scholarly journals Plasmid-Mediated 16S rRNA Methylases in Aminoglycoside-Resistant Enterobacteriaceae Isolates in Shanghai, China

2008 ◽  
Vol 53 (1) ◽  
pp. 271-272 ◽  
Author(s):  
Qiong Wu ◽  
Yibo Zhang ◽  
Lizhong Han ◽  
Jingyong Sun ◽  
Yuxing Ni
Keyword(s):  
Escherichia Coli ◽  
16S Rrna ◽  
Klebsiella Pneumoniae ◽  
Gel Electrophoresis ◽  
Enterobacter Cloacae ◽  
Pulsed Field Gel Electrophoresis ◽  
Pulsed Field ◽  
High Level ◽  
Level Resistance

ABSTRACT High-level resistance to aminoglycosides produced by 16S rRNA methylases in Enterobacteriaceae isolates was investigated. The prevalences of armA in Escherichia coli, Klebsiella pneumoniae, and Enterobacter cloacae were 0.6%, 3.0%, and 10%, respectively. rmtB was more prevalent than armA. Pulsed-field gel electrophoresis patterns indicated that armA and rmtB have spread horizontally and clonally.

scholarly journals Clinical Isolates of Enterobacteriaceae Producing Extended-Spectrum β-Lactamases: Prevalence of CTX-M-3 at a Hospital in China

2003 ◽  
Vol 47 (2) ◽  
pp. 790-793 ◽  
Author(s):  
Hui Wang ◽  
Swathi Kelkar ◽  
Weiyuan Wu ◽  
Minjun Chen ◽  
John P. Quinn
Keyword(s):  
Escherichia Coli ◽  
Klebsiella Pneumoniae ◽  
Teaching Hospital ◽  
Gel Electrophoresis ◽  
Enterobacter Cloacae ◽  
Pulsed Field Gel Electrophoresis ◽  
Clinical Isolates ◽  
Citrobacter Freundii ◽  
Pulsed Field ◽  
Extended Spectrum

ABSTRACT The prevalence of extended-spectrum β-lactamase-producing strains was demonstrated in 5 of 44 (11.4%) Escherichia coli, 17 of 43 (39.5%) Klebsiella pneumoniae, 3 of 50 (6.0%) Enterobacter cloacae, and 2 of 25 (8.0%) Citrobacter freundii strains at a teaching hospital in China. Nineteen of these 27 strains expressed CTX-M-3 β-lactamase (pI 8.6). A subset of the clinical isolates expressing the CTX-M-3 enzyme, tested by pulsed-field gel electrophoresis, revealed multiple clones. Five isolates expressed a novel enzyme, SHV-43 (pI 8.0), which had two substitutions (Leu113Phe and Thr149Ser) compared with SHV-1.

scholarly journals Emergence ofKlebsiella pneumoniaeCoproducing KPC-2 and 16S rRNA Methylase ArmA in Poland

2010 ◽  
Vol 55 (1) ◽  
pp. 443-446 ◽  
Author(s):  
Katarzyna Zacharczuk ◽  
Katarzyna Piekarska ◽  
Jolanta Szych ◽  
Elwira Zawidzka ◽  
Agnieszka Sulikowska ◽  
...  
Keyword(s):  
16S Rrna ◽  
Klebsiella Pneumoniae ◽  
Gel Electrophoresis ◽  
Variable Region ◽  
Pulsed Field Gel Electrophoresis ◽  
Epidemic Strain ◽  
Pulsed Field ◽  
Ca 50 ◽  
16S Rrna Methylase

ABSTRACTAKlebsiella pneumoniaeepidemic strain that coproduced carbapenemase KPC-2 (K. pneumoniaecarbapenemase 2) and 16S rRNA methylase ArmA has emerged in Poland. Four nonduplicate isolates from patients in a hospital in Warsaw, Poland, were found to carry theblaKPC-2andarmAgenes on ca. 50-kb and 90-kb plasmids, respectively. Tn4401with a 100-bp deletion in the variable region was detected in all the isolates. XbaI pulsed-field gel electrophoresis (PFGE) revealed 93.2% similarity of the isolates. All the isolates were resistant to carbapenems and 4,6-disubstituted 2-deoxystreptamines.

scholarly journals Outbreak of Infections Caused by Pseudomonas aeruginosa Producing VIM-1 Carbapenemase in Greece

2000 ◽  
Vol 38 (3) ◽  
pp. 1290-1292 ◽  
Author(s):  
Athanassios Tsakris ◽  
Spyros Pournaras ◽  
Neil Woodford ◽  
Marie-France I. Palepou ◽  
Gioia S. Babini ◽  
...  
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Gel Electrophoresis ◽  
Pulsed Field Gel Electrophoresis ◽  
University Hospital ◽  
Pulsed Field ◽  
Serotype O ◽  
Class B ◽  
High Level ◽  
Level Resistance

Resistance to imipenem and meropenem was observed in 211 (16.5%) isolates of Pseudomonas aeruginosa recovered in a Greek university hospital during 1996 to 1998. In six isolates selected from throughout this period, high-level resistance to both carbapenems (MICs ≥ 128 μg/ml) was associated with production of the class B β-lactamase VIM-1. bla VIM-bearing isolates belonged to serotype O:12 and were indistinguishable by pulsed-field gel electrophoresis.

scholarly journals Genotypes, Antibiogram, and Pulsed-Field Gel Electrophoresis Profiles of Escherichia Coli Strains from Piglets in Korea

2009 ◽  
Vol 21 (4) ◽  
pp. 510-516 ◽  
Author(s):  
Su In Lee ◽  
Nabin Rayamahji ◽  
Won Jung Lee ◽  
Seung Bin Cha ◽  
Min Kyung Shin ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Gel Electrophoresis ◽  
Pulsed Field Gel Electrophoresis ◽  
Pulsed Field

scholarly journals Molecular typing of Escherichia coli O157[ratio ]H7 (H−) isolates from cattle in Japan

1999 ◽  
Vol 122 (2) ◽  
pp. 337-341 ◽  
Author(s):  
M. AKIBA ◽  
T. MASUDA ◽  
T. SAMESHIMA ◽  
K. KATSUDA ◽  
M. NAKAZAWA
Keyword(s):  
Escherichia Coli ◽  
Molecular Typing ◽  
Gel Electrophoresis ◽  
Pulsed Field Gel Electrophoresis ◽  
Escherichia Coli O157 ◽  
Outbreak Strain ◽  
Pulsed Field ◽  
E Coli ◽  
Biological Methods ◽  
Pfge Profiles

A total of 77 Escherichia coli O157[ratio ]H7 (H−) isolates from cattle in Japan were investigated by molecular biological methods. Most of these isolates (43 isolates) possessed the stx2 gene, but not stx1. Fifteen bacteriophage types and 50 pulsed-field gel electrophoresis (PFGE) profiles were observed. One isolate was indistinguishable from the human outbreak strain by these methods. This indicates that cattle must be considered as a possible source of human E. coli O157[ratio ]H7 infection in Japan.

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