scholarly journals Biological Effects of Shikonin in Human Gingival Fibroblasts via ERK 1/2 Signaling Pathway

Molecules ◽  
2019 ◽  
Vol 24 (19) ◽  
pp. 3542 ◽  
Author(s):  
Kazutaka Imai ◽  
Hirohito Kato ◽  
Yoichiro Taguchi ◽  
Makoto Umeda
Keyword(s):  
Wound Healing ◽  
Signaling Pathway ◽  
Type I Collagen ◽  
Mapk Signaling ◽  
Optimal Concentration ◽  
Human Gingival Fibroblasts ◽  
Type I ◽  
Gingival Fibroblasts ◽  
And Migration ◽  
Proliferation And Migration

Shikonin, an active ingredient of Lithospermum erythrorhizon, exerts anti-inflammatory and antibacterial effects, and promotes wound healing. We investigated whether shikonin stimulated gingival tissue wound healing in human gingival fibroblasts (hGF). In addition, we evaluated the effects of shikonin on the mitogen-activated protein kinase (MAPK) signaling pathway, which has an important role in wound healing. hGF were subjected to primary culture using gingiva collected from patients. The cells were exposed to/treated with Shikonin at concentrations ranging from 0.01 to 100 μM. The optimal concentration was determined by cell proliferation and migration assays. Type I collagen and fibronectin synthesis, the gene expression of vascular endothelial growth factor (VEGF) and FN, and the phosphorylation of Extracellular signal-regulated kinase (ERK) 1/2 were investigated. Identical experiments were performed in the presence of PD98059 our data suggest, a specific ERK 1/2 inhibitor. Shikonin significantly promoted hGF proliferation and migration. Shikonin (1 µM) was chosen as the optimal concentration. Shikonin promoted type I collagen and FN synthesis, increased VEGF and FN expression, and induced ERK 1/2 phosphorylation. These changes were partially suppressed by PD98059. In conclusion, Shikonin promoted the proliferation, migration, type I collagen and FN synthesis, and expression of VEGF and FN via ERK 1/2 signaling pathway in hGFs. Therefore, shikonin may promote periodontal tissue wound healing.

scholarly journals Platelet-rich Fibrin Influences on Proliferation and Migration of Human Gingival Fibroblasts

2016 ◽  
Vol 5 (2) ◽  
pp. 83-88
Author(s):  
Thuy Anh Vu Pham ◽  
Hao TT Nguyen ◽  
My TN Nguyen ◽  
Van NL Trinh ◽  
Nga Y Tran ◽  
...  
Keyword(s):  
Wound Healing ◽  
Human Gingival Fibroblasts ◽  
Human Gingival Fibroblast ◽  
Complete Medium ◽  
Gingival Fibroblast ◽  
Gingival Fibroblasts ◽  
Platelet Rich Fibrin ◽  
Liquid Extract ◽  
And Migration ◽  
Proliferation And Migration

ABSTRACT Aims Our study focused on the fabrication of platelet-rich fibrin (PRF) and evaluated its influences on cell behaviors, including proliferation and migration. Materials and methods Platelet-rich fibrin was prepared from human peripheral blood according to Choukroun's method without using nonanticoagulant and foreign factors for platelet activation. Platelet-rich fibrin architecture was studied by hematoxylin and eosin staining. The investigation of PRF effects on human gingival fibroblasts (hGFs) was conducted via PRF liquid extract. Cell proliferation was determined via the number of cells after a period of time incubated in PRF liquid extract. Influence of PRF liquid extract on the migration of hGFs was conducted via scratch wound healing assay. Results Histological staining reviewed the natural fibrin fiber matrix of PRF. Platelet-rich fibrin liquid extract promoted hGF proliferation after 7 days of cultivation. Human gingival fibroblast proliferation in PRF liquid extract was more superior than those cultured in complete medium. Platelet-rich fibrin was also found to be able to promote the migration of hGFs for up to 48 hours. Conclusion These results indicated that PRF is suitable to be used as autologous natural biomaterial in supporting wound healing and in further application in periodontitis treatments. How to cite this article Nguyen HTT, Nguyen MTN, Trinh VNL, Tran NY, Ngo LTQ, Pham TAV, Tran HLB. Platelet-rich Fibrin Influences on Proliferation and Migration of Human Gingival Fibroblasts. Int J Experiment Dent Sci 2016;5(2):83-88.

