-level resistance to aminoglycosides produced by 16S rRNA methylases in Enterobacteriaceae isolates was investigated. The prevalences of armA in Escherichia coli, Klebsiella pneumoniae, and Enterobacter cloacae were 0.6%, 3.0%, and 10%, respectively. rmtB was more prevalent than armA. Pulsed-field gel electrophoresis patterns indicated that armA and rmtB have spread horizontally and clonally.

scholarly journals Emergence ofKlebsiella pneumoniaeCoproducing KPC-2 and 16S rRNA Methylase ArmA in Poland

2010 ◽  
Vol 55 (1) ◽  
pp. 443-446 ◽  
Author(s):  
Katarzyna Zacharczuk ◽  
Katarzyna Piekarska ◽  
Jolanta Szych ◽  
Elwira Zawidzka ◽  
Agnieszka Sulikowska ◽  
...  
Keyword(s):  
16S Rrna ◽  
Klebsiella Pneumoniae ◽  
Gel Electrophoresis ◽  
Variable Region ◽  
Pulsed Field Gel Electrophoresis ◽  
Epidemic Strain ◽  
Pulsed Field ◽  
Ca 50 ◽  
16S Rrna Methylase

ABSTRACTAKlebsiella pneumoniaeepidemic strain that coproduced carbapenemase KPC-2 (K. pneumoniaecarbapenemase 2) and 16S rRNA methylase ArmA has emerged in Poland. Four nonduplicate isolates from patients in a hospital in Warsaw, Poland, were found to carry theblaKPC-2andarmAgenes on ca. 50-kb and 90-kb plasmids, respectively. Tn4401with a 100-bp deletion in the variable region was detected in all the isolates. XbaI pulsed-field gel electrophoresis (PFGE) revealed 93.2% similarity of the isolates. All the isolates were resistant to carbapenems and 4,6-disubstituted 2-deoxystreptamines.

scholarly journals Clinical Isolates of Enterobacteriaceae Producing Extended-Spectrum β-Lactamases: Prevalence of CTX-M-3 at a Hospital in China

2003 ◽  
Vol 47 (2) ◽  
pp. 790-793 ◽  
Author(s):  
Hui Wang ◽  
Swathi Kelkar ◽  
Weiyuan Wu ◽  
Minjun Chen ◽  
John P. Quinn
Keyword(s):  
Escherichia Coli ◽  
Klebsiella Pneumoniae ◽  
Teaching Hospital ◽  
Gel Electrophoresis ◽  
Enterobacter Cloacae ◽  
Pulsed Field Gel Electrophoresis ◽  
Clinical Isolates ◽  
Citrobacter Freundii ◽  
Pulsed Field ◽  
Extended Spectrum

ABSTRACT The prevalence of extended-spectrum β-lactamase-producing strains was demonstrated in 5 of 44 (11.4%) Escherichia coli, 17 of 43 (39.5%) Klebsiella pneumoniae, 3 of 50 (6.0%) Enterobacter cloacae, and 2 of 25 (8.0%) Citrobacter freundii strains at a teaching hospital in China. Nineteen of these 27 strains expressed CTX-M-3 β-lactamase (pI 8.6). A subset of the clinical isolates expressing the CTX-M-3 enzyme, tested by pulsed-field gel electrophoresis, revealed multiple clones. Five isolates expressed a novel enzyme, SHV-43 (pI 8.0), which had two substitutions (Leu113Phe and Thr149Ser) compared with SHV-1.

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