scholarly journals Assessment of a New Ginsenoside Rh2 Nanoniosomal Formulation for Enhanced Antitumor Efficacy on Prostate Cancer: An in vitro Study

2020 ◽  
Vol Volume 14 ◽  
pp. 3315-3324 ◽  
Author(s):  
Hadi Zare-Zardini ◽  
Ashraf Alemi ◽  
Asghar Taheri-Kafrani ◽  
Seyed Ahmad Hosseini ◽  
Hossein Soltaninejad ◽  
...  
Keyword(s):  
Prostate Cancer ◽  
In Vitro Study ◽  
Vitro Study ◽  
Antitumor Efficacy ◽  
Ginsenoside Rh2

scholarly journals In Vitro Study of Calcium Microsecond Electroporation of Prostate Adenocarcinoma Cells

Molecules ◽  
2020 ◽  
Vol 25 (22) ◽  
pp. 5406 ◽  
Author(s):  
Aleksander Kiełbik ◽  
Wojciech Szlasa ◽  
Olga Michel ◽  
Anna Szewczyk ◽  
Mounir Tarek ◽  
...  
Keyword(s):  
Prostate Cancer ◽  
Cancer Cells ◽  
Calcium Binding ◽  
In Vitro Study ◽  
Vitro Study ◽  
Dynamics Simulation ◽  
Confocal Laser ◽  
Prostate Cancer Cells ◽  
Voltage Pulses

Electroporation, applied as a non-thermal ablation method has proven to be effective for focal prostate treatment. In this study, we performed pre-clinical research, which aims at exploring the specific impact of this so-called calcium electroporation on prostate cancer. First, in an in-vitro study of DU 145 cell lines, microsecond electroporation (μsEP) parameters were optimized. We determined hence the voltage that provides both high permeability and viability of these prostate cancer cells. Subsequently, we compared the effect of μsEP on cells’ viability with and without calcium administration. For high-voltage pulses, the cell death’s mechanism was evaluated using flow-cytometry and confocal laser microscopy. For lower-voltage pulses, the influence of electroporation on prostate cancer cell mobility was studied using scratch assays. Additionally, we applied calcium-binding fluorescence dye (Fluo-8) to observe the calcium uptake dynamic with the fluorescence microscopy. Moreover, the molecular dynamics simulation visualized the process of calcium ions inflow during μsEP. According to our results calcium electroporation significantly decreases the cells viability by promoting apoptosis. Furthermore, our data shows that the application of pulsed electric fields disassembles the actin cytoskeleton and influences the prostate cancer cells’ mobility.

scholarly journals Efficacy of photochemical internalisation using disulfonated chlorin and porphyrin photosensitisers: An in vitro study in 2D and 3D prostate cancer models

2017 ◽  
Vol 393 ◽  
pp. 68-75 ◽  
Author(s):  
Alejandra Martinez de Pinillos Bayona ◽  
Josephine H. Woodhams ◽  
Hayley Pye ◽  
Rifat A. Hamoudi ◽  
Caroline M. Moore ◽  
...  
Keyword(s):  
Prostate Cancer ◽  
In Vitro Study ◽  
Vitro Study ◽  
Cancer Models ◽  
2D And 3D

Novel combination therapy of prostate cancer cells with arsenic trioxide and flutamide: An in-vitro study

2022 ◽  
Vol 74 ◽  
pp. 101684
Author(s):  
Akram Mirzaei ◽  
Mohammad Reza Akbari ◽  
Seyed Saeed Tamehri Zadeh ◽  
Fatemeh Khatami ◽  
Rahil Mashhadi ◽  
...  
Keyword(s):  
Prostate Cancer ◽  
Combination Therapy ◽  
Cancer Cells ◽  
Arsenic Trioxide ◽  
In Vitro Study ◽  
Vitro Study ◽  
Prostate Cancer Cells
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