scholarly journals Novel Pyrazolo[3,4-b] Pyridine Derivative (HLQ2g) Attenuates Hypoxic Pulmonary Hypertension via Restoring cGKI Expression and BMP Signaling Pathway

2021 ◽  
Vol 12 ◽  
Author(s):  
Lijun Li ◽  
Minghui Yin ◽  
Liqing Hu ◽  
Xiaoting Tian ◽  
Xiangrong He ◽  
...  
Keyword(s):  
Pulmonary Hypertension ◽  
Pulmonary Artery ◽  
Right Ventricular ◽  
Signaling Pathway ◽  
Systolic Pressure ◽  
Bmp Signaling ◽  
Pyridine Derivative ◽  
Type I ◽  
And Migration ◽  
Proliferation And Migration

Pulmonary hypertension (PH) is an extremely serious cardiopulmonary disease, finally leading to progressive right ventricular failure and death. Our previous studies have nominated HLQ2g, a pyrazolo[3,4-b] pyridine derivative stimulating soluble guanylate cyclase (sGC), as a new candidate for the treatment of PH, but the specific mechanism is still not clear. The PH model induced by hypoxia was established in rats. Right ventricular systolic pressure (RVSP) was assessed by jugular vein catheterization. RV weight was the index to evaluate RV hypertrophy. The protein levels of cGMP-dependent protein kinase type I (cGKI), bone morphogenetic protein receptor 2 (BMPR2), phosphorylated Smad1/5/8 (p-Smad1/5/8), and inhibitor of differention 1 (Id1) in pulmonary artery and human pulmonary artery smooth muscle cells (HPASMCs) were determined by western blotting. Cell proliferation and migration were evaluated. In the whole experiment, the first clinically available sGC stimulator Riociguat was used as the reference. In hypoxic PH rat model, elevated RVSP and RV hypertrophy were significantly reduced by HLQ2g treatment. Both Riociguat and HLQ2g attenuated vascular remodeling accompanied with up-regulated cGKI expression and BMP signaling pathway, which was characterized by elevated expression of BMPR2, p-Smad1/5/8, and Id1 in HPH rats. In addition, HLQ2g inhibited proliferation and migration of HPASMCs induced by hypoxia and platelet-derived growth factor (PDGF), restored BMPR2 signaling, which was recalled by Rp-8-Br-PET-cGMPS, the inhibitor of cGKI. In summary, the novel pyrazolo[3,4-b] pyridine derivative HLQ2g can alleviate HPH progression by up-regulating cGKI protein and BMP signaling pathway.

Effect of radiation on cytokines, MMP-1 and type I collagen mRNA expressions in human gingival fibroblasts.

2011 ◽  
Vol 29 (15_suppl) ◽  
pp. e16034-e16034
Author(s):  
G. Yavas ◽  
C. Yavas ◽  
B. Bozkurt ◽  
O. Ata ◽  
S. Hakki
Keyword(s):  
Type I Collagen ◽  
Human Gingival Fibroblasts ◽  
Type I ◽  
Gingival Fibroblasts ◽  
Collagen Mrna

Effects of HEMA on type I collagen protein in human gingival fibroblasts

2007 ◽  
Vol 23 (5) ◽  
pp. 313-322 ◽  
Author(s):  
M. Falconi ◽  
G. Teti ◽  
M. Zago ◽  
S. Pelotti ◽  
L. Breschi ◽  
...  
Keyword(s):  
Type I Collagen ◽  
Human Gingival Fibroblasts ◽  
Type I ◽  
Gingival Fibroblasts ◽  
Collagen Protein
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