scholarly journals Eldecalcitol Inhibits LPS-Induced NLRP3 Inflammasome-Dependent Pyroptosis in Human Gingival Fibroblasts by Activating the Nrf2/HO-1 Signaling Pathway

2020 ◽  
Vol Volume 14 ◽  
pp. 4901-4913
Author(s):  
Cancan Huang ◽  
Chaotao Zhang ◽  
Panpan Yang ◽  
Rui Chao ◽  
Ziqi Yue ◽  
...  
Keyword(s):  
Signaling Pathway ◽  
Nlrp3 Inflammasome ◽  
Human Gingival Fibroblasts ◽  
Gingival Fibroblasts

scholarly journals Biological Effects of Shikonin in Human Gingival Fibroblasts via ERK 1/2 Signaling Pathway

Molecules ◽  
2019 ◽  
Vol 24 (19) ◽  
pp. 3542 ◽  
Author(s):  
Kazutaka Imai ◽  
Hirohito Kato ◽  
Yoichiro Taguchi ◽  
Makoto Umeda
Keyword(s):  
Wound Healing ◽  
Signaling Pathway ◽  
Type I Collagen ◽  
Mapk Signaling ◽  
Optimal Concentration ◽  
Human Gingival Fibroblasts ◽  
Type I ◽  
Gingival Fibroblasts ◽  
And Migration ◽  
Proliferation And Migration

Shikonin, an active ingredient of Lithospermum erythrorhizon, exerts anti-inflammatory and antibacterial effects, and promotes wound healing. We investigated whether shikonin stimulated gingival tissue wound healing in human gingival fibroblasts (hGF). In addition, we evaluated the effects of shikonin on the mitogen-activated protein kinase (MAPK) signaling pathway, which has an important role in wound healing. hGF were subjected to primary culture using gingiva collected from patients. The cells were exposed to/treated with Shikonin at concentrations ranging from 0.01 to 100 μM. The optimal concentration was determined by cell proliferation and migration assays. Type I collagen and fibronectin synthesis, the gene expression of vascular endothelial growth factor (VEGF) and FN, and the phosphorylation of Extracellular signal-regulated kinase (ERK) 1/2 were investigated. Identical experiments were performed in the presence of PD98059 our data suggest, a specific ERK 1/2 inhibitor. Shikonin significantly promoted hGF proliferation and migration. Shikonin (1 µM) was chosen as the optimal concentration. Shikonin promoted type I collagen and FN synthesis, increased VEGF and FN expression, and induced ERK 1/2 phosphorylation. These changes were partially suppressed by PD98059. In conclusion, Shikonin promoted the proliferation, migration, type I collagen and FN synthesis, and expression of VEGF and FN via ERK 1/2 signaling pathway in hGFs. Therefore, shikonin may promote periodontal tissue wound healing.

scholarly journals Effect of phenytoin on PI3K/Akt signaling pathway in human gingival fibroblasts

2020 ◽  
Vol 93 (0) ◽  
pp. 1-P-083
Author(s):  
Hiroko Matsumoto ◽  
Reiri Takeuchi ◽  
Junichi Yamane ◽  
Hitoshi Nishimura ◽  
Masamichi Komiya
Keyword(s):  
Signaling Pathway ◽  
Akt Signaling ◽  
Human Gingival Fibroblasts ◽  
Gingival Fibroblasts ◽  
Akt Signaling Pathway
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