scholarly journals Antitumor effect of dendritic cells transfected with prostate-specific membrane antigen recombinant adenovirus on prostate cancer: An in vitro study

2016 ◽  
Vol 13 (3) ◽  
pp. 2124-2134 ◽  
Author(s):  
FAN-DONG MENG ◽  
SHUAI WANG ◽  
YOU-HONG JIANG ◽  
CHENG-GUANG SUI
Keyword(s):  
Prostate Cancer ◽  
Dendritic Cells ◽  
Antitumor Effect ◽  
Recombinant Adenovirus ◽  
In Vitro Study ◽  
Vitro Study ◽  
Prostate Specific Membrane Antigen ◽  
Specific Membrane

scholarly journals In Vitro Study of Calcium Microsecond Electroporation of Prostate Adenocarcinoma Cells

Molecules ◽  
2020 ◽  
Vol 25 (22) ◽  
pp. 5406 ◽  
Author(s):  
Aleksander Kiełbik ◽  
Wojciech Szlasa ◽  
Olga Michel ◽  
Anna Szewczyk ◽  
Mounir Tarek ◽  
...  
Keyword(s):  
Prostate Cancer ◽  
Cancer Cells ◽  
Calcium Binding ◽  
In Vitro Study ◽  
Vitro Study ◽  
Dynamics Simulation ◽  
Confocal Laser ◽  
Prostate Cancer Cells ◽  
Voltage Pulses

Electroporation, applied as a non-thermal ablation method has proven to be effective for focal prostate treatment. In this study, we performed pre-clinical research, which aims at exploring the specific impact of this so-called calcium electroporation on prostate cancer. First, in an in-vitro study of DU 145 cell lines, microsecond electroporation (μsEP) parameters were optimized. We determined hence the voltage that provides both high permeability and viability of these prostate cancer cells. Subsequently, we compared the effect of μsEP on cells’ viability with and without calcium administration. For high-voltage pulses, the cell death’s mechanism was evaluated using flow-cytometry and confocal laser microscopy. For lower-voltage pulses, the influence of electroporation on prostate cancer cell mobility was studied using scratch assays. Additionally, we applied calcium-binding fluorescence dye (Fluo-8) to observe the calcium uptake dynamic with the fluorescence microscopy. Moreover, the molecular dynamics simulation visualized the process of calcium ions inflow during μsEP. According to our results calcium electroporation significantly decreases the cells viability by promoting apoptosis. Furthermore, our data shows that the application of pulsed electric fields disassembles the actin cytoskeleton and influences the prostate cancer cells’ mobility.

scholarly journals Features of follicular dendritic cells in ovine pharyngeal tonsil: An in vivo and in vitro study in the context of scrapie pathogenesis

2011 ◽  
Vol 141 (1-2) ◽  
pp. 26-32 ◽  
Author(s):  
Vinciane Toppets ◽  
Valerie Defaweux ◽  
Joelle Piret ◽  
Nathalie Kirschvink ◽  
Luc Grobet ◽  
...  
Keyword(s):  
Dendritic Cells ◽  
In Vitro Study ◽  
Vitro Study ◽  
Follicular Dendritic Cells ◽  
Follicular Dendritic ◽  
Pharyngeal Tonsil

An in vitro study on the antitumor effect of sonodynamic therapy using sinoporphyrin sodium on human glioblastoma cells

Ultrasonics ◽  
2021 ◽  
Vol 110 ◽  
pp. 106272
Author(s):  
Yuanyuan Shen ◽  
Yiling Chen ◽  
Yongpeng Huang ◽  
Xiaojun Zeng ◽  
Lanhui Huang ◽  
...  
Keyword(s):  
Antitumor Effect ◽  
In Vitro Study ◽  
Vitro Study ◽  
Glioblastoma Cells ◽  
Sonodynamic Therapy ◽  
Human Glioblastoma ◽  
Human Glioblastoma Cells

scholarly journals Efficacy of photochemical internalisation using disulfonated chlorin and porphyrin photosensitisers: An in vitro study in 2D and 3D prostate cancer models

2017 ◽  
Vol 393 ◽  
pp. 68-75 ◽  
Author(s):  
Alejandra Martinez de Pinillos Bayona ◽  
Josephine H. Woodhams ◽  
Hayley Pye ◽  
Rifat A. Hamoudi ◽  
Caroline M. Moore ◽  
...  
Keyword(s):  
Prostate Cancer ◽  
In Vitro Study ◽  
Vitro Study ◽  
Cancer Models ◽  
2D And 3D

Interactions between osteosarcoma cell lines and dendritic cells immune function: An in vitro study

2008 ◽  
Vol 253 (1-2) ◽  
pp. 71-80 ◽  
Author(s):  
Michela Muraro ◽  
Oana M. Mereuta ◽  
Francesco Saglio ◽  
Francesca Carraro ◽  
Massimo Berger ◽  
...  
Keyword(s):  
Dendritic Cells ◽  
Immune Function ◽  
Cell Lines ◽  
In Vitro Study ◽  
Vitro Study ◽  
Osteosarcoma Cell
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